LU503558B1 - A new use of Hermetia illucens L., its Cantheconidae furcellata (walff) food source, Cantheconidae furcellata (walff) artificial feeding method and industrial production method thereof - Google Patents
A new use of Hermetia illucens L., its Cantheconidae furcellata (walff) food source, Cantheconidae furcellata (walff) artificial feeding method and industrial production method thereof Download PDFInfo
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- LU503558B1 LU503558B1 LU503558A LU503558A LU503558B1 LU 503558 B1 LU503558 B1 LU 503558B1 LU 503558 A LU503558 A LU 503558A LU 503558 A LU503558 A LU 503558A LU 503558 B1 LU503558 B1 LU 503558B1
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- 235000013305 food Nutrition 0.000 title claims abstract description 109
- 241000709785 Hermetia illucens Species 0.000 title claims abstract description 71
- 238000000034 method Methods 0.000 title claims abstract description 50
- 238000009776 industrial production Methods 0.000 title claims abstract description 29
- 241000238631 Hexapoda Species 0.000 claims abstract description 79
- 238000009395 breeding Methods 0.000 claims abstract description 40
- 230000001488 breeding effect Effects 0.000 claims abstract description 39
- 230000016087 ovulation Effects 0.000 claims abstract description 27
- 235000013601 eggs Nutrition 0.000 claims description 98
- 241000255789 Bombyx mori Species 0.000 claims description 44
- 238000004806 packaging method and process Methods 0.000 claims description 41
- 238000004519 manufacturing process Methods 0.000 claims description 35
- 238000003860 storage Methods 0.000 claims description 31
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 30
- 230000001418 larval effect Effects 0.000 claims description 27
- 238000005485 electric heating Methods 0.000 claims description 24
- 241000905789 Lactobacillus larvae Species 0.000 claims description 23
- 241000894007 species Species 0.000 claims description 23
- 238000005070 sampling Methods 0.000 claims description 20
- 229920000742 Cotton Polymers 0.000 claims description 18
- 238000012216 screening Methods 0.000 claims description 18
- 239000010902 straw Substances 0.000 claims description 15
- 230000013011 mating Effects 0.000 claims description 12
- 235000012907 honey Nutrition 0.000 claims description 11
- 238000007689 inspection Methods 0.000 claims description 9
- 240000008042 Zea mays Species 0.000 claims description 6
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 6
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 6
- 235000005822 corn Nutrition 0.000 claims description 6
- 235000013312 flour Nutrition 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims description 6
- 235000015099 wheat brans Nutrition 0.000 claims description 6
- 230000032669 eclosion Effects 0.000 claims description 5
- 241000196324 Embryophyta Species 0.000 claims description 4
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims description 4
- 238000010923 batch production Methods 0.000 claims description 4
- 230000029052 metamorphosis Effects 0.000 claims description 4
- 230000002093 peripheral effect Effects 0.000 claims description 3
- 244000061176 Nicotiana tabacum Species 0.000 claims 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
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- Biodiversity & Conservation Biology (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Animal Behavior & Ethology (AREA)
- Birds (AREA)
- Insects & Arthropods (AREA)
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Abstract
The present invention " a new use of Hermetia illucens L., its Cantheconidae furcellata (walff) food source, Cantheconidae furcellata (walff) artificial feeding method and industrial production method thereof ", belongs to a field of breeding technology of natural enemy insect against agricultural and forestry pests. The invention provides a new use of Hermetia illucens L., and based on the new use, provides a Cantheconidae furcellata (walff) food source, an Cantheconidae furcellata (walff) artificial feeding method and an Cantheconidae furcellata (walff) industrial production method. The new use, new food source and new method of the invention can effectively optimized the spawning rate, ovulation rate, breeding cycle, uniformity and activity of Cantheconidae furcellata (walff).
Description
Our Ref: BNT240706LUCN 1
LU503558
SPECIFICATION
A new use of Hermetia illucens L., its Cantheconidae furcellata (walff) food source, Cantheconidae furcellata (walff) artificial feeding method and industrial production method thereof
The present invention belongs to a field of breeding technology of natural enemy insect of agricultural and forestry pests, specifically relates to a new use of
Hermetia illucens L., its Cantheconidae furcellata (walff) food source,
Cantheconidae furcellata (walff) artificial feeding method and industrial production method thereof.
Lepidoptera insects mainly include two types of insects, moths and butterflies, most of which are pests. Lepidoptera pests are a large class of pests in production of agricultural and forestry crops, of which have characteristics of harming many crop species, and a wide range of distribution; wherein the main lepidoptera pests are Spodoptera litura Fabricius, Mythimna separata (Walker),
Cnaphalocrocis medinalis, Agrotis ypsilon, Helicoverpa armigera (Hubner) ,
Pieris rapae, Plutella xylostella (Linnaeus), Spodoptera frugiperda, etc., and these main lepidopteran pests seriously affect yield and quality of agricultural and forestry crops. Currently the main means of control of lepidoptera pests is spraying chemical pesticides, As eliminating lepidopteran pests, chemical pesticides simultaneously cause pollution of agricultural and forestry crop products, affect food safety, and destroy the ecological environment, result in reduction of biodiversity. Meanwhile, use of a large number of chemical pesticides has strengthened resistance of lepidopteran pests year by year, and then control effect of some lepidopteran pests has been greatly reduced, and use amount of chemical pesticides has to be increased in production, which has caused a vicious circle.
With the advancement on construction of agricultural and forestry ecological civilization, trend of pest natural enemy prevention and control technology in
China has developed to a certain extent in recent years. Among them, the
Our Ref: BNT240706LUCN 2
LU503558 biological control technology of lepidopteran pests is one of the most effective ways to control lepidoptera pests. Cantheconidae furcellata (walff) is one of the dominant natural enemies against most lepidoptera pests. Cantheconidae furcellata (walff) belongs to Hemiptera, Pentatomidae stinkbug, Picromerus lewisi subfamily, Cantheconidea concinna (Walker) genus, and is widely distributed in tropical as well as subtropical regions, distributed in many provinces in southern
China. It is an important predatory natural enemy insect, especially has a good control effect against some lepidoptera pests, and can prey on eggs, larvae, pupae and adults of various phytophagous pests such as Coleoptera,
Hymenoptera and Hemiptera. This natural enemy insect has a large range of activities, a wide range of variety of predatory pests, strong predatory ability, and is known as the "insecticidal king", and has great development value in the biological control technology of lepidopteran pests.
At present, the existing biotechnology for prevention and control against
Lepidoptera insects by Cantheconidae furcellata (walff) is still basically in research and demonstration stage, and there are some problems like that, imperfect breeding facilities and equipment for Cantheconidae furcellata (walff), difficulties in collecting native indigenous species; some key breeding technologies have not broken through; low spawning rate; low ovulation rate; severe mutual predation; long breeding cycle; uniformity of the bred
Cantheconidae furcellata (walff) is poor; Insufficient activity; breeding requires large labor and high energy consumption. Therefore, currently there is an urgent need for a large-scale, efficient and stable Cantheconidae furcellata (walff) production method, that is, an industrial production method of Cantheconidae furcellata (walff).
