CN108663481A - A kind of field symptom fast diagnosis method of tomato yellow leaf droop - Google Patents
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Abstract
The present invention provides a kind of method of quick diagnosis tomato yellow leaf droop, this method includes the appearance from tomato leaf to aerial root, to identify yellow leaf droop providing method in early days, several illnesss of the invention mainly for various similar symptoms, quick, simple, effective diagnostic method is provided, to realize that the early of disease finds in tomato production, the principle early prevented, the developmenting spread of the effective control state of an illness, to play the purpose of disease-preventing and yield-increasing.
Description
Technical field
The invention belongs to plant disease diagnosis, in particular, it is quick to belong to a kind of field symptom to tomato yellow leaf droop
Diagnostic method.
Background technology
Tomato (Lycopersicon esculentum Mill.) is annual or herbaceos perennial, originates in South America
Continent, present north and south of China are cultivated extensively, are one of fruits and vegetables indispensable in current agricultural production.Because the fruit of tomato is rich in
Vitamin, minerals, carbohydrate, organic acid and a small amount of protein, have promote digestion, diuresis, inhibition various bacteria
It acts on, vitamin D can protect blood vessel in tomato, control hypertension.There is glutathione in tomato, there is postponement cell ageing, increases people
Body anti-cancer ability.The various ways such as its fruit can eat raw, cook, catsup processing, juice or whole fruit canning.It is in agricultural production
Important industrial crops.
Zhejiang Province Cangnan tomato base is one of certain scale, the higher south of degree of specialization " autumn plants spring receipts type "
Greenhouse tomato base.However, there is a kind of plant leaf from bottom to top in Zhejiang Province Cangnan base tomato field in recent years
Withered and yellow, adventitious root is apparent, rear withered disease in blocks, and production loss is very big；The disease in Zhejiang Province Cangnan base wide-scale distribution,
Serious field incidence is up to 80% or more.The disease is referred to as tomato yellow leaf droop, is caused by sickle-like bacteria.In field, easily
Obscure with other several pathology or physiological disturbance with similar symptoms, present invention is generally directed to the several of various similar symptoms
Disease provides quick, simple, effective diagnostic method, to realize that the early of disease finds in tomato production, the original early prevented
Then, the developmenting spread for effectively controlling the state of an illness, to play the purpose of disease-preventing and yield-increasing.
Invention content
The present invention provides a kind of method of quick diagnosis tomato yellow leaf droop, and this method includes:
Step 1:First, from the time that tomato field planting is generated to the first inflorescence, it is single to answer Ye Zhongyi from plant bottommost
There is yellowing phenomenon at first in the mesophyll edge of leaf, is then slowly expanded to entire single leaf, it is in Huang that the vein symptom of blade, which is vein,
Green even sepia；Over time, blade is gradually withered, and rear sprawling extends to other single leaves nearby, until entire
Compound leaf yellow is withered；
Step 2:Secondly, when plant is grown to the first inflorescence between third inflorescence, the state of an illness is gradually aggravated, and occurs palm fibre in vein
The arteries and veins line of brown, network-like to be distributed between single leaf vein of multiple yellows, there is browning phenomenon, brown arteries and veins line in subsequent many places mesophyll
Stem is reached from vanes petiole, brown scab also occurs in stem；Scab extends to other blades up and down therewith, in the leaf of yellow
Stem between position and healthy leaf position easily grows a large amount of aerial roots, with being further aggravated for the state of an illness, stem's gas near sick leaf
Growth more crypto set of taking root is vigorous, and the growth position of aerial root is gradually moved up along stem；According to these symptoms, Jin Erke
To be determined as tomato yellow leaf droop.
Further include step 3 in some preferred modes:Finally, disease is developed to after third inflorescence occurs in plant：
There is yellow wilt phenomenon in 1/3 to 1/2 blade, and entire plant strain growth is slow, plant top occur withering here symptom, some stems
With apparent brown scab；Vertical profile basal part of stem, vascular bundle is in yellowish-brown, and the rigid jaundice of root system, capillary root are rare；Finally
The withered browning of whole plant is dead.
