MX2020004017A - Control de patogenos fungicos utilizando la estrategia basada en arni. - Google Patents

Control de patogenos fungicos utilizando la estrategia basada en arni.

Info

Publication number
MX2020004017A
MX2020004017A MX2020004017A MX2020004017A MX2020004017A MX 2020004017 A MX2020004017 A MX 2020004017A MX 2020004017 A MX2020004017 A MX 2020004017A MX 2020004017 A MX2020004017 A MX 2020004017A MX 2020004017 A MX2020004017 A MX 2020004017A
Authority
MX
Mexico
Prior art keywords
rnai
fungal pathogens
based strategy
controlling fungal
controlling
Prior art date
Application number
MX2020004017A
Other languages
English (en)
Inventor
Hailing Jin
Original Assignee
Univ California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ California filed Critical Univ California
Publication of MX2020004017A publication Critical patent/MX2020004017A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]

Abstract

La presente invención se refiere a plantas resistentes a los patógenos. En un aspecto, se proporcionan plantas que comprenden un casete de expresión heterólogo, en donde el casete de expresión comprende un polinucleótido que inhibe la expresión de un gen patógeno fúngico y en el que la planta tiene mayor resistencia a un patógeno fúngico o múltiples patógenos en comparación con una planta de control que carece del casete de expresión. En otro aspecto, poner en contacto una planta o una parte de la planta con ARN bicatenarios o sRNA pequeños que inhiben la expresión de un gen o genes objetivo fúngicos de múltiples patógenos, en donde la planta tiene mayor resistencia a un patógeno o múltiples patógenos en comparación con las plantas de control que no se ha contactado con los ARN. También se proporcionan métodos para fabricar y cultivar plantas resistentes a los patógenos.
MX2020004017A 2017-10-17 2018-10-04 Control de patogenos fungicos utilizando la estrategia basada en arni. MX2020004017A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762573546P 2017-10-17 2017-10-17
PCT/US2018/054412 WO2019079044A1 (en) 2017-10-17 2018-10-04 CONTROL OF FUNGAL PATHOGENS USING AN ARNI-BASED STRATEGY

Publications (1)

Publication Number Publication Date
MX2020004017A true MX2020004017A (es) 2020-07-22

Family

ID=66173836

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2020004017A MX2020004017A (es) 2017-10-17 2018-10-04 Control de patogenos fungicos utilizando la estrategia basada en arni.

Country Status (8)

Country Link
US (1) US11746355B2 (es)
EP (1) EP3697921A4 (es)
CN (1) CN111225980B (es)
AU (1) AU2018350793A1 (es)
BR (1) BR112020006416A2 (es)
CA (1) CA3077067A1 (es)
MX (1) MX2020004017A (es)
WO (1) WO2019079044A1 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112695035B (zh) * 2021-01-22 2022-03-22 浙江理工大学 一种RNA抑菌剂miRNA157d-3p及作物病菌抑制剂
AU2022294075A1 (en) * 2021-06-17 2024-01-25 The Regents Of The University Of California Vesicle formulations for delivery of antifungal nucleic acids
CN113528518B (zh) * 2021-06-28 2022-07-15 西南大学 抑制核盘菌的miRNA及其应用
CN115125324B (zh) * 2022-06-22 2023-05-05 海南大学 一种用于评估被白粉菌侵染的叶片中病原菌rna比例的引物及方法
CN116515889A (zh) * 2023-05-15 2023-08-01 北京林业大学 一种植物叶组织特异性表达dna调控元件及其应用
CN116426541B (zh) * 2023-06-06 2023-09-01 中国农业科学院生物技术研究所 防治作物黄萎病的靶标基因区段、dsRNA及纳米农药组合物

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7709454B2 (en) * 1997-06-20 2010-05-04 New York University Methods and compositions for inhibiting tumorigenesis
EP1781795B1 (en) * 2004-06-30 2011-01-19 Pioneer-Hi-Bred International, Inc. Methods of protecting plants from pathogenic fungi
CN101203611B (zh) * 2005-04-19 2013-08-14 巴斯福植物科学有限公司 控制基因表达的改良方法
DE102006029129A1 (de) * 2006-06-22 2007-12-27 Kws Saat Ag Pathogen induzierbarer synthetischer Promotor
WO2009108389A1 (en) * 2008-02-29 2009-09-03 Rutgers, The State University Disease resistant transgenic plants
US10119148B2 (en) * 2013-10-02 2018-11-06 The Regents Of The University Of California RNAs from pathogens inhibit plant immunity
WO2016176324A1 (en) * 2015-04-27 2016-11-03 The Regents Of The University Of California CONTROLLING FUNGAL PATHOGENS BY DISABLING THEIR SMALL RNA PATHWAYS USING RNAi-BASED STRATEGY
US10724049B2 (en) * 2014-07-24 2020-07-28 The Regents Of The University Of California Controlling fungal pathogens by disabling their small RNA pathways using RNAi-based strategy

Also Published As

Publication number Publication date
EP3697921A4 (en) 2021-11-24
US11746355B2 (en) 2023-09-05
CN111225980A (zh) 2020-06-02
CN111225980B (zh) 2024-05-03
WO2019079044A1 (en) 2019-04-25
CA3077067A1 (en) 2019-04-25
BR112020006416A2 (pt) 2020-09-29
US20200340003A1 (en) 2020-10-29
EP3697921A1 (en) 2020-08-26
AU2018350793A1 (en) 2020-04-09

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