MX2016007032A - Novedoso sistema de alteracion del genoma para microorganismos. - Google Patents
Novedoso sistema de alteracion del genoma para microorganismos.Info
- Publication number
- MX2016007032A MX2016007032A MX2016007032A MX2016007032A MX2016007032A MX 2016007032 A MX2016007032 A MX 2016007032A MX 2016007032 A MX2016007032 A MX 2016007032A MX 2016007032 A MX2016007032 A MX 2016007032A MX 2016007032 A MX2016007032 A MX 2016007032A
- Authority
- MX
- Mexico
- Prior art keywords
- microorganisms
- microorganism
- methods
- alteration system
- target gene
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
La invención se refiere a un conjunto de construcciones de direccionamiento, que comprende una primera construcción que comprende un sitio de reconocimiento para una endonucleasa, una primera región de homología con un gen objetivo de un microorganismo, y una primera parte de un marcador de selección, y una segunda construcción que comprende una segunda parte del marcador de selección, una segunda región de homología con el gen objetivo del microorganismo, y una copia del sitio de reconocimiento de endonucleasa. La invención se refiere además a métodos para alterar un gen objetivo en un microorganismo, a métodos para producir un microorganismo, y a los microorganismos que son producidos por los métodos de la invención.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NL2011912A NL2011912C2 (en) | 2013-12-06 | 2013-12-06 | Novel genome alteration system for microorganisms. |
| PCT/NL2014/050839 WO2015084178A1 (en) | 2013-12-06 | 2014-12-08 | Novel genome alteration system for microorganisms |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2016007032A true MX2016007032A (es) | 2016-10-28 |
Family
ID=50190657
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2016007032A MX2016007032A (es) | 2013-12-06 | 2014-12-08 | Novedoso sistema de alteracion del genoma para microorganismos. |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US20160369300A1 (es) |
| EP (1) | EP3077521B1 (es) |
| JP (1) | JP2016538865A (es) |
| AU (1) | AU2014357808A1 (es) |
| BR (1) | BR112016012686A2 (es) |
| ES (1) | ES2694349T3 (es) |
| MX (1) | MX2016007032A (es) |
| NL (1) | NL2011912C2 (es) |
| PL (1) | PL3077521T3 (es) |
| RU (1) | RU2694316C1 (es) |
| WO (1) | WO2015084178A1 (es) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9879270B2 (en) * | 2014-08-15 | 2018-01-30 | Wisconsin Alumni Research Foundation | Constructs and methods for genome editing and genetic engineering of fungi and protists |
| CN107550996A (zh) * | 2017-10-25 | 2018-01-09 | 南京多宝生物科技有限公司 | 一种抑制胃酸分泌的枸橼酸铋钾胶囊及应用 |
| CN108504682A (zh) * | 2018-04-09 | 2018-09-07 | 西南大学 | 一种Cre/loxP基因删除系统及其应用 |
| CN112921049B (zh) * | 2021-02-06 | 2024-01-23 | 石河子大学 | 一种用于生产香草醛的基因片段、酿酒酵母工程菌及其构建方法 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5612205A (en) * | 1990-08-29 | 1997-03-18 | Genpharm International, Incorporated | Homologous recombination in mammalian cells |
| US6436643B1 (en) * | 1997-12-22 | 2002-08-20 | Unilever Patent Holdings Bv | Process for site-directed integration of multiple copies of a gene in a mould |
| BRPI0808704B1 (pt) * | 2007-03-02 | 2022-01-18 | Dupont Nutrition Biosciences Aps | Método para gerar uma cultura inicial compreendendo pelo menos duas cepas variantes resistentes a bacteriófagos, cultura iniciadora e método de fermentação |
| EP2126100B1 (en) * | 2007-03-21 | 2012-11-14 | DSM IP Assets B.V. | Improved method for homologous recombination |
| CN102421897B (zh) * | 2009-03-06 | 2015-12-16 | 合成基因组股份有限公司 | 用于克隆和操作基因组的方法 |
| US7919605B1 (en) * | 2010-08-30 | 2011-04-05 | Amyris, Inc. | Nucleic acids, compositions and methods for the excision of target nucleic acids |
-
2013
- 2013-12-06 NL NL2011912A patent/NL2011912C2/en not_active IP Right Cessation
-
2014
- 2014-12-08 JP JP2016535640A patent/JP2016538865A/ja active Pending
- 2014-12-08 WO PCT/NL2014/050839 patent/WO2015084178A1/en active Application Filing
- 2014-12-08 EP EP14815085.7A patent/EP3077521B1/en active Active
- 2014-12-08 MX MX2016007032A patent/MX2016007032A/es active IP Right Grant
- 2014-12-08 ES ES14815085.7T patent/ES2694349T3/es active Active
- 2014-12-08 RU RU2016126725A patent/RU2694316C1/ru active
- 2014-12-08 PL PL14815085T patent/PL3077521T3/pl unknown
- 2014-12-08 BR BR112016012686A patent/BR112016012686A2/pt not_active Application Discontinuation
- 2014-12-08 AU AU2014357808A patent/AU2014357808A1/en not_active Abandoned
- 2014-12-08 US US15/101,797 patent/US20160369300A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| AU2014357808A1 (en) | 2016-06-16 |
| PL3077521T3 (pl) | 2019-02-28 |
| RU2694316C1 (ru) | 2019-07-11 |
| ES2694349T3 (es) | 2018-12-20 |
| EP3077521B1 (en) | 2018-08-08 |
| JP2016538865A (ja) | 2016-12-15 |
| BR112016012686A2 (pt) | 2017-08-08 |
| EP3077521A1 (en) | 2016-10-12 |
| WO2015084178A1 (en) | 2015-06-11 |
| US20160369300A1 (en) | 2016-12-22 |
| NL2011912C2 (en) | 2015-06-09 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant or registration |