In order to solve the problems that imperfect breeding facilities and equipment for
Cantheconidae furcellata (walff), difficulties in collecting native indigenous species; low spawning rate; low ovulation rate; severe mutual predation; long breeding cycle; uniformity of the bred Cantheconidae furcellata (walff) is poor, insufficient activity; breeding requires large labor and high energy consumption, etc., in prior art, the object of the present invention is to provide a Cantheconidae
Our Ref: BNT240706LUCN 3
LU503558 furcellata (walff) industrial production method.
To achieve the above object, the present invention adopts the following technical solution:
A use of Hermetia illucens L. as Cantheconidae furcellata (walff) food source.
The Hermetia illucens L. and Bombyx mori L. are jointly used as the
Cantheconidae furcellata (walff) food source;
Preferably, in the food source for every 100 Cantheconidae furcellata (walff), the dosage ratio of the Hermetia illucens L. to the Bombyx mori L. is 30- 100:6-20;
Preferably, the Hermetia illucens L. is a 3-5 instar Hermetia illucens L. larvae;
Preferably, the Bombyx mori L. is a 3-5 instar Bombyx mori L. larvae.
A Cantheconidae furcellata (walff) food source , characterized in that, comprises Hermetia illucens L. and Bombyx mori L..
The Cantheconidae furcellata (walff) food source for every 100
Cantheconidae furcellata (walff) contains 30-100 Hermetia illucens L.s and 6-20 Bombyx mori L.s;
Preferably, the Hermetia illucens L. is a 3-5 instar Hermetia illucens L. larvae;
Preferably, the Bombyx mori L. is a 3-5 instar Bombyx mori L. larvae.
A Cantheconidae furcellata (walff) artificial feeding method , characterized in that, the Cantheconidae furcellata (walff) food source is used to feed the
Cantheconidae furcellata (walff).
Put the Cantheconidae furcellata (walff) food source in the multiplication device to raise the Cantheconidae furcellata (walff);
Preferably, the multiplication device is provided with a multiplication mesh bag; the multiplying mesh bag is a mesh bag structure with a built-in support component;
Preferably, the shape and size of the support component is compatible with the shape and size of the inner wall of the mesh bag; the mesh bag structure is closed at one end and open at one end;
Preferably, the support component is a support ring; The mesh bag is a cylindrical structure;
Preferably, the inner diameter of the mesh bag of the cylindrical structure is adapted to the diameter of the support ring;
Our Ref: BNT240706LUCN 4
LU503558
Preferably, the number of support rings is 1-3;
Preferably, the mesh bag structure is further with built-in food net sleeve, feeding water cotton, egg collection card; the Cantheconidae furcellata (walff) food source is placed in the food net sleeve;
Preferably, the egg collection card is a rectangular card folded in half; the egg collection card is snapped between the support ring and the inner wall of the mesh bag through the crease folded in half;
Preferably, the mesh size of the mesh bag structure is 100 mesh, and the mesh size of the food mesh sleeve is 20 mesh;
Preferably, the multiplication device is a multi-layer bracket structure; a support plate is provided on each layer of bracket; at least one support ring groove is provided horizontally on each layer of support plate; the size and shape of notch of the support ring groove is compatible with the size and shape of the peripheral edge entity of the support ring of the multiplication mesh bag, so that the support ring of the multiplication mesh bag can be inserted upright into the support ring groove, snapped and fixed.
A Cantheconidae furcellata (walff) industrial production method, characterized in that, comprises:
Step 1: build several functional movable panel rooms;
Step 2: produce Cantheconidae furcellata (walff) food source;
Step 3: collect Cantheconidae furcellata (walff) insect species;
Step 4: breed Cantheconidae furcellata (walff) insect species;
Step 5: Quality sampling inspection of Cantheconidae furcellata (walff);
The functional movable panel room is provided with air conditioner, heater fan, temperature and humidity meter, humidifier, fluorescent lamp, ultravioletultraviolet lamp, power supply system; the air conditioner, heater fan, humidifier, fluorescent lamp, and ultraviolet lamp are respectively connected to the power supply system through circuit;
The Cantheconidae furcellata (walff) food source is the Cantheconidae furcellata (walff) food source of claim 3 or 4;
In step four, the breed Cantheconidae furcellata (walff) insect species comprises: using said Cantheconidae furcellata (walff) artificial feeding method, the Cantheconidae furcellata (walff) insect species collected in step
Our Ref: BNT240706LUCN
LU503558 three are placed in the multiplicationmultiplication mesh bagmesh bag of the multiplication device of the artificial feeding method for feeding and breeding.
The Cantheconidae furcellata (walff) industrial production method further includes: step six, collection and packaging of Cantheconidae furcellata 5 (walff);
Step 7: Cantheconidae furcellata (walff)Cantheconidae furcellata (walff) are stored in storage;
Preferably, in step two, the functional movable panel room is used as a food source production room, and the functional movable panel room of the food source production room is also provided with a fresh-keeping cabinet, two electric heating barrelbarrels, 20 shelves, 100 mesh covers, and 50 net baskets; The fresh-keeping cabinet and electric heating barrel are respectively connected to the power supply system through circuit; mesh covers and net baskets can be placed on shelves; the mesh covernet sleeve is used to hatch Bombyx mori L. eggs or Hermetia illucens L. eggs that are used as Cantheconidae furcellata (walff) food sources; Net baskets are used to house Bombyx mori L. larvae or Hermetia illucens L. larvae that are used as Cantheconidae furcellata (walff) food sources.
Preferably, in step three, the functional movable panel room is used as a insect species identification and breeding room; the functional movable panel room of the insect species identification and breeding room is also equipped with microscopes, anatomical mirrors and multiplication devices.
Preferably, in step three, said collect Cantheconidae furcellata (walff) insect species refers to: collecting insect species similar to Cantheconidae furcellata (walff) in shrubs, weeds, and tobacco fields in tropical or subtropical areas and bringing them back to the species identification room for microscopic identification, and the species identified as Cantheconidae furcellata (walff) are placed in the multiplication mesh bag of the multiplication device for step four.
Preferably, in step four, a number of functional movable panel houses constructed are used as mating spawning chamber, ovulation chamber, larval feeding chamber, respectively; the functional movable panel room used as a mating spawning room or larval feeding chamber is also equipped
Our Ref: BNT240706LUCN 6
LU503558 with a multiplication device; the functional movable panel room used as an ovulation chamber is also equipped with an ovulation device;
Preferably, in step five, the quality sampling inspection of Cantheconidae furcellata (walff) refers to: random sampling on quality of Cantheconidae furcellata (walff) produced in each batch, and the sampling quantity is 1/200- 1/100 of the batch production quantity.
In step two, feed fed to Hermetia illucens L. is prepared with corn flour, wheat bran, honey, GeosaurusGeosaurus powder and water according to the weight ratio of 20:10:1:1:50; Fresh-keeping cabinets are used to store feed for Hermetia illucens L., and electric heating barrelelectric heating barrels are used to heat feed for Hermetia illucens L.;
Preferably, in step four, temperature of the ovulation is 30-32 °C and humidity is 70+10%; the larvae are reared at a temperature of 27-30 °C, a humidity of 70+10%, and a light of L:D=16:8;
Preferably, in step four, Cantheconidae furcellata (walff) adult after eclosion need to be replaced with a new multiplying mesh bag.
