MD653Z - Method for determination of drought-resistant genotypes of tomato - Google Patents
Method for determination of drought-resistant genotypes of tomatoInfo
- Publication number
- MD653Z MD653Z MDS20130036A MDS20130036A MD653Z MD 653 Z MD653 Z MD 653Z MD S20130036 A MDS20130036 A MD S20130036A MD S20130036 A MDS20130036 A MD S20130036A MD 653 Z MD653 Z MD 653Z
- Authority
- MD
- Moldova
- Prior art keywords
- pollen
- sample
- drought
- fractions
- control
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 11
- 235000007688 Lycopersicon esculentum Nutrition 0.000 title claims abstract description 8
- 240000003768 Solanum lycopersicum Species 0.000 title claims abstract 3
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 claims description 2
- 239000000600 sorbitol Substances 0.000 claims description 2
- 238000000926 separation method Methods 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 8
- 241000227653 Lycopersicon Species 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 4
- 241000722946 Acanthocybium solandri Species 0.000 description 3
- 241000405961 Scomberomorus regalis Species 0.000 description 3
- 241001263551 Solanum cheesmaniae Species 0.000 description 3
- 241000896499 Solanum habrochaites Species 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 230000003204 osmotic effect Effects 0.000 description 2
- 230000002269 spontaneous effect Effects 0.000 description 2
- 235000003363 Cornus mas Nutrition 0.000 description 1
- 240000006766 Cornus mas Species 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 210000005069 ears Anatomy 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/13—Abiotic stress
- Y02A40/132—Plants tolerant to drought
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Invenţia se referă la agricultură, în particular la metode de determinare a genotipurilor de tomate rezistente la secetă.Metoda, conform invenţiei, include colectarea florilor complet deschise de fiecare genotip, separarea polenului din antere, divizarea polenului în două fracţii, fracţia martor se amplasează pe o lamă-portobiect uscată, iar fracţia de probă se amplasează pe o lamă-portobiect într-o picătură de soluţie de sorbit de 85%, timp de 2 ore, apoi la microscop se determină dimensiunile grăuncioarelor de polen cu ajutorul micrometrului ocular în fracţiile martor şi de probă, după care se determină raportul dimensiunilor grăuncioarelor de polen ale fracţiilor de probă şi martor, exprimat în procente, totodată cu cât este mai mare acest raport, cu atât genotipul este mai rezistent la secetă.The invention relates to agriculture, in particular to methods for determining drought-resistant tomato genotypes. The method, according to the invention, includes the collection of flowers completely opened by each genotype, the separation of pollen from anthers, the division of pollen into two fractions, the control fraction being placed on a dry slide-slide, and the sample fraction is placed on a slide-slide in a drop of 85% sorbit solution, for 2 hours, and then the microscope determines the dimensions of the pollen grains using the ocular micrometer in the control fractions and the sample, after which the ratio of the pollen grains sizes of the sample and control fractions, expressed as a percentage, is determined, the higher this ratio, the more drought-resistant the genotype.
Description
Invenţia se referă la agricultură, în particular la metode de determinare a genotipurilor de tomate rezistente la secetă, care posedă o capacitate înaltă de reţinere a apei şi poate fi utilizată în cercetările geneticii de rezistenţă a plantelor, cât şi la crearea soiurilor şi hibrizilor noi pe baza utilizării însuşirilor şi criteriilor gametofitului masculin. The invention relates to agriculture, in particular to methods for determining drought-resistant tomato genotypes, which possess a high water-holding capacity and can be used in plant resistance genetics research, as well as in the creation of new varieties and hybrids on the basis of using the characteristics and criteria of the male gametophyte.
Este cunoscută metoda de estimare a capacităţii de reţinere a apei la etapa de sporofit pe baza utilizării diferitor părţi componente ale plantelor: frunze, spice în procesul de veştezire. Despre pierderea apei se constată pe baza rezultatelor cântăririi materialului experimental până şi după veştezire [1]. There is a known method of estimating the water retention capacity at the sporophyte stage based on the use of different component parts of the plants: leaves, ears in the germination process. Water loss is established based on the results of weighing the experimental material up to and after publication [1].
Metoda este destul de dificilă şi mulţi factori (umiditatea, temperatura solului şi aerului) influenţează asupra veridicităţii rezultatelor. The method is quite difficult and many factors (humidity, soil and air temperature) influence the veracity of the results.
