MX2021006297A - Celdas de flujo. - Google Patents

Celdas de flujo.

Info

Publication number
MX2021006297A
MX2021006297A MX2021006297A MX2021006297A MX2021006297A MX 2021006297 A MX2021006297 A MX 2021006297A MX 2021006297 A MX2021006297 A MX 2021006297A MX 2021006297 A MX2021006297 A MX 2021006297A MX 2021006297 A MX2021006297 A MX 2021006297A
Authority
MX
Mexico
Prior art keywords
polymeric hydrogel
cationic
flow cells
substrate
flow cell
Prior art date
Application number
MX2021006297A
Other languages
English (en)
Inventor
Tarun Kumar Khurana
Bernard Hirschbein
Yasaman Farshchi
Xi- Jun CHEN
Yir- Shyuan WU
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of MX2021006297A publication Critical patent/MX2021006297A/es

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    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
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    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
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    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
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    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F220/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical or a salt, anhydride ester, amide, imide or nitrile thereof
    • C08F220/02—Monocarboxylic acids having less than ten carbon atoms; Derivatives thereof
    • C08F220/52—Amides or imides
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    • C08F220/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical or a salt, anhydride ester, amide, imide or nitrile thereof
    • C08F220/02—Monocarboxylic acids having less than ten carbon atoms; Derivatives thereof
    • C08F220/52—Amides or imides
    • C08F220/54—Amides, e.g. N,N-dimethylacrylamide or N-isopropylacrylamide
    • C08F220/60—Amides, e.g. N,N-dimethylacrylamide or N-isopropylacrylamide containing nitrogen in addition to the carbonamido nitrogen
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    • C08F8/00—Chemical modification by after-treatment
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    • C08F8/00—Chemical modification by after-treatment
    • C08F8/40—Introducing phosphorus atoms or phosphorus-containing groups
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    • C08J9/00—Working-up of macromolecular substances to porous or cellular articles or materials; After-treatment thereof
    • C08J9/36—After-treatment
    • C08J9/365—Coating
    • C—CHEMISTRY; METALLURGY
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
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    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/069—Absorbents; Gels to retain a fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
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    • C08J2205/00—Foams characterised by their properties
    • C08J2205/02—Foams characterised by their properties the finished foam itself being a gel or a gel being temporarily formed when processing the foamable composition
    • C08J2205/022—Hydrogel, i.e. a gel containing an aqueous composition
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    • C08J2333/00—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides, or nitriles thereof; Derivatives of such polymers
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    • C12Q2523/00—Reactions characterised by treatment of reaction samples
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/629—Detection means characterised by use of a special device being a microfluidic device

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Polymers & Plastics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Clinical Laboratory Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Dispersion Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Materials Engineering (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)

Abstract

Un ejemplo de una celda de flujo incluye un sustrato y un hidrogel polimérico catiónico en el sustrato. El hidrogel polimérico catiónico incluye una entidad catiónica que i) se integra en una unidad monomérica de un hidrogel polimérico inicial o ii) se acopla a la unidad monomérica del hidrogel polimérico inicial a través de un enlazador. La celda de flujo incluye además un cebador de amplificación acoplado al hidrogel polimérico catiónico.
MX2021006297A 2019-08-01 2020-07-22 Celdas de flujo. MX2021006297A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962881597P 2019-08-01 2019-08-01
PCT/US2020/043057 WO2021021515A1 (en) 2019-08-01 2020-07-22 Flow cells

Publications (1)

Publication Number Publication Date
MX2021006297A true MX2021006297A (es) 2021-09-08

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ID=74230094

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Application Number Title Priority Date Filing Date
MX2021006297A MX2021006297A (es) 2019-08-01 2020-07-22 Celdas de flujo.

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US (1) US20220080415A1 (es)
EP (1) EP4007655A4 (es)
JP (1) JP2022541867A (es)
KR (1) KR20220041037A (es)
CN (1) CN113302311A (es)
AU (1) AU2020323880A1 (es)
BR (1) BR112021012873A2 (es)
CA (1) CA3123021A1 (es)
IL (1) IL284066A (es)
MX (1) MX2021006297A (es)
TW (1) TWI802810B (es)
WO (1) WO2021021515A1 (es)

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KR20240004501A (ko) * 2021-04-26 2024-01-11 일루미나, 인코포레이티드 플로우 셀
WO2024081563A1 (en) * 2022-10-10 2024-04-18 Illumina, Inc. Photo-switchable chemistry for reversible hydrogels and reusable flow cells
US20240209429A1 (en) * 2022-12-07 2024-06-27 Illumina, Inc. Monoclonal clustering using double stranded dna size exclusion with patterned seeding
WO2024130202A1 (en) * 2022-12-16 2024-06-20 Illumina, Inc. Flow cells and methods

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US7217542B2 (en) * 2002-10-31 2007-05-15 Hewlett-Packard Development Company, L.P. Microfluidic system for analyzing nucleic acids
US7094330B2 (en) * 2002-12-02 2006-08-22 Epocal Inc. Heterogeneous membrane electrodes
WO2009024869A2 (en) * 2007-03-26 2009-02-26 Owe Orwar Methods and devices for controlled monolayer formation
WO2012027832A1 (en) * 2010-08-30 2012-03-08 The Governors Of The University Of Alberta Setting of multiple priming oligonucleotides for solid gel amplification in hydrogels
US9012022B2 (en) * 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
KR101420837B1 (ko) * 2012-10-09 2014-07-17 재단법인대구경북과학기술원 수화겔 및 이의 제조 방법
US20150267196A1 (en) * 2012-10-12 2015-09-24 Case Western Reserve University Biodegradable hydrogel for polynucleotide delivery
WO2014106493A1 (en) * 2013-01-07 2014-07-10 Anpac Bio-Medical Science (Lishui) Co., Ltd. Apparatus for improved disease detection
US20160054304A1 (en) * 2013-03-14 2016-02-25 Galapagos Nv Molecular targets and compounds, and methods to identify the same, useful in the treatment of fibrotic diseases
KR101492051B1 (ko) * 2013-07-08 2015-02-16 아주대학교산학협력단 양이온성 물질과 음이온성 물질의 정전기적 인력에 의해 제조되는 하이드로겔 및 이의 제조방법
NL2019044B1 (en) * 2017-05-11 2018-11-15 Illumina Inc Protective surface coatings for flow cells
WO2019126040A1 (en) * 2017-12-21 2019-06-27 Illumina, Inc. Flow cells with hydrogel coating

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Publication number Publication date
WO2021021515A1 (en) 2021-02-04
EP4007655A1 (en) 2022-06-08
US20220080415A1 (en) 2022-03-17
AU2020323880A1 (en) 2021-06-17
KR20220041037A (ko) 2022-03-31
EP4007655A4 (en) 2023-09-13
JP2022541867A (ja) 2022-09-28
IL284066A (en) 2021-08-31
CA3123021A1 (en) 2021-02-04
BR112021012873A2 (pt) 2022-02-08
CN113302311A (zh) 2021-08-24
TW202120694A (zh) 2021-06-01
TWI802810B (zh) 2023-05-21

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