In step six, the functional movable panel room is used as a collection and packaging room; the functional movable panel room of the collection and packaging room is also equipped with analytical balance, insect collector, measuring cylinder, multiplication mesh bag, and release box; insect collector is used to collect and screen larvae or adults of the Cantheconidae furcellata (walff); the measuring cylinder is used to measure the approximate number of eggs of Cantheconidae furcellata (walff);
The collection and packaging of Cantheconidae furcellata (walff) includes: screening and packaging of Cantheconidae furcellata (walff) eggs, screening and packaging of Cantheconidae furcellata (walff) larvae, screening and packaging of Cantheconidae furcellata (walff) adult;
The screening of Cantheconidae furcellata (walff) eggs refers to: selecting
Cantheconidae furcellata (walff) eggs with bright color and complete fullness 4-5 days after spawning;
The screening of Cantheconidae furcellata (walff) larvae refers to: selecting 3-5 instar nymphs of Cantheconidae furcellata (walff) with sound body and strong mobility;
Our Ref: BNT240706LUCN 7
LU503558
The screening of adult Cantheconidae furcellata (walff) refers to: selecting adults with sound body, strong mobility and metamorphosis as consistent
Cantheconidae furcellata (walff) adult.
The packaging refers to: the screened Cantheconidae furcellata (walff) eggs or Cantheconidae furcellata (walff) larvae or adults are loaded into a packaging box filled with straw paper to obtain egg products, larval products, and adult products; each box is filled with 200 Cantheconidae furcellata (walff) eggs or 40-50 Cantheconidae furcellata (walff) larvae or adults;
Preferably, in step seven, the functional movable panel room is used as a refrigerated storage room; the functional movable panel room of the refrigerated storage room also has fresh-keeping cabinet; the fresh-keeping cabinet is used to store egg products, larval products, and adult products obtained in step six;
The storage temperature of egg products is 6-10 °C, and the storage temperature of larval products and adult products is 8-15 °C;
The storage conditions of egg products, larval products and adult products also include: humidity 30%-60%, light for 2h, and disinfection with ultraviolet light once a day for 30 minutes.
The beneficial effects of the present invention are as follows:
The present invention firstly proposes to use Hermetia illucens L. as a
Cantheconidae furcellata (walff) food source , the Cantheconidae furcellata (walff) obtained by fed with said food source has better larval growth and shorter cycle than conventional feeding, and at the same time said food source can make activity of adult worms better, higher uniformity, and significantly improve egg production rate. Based on the new use of Hermetia illucens L., the present invention also provides a Cantheconidae furcellata (walff) food source comprising Hermetia illucens L. and Bombyx mori L., and an Cantheconidae furcellata (walff) artificial feeding method based on the food source. By using the food source and feeding method of the present invention, feeding and cultivating the Cantheconidae furcellata (walff) can further optimize the spawning ratio, breeding cycle, uniformity, activity. On this basis, the present invention also firstly creates a Cantheconidae furcellata (walff) industrial production method, which is systematic, process-
Our Ref: BNT240706LUCN 8
LU503558 oriented and large-scale, which can not only significantly reduce difficulty of local insect species collection, improve the efficiency of collecting
Cantheconidae furcellata (walff), breeding adult larvae, and ovization ratio of eggs, but also efficiently produce egg products, larval products and adult products of Cantheconidae furcellata (walff) to meet needs of pest control or natural enemy culture, as well as fill the gap in Cantheconidae furcellata (walff) industrial production in this field.
Figure 1 is a schematic diagram of a multilayer bracket structure of a multiplication device used in step three of the Cantheconidae furcellata (walff) industrial production method provided by an experimental example of the present invention.
Figure 2 is a schematic diagram of structure of the multiplication mesh bag of multiplication device used in step three of the Cantheconidae furcellata (walff) industrial production method in an experimental example of the present invention.
Figure 3 is a schematic diagram of structure of a multiplication device used in step three of the Cantheconidae furcellata (walff) industrial production method provided by an experimental example of the present invention.
Figure 4 is a schematic diagram of the overall and internal structure of the ovulation device used in step four of the Cantheconidae furcellata (walff) industrial production method provided by an experimental example of the present invention.
Figure 5 is a schematic diagram of structure of a release box used in step four of the Cantheconidae furcellata (walff) industrial production method provided by an experimental example of the present invention.
Markings in the figures are listed as follows: 1-multi-layer bracket structure, 11-support plate, 12-fixing component (support ring groove), 13-column, 14- strut, 15-connecting tube, 2-multiplication mesh bag, 21-mesh bag, 211- closed end, 212-open end, 22-support component (support ring), 23-lifting rope, 24-food net sleeve, 241-food net sleeve opening, 25-feeding water cotton, 26-egg collection card;
Our Ref: BNT240706LUCN 9
LU503558 3 - container (ovulation device), 31 - top opening of container, 32 - humidifying part, 33 - container lid, 331 - vent, 3221 - top opening of humidified part, 322 - cylinder, 3222 - water, 3223 - cotton; 4 - release box, 41-box body, 411-box top opening, 412- vent on box side wall, 413-lid, 4131-vent on lid, 414-drain.
The technical content and technical effects of the present invention are further described in detail below in conjunction with specific embodiments, but which do not limit protection scope of the present invention.
Examples Group 1, a new use of Hermetia illucens L.of the present invention
This group of examples provides a use of Hermetia illucens L. as
Cantheconidae furcellata (walff) food source.
In some examples, the Hermetia illucens L. and Bombyx mori L. are jointly used as the Cantheconidae furcellata (walff) food source;
Preferably, in the food source for every 100 Cantheconidae furcellata (walff), the dosage ratio of the Hermetia illucens L. to the Bombyx mori L. is 30- 100:6-20;
Preferably, the Hermetia illucens L. is a 3-5 instar Hermetia illucens L. larvae;
Preferably, the Bombyx mori L. is a 3-5 instar Bombyx mori L. larvae.
Examples Group 2, a Cantheconidae furcellata (walff) food source of the present invention
This group of embodiments provides a Cantheconidae furcellata (walff) food source , characterized in that, comprises Hermetia illucens L. and Bombyx mori L..
In some examples, the Cantheconidae furcellata (walff) food source for every 100 Cantheconidae furcellata (walff) contains 30-100 Hermetia illucens L.s and 6-20 Bombyx mori L.s;
Preferably, the Hermetia illucens L. is a 3-5 instar Hermetia illucens L. larvae;
Preferably, the Bombyx mori L. is a 3-5 instar Bombyx mori L. larvae.
Our Ref: BNT240706LUCN 10
LU503558
Examples Group 3, Cantheconidae furcellata (walff) artificial feeding method of the present invention
This group of embodiments provides a Cantheconidae furcellata (walff) artificial feeding method. All examples of this group possess the following common features: the Cantheconidae furcellata (walff) food source of any one of examples group 2 is used to feed the Cantheconidae furcellata (walff).
In some embodiments, the Cantheconidae furcellata (walff) food source is placed in the multiplication device to feed the Cantheconidae furcellata (walff);
The multiplication device is an insect multiplication device. As shown in
Figures. 1-3, the insect multiplication device has a multi-layer bracket structure 1; each layer of bracket is horizontally provided with a support plate 11; the support plate 11 is provided with a multiplication mesh bag 2; the multiplication mesh bag 2 is a mesh bag 21 structure, and is provided with a support component 22 supporting the mesh bag 21; each layer of support plate 11 is provided with a fixing component 12 for fixing the support component 22.
In some embodiments, the mesh bag 21 is with a built-in support component 22, and shape and size of the support component 22 corresponds to shape and size of the inner wall of the mesh bag 21; the mesh bag 21 structure is closed at one end 211, and opening at one end 212; in the process of insect breeding, the open end 212 can be tightened with a rubber band. The rubber band can be removed when the insect is released.
