MX2016017029A - Enriquecimiento de emparejamiento genetico y reaccion en cadena de la polimerasa para la deteccion rapida de microorganismos. - Google Patents

Enriquecimiento de emparejamiento genetico y reaccion en cadena de la polimerasa para la deteccion rapida de microorganismos.

Info

Publication number
MX2016017029A
MX2016017029A MX2016017029A MX2016017029A MX2016017029A MX 2016017029 A MX2016017029 A MX 2016017029A MX 2016017029 A MX2016017029 A MX 2016017029A MX 2016017029 A MX2016017029 A MX 2016017029A MX 2016017029 A MX2016017029 A MX 2016017029A
Authority
MX
Mexico
Prior art keywords
gene
enrichment
matched
microorganisms
chain reaction
Prior art date
Application number
MX2016017029A
Other languages
English (en)
Inventor
M Ozanch Richard
R Hutchison Janine
D Victry Kristin
M Hess Becky
Original Assignee
Battelle Memorial Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Battelle Memorial Institute filed Critical Battelle Memorial Institute
Publication of MX2016017029A publication Critical patent/MX2016017029A/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Se describe un procedimiento para amplificar y detectar microorganismos, tales como especies de Listeria. El procedimiento utiliza medios de enriquecimiento de emparejamiento genético y detección basada en PCR. El medio de enriquecimiento es un medio gastado que se produce usando un microorganismo modificado que contiene una pluralidad de mutaciones en un gen seleccionado, de tal manera que el microorganismo modificado no contiene la signatura de PCR. Por lo tanto, la PCR solo detecta el microorganismo de interés amplificado, no el microorganismo modificado. Se describen procedimientos a modo de ejemplo y kits para la amplificación y detección de especies de Listeria.
MX2016017029A 2014-06-18 2015-06-17 Enriquecimiento de emparejamiento genetico y reaccion en cadena de la polimerasa para la deteccion rapida de microorganismos. MX2016017029A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462013998P 2014-06-18 2014-06-18
PCT/US2015/036231 WO2015195802A1 (en) 2014-06-18 2015-06-17 Gene-matched enrichment and polymerase chain reaction for rapid detection of microorganisms

Publications (1)

Publication Number Publication Date
MX2016017029A true MX2016017029A (es) 2017-09-28

Family

ID=54869106

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2016017029A MX2016017029A (es) 2014-06-18 2015-06-17 Enriquecimiento de emparejamiento genetico y reaccion en cadena de la polimerasa para la deteccion rapida de microorganismos.

Country Status (5)

Country Link
US (2) US10100369B2 (es)
BR (1) BR112016029907A2 (es)
CA (1) CA2952808A1 (es)
MX (1) MX2016017029A (es)
WO (1) WO2015195802A1 (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107164540A (zh) * 2017-07-12 2017-09-15 河北科技师范学院 一种牡蛎中单核细胞增生李斯特氏菌的快速检测方法
EP3765597A4 (en) * 2018-03-14 2022-01-12 Hongwei D. Yu BACTERIAL CULTURES AND PROCESSES FOR PRODUCTION OF ALGINATE
CN109799201B (zh) * 2018-12-29 2020-01-07 芯视界（北京）科技有限公司 农产品农残快速检测方法以及检测用层叠体
CN114245711A (zh) * 2019-08-06 2022-03-25 新诺有限公司 用于异种移植的供体猪
CN111778272A (zh) * 2020-07-16 2020-10-16 河南大学 一种快速检测食源性致病菌沙门氏菌用标准化质粒冻干粉
CN112592985B (zh) * 2020-10-12 2022-05-10 南开大学 一种蜡样芽孢杆菌实时荧光pcr检测方法及应用
CN113462798A (zh) * 2021-07-26 2021-10-01 重庆大学 快速检测金黄色葡萄球菌、沙门氏菌或/和志贺氏菌的lamp引物及方法
CN115992063B (zh) * 2022-08-29 2023-08-18 甘肃省科学院生物研究所 一种抗盐碱复合微生物菌剂、制备方法及应用
CN116286934A (zh) * 2022-12-23 2023-06-23 江南大学 恶臭假单胞菌中冷休克蛋白调控有机化合物耐受性的研究方法
CN116004869A (zh) * 2022-12-30 2023-04-25 圣湘生物科技股份有限公司 检测溶血葡萄球菌、阴沟肠杆菌和肺炎链球菌的组合物

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7935804B2 (en) * 2006-03-01 2011-05-03 Aduro Biotech Engineered Listeria and methods of use thereof

Also Published As

Publication number Publication date
US10100369B2 (en) 2018-10-16
US11104966B2 (en) 2021-08-31
WO2015195802A1 (en) 2015-12-23
US20190002962A1 (en) 2019-01-03
US20150368696A1 (en) 2015-12-24
CA2952808A1 (en) 2015-12-23
BR112016029907A2 (pt) 2017-10-31

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