MX2011007693A - Metodos para amplificar acidos nucleicos del virus de hepatitis c. - Google Patents
Metodos para amplificar acidos nucleicos del virus de hepatitis c.Info
- Publication number
- MX2011007693A MX2011007693A MX2011007693A MX2011007693A MX2011007693A MX 2011007693 A MX2011007693 A MX 2011007693A MX 2011007693 A MX2011007693 A MX 2011007693A MX 2011007693 A MX2011007693 A MX 2011007693A MX 2011007693 A MX2011007693 A MX 2011007693A
- Authority
- MX
- Mexico
- Prior art keywords
- seq
- nucleotide sequence
- nucleotides
- set forth
- optionally
- Prior art date
Links
- 102000039446 nucleic acids Human genes 0.000 title abstract 2
- 108020004707 nucleic acids Proteins 0.000 title abstract 2
- 241000711549 Hepacivirus C Species 0.000 title 1
- 150000007523 nucleic acids Chemical class 0.000 title 1
- 239000002773 nucleotide Substances 0.000 abstract 18
- 125000003729 nucleotide group Chemical group 0.000 abstract 18
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 abstract 1
- -1 HCV nucleic acid Chemical class 0.000 abstract 1
- 230000000295 complement effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/706—Specific hybridization probes for hepatitis
- C12Q1/707—Specific hybridization probes for hepatitis non-A, non-B Hepatitis, excluding hepatitis D
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2531/00—Reactions of nucleic acids characterised by
- C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
- C12Q2531/113—PCR
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Communicable Diseases (AREA)
- Pathology (AREA)
- Virology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Un método de amplificación de un ácido nucleico de HCV en una muestra infectada con HCV comprende amplificar un segmento de plantilla de ADN complementario a un genoma de hacv de ARN de la muestra por una PCR de dos etapas, en donde un PCR de primera etapa emplea un primer cavador externo y un segundo cebador externo, y un PCR de segunda etapa emplea un primer cebador interno y un segundo cebador interno. La secuencia de nucleótidos del primer cebador externo comprende una secuencia de nucleótidos como se establece en SEQ ID NO: 2; o SEQ ID NO: 9, donde opcionalmente 1, 2 o 3 nucleótidos son nucleótidos diferentes a aquellos de SEQ ID NO: 9. La secuencia de nucleótidos del segundo cebador externo comprende una secuencia de nucleótidos como se establece en SEQ ID NO: 3 o 4; o una secuencia de nucleótidos establecida en SEQ ID NO: 10 o 11, donde opcionalmente 1, 2 o 3 nucleótidos son nucleótidos diferentes a aquellos d SEQ ID NO: 10 y 11. La secuencia de nucleótidos del primer cebador interno comprende una secuencia de nucleótidos como se establece en SEQ ID NO: 5; o SEQ ID NO: 12, donde opcionalmente 1, 2 o 3 nucleótidos son nucleótidos diferentes a aquellos de SEQ ID NO: 12.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US14608309P | 2009-01-21 | 2009-01-21 | |
| PCT/US2010/021589 WO2010090857A2 (en) | 2009-01-21 | 2010-01-21 | Methods for amplifying hepatitis c virus nucleic acids |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2011007693A true MX2011007693A (es) | 2011-09-27 |
Family
ID=42334055
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2011007693A MX2011007693A (es) | 2009-01-21 | 2010-01-21 | Metodos para amplificar acidos nucleicos del virus de hepatitis c. |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US8679740B2 (es) |