Advantageous effect
Invention provides quick, simple, effective diagnostic method mainly for several illnesss of various similar symptoms, to
Realize that the early of disease finds in tomato production, the principle early prevented effectively controls the developmenting spread of the state of an illness, anti-to play
The purpose of disease volume increase.
Description of the drawings
Symptom (from left to right, illness degree is deepened) figure of Fig. 1 tomato yellow leaf droops.
Fig. 2 tomato magnesium deficiency symptom features performance figure.
Fig. 3 tomato fertilizer damage features performance figure
Fig. 4 bacterial wilt of tomato symptom figures.
Specific implementation mode
Embodiment 1
The acquisition of 1.1 various tomato disease samples
In October, 2015 in April, 2016, Tong Chu village of in the September, 2016 in January, 2017 in Cangnan Long Harbour Town, new light
Village, the villages San great Miao, the villages such as the river mouth village Ye Cun, Nei Li in the towns Ling Xi sample doubtful tomato yellow leaf disease plant, adopt respectively
The various tomato yellow leafs of A, B, C, D etc. and the sick sample of withered illness are collected, have amounted to 231 parts, be packed into clean freshness protection package and take back examination
Test indoor carry out Symptom Observation and description.
1.2 field Symptom Observations：
The A sample Symptom Observation results of acquisition：
Out of, tomato field planting is generated to the first inflorescence time, the mesophyll edge of the single leaf of Ye Zhongyi is answered from plant bottommost
Occur yellowing phenomenon at first, is then slowly expanded to entire single leaf, the vein symptom of blade is that vein is even brown in yellow green
Color；Over time, blade is gradually withered, and rear sprawling extends to other single leaves nearby, until entire compound leaf yellow is withered；
Secondly, when plant is grown to the first inflorescence between third inflorescence, the state of an illness is gradually aggravated, and occurs sepia in vein
Arteries and veins line, network-like to be distributed between single leaf vein of multiple yellows, there is browning phenomenon in subsequent many places mesophyll, and brown arteries and veins line is from blade
Stem is reached through petiole, brown scab also occurs in stem；Scab extends to other blades up and down therewith, in the leaf position of yellow and is good for
Stem between Kang Yewei easily grows a large amount of aerial roots, with being further aggravated for the state of an illness, stem's aerial root life near sick leaf
Long more crypto set is vigorous, and the growth position of aerial root is gradually moved up along stem；
Finally, disease is developed to after third inflorescence occurs in plant：There is yellow wilt phenomenon in 1/3 to 1/2 blade,
Entire plant strain growth is slow, plant top occur withering here symptom, some stems have apparent brown scab；Vertical profile basal part of stem,
Vascular bundle is in yellowish-brown, and the rigid jaundice of root system, capillary root are rare；The last withered browning of whole plant is dead.
The B sample Symptom Observation results of acquisition
In squaring period plant lower part, there is jaundice chlorosis in old leaf, forms clear reticulate pattern floral leaf mostly after chlorisis, vein is protected
Stay green, along master pulse both sides in patch chlorisis and leaf margin does not take off, it is leaf complete；Also part chlorisis since leaf margin prolongs to middle rib
Exhibition, the extension of backward upper leaf form chrysanthemum variegated leaf, serious blade is slightly stiff or edge on roll up, occur between vein necrotic plaque or
Brown block band is formed between vein, causes blade withered or whole leaf is to complete stool yellow.Symptom development is slow.
The C sample Symptom Observation results of acquisition
There is the white of chlorisis or the irregular spot of canescence at first in blade surface, and with progression of the disease, spot is extended to one
Fixed degree stops extension.The withered spot edge having on blade is canescence, and internal mesophyll has little time chlorisis, deeper in color
Withered green, later stage perforation.Progression of the disease is very rapid under high temperature, drought condition, and leaf margin is dried-up, and here a large amount of blades wither.
