CN102719386B - Bacterium and fungus strain storage culture medium - Google Patents
Bacterium and fungus strain storage culture medium Download PDFInfo
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- CN102719386B CN102719386B CN 201210242292 CN201210242292A CN102719386B CN 102719386 B CN102719386 B CN 102719386B CN 201210242292 CN201210242292 CN 201210242292 CN 201210242292 A CN201210242292 A CN 201210242292A CN 102719386 B CN102719386 B CN 102719386B
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- glycerine
- strain storage
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Abstract
The invention relates to a bacterium and fungus strain storage culture medium comprising the following components: tryptone, sodium chloride, agar, yeas extract and glycerol. The tryptone is used for providing a microorganism nitrogen source; the sodium chloride is used for maintaining a uniform osmotic pressure; the agar is used as a supporting component in the culture medium; the yeas extract is rich in proteins, amino acids, peptides, nucleotides and vitamins B and can be used for replenishing various vitamins, amino acids and growth factors for the growth of microorganisms; and the glycerol is used as a carbon source and can be used for playing roles of preventing freeze and preserving moisture. The bacterium and fungus strain storage culture medium is simple and convenient in operation of strain storage, long in storage time, high in stability and capable of increasing the survival rate and the reproductive rate of the strains.
Description
Technical field
The present invention relates to preserve the technical field of bacterial classification, specifically a kind of bacterium, fungi strain are preserved substratum.
Background technology
At present bacterial classification store method commonly used has freeze-drying, semisolid medium method and simple glycerine preservation method etc.These methods have certain defective, and as freeze-drying, the preparation process complexity needs appointed condition preferably again; The semisolid medium preservation method can not be preserved bacterial classification for a long time; Simple its glycerine of glycerine preservation method is just preserved moisture for antifreeze, and some special bacterial strain survival rate in glycerine stoste is low, and the shelf time is short, and toxicity descends.
Summary of the invention
The present invention is directed to the prior art deficiency, provide a kind of preservation bacterial classification easy and simple to handle, the shelf time is long, and the bacterium of good stability, fungi strain are preserved substratum.
Technical scheme of the present invention is: a kind of bacterium, fungi strain preserve substratum, it is characterized in that it is made up of the following raw materials in weight proportioning: Tryptones 0.8-1.2g, sodium-chlor 0.8-1.2g, agar 0.2-0.5g, yeast extract paste 0.3-0.6g, glycerine 15ml, distilled water 85ml.
Preparation method's step is as follows: A, add Tryptones, sodium-chlor, agar, yeast extract paste, glycerine in the distilled water in proportion; B, heating for dissolving are transferred PH7.5; C, be divided in the EP pipe every pipe 0.5-1ml, D, 8 pounds of sterilizations in 20 minutes ,-20 ℃ of preservations.
Tryptones among the present invention provides the microorganism nitrogenous source; Sodium-chlor is kept balanced osmotic pressure; Agar is the support composition in the substratum; Yeast extract paste rich in proteins, amino acid, peptide class, Nucleotide, vitamin B group can replenish nitrogenous source and various VITAMIN, amino acid and somatomedin that microorganism growth is provided; Glycerine has antifreeze and moisture-keeping function as carbon source.
The present invention has the following advantages: preparation is simple, easily, and cost is low, and is easy to use, and it is easy and simple to handle to preserve bacterial classification, and the shelf time is long, and good stability improves survival rate and the breeding potential of bacterial classification greatly.
Embodiment
Embodiment 1: a kind of bacterium, fungi strain are preserved substratum, prepare according to following column data and described step: Tryptones 0.8-1.2g, sodium-chlor 0.8-1.2g, agar 0.2-0.5g, yeast extract paste 0.3-0.6g, glycerine 15ml, distilled water 85ml.
Preparation method's step is as follows: A, add Tryptones, sodium-chlor, agar, yeast extract paste, glycerine in the distilled water in proportion; B, heating for dissolving are transferred PH7.5; C, be divided in the EP pipe every pipe 0.5-1ml, D, 8 pounds of sterilizations in 20 minutes ,-20 ℃ of preservations.
Embodiment 2: a kind of bacterium, fungi strain are preserved substratum, prepare according to following column data and described step: Tryptones 1.0g, sodium-chlor 1.0g, agar 0.3g, yeast extract paste 0.5g, glycerine 15ml, distilled water 85ml.
Preparation method's step is as follows: A, remove Tryptones 1.0g, and sodium-chlor 1.0g, agar 0.3g, yeast extract paste 0.5g, glycerine 15ml add in the distilled water; B, heating for dissolving are transferred PH7.5; C, be divided in the EP pipe every pipe 0.5-1ml, D, 8 pounds of sterilizations in 20 minutes ,-20 ℃ of preservations.
Embodiment 3: a kind of bacterium, fungi strain are preserved substratum, prepare according to following column data and described step: Tryptones 0.9g, sodium-chlor 0.9g, agar 0.4g, yeast extract paste 0.6g, glycerine 15ml, distilled water 85ml.
