Институт Микробиологии И Биотехнологии Академии Наук Молдовы
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Application filed by Институт Микробиологии И Биотехнологии Академии Наук МолдовыfiledCriticalИнститут Микробиологии И Биотехнологии Академии Наук Молдовы
Priority to MDS20180111ApriorityCriticalpatent/MD1396Z/en
Publication of MD1396YpublicationCriticalpatent/MD1396Y/en
Publication of MD1396ZpublicationCriticalpatent/MD1396Z/en
Medicines Containing Material From Animals Or Micro-Organisms
(AREA)
Micro-Organisms Or Cultivation Processes Thereof
(AREA)
Abstract
The invention relates to biotechnology, in particular to a process for preserving the Saccharomyces cerevisiae CNMN-Y-21 yeast strain, which can be used for long-term storage of microorganisms and their use as sources of biologically active substances.The process, according to the invention, comprises cultivation of Saccharomyces cerevisiae CNMN-Y-21 yeast strain on a nutrient medium for 72 hours at a temperature of 26-28°C, suspension of the culture to a titer of 105-106 ml-1 in a medium consisting of skim milk and a 5-10% vol. solution comprising 10 mg/ml of extract, obtained from spirulina biomass by sequential extraction with 20-30% alcoholic solution and 0.45% NaOH solution, rapid freezing of the suspension at a temperature of -20°C, lyophilization and storage at a temperature of 4°C.The technical result of the invention consists in increasing the protein and carbohydrate content in the Saccharomyces cerevisiae CNMN-Y-21 yeast biomassafter one year of storage in a lyophilized state by 35.31-37.03% and 35.62-39.56%, respectively.
MDS20180111A2018-11-272018-11-27Process for preserving the Saccharomyces cerevisiae CNMN-Y-21 yeast strain
MD1396Z
(en)
Effect of Structural Components of Modified Gel Carrier on Enzymatic Properties of Immobilized Industrial Strain of Lactococcus lactis after Low-Temperature Storage