MD1396Y - Process for preserving the Saccharomyces cerevisiae CNMN-Y-21 yeast strain - Google Patents
Process for preserving the Saccharomyces cerevisiae CNMN-Y-21 yeast strainInfo
- Publication number
- MD1396Y MD1396Y MDS20180111A MD1396Y MD 1396 Y MD1396 Y MD 1396Y MD S20180111 A MDS20180111 A MD S20180111A MD 1396 Y MD1396 Y MD 1396Y
- Authority
- MD
- Moldova
- Prior art keywords
- cnmn
- saccharomyces cerevisiae
- yeast strain
- temperature
- preserving
- Prior art date
Links
- 240000004808 Saccharomyces cerevisiae Species 0.000 title abstract 8
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 title abstract 8
- 238000000034 method Methods 0.000 title abstract 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 abstract 3
- 239000000725 suspension Substances 0.000 abstract 2
- 240000002900 Arthrospira platensis Species 0.000 abstract 1
- 235000016425 Arthrospira platensis Nutrition 0.000 abstract 1
- 239000002028 Biomass Substances 0.000 abstract 1
- 239000013543 active substance Substances 0.000 abstract 1
- 230000001476 alcoholic effect Effects 0.000 abstract 1
- 150000001720 carbohydrates Chemical class 0.000 abstract 1
- 238000000605 extraction Methods 0.000 abstract 1
- 238000004108 freeze drying Methods 0.000 abstract 1
- 238000007710 freezing Methods 0.000 abstract 1
- 230000008014 freezing Effects 0.000 abstract 1
- 230000007774 longterm Effects 0.000 abstract 1
- 244000005700 microbiome Species 0.000 abstract 1
- 235000015097 nutrients Nutrition 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 235000020183 skimmed milk Nutrition 0.000 abstract 1
- 229940082787 spirulina Drugs 0.000 abstract 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention relates to biotechnology, in particular to a process for preserving the Saccharomyces cerevisiae CNMN-Y-21 yeast strain, which can be used for long-term storage of microorganisms and their use as sources of biologically active substances.The process, according to the invention, comprises cultivation of Saccharomyces cerevisiae CNMN-Y-21 yeast strain on a nutrient medium for 72 hours at a temperature of 26-28°C, suspension of the culture to a titer of 105-106 ml-1 in a medium consisting of skim milk and a 5-10% vol. solution comprising 10 mg/ml of extract, obtained from spirulina biomass by sequential extraction with 20-30% alcoholic solution and 0.45% NaOH solution, rapid freezing of the suspension at a temperature of -20°C, lyophilization and storage at a temperature of 4°C.The technical result of the invention consists in increasing the protein and carbohydrate content in the Saccharomyces cerevisiae CNMN-Y-21 yeast biomassafter one year of storage in a lyophilized state by 35.31-37.03% and 35.62-39.56%, respectively.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20180111A MD1396Z (en) | 2018-11-27 | 2018-11-27 | Process for preserving the Saccharomyces cerevisiae CNMN-Y-21 yeast strain |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20180111A MD1396Z (en) | 2018-11-27 | 2018-11-27 | Process for preserving the Saccharomyces cerevisiae CNMN-Y-21 yeast strain |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MD1396Y true MD1396Y (en) | 2019-11-30 |
| MD1396Z MD1396Z (en) | 2020-06-30 |
Family
ID=68732609
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MDS20180111A MD1396Z (en) | 2018-11-27 | 2018-11-27 | Process for preserving the Saccharomyces cerevisiae CNMN-Y-21 yeast strain |
Country Status (1)
| Country | Link |
|---|---|
| MD (1) | MD1396Z (en) |
-
2018
- 2018-11-27 MD MDS20180111A patent/MD1396Z/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| MD1396Z (en) | 2020-06-30 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG9Y | Short term patent issued | ||
| KA4Y | Short-term patent lapsed due to non-payment of fees (with right of restoration) |