Preparation method's step is as follows: A, get Tryptones 0.9g, and sodium-chlor 0.9g, agar 0.4g, yeast extract paste 0.6g, glycerine 15ml add in the distilled water; B, heating for dissolving are transferred PH7.5; C, be divided in the EP pipe every pipe 0.5-1ml, D, 8 pounds of sterilizations in 20 minutes ,-20 ℃ of preservations.
Embodiment 4: a kind of bacterium, fungi strain are preserved substratum, prepare according to following column data and described step: Tryptones 1.2g, sodium-chlor 1.2g, agar 0.5g, yeast extract paste 0.4g, glycerine 15ml, distilled water 85ml.
Preparation method's step is as follows: A, get Tryptones 1.2g, and sodium-chlor 1.2g, agar 0.5g, yeast extract paste 0.4g, glycerine 15ml add in the distilled water; B, heating for dissolving are transferred PH7.5; C, be divided in the EP pipe every pipe 0.5-1ml, D, 8 pounds of sterilizations in 20 minutes ,-20 ℃ of preservations.
Effect is judged:
Bacterial classification to be preserved scraped with transfering loop get 4-5 ring, place the EP pipe that above-mentioned substratum is housed ,-20 ℃ of refrigerators are preserved.The bacterial classification of preserving has bacterial classifications such as streptococcus aureus, escherichia coli, Pseudomonas aeruginosa, Salmonella typhi and Candida albicans, show through the saving result more than 5 years, the morphological structure of bacterium, dyeing property, biochemical reaction, antigenic structure etc. all conforms to reference culture.
Claims (2)
1. a bacterium, fungi strain are preserved substratum, it is characterized in that it is made up of the following raw materials in weight proportioning: Tryptones 0.8-1.2g, sodium-chlor 0.8-1.2g, agar 0.2-0.5g, yeast extract paste 0.3-0.6g, glycerine 15ml, distilled water 85ml.
2. bacterium as claimed in claim 1, fungi strain preserve the preparation method of substratum, it is characterized in that step is as follows: A, add Tryptones, sodium-chlor, yeast extract paste, agar, glycerine in the distilled water in proportion; PH7.5 is transferred in B, heating fusion; C, be divided in the EP pipe every pipe 0.5-1ml, D, 8 pounds of sterilizations in 20 minutes ,-20 ℃ of preservations.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210242292 CN102719386B (en) | 2012-07-01 | 2012-07-01 | Bacterium and fungus strain storage culture medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210242292 CN102719386B (en) | 2012-07-01 | 2012-07-01 | Bacterium and fungus strain storage culture medium |
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| Publication Number | Publication Date |
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| CN102719386A CN102719386A (en) | 2012-10-10 |
| CN102719386B true CN102719386B (en) | 2013-08-28 |
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| CN 201210242292 Expired - Fee Related CN102719386B (en) | 2012-07-01 | 2012-07-01 | Bacterium and fungus strain storage culture medium |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103642886A (en) * | 2013-11-18 | 2014-03-19 | 宁夏泰瑞制药股份有限公司 | Medium for producing demethylchlortetracycline by fermenting streptomyces aureus and culturing method |
| CN103718830B (en) * | 2013-12-27 | 2015-08-19 | 湖南农业大学 | A kind of factorial praluction pleurotus eryngii quel strains cryopreservation method |
| CN104498380A (en) * | 2014-08-04 | 2015-04-08 | 湖北文理学院 | Staphylococcus ATCC25923 proliferation promoter and application method thereof |
| CN105602849A (en) * | 2014-09-05 | 2016-05-25 | 杭州致远医学检验所有限公司 | Special sample tissue storage and transportation culture medium for Helicobacter pylori separation culture |
| CN104673685B (en) * | 2015-03-16 | 2018-11-16 | 广西中医药大学 | A kind of isolation medium of Radix zanthoxyli endogenetic fungus |
| CN108949572A (en) * | 2018-08-21 | 2018-12-07 | 卓源健康科技有限公司 | A kind of caprophyl freezen protective liquid and its store method |
| CN112159786B (en) * | 2020-11-04 | 2022-03-01 | 河北科技大学 | Cr (VI) reducing strain C6, and culture condition and application thereof |
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| CN1096819A (en) * | 1993-06-23 | 1994-12-28 | 铜陵市卫生防疫站 | A kind of long-acting preservative jejunum campylobacter colony culture medium and preparation method |
| CN102465097A (en) * | 2010-11-10 | 2012-05-23 | 中国科学院生态环境研究中心 | Method for long-term preservation and rapid recovery of luminous bacterium strain |
| CN102174435A (en) * | 2011-01-21 | 2011-09-07 | 贵州大学 | Bacillus subtilis strain and application thereof |
| CN102433373B (en) * | 2011-12-14 | 2013-10-09 | 浙江省农业科学院 | Salmonella characteristic chromogenic liquid nutrient medium, preparation method thereof and rapid detection method of salmonella |
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