MX2011007643A - Dispositivo microfluidico funcionalizado para inmunofluorescencia. - Google Patents

Dispositivo microfluidico funcionalizado para inmunofluorescencia.

Info

Publication number
MX2011007643A
MX2011007643A MX2011007643A MX2011007643A MX2011007643A MX 2011007643 A MX2011007643 A MX 2011007643A MX 2011007643 A MX2011007643 A MX 2011007643A MX 2011007643 A MX2011007643 A MX 2011007643A MX 2011007643 A MX2011007643 A MX 2011007643A
Authority
MX
Mexico
Prior art keywords
slide
cells
microfluidic device
functionalized
notch
Prior art date
Application number
MX2011007643A
Other languages
English (en)
Spanish (es)
Inventor
Roberta Carbone
Emanuele Barborini
Dario Bandiera
Original Assignee
Tethis S P A
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tethis S P A filed Critical Tethis S P A
Publication of MX2011007643A publication Critical patent/MX2011007643A/es

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0093—Microreactors, e.g. miniaturised or microfabricated reactors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5302—Apparatus specially adapted for immunological test procedures
    • G01N33/5304—Reaction vessels, e.g. agglutination plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0636—Integrated biosensor, microarrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0822—Slides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/72—Assays involving receptors, cell surface antigens or cell surface determinants for hormones
    • G01N2333/726—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54346—Nanoparticles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • G01N33/553—Metal or metal coated

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • General Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Nanotechnology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • Composite Materials (AREA)
  • Materials Engineering (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Inorganic Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
MX2011007643A 2009-01-26 2010-01-26 Dispositivo microfluidico funcionalizado para inmunofluorescencia. MX2011007643A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PCT/EP2009/000465 WO2010083852A1 (en) 2009-01-26 2009-01-26 Functionalized microfluidic device for immunofluorescence
PCT/EP2010/050820 WO2010084196A1 (en) 2009-01-26 2010-01-26 Functionalized microfluidic device for immunofluorescence

Publications (1)

Publication Number Publication Date
MX2011007643A true MX2011007643A (es) 2011-10-28

Family

ID=40566192

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2011007643A MX2011007643A (es) 2009-01-26 2010-01-26 Dispositivo microfluidico funcionalizado para inmunofluorescencia.

Country Status (15)

Country Link
US (1) US9364830B2 (zh)
EP (1) EP2291540B1 (zh)
JP (1) JP5719313B2 (zh)
CN (1) CN102308005B (zh)
BR (1) BRPI1007011A2 (zh)
CA (1) CA2750682C (zh)
DK (1) DK2291540T3 (zh)
ES (1) ES2446277T3 (zh)
HK (1) HK1154637A1 (zh)
HR (1) HRP20140042T1 (zh)
IL (1) IL214238A (zh)
MX (1) MX2011007643A (zh)
PL (1) PL2291540T3 (zh)
PT (1) PT2291540E (zh)
WO (2) WO2010083852A1 (zh)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9995688B2 (en) * 2009-09-21 2018-06-12 Arizona Board Of Regents Use of superhydrophobic surfaces for liquid agglutination assays
WO2012061818A1 (en) * 2010-11-05 2012-05-10 Life Technologies Corporation Flowcells and flowcell reaction chambers
JP6084370B2 (ja) * 2011-05-10 2017-02-22 国立大学法人 東京大学 組織化学用自動反応装置
DE112013001115T5 (de) * 2012-02-23 2014-11-06 National University Corporation Shimane University Mikrokanalchip
CN104777101A (zh) * 2015-04-24 2015-07-15 王丹凤 一种观察细胞的方法
IT201600099710A1 (it) * 2016-10-05 2018-04-05 Tethis S P A Portacampioni per analisi di spettrometria di massa in modalità maldi, produzione ed uso del portacampioni
IT201700085439A1 (it) * 2017-07-26 2019-01-26 Tethis S P A Metodo di immobilizzazione di campioni biologici a scopo analitico e diagnostico
KR101952443B1 (ko) * 2018-08-23 2019-02-26 주식회사 아스트로젠 신규한 마그네슘­세리네이트 화합물 및 이의 용도
CN109060688A (zh) * 2018-08-27 2018-12-21 南京采薇且歌信息科技有限公司 一种高精度纳米传感器及其应用
JP7347931B2 (ja) * 2018-12-26 2023-09-20 スリーエム イノベイティブ プロパティズ カンパニー マイクロ流体デバイス用フィルム、マイクロ流体デバイス及びその製造方法
JP7356241B2 (ja) * 2019-03-18 2023-10-04 フコク物産株式会社 マイクロ流体デバイス
TWI762811B (zh) * 2019-08-30 2022-05-01 國立臺灣科技大學 負載有多孔隙奈米顆粒結構之多孔隙基材及其負載製程
CN110553888B (zh) * 2019-09-16 2022-07-12 中国农业科学院特产研究所 一种卵母细胞免疫荧光染色后快速压片的方法
CN110951834A (zh) * 2019-12-10 2020-04-03 中国科学院深圳先进技术研究院 一种基于微流控芯片的细胞荧光原位杂交方法及其应用