| EP (1) | EP2389452A2 (es) |
| JP (1) | JP2012515534A (es) |
| KR (1) | KR20120101278A (es) |
| CN (1) | CN102341506A (es) |
| AU (1) | AU2010210892A1 (es) |
| CA (1) | CA2749969A1 (es) |
| IL (1) | IL214195A0 (es) |
| MX (1) | MX2011007693A (es) |
| NZ (1) | NZ594155A (es) |
| RU (1) | RU2011134857A (es) |
| WO (1) | WO2010090857A2 (es) |
| ZA (1) | ZA201105349B (es) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA3170924A1 (en) | 2007-10-02 | 2009-04-09 | Labrador Diagnostics Llc | Modular point-of-care devices and uses thereof |
| CN106323875B (zh) | 2011-01-21 | 2019-07-09 | 西拉诺斯知识产权有限责任公司 | 样品使用最大化的系统和方法 |
| US9619627B2 (en) | 2011-09-25 | 2017-04-11 | Theranos, Inc. | Systems and methods for collecting and transmitting assay results |
| US9664702B2 (en) | 2011-09-25 | 2017-05-30 | Theranos, Inc. | Fluid handling apparatus and configurations |
| US8475739B2 (en) | 2011-09-25 | 2013-07-02 | Theranos, Inc. | Systems and methods for fluid handling |
| US8840838B2 (en) | 2011-09-25 | 2014-09-23 | Theranos, Inc. | Centrifuge configurations |
| US9632102B2 (en) | 2011-09-25 | 2017-04-25 | Theranos, Inc. | Systems and methods for multi-purpose analysis |
| US9268915B2 (en) | 2011-09-25 | 2016-02-23 | Theranos, Inc. | Systems and methods for diagnosis or treatment |
| US20140170735A1 (en) | 2011-09-25 | 2014-06-19 | Elizabeth A. Holmes | Systems and methods for multi-analysis |
| US10012664B2 (en) | 2011-09-25 | 2018-07-03 | Theranos Ip Company, Llc | Systems and methods for fluid and component handling |
| US9810704B2 (en) | 2013-02-18 | 2017-11-07 | Theranos, Inc. | Systems and methods for multi-analysis |
| US9250229B2 (en) | 2011-09-25 | 2016-02-02 | Theranos, Inc. | Systems and methods for multi-analysis |
| JP2016532876A (ja) | 2013-09-06 | 2016-10-20 | セラノス， インコーポレイテッド | 拭い取りを受け取るための機器、システム、方法及びキット |
| EP3674422A3 (en) | 2013-09-06 | 2020-09-16 | Labrador Diagnostics LLC | Systems and methods for detecting infectious diseases |
| CN106222306A (zh) * | 2016-08-30 | 2016-12-14 | 合肥金域医学检验所有限公司 | 一种hcv病毒ns5a蛋白编码序列突变检测试剂盒 |
| GB201819726D0 (en) * | 2018-12-03 | 2019-01-16 | Diagnostics For The Real World Ltd | HCV detection |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4458066A (en) | 1980-02-29 | 1984-07-03 | University Patents, Inc. | Process for preparing polynucleotides |
| US4973679A (en) | 1981-03-27 | 1990-11-27 | University Patents, Inc. | Process for oligonucleo tide synthesis using phosphormidite intermediates |
| US4415732A (en) | 1981-03-27 | 1983-11-15 | University Patents, Inc. | Phosphoramidite compounds and processes |
| DE3329892A1 (de) | 1983-08-18 | 1985-03-07 | Köster, Hubert, Prof. Dr., 2000 Hamburg | Verfahren zur herstellung von oligonucleotiden |
| FR2596761B1 (fr) | 1986-04-08 | 1988-05-20 | Commissariat Energie Atomique | Derives de nucleosides et leur utilisation pour la synthese d'oligonucleotides |
| US5310652A (en) | 1986-08-22 | 1994-05-10 | Hoffman-La Roche Inc. | Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription |
| US5322770A (en) | 1989-12-22 | 1994-06-21 | Hoffman-Laroche Inc. | Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription |
| PT787807E (pt) | 1991-08-27 | 2003-08-29 | Hoffmann La Roche | Iniciadores e sondas para a deteccao da hepatite c |
| EP0532258A2 (en) * | 1991-09-09 | 1993-03-17 | Immuno Japan Inc. | Oligonucleotides and determination system of HCV genotypes |
