WO2012046981A3 - Real time pcr detection of single nucleotide polymorphisms - Google Patents

Real time pcr detection of single nucleotide polymorphisms Download PDF

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Publication number
WO2012046981A3
WO2012046981A3 PCT/KR2011/007294 KR2011007294W WO2012046981A3 WO 2012046981 A3 WO2012046981 A3 WO 2012046981A3 KR 2011007294 W KR2011007294 W KR 2011007294W WO 2012046981 A3 WO2012046981 A3 WO 2012046981A3
Authority
WO
WIPO (PCT)
Prior art keywords
snp
time pcr
rna
single nucleotide
real
Prior art date
Application number
PCT/KR2011/007294
Other languages
French (fr)
Other versions
WO2012046981A2 (en
Inventor
Jason Opdyke
John Harvey
Original Assignee
Samsung Techwin Co., Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US13/158,593 external-priority patent/US20120088246A1/en
Application filed by Samsung Techwin Co., Ltd. filed Critical Samsung Techwin Co., Ltd.
Priority to CN2011800488109A priority Critical patent/CN103154271A/en
Priority to EP11830866.7A priority patent/EP2625290A4/en
Priority to JP2013532717A priority patent/JP2013545442A/en
Publication of WO2012046981A2 publication Critical patent/WO2012046981A2/en
Publication of WO2012046981A3 publication Critical patent/WO2012046981A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/327—RNAse, e.g. RNAseH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2561/00—Nucleic acid detection characterised by assay method
    • C12Q2561/113—Real time assay

Abstract

Disclosed are methods and kits for the detection of a polymorphism during real-time PCR. Real-time PCR amplification of a target nucleic acid sequence is performed using PCR primer primers that anneal to sequences flanking a single nucleotide polymorphism (SNP) of interest. The real-time PCR reaction includes a labeled probe comprising a RNA sequence that is designed to anneal to DNA sequences at the location of the SNP. An RNA:DNA heteroduplex can then form between the SNP in the PCR fragment and the probe's RNA sequences that are complementary to the SNP. RNase H cleavage of the RNA sequence in the RNA:DNA heteroduplex results in increase in intensity of the signal generated from the label that is indicative of the presence of an SNP in the target nucleic acid.
PCT/KR2011/007294 2010-10-04 2011-10-04 Real time pcr detection of single nucleotide polymorphisms WO2012046981A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CN2011800488109A CN103154271A (en) 2010-10-04 2011-10-04 Real time pcr detection of single nucleotide polymorphisms
EP11830866.7A EP2625290A4 (en) 2010-10-04 2011-10-04 Real time pcr detection of single nucleotide polymorphisms
JP2013532717A JP2013545442A (en) 2010-10-04 2011-10-04 Real-time PCR detection of single nucleotide polymorphisms

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US38941210P 2010-10-04 2010-10-04
US61/389,412 2010-10-04
US39070110P 2010-10-07 2010-10-07
US61/390,701 2010-10-07
US13/158,593 2011-06-13
US13/158,593 US20120088246A1 (en) 2010-10-07 2011-06-13 Real time pcr detection of single nucleotide polymorphisms

Publications (2)

Publication Number Publication Date
WO2012046981A2 WO2012046981A2 (en) 2012-04-12
WO2012046981A3 true WO2012046981A3 (en) 2012-07-19

Family

ID=45928196

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2011/007294 WO2012046981A2 (en) 2010-10-04 2011-10-04 Real time pcr detection of single nucleotide polymorphisms

Country Status (5)

Country Link
EP (1) EP2625290A4 (en)
JP (1) JP2013545442A (en)
KR (1) KR20120046018A (en)
CN (1) CN103154271A (en)
WO (1) WO2012046981A2 (en)

