MX2015006376A - Casete de expresion optimizado para expresar un polipeptido con un alto rendimiento. - Google Patents
Casete de expresion optimizado para expresar un polipeptido con un alto rendimiento.Info
- Publication number
- MX2015006376A MX2015006376A MX2015006376A MX2015006376A MX2015006376A MX 2015006376 A MX2015006376 A MX 2015006376A MX 2015006376 A MX2015006376 A MX 2015006376A MX 2015006376 A MX2015006376 A MX 2015006376A MX 2015006376 A MX2015006376 A MX 2015006376A
- Authority
- MX
- Mexico
- Prior art keywords
- polypeptide
- expressing
- high yield
- expression cassette
- interest
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/30—Vector systems having a special element relevant for transcription being an enhancer not forming part of the promoter region
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
La presente invención se basa en el descubrimiento de que la combinación de una secuencia de polinucleótidos de 5'UTR específica (ver SEQ ID NO 1) y la secuencia líder secretora de hCD33 en un casete de expresión para expresar un polinucleótido de interés da como resultado un nivel de expresión sorprendentemente mejor del polipéptido de interés comparándose con los casetes de expresión de la técnica anterior. Basándose en este descubrimiento, la presente invención, entre otras cosas, proporciona novedosos casetes de expresión, vectores de expresión y métodos para producir un polipéptido de interés con un alto rendimiento.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261728459P | 2012-11-20 | 2012-11-20 | |
| PCT/EP2013/074114 WO2014079819A1 (en) | 2012-11-20 | 2013-11-18 | Optimized expression cassette for expressing a polypeptide with high yield |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2015006376A true MX2015006376A (es) | 2015-10-05 |
| MX359950B MX359950B (es) | 2018-10-17 |
Family
ID=49585414
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2015006376A MX359950B (es) | 2012-11-20 | 2013-11-18 | Casete de expresión optimizado para expresar un polipéptido con un alto rendimiento. |
Country Status (18)
| Country | Link |
|---|---|
| US (2) | US9663797B2 (es) |
| EP (1) | EP2922962B1 (es) |
| JP (1) | JP6388592B2 (es) |
| KR (1) | KR102286389B1 (es) |
| CN (2) | CN113981002A (es) |
| AU (1) | AU2013349844B2 (es) |
| BR (1) | BR112015011061B1 (es) |
| CA (1) | CA2891388C (es) |
| DK (1) | DK2922962T3 (es) |
| ES (1) | ES2620325T3 (es) |
| IL (1) | IL238703B (es) |
| LT (1) | LT2922962T (es) |
| MX (1) | MX359950B (es) |
| PL (1) | PL2922962T3 (es) |
| PT (1) | PT2922962T (es) |
| RU (1) | RU2682884C2 (es) |
| SG (1) | SG11201503326PA (es) |
| WO (1) | WO2014079819A1 (es) |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3431140A1 (de) | 1984-08-24 | 1986-03-06 | Behringwerke Ag, 3550 Marburg | Enhancer fuer eukaryotische expressionssysteme |
| GB8717430D0 (en) | 1987-07-23 | 1987-08-26 | Celltech Ltd | Recombinant dna product |
| ATE452207T1 (de) | 1992-08-21 | 2010-01-15 | Univ Bruxelles | Immunoglobuline ohne leichte ketten |
| EP1716233B1 (en) | 2004-01-30 | 2009-08-26 | Maxygen Holdings Ltd. | Regulated stop codon readthrough |
| JP4722048B2 (ja) | 2004-07-27 | 2011-07-13 | 独立行政法人理化学研究所 | Ｎ−デアセチラーゼ／ｎ−スルホトランスフェラーゼ２を発現するベクター |
| WO2007131774A1 (en) | 2006-05-17 | 2007-11-22 | F. Hoffmann-La Roche Ag | Polypeptide producing cells |
| SG176501A1 (en) * | 2006-08-23 | 2011-12-29 | Selexis Sa | Matrix attachment regions (mars) for increasing transcription and uses thereof |
