MY202375A - Recombinant terpene synthase - Google Patents
Recombinant terpene synthaseInfo
- Publication number
- MY202375A MY202375A MYPI2019000052A MYPI2019000052A MY202375A MY 202375 A MY202375 A MY 202375A MY PI2019000052 A MYPI2019000052 A MY PI2019000052A MY PI2019000052 A MYPI2019000052 A MY PI2019000052A MY 202375 A MY202375 A MY 202375A
- Authority
- MY
- Malaysia
- Prior art keywords
- linalool
- nerolidol
- recombinant
- nucleic acid
- host cell
- Prior art date
Links
- 108010087432 terpene synthase Proteins 0.000 title abstract 4
- CDOSHBSSFJOMGT-UHFFFAOYSA-N linalool Chemical compound CC(C)=CCCC(C)(O)C=C CDOSHBSSFJOMGT-UHFFFAOYSA-N 0.000 abstract 10
- FQTLCLSUCSAZDY-UHFFFAOYSA-N (+) E(S) nerolidol Natural products CC(C)=CCCC(C)=CCCC(C)(O)C=C FQTLCLSUCSAZDY-UHFFFAOYSA-N 0.000 abstract 5
- 239000001490 (3R)-3,7-dimethylocta-1,6-dien-3-ol Substances 0.000 abstract 5
- CDOSHBSSFJOMGT-JTQLQIEISA-N (R)-linalool Natural products CC(C)=CCC[C@@](C)(O)C=C CDOSHBSSFJOMGT-JTQLQIEISA-N 0.000 abstract 5
- FQTLCLSUCSAZDY-ATGUSINASA-N Nerolidol Chemical compound CC(C)=CCC\C(C)=C\CC[C@](C)(O)C=C FQTLCLSUCSAZDY-ATGUSINASA-N 0.000 abstract 5
- 229930007744 linalool Natural products 0.000 abstract 5
- WASNIKZYIWZQIP-AWEZNQCLSA-N nerolidol Natural products CC(=CCCC(=CCC[C@@H](O)C=C)C)C WASNIKZYIWZQIP-AWEZNQCLSA-N 0.000 abstract 5
- 210000004027 cell Anatomy 0.000 abstract 4
- 102000039446 nucleic acids Human genes 0.000 abstract 4
- 108020004707 nucleic acids Proteins 0.000 abstract 4
- 150000007523 nucleic acids Chemical class 0.000 abstract 4
- 108090000623 proteins and genes Proteins 0.000 abstract 3
- 235000004094 Coleus amboinicus Nutrition 0.000 abstract 1
- 241000588724 Escherichia coli Species 0.000 abstract 1
- 241001184136 Plectranthus amboinicus Species 0.000 abstract 1
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 1
- 230000001588 bifunctional effect Effects 0.000 abstract 1
- 210000001236 prokaryotic cell Anatomy 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
Abstract
The present invention relates to a bifunctional 5 recombinant terpene synthase enzyme which is able to biosynthesize linalool and nerolidol separately or simultaneously and the method of producing thereof. The recombinant terpene synthase having a gene sequence as set forth in SEQ ID No: 2 to produce linalool and nerolidol in a controlled environment. Specifically, a nucleic acid segment comprising nucleic acid encoding terpene synthase specifically linalool/nerolidol synthase protein is isolated from Plectranthus amboinicus. The nucleic acid segment which is PamTps1 gene encodes the amino acid sequence of SEQ ID NO: 1 and the nucleic acid segment is positioned under the control of a promoter to produce a recombinant vector. The recombinant vector expresses linalool/nerolidol synthase upon introduction into a host cell to produce recombinant host cell wherein the host cell is a prokaryotic cell, i.e. Escherichia coli. The recombinant host cell produces linalool and nerolidol simultaneously once cultured under specific conditions.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MYPI2019000052A MY202375A (en) | 2019-01-14 | 2019-01-14 | Recombinant terpene synthase |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MYPI2019000052A MY202375A (en) | 2019-01-14 | 2019-01-14 | Recombinant terpene synthase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MY202375A true MY202375A (en) | 2024-04-24 |
Family
ID=90885672
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MYPI2019000052A MY202375A (en) | 2019-01-14 | 2019-01-14 | Recombinant terpene synthase |
Country Status (1)
| Country | Link |
|---|---|
| MY (1) | MY202375A (en) |
-
2019
- 2019-01-14 MY MYPI2019000052A patent/MY202375A/en unknown
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