The D disease sample Symptom Observation results of acquisition
Top blade was wilted sagging before this, and rear lower blade is wilting, and middle part blade is finally wilting, also has side blade first to wither
What listless or whole strain blade was wilted simultaneously, early stage, diseased plant is wilted daytime, is restored at dusk, sick leaf lighter.Antecedent soil moisture,
Temperature is higher, 2~3 days complete stools, that is, wilting.If temperature is relatively low, when cloudy weather for several days running or higher soil moisture content, diseased plant is after sustainable 1 week
It is withered, but blade still keeps green or slightly weaker, cuts off base of the plant, firmly pinching stem wound site, it is seen that and bacterial ooze flows out, and
With rotten stink.
The separation of the cause of disease of 1.3 various symptoms is identified
The separation of pathogen：Sick sample is detached using tissue isolation.To each (tetra- kinds of A, B, C, D of acquisition
Class) disease of tomato disease sample is good for intersection and is cut into the tissue blocks of 2mm × 2mm sizes with scissors, after 70% ethyl alcohol surface sterilization 1min
It is embathed 3 times with sterile water, 25 DEG C of stationary cultures on PDA culture medium tablet is positioned over after sample surfaces drying.Wait for that bacterium colony is formed,
Purifying culture is carried out with transfer needle picking mycelia block.
As a result, it has been found that：It is white that A disease samples, which detach in PDA culture medium and obtain a kind of bacterium colony, villous fungi separation
Object, microscope inspection find its generate microconidia be oval to oval, colourless monospore, size 2.93~6.46 ×
14.01~24.1um；Macroconidium is sickle-shaped or long spindle, colourless, there is a diaphragm, and size 4.7~6.97 × 25.23~
41.12um.Morphological feature is Fusarium oxysporum.B disease samples detach in PDA culture medium does not obtain pathogen separation object.C disease samples exist
It is detached in PDA culture medium and does not obtain pathogen separation object.D disease samples detach in PDA culture medium obtains a kind of bacterium isolate, the bacterium
Bacterium colony is round, the smooth of the edge, marshy land shape.
1.4 Pathogenicity
Use hinder root root-pouring method to tomato (kind for：Zhejiang powder 502) healthy plant carry out pathogen separation object pathogenic survey
It is fixed.Cause of disease is respectively the pathogen separation object bacterial strain (FB-1-1) of A symptoms and the pathogen separation object bacterial strain (FB-K1) of D symptoms.FB-
1-1 collects spore after cultivating 7d in liquid PDA culture medium, and 0.1%CMC-Na aqueous solutions is used in combination to be diluted to 106A/mL is used for
Inoculation test；Picking FB-K1 single bacterium colonies on NA fluid nutrient mediums shaken cultivation for 24 hours after, be diluted to a concentration of OD600 be 0.8
When it is for use.The tomato potting seedling for choosing health, basal part of stem is poured into after hindering root with blade by the uniform suspension of two kinds of isolates
In soil, sterile water inoculation is check experiment.It is inoculated with 10 pottings altogether, is positioned in 28 DEG C of illumination boxs and protects after inoculation processing
Wet culture, observes and records incidence daily.
It is found after inoculation 7-20, the tomato plant growing way of FB-1-1 inoculations is weak, radical leaves flavescence, browning, from compound leaf
End of blade to petiole spread, the blade of diseased plant is bottom-up withered and yellow, and plant site of pathological change adventitious root is apparent, and pulling up diseased plant can find
Root system necrosis.And here the plant initial stage lobus cardiacus of FB-K1 inoculations withers, diseased plant is wilted daytime, is restored at dusk, sick leaf lighter.With
Entire plant withered death afterwards.The inoculation symptom of both pathogen separation objects is all identical as field symptom.It is thus regarded that the cause of disease
Isolate is the pathogen for leading to two kinds of diseases.
