MD461Y - Procedeu de obţinere a somaclonelor de varză - Google Patents
Procedeu de obţinere a somaclonelor de varză Download PDFInfo
- Publication number
- MD461Y MD461Y MDS20110099A MDS20110099A MD461Y MD 461 Y MD461 Y MD 461Y MD S20110099 A MDS20110099 A MD S20110099A MD S20110099 A MDS20110099 A MD S20110099A MD 461 Y MD461 Y MD 461Y
- Authority
- MD
- Moldova
- Prior art keywords
- somaclones
- cabbage
- murashige
- followed
- morphogenic
- Prior art date
Links
- 240000007124 Brassica oleracea Species 0.000 title abstract 3
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 title abstract 3
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 title abstract 3
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 3
- 238000004519 manufacturing process Methods 0.000 title abstract 2
- 235000015097 nutrients Nutrition 0.000 abstract 3
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 abstract 2
- 206010020649 Hyperkeratosis Diseases 0.000 abstract 2
- 230000000921 morphogenic effect Effects 0.000 abstract 2
- 229920001817 Agar Polymers 0.000 abstract 1
- 241000701489 Cauliflower mosaic virus Species 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 abstract 1
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 abstract 1
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 abstract 1
- 230000006978 adaptation Effects 0.000 abstract 1
- 239000008272 agar Substances 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 238000011534 incubation Methods 0.000 abstract 1
- 208000015181 infectious disease Diseases 0.000 abstract 1
- 238000011081 inoculation Methods 0.000 abstract 1
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 abstract 1
- 229960001669 kinetin Drugs 0.000 abstract 1
- 239000002689 soil Substances 0.000 abstract 1
- 230000001954 sterilising effect Effects 0.000 abstract 1
- 238000004659 sterilization and disinfection Methods 0.000 abstract 1
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Invenţia se referă la biotehnologie, în particular la un procedeu de obţinere a somaclonelor de varză.Procedeul include infectarea mecanică a plantelor în faza de două frunzuliţe cotiledonate cu virusul mozaicul conopidei, colectarea explantelor de ţesut mezofil din sectoarele învecinate zonelor clorotice, sterilizarea lor, inocularea explantelor pe mediul nutritiv Murashige şi Skoog, ce conţine agar 7 g/l, chinetină 0,01 mg/l şi suplimentar 6-benzilaminopurină 0,5 mg/l, acid naftilacetic 0,5 mg/l, cu incubarea ulterioară a lor la întuneric la temperatura de 25°C pentru iniţierea formării calusului morfogen, apoi cu o fotoperioadă de 16 ore la iluminare de 2000 lx şi temperatura de 27°C, transferarea calusurilor morfogene pe medii nutritive proaspete cu obţinerea somaclonelor şi înrădăcinarea ulterioară a lor pe mediul nutritiv Murashige şi Skoog, ce conţine suplimentar acid naftilacetic 0,5 mg/l cu adaptarea ulterioară la condiţii ex-vitro şi plantarea în sol a somaclonelor.Rezultatul invenţiei constă în sporirea variabilităţii somaclonale la varză şi în posibilitatea selecţiei somaclonelor care posedă caractere cantitative, nespecifice pentru soiul iniţial.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20110099A MD461Z (ro) | 2011-06-03 | 2011-06-03 | Procedeu de obţinere a somaclonelor de varză |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20110099A MD461Z (ro) | 2011-06-03 | 2011-06-03 | Procedeu de obţinere a somaclonelor de varză |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MD461Y true MD461Y (ro) | 2012-01-31 |
| MD461Z MD461Z (ro) | 2012-08-31 |
Family
ID=45815355
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MDS20110099A MD461Z (ro) | 2011-06-03 | 2011-06-03 | Procedeu de obţinere a somaclonelor de varză |
Country Status (1)
| Country | Link |
|---|---|
| MD (1) | MD461Z (ro) |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1323046A1 (ru) * | 1984-11-29 | 1987-07-15 | Всесоюзный селекционно-генетический институт | Способ селекции люцерны на устойчивость к инфекционным заболевани м |
| SU1729337A1 (ru) * | 1990-04-16 | 1992-04-30 | Всесоюзный Научно-Исследовательский Институт Кукурузы | Способ первичного семеноводства гибридов кукурузы |
| MD722G2 (ro) * | 1996-09-20 | 1997-12-31 | Институт Физиологии Растений Академии Наук Республики Молдова | Procedeu de obţinere a embriozilor somatici |
| MD3899C2 (ro) * | 2009-02-23 | 2009-12-31 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Procedeu de micropropagare a Echinacea purpurea L. Moench in vitro |
-
2011
- 2011-06-03 MD MDS20110099A patent/MD461Z/ro not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| MD461Z (ro) | 2012-08-31 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| KA4Y | Short-term patent lapsed due to non-payment of fees (with right of restoration) |