MX2012007712A - Expresion de hexosa cinasa en celulas huesped recombinantes. - Google Patents

Expresion de hexosa cinasa en celulas huesped recombinantes.

Info

Publication number
MX2012007712A
MX2012007712A MX2012007712A MX2012007712A MX2012007712A MX 2012007712 A MX2012007712 A MX 2012007712A MX 2012007712 A MX2012007712 A MX 2012007712A MX 2012007712 A MX2012007712 A MX 2012007712A MX 2012007712 A MX2012007712 A MX 2012007712A
Authority
MX
Mexico
Prior art keywords
recombinant host
methods
host cells
polypeptide
polynucleotide encoding
Prior art date
Application number
MX2012007712A
Other languages
English (en)
Inventor
Larry Cameron Anthony
Arthur Leo Kruckeberg
Brian James Paul
Original Assignee
Butamax Tm Advanced Biofuels
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Butamax Tm Advanced Biofuels filed Critical Butamax Tm Advanced Biofuels
Publication of MX2012007712A publication Critical patent/MX2012007712A/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16—Butanols
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La invención se refiere a una célula huésped recombinante que tiene (a) una modificación en un polinucleótido endógeno que codifica un polipéptido que tiene actividad hexocinasa de doble función; (b) un polinucleótido heterólogo que codifica un polipéptido que tiene una actividad hexosa cinasa; y opcionalmente (c) una modificación en un polinucleótido endógeno que codifica un polipéptido que tiene actividad de la piruvato decarboxilasa. Adicionalmente, la invención se refiere a métodos para preparar y usar tales células huéspedes recombinantes que incluyen, por ejemplo, métodos para aumentar el consumo de glucosa, métodos para aumentar el balanceo de redox, y/o métodos para aumentar la producción de un producto de una ruta que usa piruvato.
MX2012007712A 2009-12-29 2010-12-29 Expresion de hexosa cinasa en celulas huesped recombinantes. MX2012007712A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US29063909P 2009-12-29 2009-12-29
PCT/US2010/062397 WO2011082248A1 (en) 2009-12-29 2010-12-29 Expression of hexose kinase in recombinant host cells

Publications (1)

Publication Number Publication Date
MX2012007712A true MX2012007712A (es) 2012-08-15

Family

ID=43663717

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2012007712A MX2012007712A (es) 2009-12-29 2010-12-29 Expresion de hexosa cinasa en celulas huesped recombinantes.

Country Status (12)

Country Link
US (2) US8637289B2 (es)
EP (1) EP2519629A1 (es)
JP (1) JP2013515507A (es)
KR (1) KR20120099509A (es)
CN (1) CN102741401A (es)
AU (1) AU2010339511A1 (es)
BR (1) BR112012016043A2 (es)
CA (1) CA2785660A1 (es)
MX (1) MX2012007712A (es)
NZ (1) NZ600585A (es)
WO (1) WO2011082248A1 (es)
ZA (1) ZA201204383B (es)

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CA2784903A1 (en) 2009-12-29 2011-07-28 Butamax(Tm) Advanced Biofuels Llc Alcohol dehydrogenases (adh) useful for fermentive production of lower alkyl alcohols
NZ601241A (en) 2010-02-17 2014-08-29 Butamax Tm Advanced Biofuels Improving activity of fe-s cluster requiring proteins
WO2011159967A1 (en) 2010-06-18 2011-12-22 Butamax(Tm) Advanced Biofuels Llc Extraction solvents derived from oil for alcohol removal in extractive fermentation
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US9273330B2 (en) 2012-10-03 2016-03-01 Butamax Advanced Biofuels Llc Butanol tolerance in microorganisms
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WO2014144210A2 (en) 2013-03-15 2014-09-18 Butamax Advanced Biofuels Llc Competitive growth and/or production advantage for butanologen microorganism
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Also Published As

Publication number Publication date
KR20120099509A (ko) 2012-09-10
US20120015416A1 (en) 2012-01-19
CN102741401A (zh) 2012-10-17
EP2519629A1 (en) 2012-11-07
CA2785660A1 (en) 2011-07-07
US20140141479A1 (en) 2014-05-22
AU2010339511A1 (en) 2012-07-05
ZA201204383B (en) 2014-03-26
WO2011082248A1 (en) 2011-07-07
NZ600585A (en) 2014-07-25
JP2013515507A (ja) 2013-05-09
BR112012016043A2 (pt) 2015-11-24
US8637289B2 (en) 2014-01-28

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