The molecular biology identification of 1.5 pathogen separation objects
Representative isolate bacterial strain is forwarded on PDA plate, after 25 DEG C of culture 7d, scrapes media surface bacterium
Filament, using the genome of CTAB methods extraction pathogen separation object after being crushed pathogen cell wall with liquid nitrogen.Fungi separator uses
The ITS regional sequences of ITS1 and ITS4 primer pair bacterial strains carry out PCR amplification, and bacterium isolate uses 7F and 1540R primer pairs
16SrDNA carries out PCR amplification.PCR product is purified and is sequenced by Shanghai bioengineering Co., Ltd.Sequencing result and GenBank
Middle sequence carries out sequence analysis, and is committed to GenBank databases.
The ITS sequence of A symptom isolates expands, and obtains the sequence (sequence summary) that length is 515bp, D symptom isolates
16SrDNA sequence amplifications obtain the sequence (sequence summary) that length is 338bp.The sequence carries out sequence alignment on Genbank,
As a result, it has been found that it is 100% that the isolate bacterial strain of A illnesss, which is with the sequence similarity of Fusarium oxysprum, and point of D illnesss
From object bacterial strain be with the sequence similarity of Ralstonia solanacearum it is 100%.
It is thus regarded that the pathogen separation object bacterial strain (FB-1-1) of A symptoms is Fusarium oxysporum, the pathogen separation object bacterium of D symptoms
Strain (FB-K1) is ralstonia solanacearum.To which A illnesss are tomato yellow leaf droop, D illnesss are bacterial wilt of tomato.
The cause of disease of 1.6 B illnesss and C illnesss measures
The soil disease sample for taking part B illnesss and C illnesss is sent after straw impurity is filtered in grinding to Zhejiang Province's agriculture
Institute of section ring soil carries out the measurement (carbon C, nitrogen N, phosphorus P, sulphur S, potassium K, magnesium Mg, calcium Ca, silicon Si) of a large amount of infrastructure elements.As a result, it has been found that
Carbon, nitrogen phosphate and sulfur, potassium, calcium and element silicon index are qualified in the soil sample of B symptoms, and the content of magnesium elements is very low, therefore B illnesss
For grass tetany；And carbon in the soil sample of C disease samples, phosphorus, sulphur, potassium, magnesium, the content of calcium and silicon are qualified, but nitrogen content severely exceeds, because
This, C symptoms excessively cause for nitrogenous fertilizer.Simultaneously according to the method described above, the cause of disease of C same symptoms (E and F) is detected,
It point is found phosphorus and potassium is exceeded, illustrate that nitrogen, phosphorus and potassium severely exceed the symptom that can also cause as C samples.
Embodiment 2
2.1 materials and methods
2.1.1 tomato variety and material processing
The soil for preparing disease-free normal soil and magnesium deficiency respectively is dried after 121 DEG C of damp and hot disinfections with 80 DEG C, dress
Basin.Normal soil setting fertilizer excessive (200g ternary compound fertilizer/basin), inoculation bacterial wilt (Ralstonia solanacearum FB-K1, bacterium solution
A concentration of 1 × 108Cfu/ml) and droop (sickle-like bacteria FB-1-1,1 × 106A/ml) etc. processing, magnesium deficiency soil be individually place
Reason is control with sterile water, 3 repetitions of each processing setting, and conceals the set label handled, is indicated respectively with code name：S-
1-15.Each 1 tomato planting stock of potted plant growth (Zhejiang powder 502, three leaves are wholeheartedly).Potting is placed in greenhouse culture, and temperature is
20℃-30℃.Cultivation management is carried out by normal management mode.Observe and record tomato growth and incidence daily.
