MD461Z - Process for the production of cabbage somaclones - Google Patents
Process for the production of cabbage somaclones Download PDFInfo
- Publication number
- MD461Z MD461Z MDS20110099A MDS20110099A MD461Z MD 461 Z MD461 Z MD 461Z MD S20110099 A MDS20110099 A MD S20110099A MD S20110099 A MDS20110099 A MD S20110099A MD 461 Z MD461 Z MD 461Z
- Authority
- MD
- Moldova
- Prior art keywords
- somaclones
- cabbage
- murashige
- followed
- morphogenic
- Prior art date
Links
- 240000007124 Brassica oleracea Species 0.000 title abstract 3
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 title abstract 3
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 title abstract 3
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 3
- 238000004519 manufacturing process Methods 0.000 title abstract 2
- 235000015097 nutrients Nutrition 0.000 abstract 3
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 abstract 2
- 206010020649 Hyperkeratosis Diseases 0.000 abstract 2
- 230000000921 morphogenic effect Effects 0.000 abstract 2
- 229920001817 Agar Polymers 0.000 abstract 1
- 241000701489 Cauliflower mosaic virus Species 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 abstract 1
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 abstract 1
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 abstract 1
- 230000006978 adaptation Effects 0.000 abstract 1
- 239000008272 agar Substances 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 238000011534 incubation Methods 0.000 abstract 1
- 208000015181 infectious disease Diseases 0.000 abstract 1
- 238000011081 inoculation Methods 0.000 abstract 1
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 abstract 1
- 229960001669 kinetin Drugs 0.000 abstract 1
- 239000002689 soil Substances 0.000 abstract 1
- 230000001954 sterilising effect Effects 0.000 abstract 1
- 238000004659 sterilization and disinfection Methods 0.000 abstract 1
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to biotechnology, in particular to a process for the production of cabbage somaclones.The process includes the mechanical infection of plants in the phase of two cotyledonous leaves with the cauliflower mosaic virus, selection of mesophilic tissue explants from the chlorotic areas adjacent sectors, their sterilization, inoculation of explants in the Murashige-Skoog nutrient medium, containing 7 g/l of agar, 0.01 mg/l of kinetin and additionally 0.5 mg/l of 6-benzylaminopurine, 0.5 mg/l of naphthaleneacetic acid, followed by their incubation in the dark at the temperature of 25°C to initiate the formation of morphogenic callus, and then with a photoperiod of 16 hours at the light of 2000 lx and the temperature of 27°C, transfer of morphogenic calluses to fresh nutrient media to obtain somaclones and their subsequent establishment in the Murashige-Skoog nutrient medium, containing additionally 0.5 mg/l of naphthaleneacetic acid, followed by adaptation to ex-vitro conditions and planting in soil of somaclones.The result of the invention is to increase the somaclonal variation of cabbage and the possibility of selecting somaclones with quantitative traits, non-specific for the original variety.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20110099A MD461Z (en) | 2011-06-03 | 2011-06-03 | Process for the production of cabbage somaclones |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20110099A MD461Z (en) | 2011-06-03 | 2011-06-03 | Process for the production of cabbage somaclones |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MD461Y MD461Y (en) | 2012-01-31 |
| MD461Z true MD461Z (en) | 2012-08-31 |
Family
ID=45815355
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MDS20110099A MD461Z (en) | 2011-06-03 | 2011-06-03 | Process for the production of cabbage somaclones |
Country Status (1)
| Country | Link |
|---|---|
| MD (1) | MD461Z (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1323046A1 (en) * | 1984-11-29 | 1987-07-15 | Всесоюзный селекционно-генетический институт | Method of selecting lucerne for resistance to infectious diseases |
| SU1729337A1 (en) * | 1990-04-16 | 1992-04-30 | Всесоюзный Научно-Исследовательский Институт Кукурузы | Method for initial seed-farming of corn hybrids |
| MD722G2 (en) * | 1996-09-20 | 1997-12-31 | Институт Физиологии Растений Академии Наук Республики Молдова | The somatic embryoid production process |
| MD3899C2 (en) * | 2009-02-23 | 2009-12-31 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Process for micropropagation of Echinacea purpurea L. Moench in vitro |
-
2011
- 2011-06-03 MD MDS20110099A patent/MD461Z/en not_active IP Right Cessation
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1323046A1 (en) * | 1984-11-29 | 1987-07-15 | Всесоюзный селекционно-генетический институт | Method of selecting lucerne for resistance to infectious diseases |
| SU1729337A1 (en) * | 1990-04-16 | 1992-04-30 | Всесоюзный Научно-Исследовательский Институт Кукурузы | Method for initial seed-farming of corn hybrids |
| MD722G2 (en) * | 1996-09-20 | 1997-12-31 | Институт Физиологии Растений Академии Наук Республики Молдова | The somatic embryoid production process |
| MD3899C2 (en) * | 2009-02-23 | 2009-12-31 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Process for micropropagation of Echinacea purpurea L. Moench in vitro |
Non-Patent Citations (1)
| Title |
|---|
| În vitro technology for mutation breeding. A technical document issued by the International Atomic Energy Agency, IAEA-TECDOC 392, Vienna, 1986, p. 9-10, 12-14, 34, 47 * |
Also Published As
| Publication number | Publication date |
|---|---|
| MD461Y (en) | 2012-01-31 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| KA4Y | Short-term patent lapsed due to non-payment of fees (with right of restoration) |