MD722G2 - The somatic embryoid production process - Google Patents
The somatic embryoid production processInfo
- Publication number
- MD722G2 MD722G2 MD96-0257A MD960257A MD722G2 MD 722 G2 MD722 G2 MD 722G2 MD 960257 A MD960257 A MD 960257A MD 722 G2 MD722 G2 MD 722G2
- Authority
- MD
- Moldova
- Prior art keywords
- callus
- somatic
- cultivation
- medium
- panax ginseng
- Prior art date
Links
- 230000000392 somatic effect Effects 0.000 title abstract 4
- 238000004519 manufacturing process Methods 0.000 title abstract 3
- 206010020649 Hyperkeratosis Diseases 0.000 abstract 5
- 240000004371 Panax ginseng Species 0.000 abstract 3
- 235000002789 Panax ginseng Nutrition 0.000 abstract 3
- 235000008434 ginseng Nutrition 0.000 abstract 3
- 239000002609 medium Substances 0.000 abstract 3
- 238000012423 maintenance Methods 0.000 abstract 2
- 241000196324 Embryophyta Species 0.000 abstract 1
- 239000013543 active substance Substances 0.000 abstract 1
- 239000012737 fresh medium Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 239000013589 supplement Substances 0.000 abstract 1
- 238000002054 transplantation Methods 0.000 abstract 1
- 230000035899 viability Effects 0.000 abstract 1
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention relates to the agriculture and may be used in biotechnology for the Panax ginseng callus “artificial seeds” production (S.A.Meyer).The summary of the invention consists in the fact, that a somatic embryoid process is proposed, which includes the root explant callus Panax ginseng production and the viability maintenance of it on the Murashige-Skoog medium with the biological active substance supplement by cultivation transplantation on fresh medium each 4-5 weeks, the callus transfer on the medium, which supplementary contains epibrasinolid as the embryogeny stimulant in the concentration of 10-6- 10-5%, the callus cultivation within 40-60 days with a photoperiod of 16 daylight hours and 8 dark hours, the transfer of it on the Murashige-Skoog medium with a macro- and microelement reduced content, on which the somatic embryoids appear after 75-90 days of cultivation.The technical result of the invention consists in the maintenance of the Panax ginseng callus unbounded capacity to produce the somatic embryoids efficient to generate viable plants.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MD96-0257A MD722G2 (en) | 1996-09-20 | 1996-09-20 | The somatic embryoid production process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MD96-0257A MD722G2 (en) | 1996-09-20 | 1996-09-20 | The somatic embryoid production process |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MD722F1 MD722F1 (en) | 1997-05-31 |
| MD722G2 true MD722G2 (en) | 1997-12-31 |
Family
ID=19738901
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MD96-0257A MD722G2 (en) | 1996-09-20 | 1996-09-20 | The somatic embryoid production process |
Country Status (1)
| Country | Link |
|---|---|
| MD (1) | MD722G2 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD461Z (en) * | 2011-06-03 | 2012-08-31 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Process for the production of cabbage somaclones |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD2060G2 (en) * | 2001-03-28 | 2003-06-30 | Институт Генетики И Физиологии Растений Академии Наук Молдовы | Process for lavender vegetative multiplication |
-
1996
- 1996-09-20 MD MD96-0257A patent/MD722G2/en active IP Right Grant
Non-Patent Citations (2)
| Title |
|---|
| Theoretical and Applied Genetics, vol. 57, ą3, 1980, New York, W.C.Chang, Y.I.Hsing."Plant regeneration through somatic embryogenesis in root-derived callus of ginseng (Panax ginseng C.A.Meyer)", p. 133-135. * |
| Ботанический журнал, т. 53, №7, 1968 г., Наука, Л., Р.Г. Бутенко, И.В. Грушевицкий, Л.И. Слепян."Органогенез и соматический эмбриогенез в культуре тканей женьшеня и других представителей рода Раnax L.", c. 906-911. * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD461Z (en) * | 2011-06-03 | 2012-08-31 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Process for the production of cabbage somaclones |
Also Published As
| Publication number | Publication date |
|---|---|
| MD722F1 (en) | 1997-05-31 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG3A | Granted patent for invention | ||
| IF99 | Valid patent on 19990615 |
Free format text: EXPIRES: 20160920 |