MX2017010311A - Metodos de produccion y recuperacion de rebaudiosido m biosintetico. - Google Patents

Metodos de produccion y recuperacion de rebaudiosido m biosintetico.

Info

Publication number
MX2017010311A
MX2017010311A MX2017010311A MX2017010311A MX2017010311A MX 2017010311 A MX2017010311 A MX 2017010311A MX 2017010311 A MX2017010311 A MX 2017010311A MX 2017010311 A MX2017010311 A MX 2017010311A MX 2017010311 A MX2017010311 A MX 2017010311A
Authority
MX
Mexico
Prior art keywords
steviol glycosides
rebaudioside
recovery methods
biosynthetic production
soluble
Prior art date
Application number
MX2017010311A
Other languages
English (en)
Inventor
Markosyan Avetik
Original Assignee
Purecircle Usa Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Purecircle Usa Inc filed Critical Purecircle Usa Inc
Publication of MX2017010311A publication Critical patent/MX2017010311A/es

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52—Adding ingredients
    • A23L2/56—Flavouring or bittering agents
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/02—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52—Adding ingredients
    • A23L2/54—Mixing with gases
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52—Adding ingredients
    • A23L2/60—Sweeteners
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30—Artificial sweetening agents
    • A23L27/33—Artificial sweetening agents containing sugars or derivatives
    • A23L27/36—Terpene glycosides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/1074—Cyclomaltodextrin glucanotransferase (2.4.1.19)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Seasonings (AREA)

Abstract

Se proporcionan varios procesos de recuperación para la recuperación completa de glicosidos de esteviol de baja solubilidad obtenidos en microorganismos recombinantes. Los glicosidos de esteviol de a-glicosilo solubles se recuperaron completamente en el procesamiento corriente abajo y luego se convirtieron a glicosidos de esteviol por hidrolasas. Los glicosidos de esteviol obtenidos se purificaron y se utilizaron como edulcorantes, potenciadores de dulzura, potenciadores de sabor, y modificadores de sabor en alimentos, bebidas cosméticos y productos farmacéuticos.
MX2017010311A 2015-02-10 2016-02-10 Metodos de produccion y recuperacion de rebaudiosido m biosintetico. MX2017010311A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562114134P 2015-02-10 2015-02-10
PCT/US2016/017236 WO2016130609A1 (en) 2015-02-10 2016-02-10 Rebaudioside m biosynthetic production and recovery methods

Publications (1)

Publication Number Publication Date
MX2017010311A true MX2017010311A (es) 2017-12-14

Family

ID=56615701

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2017010311A MX2017010311A (es) 2015-02-10 2016-02-10 Metodos de produccion y recuperacion de rebaudiosido m biosintetico.

Country Status (5)

Country Link
EP (1) EP3256008A4 (es)
CN (1) CN107404911A (es)
BR (1) BR112017017207B1 (es)
MX (1) MX2017010311A (es)
WO (1) WO2016130609A1 (es)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10517321B2 (en) 2015-07-10 2019-12-31 Sweet Green Fields USA LLC Compositions of steviol multiglycosylated derivatives and stevia components
WO2017009294A1 (en) 2015-07-10 2017-01-19 Dsm Ip Assets B.V. Steviol glycoside composition
CA3003163A1 (en) 2015-10-29 2017-05-04 Senomyx, Inc. High intensity sweeteners
JPWO2017171023A1 (ja) * 2016-03-31 2018-04-12 サントリーホールディングス株式会社 ヘスペリジンの沈殿が抑制された飲料
US10085472B2 (en) * 2016-08-29 2018-10-02 Pepsico, Inc. Compositions comprising rebaudioside J
CN106554983A (zh) * 2016-10-28 2017-04-05 江南大学 甜菊糖杜克苷a的制备方法
EP3592138A4 (en) 2017-03-08 2020-11-25 PureCircle USA Inc. CULTIVARS OF STEVIA PLANTS WITH HIGH REBAUDIOSIDE M CONTENT AND THEIR PRODUCTION PROCESSES
CN106866757B (zh) * 2017-03-16 2020-06-26 诸城市浩天药业有限公司 甜菊糖m苷晶型及制备方法和用途
SG11201910136RA (en) 2017-05-03 2019-11-28 Firmenich Incorporated Methods for making high intensity sweeteners
US20210076719A1 (en) * 2018-02-26 2021-03-18 Firmenich Sa Composition comprising glucosylated terpene glycosides, terpene glycosides and cyclodextrine
CN108707163B (zh) * 2018-06-11 2020-08-14 江西师范大学 一种甜菊醇甙元的制备方法
KR20220151205A (ko) * 2020-03-13 2022-11-14 아미리스 인코퍼레이티드 리바우디오사이드 m 감미료 조성물

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4219571A (en) 1978-06-15 1980-08-26 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo Process for producing a sweetener
US20090162487A1 (en) * 2007-12-21 2009-06-25 The Concentrate Manufacturing Company Of Ireland Beverage products and flavor systems having a non-sweetening amount of rebaudioside a
KR100888694B1 (ko) * 2008-09-01 2009-03-16 김경재 감미질이 우수한 효소처리스테비아 제조방법
US20140030381A1 (en) * 2011-02-17 2014-01-30 Purecircle Usa Inc. Glucosyl stevia composition
US9795156B2 (en) * 2011-03-17 2017-10-24 E.P.C (Beijing) Plant Pharmaceutical Technology Co., Ltd Rebaudioside B and derivatives
WO2013022989A2 (en) * 2011-08-08 2013-02-14 Evolva Sa Recombinant production of steviol glycosides
WO2014122227A2 (en) 2013-02-06 2014-08-14 Evolva Sa Methods for improved production of rebaudioside d and rebaudioside m
MY184253A (en) * 2013-02-11 2021-03-29 Evolva Sa Efficient production of steviol glycosides in recombinant hosts

Also Published As

Publication number Publication date
WO2016130609A1 (en) 2016-08-18
EP3256008A1 (en) 2017-12-20
CN107404911A (zh) 2017-11-28
BR112017017207B1 (pt) 2022-10-18
EP3256008A4 (en) 2018-10-31
BR112017017207A2 (pt) 2018-04-03

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