2.1.2 the separation identification of pathogen separation object：Sick sample is detached using tissue isolation.To the tomato disease of acquisition
The disease of sample is good for the tissue block that intersection is cut into 2mm × 2mm sizes with scissors, with sterile water logging after 70% ethyl alcohol surface sterilization 1min
It washes 3 times, 25 DEG C of stationary cultures on PDA culture medium tablet is positioned over after sample surfaces drying.It waits for that bacterium colony is formed, is chosen with transfer needle
Mycelia block is taken to carry out purifying culture and identification.
2.1.3 the Pathogenicity of pathogen separation object
Use root-pouring method to tomato (kind for：Zhejiang powder 502) healthy plant carry out pathogen separation object Pathogenicity.It will
Spore (10 is collected after pathogen separation object strain culturing6A/mL) or bacterium solution (OD600=0.8), it is used for inoculation test.It chooses strong
The tomato potting seedling of health, uniformly pours into spore suspension or bacterium solution in basal part of stem soil after hindering root with blade, sterile water inoculation
For check experiment.It is inoculated with 10 pottings altogether, moisturizing culture in 28 DEG C of illumination boxs is positioned over after inoculation processing, daily observation is simultaneously
Record incidence.
2.1.4 the molecular biology identification of pathogen separation object
Representative isolate bacterial strain is forwarded on PDA plate, after 25 DEG C of culture 7d, scrapes media surface bacterium
Filament, using the genome of CTAB methods extraction pathogen separation object after being crushed pathogen cell wall with liquid nitrogen.Fungi separator uses
The ITS regional sequences of ITS1 and ITS4 primer pair bacterial strains carry out PCR amplification, and bacterium isolate uses 7F and 1540R primer pairs
16SrDNA carries out PCR amplification.PCR product is purified and is sequenced by Shanghai bioengineering Co., Ltd.Sequencing result and GenBank
Middle sequence carries out sequence analysis.
2.2 results and analysis
2.2.1 the field symptom description respectively handled
After plantation 7 days, the processing of set tomato S-4,5,6 and S-10,11,12 plant a small amount of blade occur withering here symptom,
There is a phenomenon where green withered for the subsequent whole strain of these tomatoes.Diseased plant tomato stem is cut, stem is firmly squeezed, S-11,12,13 are visible big
When dripping muddy bacterial ooze outflow, and have rotten stink, and squeezing the sick stem of S-4,5,6, only a small amount of Tomato Juice is overflowed,
With the dense distinctive fragrance of tomato.Other plant strain growths are normal.
When plantation 3 weeks, plant grows to 20cm or so, and a leaflet of the bottom of S-1,2,3 compound leaf leaf margin occurs and turns to be yellow symptom,
The yellowish green alternate symptoms such as similar bright arteries and veins are subsequently changed to, with plant growth, slowly sprawling is expanded to entire blade to flavescence blade,
Or multiple blades.Entire plant strain growth is slow, short and small.
Plantation 4 weeks when, tomato plant grows first spray and pollination of blooming successively, the least significant end of S-7,8,9 it is a piece of
There is yellowing phenomenon in leaflet, and amplification is slow, and entire compound leaf turns yellow after about 1-2 weeks, and vein has the symptom of browning, its remaining part of plant
Divide well-grown, with the development of the state of an illness, the blade quantity of flavescence increases, and develops upwards, and growth slows down, and brown disease occurs in stem
Spot, the neighbouring stem aerial root raised growth of sick leaf.
S-1,2,3 and S-7,8,9 progression of the disease speed it is slow, about 1-2 months, whole yellow of plant was withered.Split this
The basal part of stem of a little plant, it is possible to find stem's vascular bundle brown stain of S-7,8,9 is apparent, root system jaundice, and capillary root is rare.And S-1,2,
3 vascular bundle is without browning phenomenon, and root system is vigorous, and capillary is high-visible.
Based on above symptom characteristic, it can be determined that S-4,5,6 are fertilizer damage, and S-11,12,13 may be that inoculation is green withered
Disease processing, S-1,2,3 and S-7,8,9 are respectively tomato magnesium deficiency and droop processing.