Problema pe care o rezolvă această invenţie constă în posibilitatea evaluării şi selectării genotipurilor la etapele iniţiale de vegetaţie în baza capacităţii de reţinere a apei de grăuncioarele de polen. The problem that this invention solves is the possibility of evaluating and selecting genotypes at the initial stages of vegetation based on the water retention capacity of the pollen grains.
Metoda, conform invenţiei, include colectarea florilor complet deschise de fiecare genotip, separarea polenului din antere, divizarea polenului în două fracţii, fracţia martor se amplasează pe o lamă-portobiect uscată, iar fracţia de probă se amplasează pe o lamă-portobiect într-o picătură de soluţie de sorbit de 85% şi nivelul presiunii osmotice de 104,6 atm, timp de 2 ore, apoi la microscop se determină dimensiunile grăuncioarelor de polen cu ajutorul micrometrului ocular în fracţiile martor şi de probă, după care se determină raportul dimensiunilor grăuncioarelor de polen ale fracţiilor de probă şi martor, exprimat în procente, totodată cu cât este mai mare acest raport, cu atât genotipul este mai rezistent la secetă. The method, according to the invention, includes collecting the fully open flowers of each genotype, separating the pollen from the anthers, dividing the pollen into two fractions, the control fraction is placed on a dry slide, and the sample fraction is placed on a slide in a drop of sorbet solution of 85% and the osmotic pressure level of 104.6 atm, for 2 hours, then the sizes of the pollen grains are determined under the microscope with the help of the ocular micrometer in the control and sample fractions, after which the ratio of the grain sizes is determined of pollen of the sample and control fractions, expressed in percentages, at the same time, the higher this ratio, the more resistant the genotype is to drought.
Rezultatul constă în posibilitatea diferenţierii şi a selectării genotipurilor de tomate rezistente la secetă prin estimarea indicilor gametofitului masculin (polen). Metoda este simplă, autentică şi nu necesită cheltuieli mari. The result consists in the possibility of differentiating and selecting drought-resistant tomato genotypes by estimating the indices of the male gametophyte (pollen). The method is simple, authentic and does not require large expenses.
Exemplu de realizare a invenţiei Example of realization of the invention
Au fost utilizate 9 genotipuri de tomate: soiurile Victorina, Ceros - 70, Delta, Peto - 86, Novicioc, speciile spontane şi varietăţile semiculturale: L.cheesmanii, L.hirsutum, L.esc.var.racemigerum, L.esc.var.pruniforme. Plantele se cultivă în câmp după metoda cunoscută până la înflorire. De la fiecare genotip de pe 15…20 de plante se colectează florile complet deschise, se separă anterele, care se transferă în pachete de pergament pentru uscare uşoară în termostat cu regim termic de 25…26°C, timp de 6 ore. După expirarea termenului dat polenul se izolează din antere prin scuturarea mecanică. Polenul fiecărui genotip se divizează în două fracţii (martor şi probă), fracţia martor se amplasează pe o lamă-portobiect uscată, iar fracţia de probă se amplasează pe o lamă-portobiect într-o picătură de soluţie de sorbit de 85% şi nivelul presiunii osmotice de 104,6 atm, timp de 2 ore, apoi la microscop se determină dimensiunile grăuncioarelor de polen cu ajutorul micrometrului ocular în fracţiile martor şi de probă, după care se determină raportul dimensiunilor grăuncioarelor de polen ale fracţiilor de probă şi martor, exprimat în procente, totodată cu cât este mai mare acest raport, cu atât genotipul este mai rezistent la secetă, ce caracterizează modificările acestui indicator în dependenţă de pierderea apei din grăuncioarele de polen. Rezultatele obţinute sunt prezentate în tabel. 9 tomato genotypes were used: Victorina, Ceros - 70, Delta, Peto - 86, Novicioc varieties, spontaneous species and semi-cultivated varieties: L.cheesmanii, L.hirsutum, L.esc.var.racemigerum, L.esc.var .uniform. The plants are cultivated in the field according to the known method until flowering. From each genotype on 15...20 plants, the fully open flowers are collected, the anthers are separated, which are transferred to parchment packages for easy drying in a thermostat with a thermal regime of 25...26°C, for 6 hours. After the expiration of the given term, the pollen is isolated from the anthers by mechanical shaking. The pollen of each genotype is divided into two fractions (control and sample), the control fraction is placed on a dry slide, and the sample fraction is placed on a slide in a drop of 85% sorbitol solution and the pressure level osmotic pressure of 104.6 atm, for 2 hours, then the sizes of the pollen grains are determined under the microscope using the ocular micrometer in the control and sample fractions, after which the ratio of the sizes of the pollen grains of the sample and control fractions is determined, expressed in percentages, at the same time, the higher this ratio is, the more resistant the genotype is to drought, which characterizes the changes in this indicator depending on the loss of water from the pollen grains. The obtained results are presented in the table.
