WO2019013991A3 - Nucleic acid extraction materials, systems, and methods - Google Patents

Nucleic acid extraction materials, systems, and methods Download PDF

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Publication number
WO2019013991A3
WO2019013991A3 PCT/US2018/040113 US2018040113W WO2019013991A3 WO 2019013991 A3 WO2019013991 A3 WO 2019013991A3 US 2018040113 W US2018040113 W US 2018040113W WO 2019013991 A3 WO2019013991 A3 WO 2019013991A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid extraction
systems
methods
monomer
Prior art date
Application number
PCT/US2018/040113
Other languages
French (fr)
Other versions
WO2019013991A2 (en
Inventor
Brian D. Mather
Cyril Delattre
Tarun Kumar Khurana
Yir-Shyuan WU
Pallavi DAGGUMATI
Behnam Javanmardi
Filiz Gorpe-Yasar
Sebastien Georg Gabriel RICOULT
Xavier VON HATTEN
Daniel Leonard Fuller
Original Assignee
Illumina, Inc.
Illumina Cambridge Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina, Inc., Illumina Cambridge Limited filed Critical Illumina, Inc.
Priority to EP18832414.9A priority Critical patent/EP3652314B1/en
Priority to US16/626,221 priority patent/US11390864B2/en
Priority to CN201880044478.0A priority patent/CN111094564A/en
Priority to DK18832414.9T priority patent/DK3652314T3/en
Publication of WO2019013991A2 publication Critical patent/WO2019013991A2/en
Publication of WO2019013991A3 publication Critical patent/WO2019013991A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F226/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a single or double bond to nitrogen or by a heterocyclic ring containing nitrogen
    • C08F226/06—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a single or double bond to nitrogen or by a heterocyclic ring containing nitrogen by a heterocyclic ring containing nitrogen
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F226/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a single or double bond to nitrogen or by a heterocyclic ring containing nitrogen
    • C08F226/06—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a single or double bond to nitrogen or by a heterocyclic ring containing nitrogen by a heterocyclic ring containing nitrogen
    • C08F226/08—N-Vinyl-pyrrolidine
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F226/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a single or double bond to nitrogen or by a heterocyclic ring containing nitrogen
    • C08F226/06—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a single or double bond to nitrogen or by a heterocyclic ring containing nitrogen by a heterocyclic ring containing nitrogen
    • C08F226/10—N-Vinyl-pyrrolidone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads

Abstract

An example of a nucleic acid extraction material includes a substrate. The nucleic acid extraction material also includes a polycation bonded to at least a portion of a surface of the substrate. The polycation consists of a polymer of a quaternized monomer selected from the group consisting of a quaternized 1-vinylimidazole monomer and a quaternized dimethylaminoethyl methacrylate monomer, or a copolymer of a neutral monomer and the quaternized monomer.
PCT/US2018/040113 2017-07-12 2018-06-28 Nucleic acid extraction materials, systems, and methods WO2019013991A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP18832414.9A EP3652314B1 (en) 2017-07-12 2018-06-28 Nucleic acid extraction materials and methods
US16/626,221 US11390864B2 (en) 2017-07-12 2018-06-28 Nucleic acid extraction materials, systems, and methods
CN201880044478.0A CN111094564A (en) 2017-07-12 2018-06-28 Nucleic acid extraction materials, systems and methods
DK18832414.9T DK3652314T3 (en) 2017-07-12 2018-06-28 NUCLEIC ACID EXTRACTION MATERIALS AND METHODS

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US201762531751P 2017-07-12 2017-07-12
US62/531,751 2017-07-12
US201762539876P 2017-08-01 2017-08-01
US201762539890P 2017-08-01 2017-08-01
US62/539,890 2017-08-01
US62/539,876 2017-08-01

Publications (2)

Publication Number Publication Date
WO2019013991A2 WO2019013991A2 (en) 2019-01-17
WO2019013991A3 true WO2019013991A3 (en) 2019-02-21

Family

ID=65001426

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2018/040113 WO2019013991A2 (en) 2017-07-12 2018-06-28 Nucleic acid extraction materials, systems, and methods

Country Status (5)

