MD1396Z - Process for preserving the Saccharomyces cerevisiae CNMN-Y-21 yeast strain - Google Patents

Process for preserving the Saccharomyces cerevisiae CNMN-Y-21 yeast strain

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Publication number
MD1396Z
MD1396Z MDS20180111A MDS20180111A MD1396Z MD 1396 Z MD1396 Z MD 1396Z MD S20180111 A MDS20180111 A MD S20180111A MD S20180111 A MDS20180111 A MD S20180111A MD 1396 Z MD1396 Z MD 1396Z
Authority
MD
Moldova
Prior art keywords
cnmn
saccharomyces cerevisiae
yeast strain
temperature
preserving
Prior art date
Application number
MDS20180111A
Other languages
Romanian (ro)
Russian (ru)
Inventor
Людмила БАТЫР
Светлана ДЖУР
Валерина СЛАНИНА
Валериу РУДИК
Original Assignee
Институт Микробиологии И Биотехнологии Академии Наук Молдовы
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Институт Микробиологии И Биотехнологии Академии Наук Молдовы filed Critical Институт Микробиологии И Биотехнологии Академии Наук Молдовы
Priority to MDS20180111A priority Critical patent/MD1396Z/en
Publication of MD1396Y publication Critical patent/MD1396Y/en
Publication of MD1396Z publication Critical patent/MD1396Z/en

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  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention relates to biotechnology, in particular to a process for preserving the Saccharomyces cerevisiae CNMN-Y-21 yeast strain, which can be used for long-term storage of microorganisms and their use as sources of biologically active substances.The process, according to the invention, comprises cultivation of Saccharomyces cerevisiae CNMN-Y-21 yeast strain on a nutrient medium for 72 hours at a temperature of 26-28°C, suspension of the culture to a titer of 105-106 ml-1 in a medium consisting of skim milk and a 5-10% vol. solution comprising 10 mg/ml of extract, obtained from spirulina biomass by sequential extraction with 20-30% alcoholic solution and 0.45% NaOH solution, rapid freezing of the suspension at a temperature of -20°C, lyophilization and storage at a temperature of 4°C.The technical result of the invention consists in increasing the protein and carbohydrate content in the Saccharomyces cerevisiae CNMN-Y-21 yeast biomassafter one year of storage in a lyophilized state by 35.31-37.03% and 35.62-39.56%, respectively.
MDS20180111A 2018-11-27 2018-11-27 Process for preserving the Saccharomyces cerevisiae CNMN-Y-21 yeast strain MD1396Z (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
MDS20180111A MD1396Z (en) 2018-11-27 2018-11-27 Process for preserving the Saccharomyces cerevisiae CNMN-Y-21 yeast strain

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
MDS20180111A MD1396Z (en) 2018-11-27 2018-11-27 Process for preserving the Saccharomyces cerevisiae CNMN-Y-21 yeast strain

Publications (2)

Publication Number Publication Date
MD1396Y MD1396Y (en) 2019-11-30
MD1396Z true MD1396Z (en) 2020-06-30

Family

ID=68732609

Family Applications (1)

Application Number Title Priority Date Filing Date
MDS20180111A MD1396Z (en) 2018-11-27 2018-11-27 Process for preserving the Saccharomyces cerevisiae CNMN-Y-21 yeast strain

Country Status (1)

Country Link
MD (1) MD1396Z (en)

Also Published As

Publication number Publication date
MD1396Y (en) 2019-11-30

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