MX166981B - METODO PARA TRASPLANTAR NUCLEO DE UN EMBRION DE BOVINO DONANTE A UN OOCITO RECEPTOR,"La presente invención se refiere a un método para transplantar un núcleo de un embrión bovino que comprende los siguientes pasos a) el aislamiento de un núcleo limitado por la membrana envolvente de una célula del embrión donante, b) la extracción de material cromosómico nuclear del oocito receptor para preparar un oocito receptor nucleado; c) la colocación del núcleo donado limitado por la membrana envolvente de manera que dicha membrana quede adyacente a la membrana del oocito receptor enucleado d) la fusión eléctrica de las membranas del núcleo limitado por su membrana envolvente y del occito receptor enucleado, fusionándose ambas membranas entre sí para formar una sola célula embrionario individual con un núcleo del embrión donante. - Google Patents

METODO PARA TRASPLANTAR NUCLEO DE UN EMBRION DE BOVINO DONANTE A UN OOCITO RECEPTOR,"La presente invención se refiere a un método para transplantar un núcleo de un embrión bovino que comprende los siguientes pasos a) el aislamiento de un núcleo limitado por la membrana envolvente de una célula del embrión donante, b) la extracción de material cromosómico nuclear del oocito receptor para preparar un oocito receptor nucleado; c) la colocación del núcleo donado limitado por la membrana envolvente de manera que dicha membrana quede adyacente a la membrana del oocito receptor enucleado d) la fusión eléctrica de las membranas del núcleo limitado por su membrana envolvente y del occito receptor enucleado, fusionándose ambas membranas entre sí para formar una sola célula embrionario individual con un núcleo del embrión donante.

Info

Publication number
MX166981B
MX166981B MX009953A MX995387A MX166981B MX 166981 B MX166981 B MX 166981B MX 009953 A MX009953 A MX 009953A MX 995387 A MX995387 A MX 995387A MX 166981 B MX166981 B MX 166981B
Authority
MX
Mexico
Prior art keywords
nucleus
membrane
limited
embryo
receptor
Prior art date
Application number
MX009953A
Other languages
English (en)
Inventor
James M Robl
Neal L First
Frank Barnes
Randall S Prather
Original Assignee
Grace W R & Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Grace W R & Co filed Critical Grace W R & Co
Publication of MX166981B publication Critical patent/MX166981B/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C12N15/877—Techniques for producing new mammalian cloned embryos
    • C12N15/8771—Bovine embryos

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
MX009953A 1986-12-31 1987-12-29 METODO PARA TRASPLANTAR NUCLEO DE UN EMBRION DE BOVINO DONANTE A UN OOCITO RECEPTOR,"La presente invención se refiere a un método para transplantar un núcleo de un embrión bovino que comprende los siguientes pasos a) el aislamiento de un núcleo limitado por la membrana envolvente de una célula del embrión donante, b) la extracción de material cromosómico nuclear del oocito receptor para preparar un oocito receptor nucleado; c) la colocación del núcleo donado limitado por la membrana envolvente de manera que dicha membrana quede adyacente a la membrana del oocito receptor enucleado d) la fusión eléctrica de las membranas del núcleo limitado por su membrana envolvente y del occito receptor enucleado, fusionándose ambas membranas entre sí para formar una sola célula embrionario individual con un núcleo del embrión donante. MX166981B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US94826986A 1986-12-31 1986-12-31
US07/113,791 US4994384A (en) 1986-12-31 1987-10-27 Multiplying bovine embryos

Publications (1)

Publication Number Publication Date
MX166981B true MX166981B (es) 1993-02-18

Family

ID=26811480

Family Applications (1)

Application Number Title Priority Date Filing Date
MX009953A MX166981B (es) 1986-12-31 1987-12-29 METODO PARA TRASPLANTAR NUCLEO DE UN EMBRION DE BOVINO DONANTE A UN OOCITO RECEPTOR,"La presente invención se refiere a un método para transplantar un núcleo de un embrión bovino que comprende los siguientes pasos a) el aislamiento de un núcleo limitado por la membrana envolvente de una célula del embrión donante, b) la extracción de material cromosómico nuclear del oocito receptor para preparar un oocito receptor nucleado; c) la colocación del núcleo donado limitado por la membrana envolvente de manera que dicha membrana quede adyacente a la membrana del oocito receptor enucleado d) la fusión eléctrica de las membranas del núcleo limitado por su membrana envolvente y del occito receptor enucleado, fusionándose ambas membranas entre sí para formar una sola célula embrionario individual con un núcleo del embrión donante.

Country Status (11)

Country Link
US (1) US4994384A (es)
JP (1) JP2882527B2 (es)
AU (1) AU623846B2 (es)
CA (1) CA1338931C (es)
CH (1) CH685765A5 (es)
DE (1) DE3744113C2 (es)
FR (1) FR2609045B1 (es)
GB (1) GB2199845A (es)
MX (1) MX166981B (es)
NL (1) NL8703088A (es)
NZ (1) NZ222740A (es)

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Also Published As

Publication number Publication date
NZ222740A (en) 1990-12-21
CH685765A5 (de) 1995-09-29
JP2882527B2 (ja) 1999-04-12
FR2609045A1 (fr) 1988-07-01
DE3744113C2 (de) 1998-04-09
AU623846B2 (en) 1992-05-28
CA1338931C (en) 1997-02-25
AU8318887A (en) 1988-07-07
GB2199845A (en) 1988-07-20
FR2609045B1 (fr) 1993-09-17
JPS63291523A (ja) 1988-11-29
GB8729856D0 (en) 1988-02-03
US4994384A (en) 1991-02-19
NL8703088A (nl) 1988-07-18
DE3744113A1 (de) 1988-07-14

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