2.2.2 the separation identification of pathogen
The diseased plant to each processing described above and soil carry out the separation identification (Morphological Identification of pathogen respectively respectively
And Molecular Identification) and macronutrient measurement, as a result such as following table：
Therefore, growth period, the disease disease of corresponding plant occur according to yellow leaf droop, bacterial wilt, magnesium deficiency and fertilizer damage
Shape is specific and the speed of Occurrence and development of disease etc. can become several physiologicals of field diagnosis tomato or pathologic condition according to
According to quickly to be coped with.To early discovery, the principle early prevented, the effective developmenting spread for controlling the state of an illness, to rise
To the purpose of disease-preventing and yield-increasing.
2. the description of the classical symptom of 2.3 various diseases
Tomato yellow leaf droop：
This period that the disease generates after field planting to the first inflorescence takes place successively, and Ye Zhongyi is answered from plant bottommost
The edge of a list leaf shows disease at first, yellowing phenomenon occurs, is then slowly expanded to entire single leaf, and symptom is turned to be yellow for mesophyll, vein
In yellow green even sepia；Over time, blade is gradually withered, and rear sprawling extends to other single leaves nearby, until
Entire compound leaf yellow is withered.
When plant is grown to the first inflorescence between third inflorescence, the state of an illness is gradually aggravated, and occurs the arteries and veins line of sepia in vein,
Network-like to be distributed between single leaf vein of multiple yellows, there is browning phenomenon in subsequent many places mesophyll, and brown arteries and veins line is from vanes leaf
Handle reaches stem, and brown scab also occurs in stem；Scab extends to other blades up and down therewith, in the leaf position of yellow and healthy leaf
Stem between position easily grows a large amount of aerial roots, and with being further aggravated for the state of an illness, stem's aerial root growth near sick leaf is more
Crypto set is vigorous, and the growth position of aerial root is gradually moved up along stem.This when, so that it may to judge the tomato as reaping hook
Microbial yellow leaf disease, so that it may to carry out chemical control.
Disease is developed to after third inflorescence occurs in plant：There is yellow wilt phenomenon in 1/3 to 1/2 blade, entirely
Plant strain growth is slow, plant top occur withering here symptom, some stems have apparent brown scab.Vertical profile basal part of stem, dimension pipe
Beam is in yellowish-brown, and the rigid jaundice of root system, capillary root are rare；The last withered browning of whole plant is dead.This can be further
Confirm disease, although this when of diseases prevention medication is slightly a little later, these plants can be cleared up and go to be focused on,
Prevention continues to propagate next time, and those are not plant (such as the physiology venereal diseases such as fertilizer damage or nutritional deficiency of fungi and bacterium
Evil) crop field can be stayed in as fertilizer, prevention waste.
Magnesium deficiency illness：
There is jaundice chlorosis in first greenhouse expanding stage plant lower part old leaf, forms clear reticulate pattern flower after chlorisis mostly
Leaf, main survey arteries and veins and thready pulse retain green, along master pulse both sides in patch chlorisis and leaf margin does not take off, it is leaf complete；Also have part from
Leaf margin starts chlorisis and extends to middle rib, the extension of backward upper leaf, forms chrysanthemum variegated leaf, serious blade is slightly stiff or edge on
Volume occurs necrotic plaque between vein or forms brown block band between vein, causes blade withered or whole leaf is to complete stool yellow.(with it is withered
The difference of disease is no pathogen, no vascular bundle browning, the generation without a large amount of aerial roots).
