Институт Микробиологии И Биотехнологии Академии Наук Молдовы
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Application filed by Институт Микробиологии И Биотехнологии Академии Наук МолдовыfiledCriticalИнститут Микробиологии И Биотехнологии Академии Наук Молдовы
Priority to MDS20180110ApriorityCriticalpatent/MD1380Z/en
Publication of MD1380YpublicationCriticalpatent/MD1380Y/en
Publication of MD1380ZpublicationCriticalpatent/MD1380Z/en
Micro-Organisms Or Cultivation Processes Thereof
(AREA)
Abstract
The invention relates to biotechnology, in particular to a process for preserving the Saccharomyces cerevisiae CNMN-Y-20 yeast strain, which can be used for long-term storage of microorganisms and their use as sources of biologically active substances.The process, according to the invention, consists in cultivating the Saccharomyces cerevisiae CNMN-Y-20 yeast strain on a nutrient medium for 72 hours at a temperature of 26…28°C, suspending the culture to a titer of 105…106 ml-1 in a medium consisting of skim milk and 5…10% vol. 65% water-ethanol solution, comprising 5 mg/ml of extract, obtained from spirulina biomass, rapid freezing of suspension at a temperature of -20°C, lyophilization and storage at a temperature of 4°C.The technical result of the invention consists in increasing the protein content in the Saccharomyces cerevisiae CNMN-Y-20 yeast biomass after one year of storage in a lyophilized state by 20.70…40.48%.
MDS20180110A2018-11-272018-11-27Process for preserving the Saccharomyces cerevisiae CNMN-Y-20 yeast strain
MD1380Z
(en)
A feed additive composition comprising a mixture of lactic acid bacteria cultured with Industrial medium for shrimp and manufacturing method of thereof