MD461Z - Procedeu de obţinere a somaclonelor de varză - Google Patents
Procedeu de obţinere a somaclonelor de varzăInfo
- Publication number
- MD461Z MD461Z MDS20110099A MDS20110099A MD461Z MD 461 Z MD461 Z MD 461Z MD S20110099 A MDS20110099 A MD S20110099A MD S20110099 A MDS20110099 A MD S20110099A MD 461 Z MD461 Z MD 461Z
- Authority
- MD
- Moldova
- Prior art keywords
- somaclones
- cabbage
- murashige
- followed
- morphogenic
- Prior art date
Links
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Invenţia se referă la biotehnologie, în particular la un procedeu de obţinere a somaclonelor de varză.Procedeul include infectarea mecanică a plantelor în faza de două frunzuliţe cotiledonate cu virusul mozaicul conopidei, colectarea explantelor de ţesut mezofil din sectoarele învecinate zonelor clorotice, sterilizarea lor, inocularea explantelor pe mediul nutritiv Murashige şi Skoog, ce conţine agar 7 g/l, chinetină 0,01 mg/l şi suplimentar 6-benzilaminopurină 0,5 mg/l, acid naftilacetic 0,5 mg/l, cu incubarea ulterioară a lor la întuneric la temperatura de 25°C pentru iniţierea formării calusului morfogen, apoi cu o fotoperioadă de 16 ore la iluminare de 2000 lx şi temperatura de 27°C, transferarea calusurilor morfogene pe medii nutritive proaspete cu obţinerea somaclonelor şi înrădăcinarea ulterioară a lor pe mediul nutritiv Murashige şi Skoog, ce conţine suplimentar acid naftilacetic 0,5 mg/l cu adaptarea ulterioară la condiţii ex-vitro şi plantarea în sol a somaclonelor.Rezultatul invenţiei constă în sporirea variabilităţii somaclonale la varză şi în posibilitatea selecţiei somaclonelor care posedă caractere cantitative, nespecifice pentru soiul iniţial.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20110099A MD461Z (ro) | 2011-06-03 | 2011-06-03 | Procedeu de obţinere a somaclonelor de varză |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20110099A MD461Z (ro) | 2011-06-03 | 2011-06-03 | Procedeu de obţinere a somaclonelor de varză |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MD461Y MD461Y (ro) | 2012-01-31 |
| MD461Z true MD461Z (ro) | 2012-08-31 |
Family
ID=45815355
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MDS20110099A MD461Z (ro) | 2011-06-03 | 2011-06-03 | Procedeu de obţinere a somaclonelor de varză |
Country Status (1)
| Country | Link |
|---|---|
| MD (1) | MD461Z (ro) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1323046A1 (ru) * | 1984-11-29 | 1987-07-15 | Всесоюзный селекционно-генетический институт | Способ селекции люцерны на устойчивость к инфекционным заболевани м |
| SU1729337A1 (ru) * | 1990-04-16 | 1992-04-30 | Всесоюзный Научно-Исследовательский Институт Кукурузы | Способ первичного семеноводства гибридов кукурузы |
| MD722G2 (ro) * | 1996-09-20 | 1997-12-31 | Институт Физиологии Растений Академии Наук Республики Молдова | Procedeu de obţinere a embriozilor somatici |
| MD3899C2 (ro) * | 2009-02-23 | 2009-12-31 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Procedeu de micropropagare a Echinacea purpurea L. Moench in vitro |
-
2011
- 2011-06-03 MD MDS20110099A patent/MD461Z/ro not_active IP Right Cessation
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1323046A1 (ru) * | 1984-11-29 | 1987-07-15 | Всесоюзный селекционно-генетический институт | Способ селекции люцерны на устойчивость к инфекционным заболевани м |
| SU1729337A1 (ru) * | 1990-04-16 | 1992-04-30 | Всесоюзный Научно-Исследовательский Институт Кукурузы | Способ первичного семеноводства гибридов кукурузы |
| MD722G2 (ro) * | 1996-09-20 | 1997-12-31 | Институт Физиологии Растений Академии Наук Республики Молдова | Procedeu de obţinere a embriozilor somatici |
| MD3899C2 (ro) * | 2009-02-23 | 2009-12-31 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Procedeu de micropropagare a Echinacea purpurea L. Moench in vitro |
Non-Patent Citations (1)
| Title |
|---|
| În vitro technology for mutation breeding. A technical document issued by the International Atomic Energy Agency, IAEA-TECDOC 392, Vienna, 1986, p. 9-10, 12-14, 34, 47 * |
Also Published As
| Publication number | Publication date |
|---|---|
| MD461Y (ro) | 2012-01-31 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| KA4Y | Short-term patent lapsed due to non-payment of fees (with right of restoration) |