În baza datelor obţinute s-a constatat că genotipurile L.esc.var.pruniforme, L.esc.var.racemigerum şi soiul Peto - 86 au demonstrat valori maximale ale coraportului perimetrelor. Prin urmare, aceste genotipuri pot fi caracterizate cu o capacitate înaltă de reţinere a apei. Totodată specia spontană L.cheesmanii, soiul Novicioc şi mai ales L.hirsutum au manifestat coraportul perimetrelor cu mult mai scăzut. Based on the data obtained, it was found that the genotypes L.esc.var.pruniforme, L.esc.var.racemigerum and the variety Peto - 86 demonstrated maximum values of the perimeters coraport. Therefore, these genotypes can be characterized with a high water retention capacity. At the same time, the spontaneous species L.cheesmanii, the Novicioc variety and especially L.hirsutum showed a much lower perimeter correlation.
Tabel Table
Variabilitatea perimetrelor grăuncioarelor de polen la tomate Variability of tomato pollen grain perimeters
Genotip Perimetrul grăuncioarelor de polen în unităţi convenţionale Coraportul perimetrelor (martor şi probă), % Grupa de rezistenţă Martor Probă 1. L.esc.var.pruniforme 114,5±0,87 111,5±0,90 97,3 I 2. Peto - 86 114,8±0,87 109,3±0,91 95,2 3. L.esc.var.racemigerum 113,9±0,88 107,7±0,93 94,5 4.Delta 119,1±0,83 110,0±0,90 92,3 II 5.Victorina 114,0±0,87 104,6±0,95 91,7 6. Ceros - 70 116,7±0,85 107,0±0,93 91,6 7. L.cheesmanii 119,7±0,83 107,0±0,93 89,3 III 8. Novicioc 114,4±0,87 101,1±0,99 88,3 9. L.hirsutum 103,4±0,97 81,0±1,23 78,3 HCP0,5 2,01 Genotype The perimeter of the pollen grains in conventional units The ratio of the perimeters (control and sample), % Resistance group Control Sample 1. L.esc.var.pruniform 114.5±0.87 111.5±0.90 97.3 I 2 Peto - 86 114.8±0.87 109.3±0.91 95.2 3. L.esc.var.racemigerum 113.9±0.88 107.7±0.93 94.5 4.Delta 119.1±0.83 110.0±0.90 92.3 II 5. Victorina 114.0±0.87 104.6±0.95 91.7 6. Ceros - 70 116.7±0.85 107.0±0.93 91.6 7. L.cheesmanii 119.7±0.83 107.0±0.93 89.3 III 8. Novicioc 114.4±0.87 101.1±0.99 88.3 9. L.hirsutum 103.4±0.97 81.0±1.23 78.3 HCP0.5 2.01
Datele prezentate relevă că aceste genotipuri posedă o capacitate de reţinere a apei mai scăzută. Soiurile Victorina şi Ceros - 70 se caracterizează printr-un nivel mediu de reţinere a apei, deoarece coraportul perimetrelor acestora ocupă un loc intermediar între cele două grupe. The presented data reveal that these genotypes possess a lower water retention capacity. The varieties Victorina and Ceros - 70 are characterized by an average level of water retention, because the ratio of their perimeters occupies an intermediate place between the two groups.
În baza cercetărilor efectuate s-a stabilit că cu cât este mai mare capacitatea de reţinere a apei de către grăuncioarele de polen, cu atât genotipurile tomatelor sunt mai rezistente la secetă. Based on the research carried out, it was established that the higher the water retention capacity of the pollen grains, the more resistant the tomato genotypes are to drought.
