LU505383B1 - Microfluidic chip for screening migration of mesenchymal stem cell (msc) to tumor, and preparation method and use method thereof - Google Patents

Microfluidic chip for screening migration of mesenchymal stem cell (msc) to tumor, and preparation method and use method thereof Download PDF

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Publication number
LU505383B1
LU505383B1 LU505383A LU505383A LU505383B1 LU 505383 B1 LU505383 B1 LU 505383B1 LU 505383 A LU505383 A LU 505383A LU 505383 A LU505383 A LU 505383A LU 505383 B1 LU505383 B1 LU 505383B1
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Luxembourg
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cell
channel
microfluidic chip
migration
matrigel
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LU505383A
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German (de)
English (en)
Inventor
Luoran Shang
Xin Shou
Yizuo Song
Xueqiong Zhu
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The Second Affiliated Hospital And Yuying Childrens Hospital Of Wenzhou Medical Univ
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0697Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/16Microfluidic devices; Capillary tubes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0652Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662Stem cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0693Tumour cells; Cancer cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00Supports or coatings for cell culture, characterised by material
    • C12N2533/50Proteins
    • C12N2533/54Collagen; Gelatin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00Supports or coatings for cell culture, characterised by material
    • C12N2533/90Substrates of biological origin, e.g. extracellular matrix, decellularised tissue
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2535/00Supports or coatings for cell culture characterised by topography
    • C12N2535/10Patterned coating

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Sustainable Development (AREA)
  • Dispersion Chemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Rheumatology (AREA)
  • Clinical Laboratory Science (AREA)
  • Oncology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
LU505383A 2022-10-18 2022-12-22 Microfluidic chip for screening migration of mesenchymal stem cell (msc) to tumor, and preparation method and use method thereof LU505383B1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202211270154.3A CN117264763A (zh) 2022-10-18 2022-10-18 一种用于间充质干细胞迁移筛选的肿瘤微流控芯片及其制备和应用方法

Publications (1)

Publication Number Publication Date
LU505383B1 true LU505383B1 (en) 2024-04-26

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Application Number Title Priority Date Filing Date
LU505383A LU505383B1 (en) 2022-10-18 2022-12-22 Microfluidic chip for screening migration of mesenchymal stem cell (msc) to tumor, and preparation method and use method thereof

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CN (1) CN117264763A (zh)
LU (1) LU505383B1 (zh)
WO (1) WO2024082430A1 (zh)

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2274438A4 (en) * 2008-04-08 2013-09-11 Massachusetts Inst Technology THREE-DIMENSIONAL MICROFLUIDIC PLATFORMS AND METHODS OF USING THE SAME
CN103627635B (zh) * 2013-11-18 2015-05-20 辽宁中医药大学 一种用于细胞迁移和侵袭实验的多功能微流控芯片
CN105713835B (zh) * 2014-12-05 2018-11-09 中国科学院大连化学物理研究所 一种基于微流控芯片的多功能区域细胞三维共培养方法
CN106566863B (zh) * 2015-10-10 2020-11-20 中国科学院大连化学物理研究所 一种基于微流控芯片的细胞双向侵袭监测方法
CN107955783B (zh) * 2016-10-14 2021-11-30 中国科学院大连化学物理研究所 一种基于微流控芯片体外糖尿病肾小球模型的构建方法
CN107955784B (zh) * 2016-10-14 2021-10-15 中国科学院大连化学物理研究所 一种基于微流控芯片技术的三维细胞球迁移监测方法
CN106544271A (zh) * 2016-12-07 2017-03-29 清华大学深圳研究生院 一种研究肿瘤侵袭血管的多细胞3d共培养装置和方法
CN114480122A (zh) * 2022-01-24 2022-05-13 中国人民解放军海军军医大学 一种基于微流控芯片的血脑屏障与脑胶质瘤共培养模型的建立方法及应用

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CN117264763A (zh) 2023-12-22
WO2024082430A1 (zh) 2024-04-25

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