Preferably, the outer wall of the multiplication mesh bag 2 is provided with a lifting rope 23.
In some specific embodiments, the multi-layer bracket structure 1 is shown in figure. 1, which is assembled by a column 13, a strut 14, and a support plate 11; the column 13 is a component mounted in the vertical direction, and the strut 14 is installed in the horizontal direction between the columns 13; the column 13 and the strut 14 are connected by a connecting tube 15; the connecting tube 15 is a three-way tube or a four-way tube, which can be commercially obtained; a support plate 11 is mounted on the top end of each column 13 and the plane formed by each strut 14; the support plate 11 is a composite plate. Multilayer
Our Ref: BNT240706LUCN 11
LU503558 bracket structure 1 is a common bracket structure in the art, and the existing bracket can be purchased directly, or assembled with the purchased raw materials according to the structure shown in figure 1.
In a specific embodiment, as shown in figure 2 and figure 3, the support component 22 is a support ring 22; the mesh bag 21 is a cylindrical structure.
Preferably, the inner diameter of the mesh bag 21 of the cylindrical structure is compatible with the diameter of the support ring 22; specifically, the inner diameter of mesh bag 21 is 23cm-25cm;
Preferably, the number of support rings 22 is 1-3.
Ina further embodiment, as shown in figure 2, the mesh bag 21 structure is also built-in with a food net sleeve 24, a feeding water cotton 25, an egg collection card 26; the food source of the insect is placed in the food net sleeve 24. The food net sleeve is a small net sleeve placed separately in the multiplication mesh bag, and the food net sleeve is placed through the opening of the multiplication mesh bag.
The food net sleeve 24 is a small net sleeve structure with one end closed and one end open, and the open end is the food net sleeve opening 241; insect food sources (generally smaller bugs or eggs that the insect preys on) are placed from the food net sleeve opening 241 which can be tightened with rubber bands to prevent food sources from escaping; if it needs to be put into the multiplication mesh bag, it is necessary to untie and remove the rubber band first, and put the food net sleeve containing the food source from the opening end 212 of the multiplication mesh bag into the multiplication mesh bag, and then tie the opening end 212 of the multiplication mesh bag tightly, so that in the mesh bag, the food source can crawl out of the food net sleeve for natural enemy insects to eat, or natural enemy insects crawl into the food net sleeve to eat food sources.
In other embodiments, the egg collection card 26 is a rectangular card fold in half;
The egg collection card 26 is snapped between the inner wall of the support ring 22 and the mesh bag 21 by the crease of the fold in half. Keeping a certain amount of moisture on the paper card attracts insects to lay eggs on it.
In a specific embodiment, the mesh size of the mesh bag 21 structure is 100 mesh, and the mesh size of the food net sleeve 24 is 20 mesh;
In some embodiments, the fixing component 12 is selected from: card slot,
Our Ref: BNT240706LUCN 12
LU503558 fastener, clamp or snap ring.
In a preferred embodiment, as shown in figure 1 or figure 3, the card slot is a support ring groove 12 disposed horizontally as a whole on the upward plane of the support plate; size and shape of notch the support ring groove 12 is compatible with size and shape of the peripheral edge entity of the support ring 22 of the multiplication mesh bag 2, so that the support ring of the multiplication mesh bag can be inserted upright into the support ring groove, snapped and fixed.
In a specific embodiment, groove wall of the support ring groove 12 is an upward convex perpendicular to plane of the support plate 11, or, a concave plane toward inner of the support plate 11 and perpendicular to plane of the support plate 11; figure 1 and 3 show a support ring groove 12 with a convex structure on plane of the support plate 11 as the groove wall, those skilled in the art according to the teachings of the present invention, may also provide a support ring groove 12 with a concave structure set down on a support plate 11 having a certain thickness.
In a more specific embodiment, the width of the support ring groove 12 is 0.5-1cm, preferably 0.5cm, and the depth is 1-2cm, preferably 1.5cm;
Preferably, the insect is a Cantheconidae furcellata (walff).
Examples Group 4, a Cantheconidae furcellata (walff) industrial production method of the present invention
This group of embodiments provides a Cantheconidae furcellata (walff) industrial production method. All examples of this group have the following common features, said Cantheconidae furcellata (walff) industrial production method comprises:
Step 1: build several functional movable panel rooms;
Step 2: produce Cantheconidae furcellata (walff) food source;
Step 3: collect Cantheconidae furcellata (walff) insect species;
Step 4: breed Cantheconidae furcellata (walff) insect species;
Step 5: Quality sampling inspection of Cantheconidae furcellata (walff).
The functional movable panel room is provided with air conditioner, heater fan, temperature and humidity meter, humidifier, fluorescent lamp, ultraviolet lamp, power supply system; the air conditioner, heater fan, humidifier,
Our Ref: BNT240706LUCN 13
LU503558 fluorescent lamp, and ultraviolet lamp are respectively connected to the power supply system through circuit;
The Cantheconidae furcellata (walff) food source is the Cantheconidae furcellata (walff) food source of any one of examples group 2;
In step four, the breed Cantheconidae furcellata (walff) insect species comprises: using said Cantheconidae furcellata (walff) artificial feeding method in any one of examples group 3, the Cantheconidae furcellata (walff) insect species collected in step three are placed in the multiplication mesh bag of the multiplication device of the artificial feeding method for feeding and breeding.
In further examples, the Cantheconidae furcellata (walff) industrial production method further includes: step six, collection and packaging of
Cantheconidae furcellata (walff);
Step 7: Cantheconidae furcellata (walff) are stored in storage;
Preferably, in step two, the functional movable panel room is used as a food source production room, and the functional movable panel room of the food source production room is also provided with a fresh-keeping cabinet, two electric heating barrels, 20 shelves, 100 mesh covers, and 50 net baskets;
The fresh-keeping cabinet and electric heating barrel are respectively connected to the power supply system through circuit; mesh covers and net baskets can be placed on shelves; the net sleeve is used to hatch Bombyx mori L. eggs or Hermetia illucens L. eggs that are used as Cantheconidae furcellata (walff) food sources; Net baskets are used to house Bombyx mori
L. larvae or Hermetia illucens L. larvae that are used as Cantheconidae furcellata (walff) food sources. movable panel roomelectric heating barrelmesh cover
Preferably, in step three, the functional movable panel room is used as a insect species identification and breeding room; the functional movable panel room of the insect species identification and breeding room is also equipped with microscopes, anatomical mirrors and multiplication devices.
Preferably, in step three, said collect Cantheconidae furcellata (walff) insect species refers to: collecting insect species similar to Cantheconidae furcellata (walff) in shrubs, weeds, and tobacco fields in tropical or
Our Ref: BNT240706LUCN 14
LU503558 subtropical areas and bringing them back to the species identification room for microscopic identification, and the species identified as Cantheconidae furcellata (walff) are placed in the multiplication mesh bag of the multiplication device for step four.