1. Кожушко И. Н. Определение засухоустойчивости зерновых культур по изменению параметров водного режима. Ленинград, 1984, с. 3-13 1. Kozhushko И. N. Determination of drought resistance of grain cultures by changing the parameters of the water regime. Leningrad, 1984, p. 3-13
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20130036A MD653Z (en) | 2013-02-28 | 2013-02-28 | Method for determination of drought-resistant genotypes of tomato |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20130036A MD653Z (en) | 2013-02-28 | 2013-02-28 | Method for determination of drought-resistant genotypes of tomato |
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| Publication Number | Publication Date |
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| MD653Y MD653Y (en) | 2013-07-31 |
| MD653Z true MD653Z (en) | 2014-02-28 |
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Family Applications (1)
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| MDS20130036A MD653Z (en) | 2013-02-28 | 2013-02-28 | Method for determination of drought-resistant genotypes of tomato |
Country Status (1)
| Country | Link |
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| MD (1) | MD653Z (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD1033G2 (en) * | 1997-11-06 | 1999-07-31 | Институт Генетики И Физиологии Растений Академии Наук Молдовы | Method for selection of tomato genotypes resistent to salination |
| MD3374G2 (en) * | 2007-03-14 | 2008-03-31 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Method of selecting tomato heat resistant genotypes |
| MD3421G2 (en) * | 2007-04-06 | 2008-06-30 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Method of identifying the wheat genotypes resistant to root rot pathogenic agents |
| MD3638G2 (en) * | 2008-02-11 | 2009-02-28 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Method of selecting tomato cold resistant genotypes |
| MD3739G2 (en) * | 2008-03-14 | 2009-06-30 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Method for selecting drought resistant tomato genotypes |
| MD45Z (en) * | 2009-04-23 | 2010-02-28 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Method for selecting tomato genotypes resistant to higher temperatures |
| MD55Z (en) * | 2009-03-13 | 2010-03-31 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Method for selecting drought-resistant tomato genotypes |
| MD56Z (en) * | 2009-03-31 | 2010-03-31 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Method for selecting salinization-resistant tomato genotypes |
-
2013
- 2013-02-28 MD MDS20130036A patent/MD653Z/en not_active IP Right Cessation
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD1033G2 (en) * | 1997-11-06 | 1999-07-31 | Институт Генетики И Физиологии Растений Академии Наук Молдовы | Method for selection of tomato genotypes resistent to salination |
| MD3374G2 (en) * | 2007-03-14 | 2008-03-31 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Method of selecting tomato heat resistant genotypes |
| MD3421G2 (en) * | 2007-04-06 | 2008-06-30 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Method of identifying the wheat genotypes resistant to root rot pathogenic agents |
| MD3638G2 (en) * | 2008-02-11 | 2009-02-28 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Method of selecting tomato cold resistant genotypes |
| MD3739G2 (en) * | 2008-03-14 | 2009-06-30 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Method for selecting drought resistant tomato genotypes |
| MD55Z (en) * | 2009-03-13 | 2010-03-31 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Method for selecting drought-resistant tomato genotypes |
| MD56Z (en) * | 2009-03-31 | 2010-03-31 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Method for selecting salinization-resistant tomato genotypes |
| MD45Z (en) * | 2009-04-23 | 2010-02-28 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Method for selecting tomato genotypes resistant to higher temperatures |
Non-Patent Citations (4)
| Title |
|---|
| В.С. Смирнова, И.В.Гаранько «Диагностикахолодостойкости образцов культурного томата» Методические указания, Ленинград 1990 с.24 * |
| Кожушко И. Н. Определение засухоустойчивости зерновых культур по изменению параметров водного режима. Ленинград, 1984, с. 3-13 * |
| Методические указания по определению жаростойкости овощных культур по ростовой реакции проростков после прогревания их при высокой температуре (томаты). Ленинград, Всесоюзная ордена Ленина академия сельскохозяйственных наук имени В.И. Ленина, Всесоюзный ордена Ленина и ордена Дружбы народов научно-исследовательский институт растениеводства имени Н.И. Вавилова, 1979 * |
| Определение солеустойчивости овощных культур по прорастанию семян в солевых растворах. Методические указания. Ленинград, 1986, c. 15 * |
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| Publication number | Publication date |
|---|---|
| MD653Y (en) | 2013-07-31 |
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