Fertilizer damage symptom：
There is the white of chlorisis or the irregular spot of canescence in initial stage, blade surface, and with progression of the disease, spot expands.Leaf
The withered spot edge that on piece has is canescence, and internal mesophyll has little time chlorisis, is in the deeper withered green of color, later stage perforation.With disease
Unlike evil, withered spot will not infinitely expand.Progression of the disease is very rapid under high temperature, drought condition, and leaf margin is dried-up, great Liang Ye
Here piece withers.(being similar to green withered) bacterial wilt illness：Top blade was wilted sagging before this, and rear lower blade is wilting, and middle part blade is most
It is wilting afterwards, also there is side blade first to wilt or whole strain blade while wilting, early stage, diseased plant daytime wilts, restore at dusk,
Sick leaf lighter.After the onset, antecedent soil moisture, temperature is higher, 2~3 days complete stools, that is, wilting.If temperature is relatively low, cloudy weather for several days running or soil
When earth water content is higher, diseased plant is withered after sustainable 1 week, but blade still keeps green or slightly weaker, therefore claims bacterial wilt.Cut off plant
Stem, firmly pinching stem wound site, it is seen that bacterial ooze flows out, and with rotten stink.
The field symptom comparison in difference of 1.3 tomato yellowing droops and other diseases
Tomato yellowing droop, magnesium deficiency, fertilizer damage or bacterial wilt field symptom have apparent difference, specifically refer to down
Table.
The terms and expressions mode for being used herein to description method is not unique constant, and we do not have any meaning
Figure excludes any mutually convertible expression way of the description present invention or feature using these terms and expressions modes, we
Accept a variety of different expression ways in the range of the present invention states.It is therefore believed that although of the invention herein
With various concrete schemes and arbitrary feature description clearly demonstrate that come, but change design disclosed herein table
Those experienced professional technique personages, and the statement one that these changes will be subsidiary with the present invention are also sought help from up to mode
It causes.Article, patent, content and the useful electronization referred to herein and citation of patent application and all other document
What information was bound together, it is necessary to be referred to as a complete content, delivering one part of any of which will be special
Indicate this point.Applicant has the information and material of these any and whole articles, patent, patent application or other documents
Material is merged into the right for the part that the application is disclosed as patent specification.
Claims (2)
1. a kind of method of quick diagnosis tomato yellow leaf droop, this method include:
Step 1：First, from the time that tomato field planting is generated to the first inflorescence, the single leaf of Ye Zhongyi is answered from plant bottommost
There is yellowing phenomenon at first in mesophyll edge, is then slowly expanded to entire single leaf, it is in yellow green that the vein symptom of blade, which is vein,
Even sepia；Over time, blade is gradually withered, and rear sprawling extends to other single leaves nearby, until entire compound leaf
Yellow is withered；
Step 2：Secondly, when plant is grown to the first inflorescence between third inflorescence, the state of an illness is gradually aggravated, and occurs sepia in vein
Arteries and veins line, network-like to be distributed between single leaf vein of multiple yellows, there is browning phenomenon in subsequent many places mesophyll, and brown arteries and veins line is from leaf
Piece reaches stem through petiole, and brown scab also occurs in stem；Scab extends to other blades up and down therewith, in the Ye Weiyu of yellow
Stem between healthy leaf position easily grows a large amount of aerial roots, with being further aggravated for the state of an illness, stem's aerial root near sick leaf
It is vigorous to grow more crypto set, and the growth position of aerial root is gradually moved up along stem；According to these symptoms, and then can be true
It is set to tomato yellow leaf droop.
2. according to the method described in claim 1, further including step 3:Finally, disease be developed to plant occur third inflorescence it
Afterwards：There is yellow wilt phenomenon in 1/3 to 1/2 blade, and entire plant strain growth is slow, plant top occur withering here symptom, have
Stem has apparent brown scab；Vertical profile basal part of stem, vascular bundle is in yellowish-brown, and the rigid jaundice of root system, capillary root are rare；
The last withered browning of whole plant is dead.
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| CN111567351A (en) * | 2020-04-30 | 2020-08-25 | 广西壮族自治区农业科学院 | Sugarcane seedling yellowing disease grading and preventing method |
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