MD722G2 - Procedeu de obţinere a embriozilor somatici - Google Patents
Procedeu de obţinere a embriozilor somaticiInfo
- Publication number
- MD722G2 MD722G2 MD96-0257A MD960257A MD722G2 MD 722 G2 MD722 G2 MD 722G2 MD 960257 A MD960257 A MD 960257A MD 722 G2 MD722 G2 MD 722G2
- Authority
- MD
- Moldova
- Prior art keywords
- callus
- somatic
- cultivation
- medium
- panax ginseng
- Prior art date
Links
- 230000000392 somatic effect Effects 0.000 title abstract 4
- 238000004519 manufacturing process Methods 0.000 title abstract 3
- 206010020649 Hyperkeratosis Diseases 0.000 abstract 5
- 240000004371 Panax ginseng Species 0.000 abstract 3
- 235000002789 Panax ginseng Nutrition 0.000 abstract 3
- 235000008434 ginseng Nutrition 0.000 abstract 3
- 239000002609 medium Substances 0.000 abstract 3
- 238000012423 maintenance Methods 0.000 abstract 2
- 241000196324 Embryophyta Species 0.000 abstract 1
- 239000013543 active substance Substances 0.000 abstract 1
- 239000012737 fresh medium Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 239000013589 supplement Substances 0.000 abstract 1
- 238000002054 transplantation Methods 0.000 abstract 1
- 230000035899 viability Effects 0.000 abstract 1
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Invenţia se referă la agricultură şi poate fi utilizată în biotehnologie pentru obţinerea “seminţelor artificiale” din celulele calusului de Panax ginseng (C.A.Meyer).Esenţa invenţiei constă în aceea că se propune un procedeu de obţinere a embrioizilor somatici, care include obţinerea calusului din explantele rădăcinilor de Panax ginseng (C.A.Meyer) şi menţinerea viabilităţii lui pe mediul Murashige-Skoog cu adaos de substanţe biologic active prin transplantare peste fiecare 4-5 săptămâni de cultivare pe mediu proaspăt, transferarea calusului pe mediu, care conţine suplimentar în calitate de factor stimulator al embriogenezei epibrasinolidă în concentraţie de 10-6-10-5%, cultivarea calusului timp de 40-60 de zile cu o fotoperioadă de 16 ore lumină şi 8 ore întuneric, transferarea lui pe mediul Murashige-Skoog cu un conţinut redus de macro- şi microelemente, pe care după 75-90 de zile de cultivare apar embrioizi somatici.Rezultatul tehnic al invenţiei constă în menţinerea nelimitată a capacităţii calusului de Panax ginseng de a forma embrioizi somatici capabili de a genera plante viabile.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MD96-0257A MD722G2 (ro) | 1996-09-20 | 1996-09-20 | Procedeu de obţinere a embriozilor somatici |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MD96-0257A MD722G2 (ro) | 1996-09-20 | 1996-09-20 | Procedeu de obţinere a embriozilor somatici |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MD722F1 MD722F1 (ro) | 1997-05-31 |
| MD722G2 true MD722G2 (ro) | 1997-12-31 |
Family
ID=19738901
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MD96-0257A MD722G2 (ro) | 1996-09-20 | 1996-09-20 | Procedeu de obţinere a embriozilor somatici |
Country Status (1)
| Country | Link |
|---|---|
| MD (1) | MD722G2 (ro) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD461Z (ro) * | 2011-06-03 | 2012-08-31 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Procedeu de obţinere a somaclonelor de varză |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD2060G2 (ro) * | 2001-03-28 | 2003-06-30 | Институт Генетики И Физиологии Растений Академии Наук Молдовы | Procedeu de multiplicare vegetativă a levănţicii |
-
1996
- 1996-09-20 MD MD96-0257A patent/MD722G2/ro active IP Right Grant
Non-Patent Citations (2)
| Title |
|---|
| Theoretical and Applied Genetics, vol. 57, ą3, 1980, New York, W.C.Chang, Y.I.Hsing."Plant regeneration through somatic embryogenesis in root-derived callus of ginseng (Panax ginseng C.A.Meyer)", p. 133-135. * |
| Ботанический журнал, т. 53, №7, 1968 г., Наука, Л., Р.Г. Бутенко, И.В. Грушевицкий, Л.И. Слепян."Органогенез и соматический эмбриогенез в культуре тканей женьшеня и других представителей рода Раnax L.", c. 906-911. * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD461Z (ro) * | 2011-06-03 | 2012-08-31 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Procedeu de obţinere a somaclonelor de varză |
Also Published As
| Publication number | Publication date |
|---|---|
| MD722F1 (ro) | 1997-05-31 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG3A | Granted patent for invention | ||
| IF99 | Valid patent on 19990615 |
Free format text: EXPIRES: 20160920 |