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU1911795A (en) * 1994-02-09 1995-08-29 Abbott Laboratories Diagnostic flow cell device
US6613560B1 (en) * 1994-10-19 2003-09-02 Agilent Technologies, Inc. PCR microreactor for amplifying DNA using microquantities of sample fluid
WO1998055651A1 (en) * 1997-06-05 1998-12-10 University Of Massachusetts Method for determining rate of movement of a molecule in a living cell
AU1831700A (en) * 1998-12-01 2000-06-19 Syntrix Biochip, Inc. Methods and compositions for performing an array of chemical reactions on a support surface
IT1310029B1 (it) 1999-02-26 2002-02-05 Ist Naz Fisica Della Materia Vaporizzatore a microplasma pulsato.
JP3993372B2 (ja) * 2000-09-13 2007-10-17 独立行政法人理化学研究所 リアクタの製造方法
CA2424941A1 (en) * 2000-10-10 2002-04-18 Aviva Biosciences Corporation An integrated biochip system for sample preparation and analysis
US20040043479A1 (en) * 2000-12-11 2004-03-04 Briscoe Cynthia G. Multilayerd microfluidic devices for analyte reactions
FR2818287B1 (fr) * 2000-12-14 2003-01-17 Commissariat Energie Atomique Support solide pour l'immobilisation d'oligonucleotides
ES2372007T3 (es) 2001-02-20 2012-01-12 Vysis, Inc. Métodos y sondas para la detección del cáncer.
EP1542010A4 (en) * 2002-07-12 2007-11-21 Mitsubishi Chem Corp ANALYSIS CHIP, ANALYTICAL CHIP UNIT, ANALYSIS APPARATUS, ANALYSIS METHOD WITH THE APPARATUS AND METHOD FOR PRODUCING THE ANALYSIS CHIP
WO2004011672A1 (en) * 2002-07-26 2004-02-05 Ntera Limited Porous nanostructured film sensor
JP4519124B2 (ja) * 2003-01-30 2010-08-04 ユィロス・パテント・アクチボラグ 微小流動性デバイスの内部の壁
FR2872912B1 (fr) * 2004-07-09 2007-03-02 Centre Nat Rech Scient Cnrse Nouveau systeme microfluidique et procede de capture de cellules
EP1910835B1 (en) * 2005-07-21 2012-03-07 Tethis S.p.A. Support having nanostructured titanium dioxide film and uses thereof
JP2009519889A (ja) * 2005-12-19 2009-05-21 ナショナル・センター・フォア・サイエンティフィック・リサーチ・デモクリトス 修飾されたナノ構造チタニア材料及び製造方法
US7695956B2 (en) * 2006-01-12 2010-04-13 Biocept, Inc. Device for cell separation and analysis and method of using
WO2008031228A1 (en) * 2006-09-15 2008-03-20 The Governors Of The University Of Alberta Automated fish analysis, circulating microfluidic chip, and method for immobilizing cells to a microfluidic chip

Also Published As

Publication number Publication date
EP2291540A1 (en) 2011-03-09
DK2291540T3 (en) 2014-02-17
ES2446277T3 (es) 2014-03-06
JP5719313B2 (ja) 2015-05-13
CN102308005B (zh) 2015-07-15
IL214238A (en) 2017-11-30
AU2010207677A1 (en) 2011-09-08
IL214238A0 (en) 2011-09-27
US20110281267A1 (en) 2011-11-17
BRPI1007011A2 (pt) 2016-03-29
WO2010084196A1 (en) 2010-07-29
HRP20140042T1 (hr) 2014-02-28
PT2291540E (pt) 2014-03-03
US9364830B2 (en) 2016-06-14
EP2291540B1 (en) 2013-11-20
JP2012515908A (ja) 2012-07-12
HK1154637A1 (en) 2012-04-27
CA2750682C (en) 2016-10-25
WO2010083852A1 (en) 2010-07-29
PL2291540T3 (pl) 2014-05-30
AU2010207677A2 (en) 2011-09-08
CN102308005A (zh) 2012-01-04
CA2750682A1 (en) 2010-07-29

Similar Documents

Publication Publication Date Title
US9364830B2 (en) Functionalized microfluidic device for immunofluorescence
US8304026B2 (en) Microporous materials, methods of making, using, and articles thereof
JP5620444B2 (ja) 液滴中においてサンプルを処理するためのデバイスをリンスする装置、および当該デバイスをリンスする方法
JP4972636B2 (ja) 薄膜コーティングで被覆されたマイクロウェルアレイおよびそれを製造する方法
US7682688B2 (en) Microporous materials, methods, and articles for localizing and quantifying analytes
CN112368077B (zh) 流动池装置及其用途
CN107694649B (zh) 基于编码芯片的微阵列、其制备方法及应用
CN106460073B (zh) 多孔毛细管膜用于测定滚环扩增产物的量的应用
US20180264466A1 (en) Position-defined cell culture and characterization platform
AU2010207677B2 (en) Functionalized microfluidic device for immunofluorescence
Xing et al. Capture of cervical exfoliative cells on a glass slide coated by 3-glycidyloxypropyl trimethoxysilane and poly-L-lysine

Legal Events

Date Code Title Description
FG Grant or registration