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Publication number Priority date Publication date Assignee Title
CN111118114B (en) * 2013-11-26 2023-06-23 杭州联川基因诊断技术有限公司 Method for generating surface clusters
EP3082800B1 (en) 2013-12-20 2022-02-02 Biomed Valley Discoveries, Inc. Cancer treatment using combinations of erk and raf inhibitors
JP6727127B2 (en) 2013-12-20 2020-07-29 バイオメッド バレー ディスカバリーズ，インコーポレイティド Cancer treatment using a combination of PI3K/AKT signaling pathway inhibitors and ERK inhibitors
EP3082779B1 (en) 2013-12-20 2023-06-07 Biomed Valley Discoveries, Inc. Cancer treatments using combinations of type 2 mek and erk inhibitors
WO2015095840A1 (en) 2013-12-20 2015-06-25 Biomed Valley Discoveries, Inc. Cancer treatments using combinations of cdk and erk inhibitors
KR101598398B1 (en) 2014-01-08 2016-02-29 (주)제노텍 Mutant Detection Method by Real-Time Polymerase Chain Reaction using selective working of 5'-flap endonuclease activity
CN104911265A (en) * 2015-06-03 2015-09-16 辽宁省农业科学院蔬菜研究所 Dual-PCR method for detecting anti-disease genes Ty-3a and Tm-2<a> of lycopersicon esculentum simultaneously
WO2018026039A1 (en) * 2016-08-05 2018-02-08 주식회사 하임바이오텍 Kit and method for detecting single nucleotide polymorphism
US20190093155A1 (en) * 2017-05-25 2019-03-28 Roche Molecular Systems, Inc. Multiplex Nucleic Acid Amplification Assay
CN108588252A (en) * 2018-03-29 2018-09-28 杭州泰熙生物技术有限公司 A kind of parasite detection kit and its detection method
KR102275038B1 (en) * 2019-12-11 2021-07-08 (주) 제노텍 A PCR method for increasing the discrimination of allele and a PCR kit for increasing the discrimination of allele
CN113215163B (en) * 2021-05-12 2023-05-12 苏州海苗生物科技有限公司 Molecular lock for specifically amplifying target gene and application thereof
CN117089605A (en) * 2023-05-08 2023-11-21 中国科学院苏州生物医学工程技术研究所 FQ-RCA-based RNA isothermal real-time genotyping method

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020058255A1 (en) * 2000-09-15 2002-05-16 Thomas Thum PCR reaction mixture for fluorescence-based gene expression and gene mutation analyses
US20060121487A1 (en) * 2002-12-27 2006-06-08 David Alland Method for single nucleotide polymorphism detection
US20070048762A1 (en) * 2002-09-09 2007-03-01 Ghazala Hashmi Real-time monitoring of nucleic acid target-identifying signals
US20100009350A1 (en) * 2008-07-10 2010-01-14 National Tsing Hua University Method for quantitative analysis of transcripts with nucleotide polymorphism at specific site

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996021144A1 (en) * 1994-12-30 1996-07-11 Georgetown University Fluorometric assay for detecting nucleic acid cleavage

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020058255A1 (en) * 2000-09-15 2002-05-16 Thomas Thum PCR reaction mixture for fluorescence-based gene expression and gene mutation analyses
US20070048762A1 (en) * 2002-09-09 2007-03-01 Ghazala Hashmi Real-time monitoring of nucleic acid target-identifying signals
US20060121487A1 (en) * 2002-12-27 2006-06-08 David Alland Method for single nucleotide polymorphism detection
US20100009350A1 (en) * 2008-07-10 2010-01-14 National Tsing Hua University Method for quantitative analysis of transcripts with nucleotide polymorphism at specific site

Also Published As

Publication number Publication date
JP2013545442A (en) 2013-12-26
KR20120046018A (en) 2012-05-09
EP2625290A4 (en) 2014-04-02
EP2625290A2 (en) 2013-08-14
CN103154271A (en) 2013-06-12
WO2012046981A2 (en) 2012-04-12

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