Country Link
US (1) US11390864B2 (en)
EP (1) EP3652314B1 (en)
CN (1) CN111094564A (en)
DK (1) DK3652314T3 (en)
WO (1) WO2019013991A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021113287A1 (en) * 2019-12-04 2021-06-10 Illumina, Inc. Preparation of dna sequencing libraries for detection of dna pathogens in plasma
US11918990B2 (en) * 2020-06-17 2024-03-05 Entegris, Inc. Ion-exchange membranes, filters, and methods
CN117467658A (en) * 2022-07-22 2024-01-30 友康生物科技(北京)股份有限公司 Method and device for extracting and detecting nucleic acid

Citations (6)

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US5374522A (en) * 1986-03-20 1994-12-20 Gen-Probe Incorporated Method for releasing RNA and DNA from cells
US5582988A (en) * 1994-09-15 1996-12-10 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
US20090275486A1 (en) * 2006-05-16 2009-11-05 Nurith Kurn Nucleic acid separation and purification method based on reversible charge interactions
US20140054172A1 (en) * 2011-02-10 2014-02-27 Biocule (Scotland) Limited Two-dimensional gel electrophoresis apparatus and method
US20140093909A1 (en) * 2004-09-23 2014-04-03 Tripath Imaging, Inc. Polycationic quaternary ammonium polymer coatings for immobilizing biological samples
WO2016007755A1 (en) * 2014-07-09 2016-01-14 Skog Johan Karl Olov Methods for isolating microvesicles and extracting nucleic acids from biological samples

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DE3211309A1 (en) 1982-03-26 1983-09-29 Metin Dipl.-Ing. 6100 Darmstadt Colpan CHROMATOGRAPHIC METHOD FOR INSULATING MACROMOLECULES
DE3639949A1 (en) 1986-11-22 1988-06-09 Diagen Inst Molekularbio METHOD FOR SEPARATING LONG CHAIN NUCLEIC ACIDS
US6129828A (en) 1996-09-06 2000-10-10 Nanogen, Inc. Apparatus and methods for active biological sample preparation
US6168948B1 (en) * 1995-06-29 2001-01-02 Affymetrix, Inc. Miniaturized genetic analysis systems and methods
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WO2003074571A2 (en) * 2002-02-28 2003-09-12 Keck Graduate Institute Cationic polyelectrolytes in biomolecule purification and analysis
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CA2662401A1 (en) * 2006-09-15 2008-03-20 Basf Se Ampholytic copolymer based on quaternized nitrogen-containing monomers
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Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5374522A (en) * 1986-03-20 1994-12-20 Gen-Probe Incorporated Method for releasing RNA and DNA from cells
US5582988A (en) * 1994-09-15 1996-12-10 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
US20140093909A1 (en) * 2004-09-23 2014-04-03 Tripath Imaging, Inc. Polycationic quaternary ammonium polymer coatings for immobilizing biological samples
US20090275486A1 (en) * 2006-05-16 2009-11-05 Nurith Kurn Nucleic acid separation and purification method based on reversible charge interactions
US20140054172A1 (en) * 2011-02-10 2014-02-27 Biocule (Scotland) Limited Two-dimensional gel electrophoresis apparatus and method
WO2016007755A1 (en) * 2014-07-09 2016-01-14 Skog Johan Karl Olov Methods for isolating microvesicles and extracting nucleic acids from biological samples

Non-Patent Citations (1)

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DAME, G. ET AL.: "Development of a Fast miRNA Extraction System for Tumor Analysis Based on a Simple Lab on Chip Approach", PROCEDIA ENGINEERING, vol. 120, 2015, pages 158 - 162, XP029268338, DOI: doi:10.1016/j.proeng.2015.08.593 *

Also Published As

Publication number Publication date
EP3652314A2 (en) 2020-05-20
WO2019013991A2 (en) 2019-01-17
EP3652314A4 (en) 2021-03-10
US20200325467A1 (en) 2020-10-15
US11390864B2 (en) 2022-07-19
CN111094564A (en) 2020-05-01
DK3652314T3 (en) 2023-09-18
EP3652314B1 (en) 2023-08-23

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