Preferably, in step four, a number of functional movable panel rooms constructed are used as mating spawning chamber, ovulation chamber, larval feeding chamber, respectively; the functional movable panel room used as a mating spawning room or larval feeding chamber is also equipped with a multiplication device; the functional movable panel room used as an ovulation chamber is also equipped with an ovulation device;
As shown in figure 4, the ovulation device is a container 3 with an top opening 31, and in the container, there is provided with a humidifying part 32; the humidifying part 32 is a cylinder 322 structure with an top opening 3221 and hollow inside; The cylinder contains water 3222, the top opening of the cylinder is stuffed with cotton 3223, cotton 3223 is in contact with the water 3222 in the cylinder 322. The top opening 31 of the container 3 is provided with a container lid 33, and shape and size of the container lid 33 are compatible with shape and size of the top opening 31; The container lid 33 is provided with a plurality of vents 331.
Preferably, in step five, the quality sampling inspection of Cantheconidae furcellata (walff) refers to: random sampling on quality of Cantheconidae furcellata (walff) produced in each batch, and the sampling quantity is 1/200- 1/100 of the batch production quantity.
In other examples, in step two, feed fed to Hermetia illucens L. is prepared with corn flour, wheat bran, honey, Geosaurus powder and water according to the weight ratio of 20:10:1:1:50; Fresh-keeping cabinets are used to store feed for Hermetia illucens L., and electric heating barrels are used to heat feed for Hermetia illucens L.;
Preferably, in step four, temperature of the ovulation is 30-32 °C and humidity is 70+10%; the larvae are reared at a temperature of 27-30 °C, a humidity of 70+10%, and a light of L:D=16:8.
In other examples, in step six, the functional movable panel room is used as a collection and packaging room; the functional movable panel room of the
Our Ref: BNT240706LUCN 15
LU503558 collection and packaging room is also equipped with analytical balance, insect collector, measuring cylinder, multiplication mesh bag, and release box; insect collector is used to collect and screen larvae or adults of the
Cantheconidae furcellata (walff); the measuring cylinder is used to measure the approximate number of eggs of Cantheconidae furcellata (walff),
As shown in figure 5, the release box 4 is a box body 41 having a top opening 411, the side wall of the box body is provided with a plurality of vents 412; the top opening 411 of the box body 41 is also provided with a lid 413 suitable for shape and size of the top opening 411; the lid 413 is provided with a vent 4131; drain 414 is opened at bottom of the box body. The shape of the release box is cup-shaped.
The collection and packaging of Cantheconidae furcellata (walff) includes: screening and packaging of Cantheconidae furcellata (walff) eggs, screening and packaging of Cantheconidae furcellata (walff) larvae, screening and packaging of Cantheconidae furcellata (walff) adult;
The screening of Cantheconidae furcellata (walff) eggs refers to: selecting
Cantheconidae furcellata (walff) eggs with bright color and complete fullness 4-5 days after spawning;
The screening of Cantheconidae furcellata (walff) larvae refers to: selecting 3-5 instar nymphs of Cantheconidae furcellata (walff) with sound body and strong mobility;
The screening of adult Cantheconidae furcellata (walff) refers to: selecting adults with sound body, strong mobility and metamorphosis as consistent
Cantheconidae furcellata (walff) adult.
The packaging refers to: the screened Cantheconidae furcellata (walff) eggs or Cantheconidae furcellata (walff) larvae or adults are loaded into a packaging box filled with straw paper to obtain egg products, larval products, and adult products; each box is filled with 200 Cantheconidae furcellata (walff) eggs or 40-50 Cantheconidae furcellata (walff) larvae or adults;
Preferably, in step seven, the functional movable panel room is used as a refrigerated storage room; the functional movable panel room of the refrigerated storage room also has fresh-keeping cabinet; the fresh-keeping cabinet is used to store egg products, larval products, and adult products
Our Ref: BNT240706LUCN 16
LU503558 obtained in step six;
The storage temperature of egg products is 6-10 °C, and the storage temperature of larval products and adult products is 8-15 °C;
The storage conditions of egg products, larval products and adult products also include: humidity 30%-60%, light for 2h, and disinfection with ultraviolet light once a day for 30 minutes.
One of the most specific embodiments of the present invention provides an
Cantheconidae furcellata (walff) industrial production method, the production process comprises the following steps: 01. Design and construction of facilities and equipment 02. production of Cantheconidae furcellata (walff) food source 03. Collection of native indigenous insect species 04. Amplification of Cantheconidae furcellata (walff) insect species 05. Assembly line breeding of Cantheconidae furcellata (walff) 06. Quality sampling inspection of Cantheconidae furcellata (walff) 07. Collection and packaging of Cantheconidae furcellata (walff) 08. Storage of Cantheconidae furcellata (walff)
The industrial production method of the present invention is simple, which can efficiently and stably carry out the production of Cantheconidae furcellata (walff) on a large scale, , realize the industrial production of mass assembly line, and overcome some problems like of imperfect breeding facilities and equipment for Cantheconidae furcellata (walff), difficulties in collecting native indigenous species, low spawning rate, low ovulation rate; severe mutual predation, long breeding cycle, poor uniformity of the bred Cantheconidae furcellata (walff), insufficient activity, large labor and high energy consumption required in breeding. In all links of production, the technical requirements of light, temperature, humidity, period and other technical requirements have been standardized and controlled, and technical standards for the industrial production of Cantheconidae furcellata (walff) have been established, so that the factory and batch production of
Cantheconidae furcellata (walff) is realized and Cantheconidae furcellata (walff) can be guaranteed for applying in pest control dominated by
Lepidoptera in production of agricultural and forestry crops. At the same time,
Our Ref: BNT240706LUCN 17
LU503558 production cost of Cantheconidae furcellata (walff) is reduced, and the large- scale application and utilization rate of Cantheconidae furcellata (walff) in agriculture and forestry are improved, so that Cantheconidae furcellata (walff) have feasibility in large-scale production and popularization.
Further, in step 01, facility is designed and constructed as:
Main facilities and equipment for Cantheconidae furcellata (walff) industrial production include production room, collection and packaging room, and refrigerated storage room. Among them, the production room includes food source production room, insect species breeding room, ovulation chamber, larval feeding chamber, mating spawning chamber.
The design and construction of facilities and equipment is mainly to design and prepare related facilities and equipment according to scale of
Cantheconidae furcellata (walff) required in production.
Take a production of 2 million head per batch as an example.
The food source production room consists of movable panel room, air conditioner, heater fan, fresh-keeping cabinet, electric heating barrel, shelves, temperature and humidity meter, ultraviolet lamp, fluorescent lamp, power supply and matching switch socket, mesh cover, net basket.
Specific quantity or specifications: 1 movable panel room (length 6 meters, width 4 meters, height 3 meters), 1 air conditioner (3 hp, 7.2 kw), 1 heater fan (electric heating power 5kw), 1 fresh-keeping cabinet (1350L), 2 electric heating barrels (4.2 kw), 20 shelves (length 1.2 meters, width 0.4 meters, height 1.8 meters, 4 floors), 2 temperature and humidity meter (-10°C-50°C,
RHO-95%), 2 ultraviolet lamps (60w), 6 fluorescent lamps (30w), 1 set of power supply and matching switch sockets (power supply 220v or 380v), 100 mesh covers (length 60cm, width 60cm, height 60cm, 60 mesh), 50 net baskets (length 50cm, width 50cm, height 30cm, 20 mesh).
The room area is 24 square meters, the square area of 20 shelves is 9.6 square meters, and the room can accommodate all 20 shelves. The shelves are mainly used for placing the breeding insects and other materials and materials used in production.