| JPH0775585A (ja) * | 1993-06-14 | 1995-03-20 | Immuno Japan:Kk | Ｃ型肝炎ウイルス関連オリゴヌクレオチドならびにｃ型肝炎 ウイルス遺伝子型判定方法 |
| ZA966663B (en) | 1995-08-17 | 1998-02-06 | Genentech Inc | Traf Inhibitors. |
| US6153421A (en) * | 1997-07-18 | 2000-11-28 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Cloned genomes of infectious hepatitis C viruses and uses thereof |
| US6790952B2 (en) * | 2001-02-13 | 2004-09-14 | Cincinnati Children's Hospital Research Foundation | Quantitative epstein barr virus PCR rapid assay |
| JP3751867B2 (ja) * | 2001-09-18 | 2006-03-01 | 株式会社東芝 | インターフェロンを投与されるべき個体においてインターフェロン療法の有効性を予測する方法、その方法をコンピュータにより実行させるためのプログラム、インターフェロン感受性に関連する多型部位の遺伝子型を検出するための核酸プローブ、およびその核酸プローブを具備する塩基配列検出用チップ |
| US20050250093A1 (en) * | 2002-04-03 | 2005-11-10 | Gates Adam T | Hepatitis c virus sub-genomic replicons |
| US20040137539A1 (en) * | 2003-01-10 | 2004-07-15 | Bradford Sherry A. | Cancer comprehensive method for identifying cancer protein patterns and determination of cancer treatment strategies |
| BRPI0407587A (pt) | 2003-02-18 | 2006-02-14 | Pfizer | inibidores do vìrus da hepatite c, composições e tratamentos que os utilizam |
| US20050282179A1 (en) | 2003-12-05 | 2005-12-22 | Applera Corporation | Biomarkers for interferon-alpha response in hepatitis C virus infected patients |
| WO2006085407A1 (ja) * | 2005-02-09 | 2006-08-17 | Nihon University | Hcv量に関連する遺伝子のスクリーニング方法 |
| US20100173298A1 (en) * | 2007-04-27 | 2010-07-08 | Kenichi Mori | Hcv gene |
| JP4213759B2 (ja) * | 2007-08-10 | 2009-01-21 | 株式会社東芝 | 標的核酸の遺伝子型、および変異の判定方法、不溶性支持体に核酸断片を固定化する方法、所望の核酸を精製し、かつ増幅するための方法、並びに遺伝子型アッセイキット |
-
2010
- 2010-01-21 RU RU2011134857/10A patent/RU2011134857A/ru not_active Application Discontinuation
- 2010-01-21 JP JP2011546450A patent/JP2012515534A/ja active Pending
- 2010-01-21 NZ NZ594155A patent/NZ594155A/xx not_active IP Right Cessation
- 2010-01-21 KR KR1020117019326A patent/KR20120101278A/ko not_active Withdrawn
- 2010-01-21 CN CN2010800098527A patent/CN102341506A/zh active Pending
- 2010-01-21 AU AU2010210892A patent/AU2010210892A1/en not_active Abandoned
- 2010-01-21 WO PCT/US2010/021589 patent/WO2010090857A2/en not_active Ceased
- 2010-01-21 EP EP10703544A patent/EP2389452A2/en not_active Ceased
- 2010-01-21 CA CA2749969A patent/CA2749969A1/en not_active Abandoned
- 2010-01-21 MX MX2011007693A patent/MX2011007693A/es not_active Application Discontinuation
-
2011
- 2011-07-19 IL IL214195A patent/IL214195A0/en unknown
- 2011-07-20 US US13/186,985 patent/US8679740B2/en not_active Expired - Fee Related
- 2011-07-20 ZA ZA2011/05349A patent/ZA201105349B/en unknown
-
2014
- 2014-01-28 US US14/165,757 patent/US20140199684A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP2389452A2 (en) | 2011-11-30 |
| CN102341506A (zh) | 2012-02-01 |
| RU2011134857A (ru) | 2013-02-27 |
| KR20120101278A (ko) | 2012-09-13 |
| US20120129155A1 (en) | 2012-05-24 |
| AU2010210892A1 (en) | 2011-08-11 |
| US20140199684A1 (en) | 2014-07-17 |
| NZ594155A (en) | 2013-02-22 |
| CA2749969A1 (en) | 2010-08-12 |
| IL214195A0 (en) | 2011-08-31 |
| WO2010090857A2 (en) | 2010-08-12 |
| WO2010090857A8 (en) | 2012-06-28 |
| US8679740B2 (en) | 2014-03-25 |
| WO2010090857A3 (en) | 2010-11-25 |
| ZA201105349B (en) | 2013-10-30 |
| JP2012515534A (ja) | 2012-07-12 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FA | Abandonment or withdrawal |