| CN101679497A (zh) | 2007-03-26 | 2010-03-24 | 津莫吉尼蒂克斯公司 | 可溶性il-17ra/rc融合蛋白以及相关方法 |
| JP5701061B2 (ja) * | 2007-12-21 | 2015-04-15 | ノバルティス アーゲー | 哺乳類発現ベクター |
| EP2227549B1 (en) | 2007-12-21 | 2015-06-17 | Novartis AG | Selection system for eukaryotic cell culture based on a membrane-bound folate receptor gene |
| CA2726862A1 (en) * | 2008-06-30 | 2010-01-21 | Atgcell Inc. | Mammalian cell expression vectors and utilization |
| ES2413379T3 (es) | 2008-08-28 | 2013-07-16 | Novartis Ag | Presentación en la superficie celular de isoformas polipeptídicas mediante ultralectura de codón de terminación |
| BRPI1009752B1 (pt) | 2009-02-27 | 2020-03-10 | Novartis Ag | Vetor de expressão ou uma combinação de pelo menos dois vetores de expressão e métodos para sua produção e sua seleção, bem como processo para produzir um produto de interesse e uso de um meio de cultura seletivo |
-
2013
- 2013-11-18 RU RU2015124041A patent/RU2682884C2/ru active
- 2013-11-18 LT LTEP13792030.2T patent/LT2922962T/lt unknown
- 2013-11-18 KR KR1020157016125A patent/KR102286389B1/ko active IP Right Grant
- 2013-11-18 PT PT137920302T patent/PT2922962T/pt unknown
- 2013-11-18 US US14/443,863 patent/US9663797B2/en active Active
- 2013-11-18 CA CA2891388A patent/CA2891388C/en active Active
- 2013-11-18 CN CN202111282575.3A patent/CN113981002A/zh active Pending
- 2013-11-18 BR BR112015011061-4A patent/BR112015011061B1/pt active IP Right Grant
- 2013-11-18 PL PL13792030T patent/PL2922962T3/pl unknown
- 2013-11-18 WO PCT/EP2013/074114 patent/WO2014079819A1/en active Application Filing
- 2013-11-18 CN CN201380060589.8A patent/CN104968791B/zh active Active
- 2013-11-18 JP JP2015543403A patent/JP6388592B2/ja active Active
- 2013-11-18 EP EP13792030.2A patent/EP2922962B1/en active Active
- 2013-11-18 SG SG11201503326PA patent/SG11201503326PA/en unknown
- 2013-11-18 ES ES13792030.2T patent/ES2620325T3/es active Active
- 2013-11-18 MX MX2015006376A patent/MX359950B/es active IP Right Grant
- 2013-11-18 DK DK13792030.2T patent/DK2922962T3/en active
- 2013-11-18 AU AU2013349844A patent/AU2013349844B2/en active Active
-
2015
- 2015-05-07 IL IL238703A patent/IL238703B/en active IP Right Grant
-
2017
- 2017-04-21 US US15/493,837 patent/US11230718B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| BR112015011061A2 (pt) | 2017-08-22 |
| US20170356006A1 (en) | 2017-12-14 |
| PT2922962T (pt) | 2017-03-29 |
| RU2015124041A (ru) | 2017-01-10 |
| CN104968791B (zh) | 2021-11-19 |
| LT2922962T (lt) | 2017-04-10 |
| MX359950B (es) | 2018-10-17 |
| RU2682884C2 (ru) | 2019-03-22 |
| US9663797B2 (en) | 2017-05-30 |
| EP2922962B1 (en) | 2016-12-21 |
| CN113981002A (zh) | 2022-01-28 |
| AU2013349844B2 (en) | 2017-03-02 |
| US11230718B2 (en) | 2022-01-25 |
| AU2013349844A1 (en) | 2015-05-21 |
| CA2891388C (en) | 2021-09-14 |
| IL238703A0 (en) | 2015-06-30 |
| PL2922962T3 (pl) | 2017-06-30 |
| ES2620325T3 (es) | 2017-06-28 |
| JP6388592B2 (ja) | 2018-09-12 |
| US20160186206A1 (en) | 2016-06-30 |
| KR20150085537A (ko) | 2015-07-23 |
| EP2922962A1 (en) | 2015-09-30 |
| SG11201503326PA (en) | 2015-06-29 |
| CA2891388A1 (en) | 2014-05-30 |
| KR102286389B1 (ko) | 2021-08-05 |
| WO2014079819A1 (en) | 2014-05-30 |
| IL238703B (en) | 2019-02-28 |
| JP2015535427A (ja) | 2015-12-14 |
| BR112015011061A8 (pt) | 2020-03-17 |
| CN104968791A (zh) | 2015-10-07 |
| DK2922962T3 (en) | 2017-03-27 |
| BR112015011061B1 (pt) | 2022-09-27 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant or registration |