The insect species identification and breeding room consists of a movable panel room, air conditioner, humidifier, temperature and humidity meter,
Our Ref: BNT240706LUCN 18
LU503558 ultraviolet lamp, fluorescent lamp, power supply and matching switch socket, multiplication device (shown in figure 1 figure 3), microscope, and anatomical mirror.
Specific quantity or specifications: 1 movable panel room (length 6 meters, width 4 meters, height 3 meters), 1 air conditioner (3 hp, 7.2 kw), 1 heater fan (electric heating power 5kw), 2 humidifiers (100w), 2 temperature and humidity meter s(-10°C-50°C, RHO0-95%), 2 ultraviolet lamps (60w), 6 fluorescent lamps (30w), 1 set of power supply and matching switch sockets (power supply 220v or 380v), 100 set of multiplication devices (60cm, width 60cm, 60cm high, 100 mesh), 1 microscope (10,000 times), anatomical mirror (10,000 times).
The ovulation chamber consists of a movable panel room, air conditioner, artificial climate chamber, humidifier, temperature and humidity meter, fluorescent lamp, power supply and matching switch socket, and ovulation device (figure 4).
Specific quantity or specifications: 1 movable panel room (length 6 meters, width 4 meters, height 3 meters), 1 air conditioner (3 hp, 7.2 kw), 2 artificial climate chambers (5-35 °C, 5000w), 2 humidifiers (100w), 2 temperature and humidity meters (-10°C-50°C, RH0-95%), 6 fluorescent lamps (30w), 1 set of power supply and matching switch sockets (power supply 220v or 380v), 1000 sets of incubation devices (figure 4) (1000ml, 120 mesh).
The larval feeding chamber consists of a movable panel room, air conditioner, humidifier, heater fan, temperature and humidity meter, fluorescent lamp, ultraviolet lamp, power supply and matching switch socket,
Cantheconidae furcellata (walff) multiplication device.
Specific quantity or specifications: 1 movable panel room (length 6 meters, width 4 meters, height 3 meters), 1 air conditioner (3 hp, 7.2 kw), 2 heater fans (electric heating power 5kw), 2 humidifiers (100w), 2 temperature and humidity meters (-10°C-50°C, RHO0-95%), 6 fluorescent lamps (30w), 2 ultraviolet lamps (60w), 1 set of power supply and matching switch sockets (power supply 220v or 380v), 100 sets of Cantheconidae furcellata (walff) multiplication devices.
The mating spawning room consists of a movable panel room, air conditioner,
Our Ref: BNT240706LUCN 19
LU503558 heater fan, humidifier, temperature and humidity meter, fluorescent lamp, ultraviolet lamp, power supply and matching switch socket, and
Cantheconidae furcellata (walff) multiplication device.
Specific quantity or specifications: 1 movable panel room (length 6 meters, width 4 meters, height 3 meters), 1 air conditioner (3 horses, 7.2 kw), 1 heater fan (electric heating power 5kw), 2 humidifiers (100w), 2 temperature and humidity meters (-10 °C-50 °C, RH0-95%), 6 fluorescent lamps (30w), 2 ultraviolet lamps (60w), 1 set of power supply and matching switch sockets (power supply 220v or 380v), 20 sets of Cantheconidae furcellata (walff) multiplication devices.
The collection and packaging room consists of movable panel room, air conditioner, humidifier, temperature and humidity meter, fluorescent lamp, ultraviolet lamp, power supply and matching switch socket, analytical balance, insect collector, plastic measuring cylinder, Cantheconidae furcellata (walff) multiplication mesh bag, release box.
Specific quantity or specifications: 1 movable panel room (length 6 meters, width 4 meters, height 3 meters), 1 air conditioner (3 hp, 7.2 kw), 2 humidifier (100w), 2 temperature and humidity meter (-10°C-50°C, RHO0-95%), 6 fluorescent lamps (30w), 2 ultraviolet lamps (60w), 1 set of power supply and matching switch sockets (power supply 220v or 380v), 2 analytical balance (accuracy 0.0001g), 2 insect collectors (300Aw), 5 plastic measuring cylinders (500ml), 1000 sets of Cantheconidae furcellata (walff) multiplication mesh bags (100 mesh), 100,000 sets (250ml) of release boxes.
The refrigerated storage room consists of movable panel room, air conditioner, humidifier, shelf, temperature and humidity meter, fluorescent lamp, ultraviolet lamp, power supply and matching switch socket, fresh- keeping cabinet. 1 movable panel room (length 6 meters, width 4 meters, height 3 meters), 2 air conditioners (3 hp, 7.2 kW), 2 humidifiers (100w), 30 shelves (length 1.2m, model 0.4m, height 1.8m, 4 floors), 2 temperature and humidity meters (- 10°C-50°C, RHO0-95%), 6 fluorescent lamps (30W), 2 ultraviolet lamps (60W), 1 set of power supply and matching switch sockets (power supply 220v or 380v), 2 fresh-keeping cabinets (1350L).
Our Ref: BNT240706LUCN 20
LU503558 02. Cantheconidae furcellata (walff) food source production
The food source production room consists of movable panel room, air conditioner, heater fan, fresh-keeping cabinet, electric heating barrel, shelf, temperature and humidity meter, ultraviolet lamp, fluorescent lamp, power supply and matching switch socket, mesh cover, mesh basket, tray, corrugated paper, straw paper.
Specific quantity or specifications: 1 movable panel room (length 6 meters, width 4 meters, height 3 meters), 1 air conditioner (3 hp, 7.2 kw), 1 heater fan (electric heating power 5kw), 1 fresh-keeping cabinet (1350L), 2 electric heating barrels (4.2 kw), 20 shelves (length 1.2 meters, model 0.4 meters, height 1.8 meters, 4 floors), 2 temperature and humidity meters (-10°C-50°C,
RHO-95%), 2 ultraviolet lamps (60w), 6 fluorescent lamps (30w), 1 set of power supply and matching switch sockets (power supply 220v or 380v), 100 mesh covers (length 60cm, width 60cm, height 60cm, 60 mesh), 50 mesh baskets (length 50cm, width 50cm, height 30cm, 60 mesh 4, 30 mesh 10, 10 mesh 36), 200 trays (length 25cm, width 12cm, height Gem), 800 sheets of corrugated paper (grass green length 20cm, width 10cm), 1kg straw paper (sheet 40cm, width 20cm). (1) Prepare and place the relevant facilities and equipment of the food source production room
Resettlement period: prepared in 40-50 days before the collection of native indigenous species of step 03.
Placement principles: safe, standardized, neat, convenient (2) Preparation of other required materials
The other required materials include: Hermetia illucens L.species,
Bombyxmori L. species, corn flour, wheat bran, honey, Geosaurus powder, fresh mulberry leaves.
Material quantity: 2000 Hermetia illucens L. species, 200 Bombyxmori L. species, corn flour 1000kg, wheat bran 500kg, honey 50kg, Geosaurus powder 50kg, fresh mulberry leaves 0.5-10kg/day (prepared after silkworm ovulation). (3) Insect source breeding technology
Our Ref: BNT240706LUCN 21
LU503558 2.3.1 Hermetia illucens L. breeding technology
Production of Hermetia illucens L. eggs: release the Hermetiaillucens L. species into the mesh cover, release 1000 head per mesh cover, fill the tray with 5% honey water for 1 cm and put it into the mesh cover with the Hermetia illucens L. species, and put 1-2 sheets of corrugated paper into each mesh cover. The corrugated paper is sprayed with water once a day, the corrugated paper can be wet, honey water is added every day according to the situation of the
Hermetiaillucens L. eating honey water, and new corrugated paper is moved into the mesh cover every 2 days to replace the corrugated paper with worm eggs. At this stage, temperature 25+2°C, humility 70%, natural light are kept indoor.
