Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inst De Microbiologie Si BiotehnologiefiledCriticalInst De Microbiologie Si Biotehnologie
Priority to MDS20180109ApriorityCriticalpatent/MD1379Z/en
Publication of MD1379YpublicationCriticalpatent/MD1379Y/en
Publication of MD1379ZpublicationCriticalpatent/MD1379Z/en
Micro-Organisms Or Cultivation Processes Thereof
(AREA)
Abstract
The invention relates to biotechnology, in particular to a process for preserving the Saccharomyces cerevisiae CNMN-Y-20 yeast strain, which can be used for long-term storage of microorganisms and their use as sources of biologically active substances.The process, according to the invention, consists in cultivating the Saccharomyces cerevisiae CNMN-Y-20 yeast strain on a nutrient medium for 72 hours at a temperature of 26…28°C, suspending the culture in a medium consisting of skim milk and 5…10% vol. solution comprising 10 mg/ml of extract of sulfated polysaccharides, obtained from spirulina biomass, to a titer of 105…106 ml-1, rapid freezing at a temperature of -20°C, lyophilization and storage at a temperature of 4°C.The technical result of the invention consists in increasing the protein content in the Saccharomyces cerevisiae CNMN-Y-20 yeast biomass after one year of storage in a lyophilized state by 25.32…26.62%.
MDS20180109A2018-11-272018-11-27Process for preserving the Saccharomyces cerevisiae CNMN-Y-20 yeast strain
MD1379Z
(en)
A feed additive composition comprising a mixture of lactic acid bacteria cultured with Industrial medium for shrimp and manufacturing method of thereof