MX2011007693A - Metodos para amplificar acidos nucleicos del virus de hepatitis c. - Google Patents

Metodos para amplificar acidos nucleicos del virus de hepatitis c.

Info

Publication number
MX2011007693A
MX2011007693A MX2011007693A MX2011007693A MX2011007693A MX 2011007693 A MX2011007693 A MX 2011007693A MX 2011007693 A MX2011007693 A MX 2011007693A MX 2011007693 A MX2011007693 A MX 2011007693A MX 2011007693 A MX2011007693 A MX 2011007693A
Authority
MX
Mexico
Prior art keywords
seq
nucleotide sequence
nucleotides
set forth
optionally
Prior art date
Application number
MX2011007693A
Other languages
English (en)
Inventor
Chao Lin
Yi Zhou
Ann D Kwong
James Daniel Frantz
Douglas J Bartels
Benjamin Shames
Sheila Seepersaud
Judith A Lippke
Tara L Kieffer
Eileen Z Zhang
James C Sullivan
Original Assignee
Vertex Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vertex Pharma filed Critical Vertex Pharma
Publication of MX2011007693A publication Critical patent/MX2011007693A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis
    • C12Q1/707—Specific hybridization probes for hepatitis non-A, non-B Hepatitis, excluding hepatitis D
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113—PCR

Abstract

Un método de amplificación de un ácido nucleico de HCV en una muestra infectada con HCV comprende amplificar un segmento de plantilla de ADN complementario a un genoma de hacv de ARN de la muestra por una PCR de dos etapas, en donde un PCR de primera etapa emplea un primer cavador externo y un segundo cebador externo, y un PCR de segunda etapa emplea un primer cebador interno y un segundo cebador interno. La secuencia de nucleótidos del primer cebador externo comprende una secuencia de nucleótidos como se establece en SEQ ID NO: 2; o SEQ ID NO: 9, donde opcionalmente 1, 2 o 3 nucleótidos son nucleótidos diferentes a aquellos de SEQ ID NO: 9. La secuencia de nucleótidos del segundo cebador externo comprende una secuencia de nucleótidos como se establece en SEQ ID NO: 3 o 4; o una secuencia de nucleótidos establecida en SEQ ID NO: 10 o 11, donde opcionalmente 1, 2 o 3 nucleótidos son nucleótidos diferentes a aquellos d SEQ ID NO: 10 y 11. La secuencia de nucleótidos del primer cebador interno comprende una secuencia de nucleótidos como se establece en SEQ ID NO: 5; o SEQ ID NO: 12, donde opcionalmente 1, 2 o 3 nucleótidos son nucleótidos diferentes a aquellos de SEQ ID NO: 12.
MX2011007693A 2009-01-21 2010-01-21 Metodos para amplificar acidos nucleicos del virus de hepatitis c. MX2011007693A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14608309P 2009-01-21 2009-01-21
PCT/US2010/021589 WO2010090857A2 (en) 2009-01-21 2010-01-21 Methods for amplifying hepatitis c virus nucleic acids

Publications (1)

Publication Number Publication Date
MX2011007693A true MX2011007693A (es) 2011-09-27

Family

ID=42334055

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2011007693A MX2011007693A (es) 2009-01-21 2010-01-21 Metodos para amplificar acidos nucleicos del virus de hepatitis c.

Country Status (13)

Country Link
US (2) US8679740B2 (es)
EP (1) EP2389452A2 (es)
JP (1) JP2012515534A (es)
KR (1) KR20120101278A (es)
CN (1) CN102341506A (es)
AU (1) AU2010210892A1 (es)
CA (1) CA2749969A1 (es)
IL (1) IL214195A0 (es)
MX (1) MX2011007693A (es)
NZ (1) NZ594155A (es)
RU (1) RU2011134857A (es)
WO (1) WO2010090857A2 (es)
ZA (1) ZA201105349B (es)

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TWI639703B (zh) 2011-01-21 2018-11-01 賽瑞諾斯有限公司 樣本使用最大化之系統及方法
US9632102B2 (en) 2011-09-25 2017-04-25 Theranos, Inc. Systems and methods for multi-purpose analysis
US9619627B2 (en) 2011-09-25 2017-04-11 Theranos, Inc. Systems and methods for collecting and transmitting assay results
US8475739B2 (en) 2011-09-25 2013-07-02 Theranos, Inc. Systems and methods for fluid handling
US9268915B2 (en) 2011-09-25 2016-02-23 Theranos, Inc. Systems and methods for diagnosis or treatment
US20140170735A1 (en) 2011-09-25 2014-06-19 Elizabeth A. Holmes Systems and methods for multi-analysis
US9664702B2 (en) 2011-09-25 2017-05-30 Theranos, Inc. Fluid handling apparatus and configurations
US8840838B2 (en) 2011-09-25 2014-09-23 Theranos, Inc. Centrifuge configurations
US9810704B2 (en) 2013-02-18 2017-11-07 Theranos, Inc. Systems and methods for multi-analysis
US9250229B2 (en) 2011-09-25 2016-02-02 Theranos, Inc. Systems and methods for multi-analysis
US10012664B2 (en) 2011-09-25 2018-07-03 Theranos Ip Company, Llc Systems and methods for fluid and component handling
WO2015035260A1 (en) 2013-09-06 2015-03-12 Theranos, Inc. Systems and methods for detecting infectious diseases
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Also Published As

Publication number Publication date
KR20120101278A (ko) 2012-09-13
ZA201105349B (en) 2013-10-30
CA2749969A1 (en) 2010-08-12
WO2010090857A2 (en) 2010-08-12
RU2011134857A (ru) 2013-02-27
NZ594155A (en) 2013-02-22
JP2012515534A (ja) 2012-07-12
WO2010090857A8 (en) 2012-06-28
US8679740B2 (en) 2014-03-25
US20140199684A1 (en) 2014-07-17
EP2389452A2 (en) 2011-11-30
WO2010090857A3 (en) 2010-11-25
US20120129155A1 (en) 2012-05-24
AU2010210892A1 (en) 2011-08-11
CN102341506A (zh) 2012-02-01
IL214195A0 (en) 2011-08-31

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