Hermetia illucens L. feed production method: take an electric heating barrel and put 50 parts of water to boil, put corn flour: wheat bran: honey:
Geosaurus powder: clean water according to the ratio of 20:10:1:1:50 for homogenizing, then pour into boiling water in the electric heating barrel and cook for 10 minutes, then cool to normal temperature for backup. The above- mentioned Hermetiaillucens L. feed is commonly used in breeding Hermetia illucens L. in this field.
Production of Hermetia illucens L. larvae: Place the tray with 1 cm of spare
Hermetia illucens L. feed, and then put the edge of the corrugated paper with
Hermetia illucens L. eggs that begins to hatch on the tray, the egg mass is mainly suspended above the feed, and then placed in the mesh cover, in each of which can be placed with two said trays. After that, according to the feeding situation of the Hermetia illucens L., an appropriate amount of feed is added, and at this stage, indoor temperature is maintained at 26+4 °C, humidity 50-80%, and natural light. When the Hermetia illucens L. breeds to 3-5 instars, it can be used as a Cantheconidae furcellata (walff) food source. 2.3.2 Bombyxmori L. breeding technology
Production of Bombyxmori L. eggs: release the Bombyxmori L. species into the mesh cover, release 100 head per mesh cover, and lay 1-2 sheets of straw paper at the bottom of each mesh cover. Spray the straw paper with 5% honey water once a day, moisten the straw paper, and move the new straw paper into the mesh cover every 2 days to replace the straw paper with insect eggs. At this stage, temperature 25+2°C, humidity 70%, and natural
Our Ref: BNT240706LUCN 22
LU503558 light are kept indoor.
Production of Bombyxmori L. larvae: take a 60-mesh net basket, spread fresh mulberry leaves in the net basket, and then spread the straw paper with
Bombyxmori L. eggs that begins to hatch on the fresh mulberry leaves of the net basket, add an appropriate amount of fresh mulberry leaves according to the feeding situation of the Bombyxmori L. larvae, and transfer the
Bombyxmori L. to a 30-mesh net basket after 2 instars, and transfer to a 10- mesh net basket after 3 instars. At this stage, maintain indoor temperature of 26+4°C, humidity of 50-80%, and natural light. When the Bombyxmori L. breeds to 3-5 instars, it can be used as a Cantheconidae furcellata (walff) food source.
During the production process of Cantheconidae furcellata (walff), an ultraviolet lamp is used to disinfect the room once a day, and the disinfection time is 30 minutes. The feed of the Hermetia illucens L. should be put into the fresh-keeping cabinet at 3°C, if it is not used up. It can be heated by electric heating barrel before next use. 03. Collection of native indigenous insect species
The insect breeding room consists of a movable panel room, air conditioner, humidifier, temperature and humidity meter, ultraviolet lamp, fluorescent lamp, power supply and matching switch socket, multiplication device (shown in figure 1-figure 3), microscope, and anatomical mirror.
After the identification of the insect species, it is necessary to put the insect species into the multiplication device immediately for insect species breeding.
Specific quantity or specifications: 1 movable panel room (length 6 meters, width 4 meters, height 3 meters), 1 air conditioner (3 hp, 7.2 kw), 1 heater fan (electric heating power 5kw), 2 humidifiers (100w), 2 temperature and humidity meters (-10°C-50°C, RH0-95%), 2 ultraviolet lamps (60w), 6 fluorescent lamps (30w), 1 set of power supply and matching switch socket (power supply 220v or 380v), 100 sets of multiplication devices (60cm, width 60cm, 60cm high, 100 mesh), 1 microscope (10,000 times), 1 anatomical mirror (10,000 times). (1) Prepare and place the relevant facilities and equipment of the insect
Our Ref: BNT240706LUCN 23
LU503558 species breeding room
Resettlement period: Prepare in 50-60 days before the spread of the
Cantheconidae furcellata (walff) species of step 04.
Placement principles: safe, standardized, neat, convenient (2) Preparation of other required materials
The other required materials include: insect species collection bags
Number of materials: 10 insect species collection bags.
Species collection techniques
In general, insect species of Cantheconidae furcellata (walff) are collected from shrubs, weeds and tobacco fields in tropical or subtropical areas. It is found that the insect species similar to Cantheconidae furcellata (walff) is found, collected with leaves in collection bags and brought back to the insect species breeding room, and the species identified as Cantheconidae furcellata (walff) with microscope and anatomical microscope are used as native indigenous insect species to breed. 04.Cantheconidae furcellata (walff) species spread
The insect species of Cantheconidae furcellata (walff) are transferred into the multiplication mesh bag of the multiplication device, and the food net sleeve is respectively loaded with 30-100 larvae of 3-5 instars
HermetiaillucensL. + 6-20 larvae of 3-5 instars Bombyxmori L. Then, each food net sleeve opening is tied with a rubber band, and the food net sleeve containing the food source is put into the multiplication mesh bag. Feeding water cotton is then put into each multiplication mesh bagfeeding water cotton. Feeding water cotton is 3cm long and 3cm wide square medium density sponges, sponges fully absorbent but not dripping out is appropriate.
The food net sleeve is changed according to the feeding situation of the
Cantheconidae furcellata (walff).
The above food source can provide 200-500 Cantheconidae furcellata (walff) for consumption. After the food net sleeve is tied, the Cantheconidae furcellata (walff) can eat bugs inside through 20 mesh holes in a nylon mesh. .
At this stage, temperature 28+2 °C, humidity 40-80%, fluorescent light for 7h or natural light are kept indoor. 05. Cantheconidae furcellata (walff) assembly line breeding
Our Ref: BNT240706LUCN 24
LU503558 5.1 Production of Cantheconidae furcellata (walff) eggs
The mating spawning room consists of a movable panel room, air conditioner, heater fan, humidifier, temperature and humidity meter, fluorescent lamp, ultraviolet lamp, power supply and matching switch socket, and
Cantheconidae furcellata (walff) multiplication device.
Specific quantity or specifications: 1 movable panel room (length 6 meters, width 4 meters, height 3 meters), 1 air conditioner (3 hp, 7.2 kw), 2 heater fans (electric heating power 5kw), 2 humidifiers (100w), 2 temperature and humidity meters (-10 °C-50 °C, RH0-95%), 6 fluorescent lamps (30w), 2 ultraviolet lamps (60w), one set of power supply and matching switch sockets (power supply 220v or 380v), 20 sets of Cantheconidae furcellata (walff) multiplication devices. 5.1.1 Adult Cantheconidae furcellata (walff) is paired and reared
The Cantheconidae furcellata (walff) adult, most of which are with initial eclosion and havve become adults, are placed in a multiplication mesh bag of the device (8). The device (8) contains a food net sleeve (9), a feeding water cotton (10), and an egg collection card (15) for the Cantheconidae furcellata (walff) to feed and lay eggs.
In this step, the adult Cantheconidae furcellata (walff) need to be replaced with a new multiplication mesh bag, for the following reasons: on the one hand, the production technology of the invention is required, on the other hand, it meets the need for cleaning, sanitation and disinfection.
The egg collection card can be a piece of paper or paper card, such as printer paper or kraft paper; keeping the egg collection card wet most of the time can induce the Cantheconidae furcellata (walff) to lay eggs on the card, which is more effective.
The specific operation is: put 30-100 Hermetia illucens L. larvae and 6-20
Bombyxmori L. larvae into the food net sleeve (9) as food for the
Cantheconidae furcellata (walff) and tie the net sleeve opening tightly with a rubber band to prevent the insects from escaping, put the mesh cover loaded with food and feeding water cotton into the device (8), and then put 100 male and female adult Cantheconidae furcellata (walff) after eclosion into the multiplication mesh bag, and replace the new food net bag and feeding water
Our Ref: BNT240706LUCN 25
LU503558 cotton every day according to the feeding situation of the Cantheconidae furcellata (walff).
The feeding temperature is 27-30 °C, the humidity is 70+10%, and the light is L:D=16:8. 5.1.2 Collection of Cantheconidae furcellata (walff) eggs
Adult Cantheconidae furcellata (walff) begin mating about a week after eclosion and begin laying eggs about 5 days after mating. When the
Cantheconidae furcellata (walff) begins to lay eggs, simply collect the egg collection card (15) in the multiplication mesh bag (8) every day and replace it with a new egg collection card. The egg collection card (15) is a rectangular card fold in half, which is fixed in a multiplication mesh bag by a support ring (7) for easy removal out. Due to the need for a secluded and quiet environment for laying eggs, a piece of black cloth is placed in the middle of the top of the multiplication mesh bag where the egg collection card is placed to facilitate laying eggs and egg collection. 5.2 Hatching of Cantheconidae furcellata (walff) eggs
The ovulation chamber consists of movable panel room, air conditioner, artificial climate chamber, humidifier, temperature and humidity meter, fluorescent lamp, power supply and matching switch socket, and ovulation device (figure 4).
Specific quantity or specifications: 1 movable panel room (length 6 meters, width 4 meters, height 3 meters), 1 air conditioner (3 hp, 7.2 kw), 2 artificial climate chambers (5-35 °C, 5000w), 2 humidifiers (100w), 2 temperature and humidity meters (-10°C-50°C, RH0-95%), 6 fluorescent lamps (30w), 1 set of power supply and matching switch sockets (power supply 220v or 380v), 1000 sets of incubation devices (1000ml, 120 mesh). 5.2.1 Hatching management of Cantheconidae furcellata (walff)
The egg collection cards collected daily are placed into a new multiplication mesh bag for incubation, in which case only feeding water cotton is provided in the multiplication mesh bag. Incubation temperature is 30-32 ° C and humidity is 70+10%.
The spawning rate and the ovulation rate of Cantheconidae furcellata (walff) by conventional breeding methods are 85-90% and 80-85%, the breeding cycle is
Our Ref: BNT240706LUCN 26
LU503558 about 47-65 days, the uniformity is about 88%, and the activity is average. The average body weight per 100 insects is 30g. The average migration distance per minute is 1.98m.
The spawning rate and ovulation rate of Cantheconidae furcellata (walff) bred by the above steps of the invention are 95-100%, 93-98%, and the breeding cycle is about 37-57 days (currently, the whole breeding cycle is 5-8 days of ovation, 25- 35 days of larval development, 4-8 days of adult-mating, and 3-6 days of oviposition after mating), the uniformity is more than 99%, and the activity is strong. The average body weight per 100 insects is 35.8g. The average migration distance of the insect body per minute is 2.7m. 5.3 Production of Cantheconidae furcellata (walff) larvae
The larval feeding chamber consists of movable panel room, air conditioner, humidifier, heater fan, temperature and humidity meter, fluorescent lamp, ultraviolet lamp, power supply and matching switch socket, Cantheconidae furcellata (walff) multiplication device.
Specific quantity or specifications: 1 movable panel room (length 6 meters, width 4 meters, height 3 meters), 1 air conditioner (3 hp, 7.2 kw), 1 heater fan (electric heating power 5kw), 2 humidifier (100w), 2 temperature and humidity meter (-10°C-50°C, RH0-95%), 6 fluorescent lamps (30w), 2 ultraviolet lamps (60w), 1 set of power supply and matching switch sockets (power supply 220v or 380v), 100 set of Cantheconidae furcellata (walff) multiplication devices. 5.3.1 Rearing of Cantheconidae furcellata (walff) larvae 5.3.2 Management of Cantheconidae furcellata (walff) larvae
Honey water is added to the feeding water cotton after the eggs hatch in the mesh bag. After the eggs grow into one instar, the food net sleeve with food loaded are put into for the larvae to eat. According to the growth status of Cantheconidae furcellata (walff), new food and feeding water cotton are changed and added every day.
The feeding temperature is 27-30 °C, humidity is 70+10%, and light is
L:D=16:8. 06. Quality sampling inspection of Cantheconidae furcellata (walff) 06.1 Selection of quality sampling objects of Cantheconidae furcellata (walff)
Our Ref: BNT240706LUCN 27
LU503558
Random sampling shall be used for sampling quality inspection of each batch of
Cantheconidae furcellata (walff) produced, and the quantity of sampling shall be 1/200-1/100 of the quantity produced in the batch. 06.2 Determination of the quality of the sampling object
Egg products: Determine the egg hatching rate of the sample, egg weight per 1000 eggs and milliliters...
Larval and adult products: the number of daily predatory food-source insects of the sampling subjects, the weight per 100 worm body, and the migration distance of the worm body per minute are measured. 07. Collection and packaging of Cantheconidae furcellata (walff)
The collection and packaging room consists of movable panel room, air conditioner, humidifier, temperature and humidity meter, fluorescent lamp, ultraviolet lamp, power supply and matching switch socket, analytical balance, insect collector, plastic measuring cylinder, Cantheconidae furcellata (walff) multiplication mesh bag, release box.
Specific quantity or specifications: 1 movable panel room (length 6 meters, width 4 meters, height 3 meters), 1 air conditioner (3 hp, 7.2 kw), 2 humidifiers (100w), 2 temperature and humidity meters (-10°C-50°C, RHO- 95%), 6 fluorescent lamps (30w),2 ultraviolet lamps (60w), 1 set of power supply and matching switch sockets (power supply 220v or 380v), 2 analytical balances (accuracy 0.0001g), 2 insect collectors (300Aw), 5 plastic measuring cylinders (for measuring the approximate number of
Cantheconidae furcellata (walff) eggs) (500ml), 1000 sets of multiplication mesh bags (100 mesh) and 100,000 sets of release boxes (100 mesh) (figure 5).
The function of the plastic measuring cylinder is to measure the approximate number of Cantheconidae furcellata (walff) eggs, and the approximate volume of egg mass can be obtained by placing the egg mass of
Cantheconidae furcellata (walff) in the measuring cylinder, and then the approximate number of eggs can be obtained by dividing the approximate volume of egg mass by egg mass volume by single egg volume of
Cantheconidae furcellata (walff). 07.1 Collection and packaging of Cantheconidae furcellata (walff) eggs
Our Ref: BNT240706LUCN 28
LU503558 07.1.1 Selection of egg product objects of Cantheconidae furcellata (walff)
Select the bright, complete and full eggs 4-5 days after spawning of
Cantheconidae furcellata (walff). 07.1.2 Methods for collecting and packaging of Cantheconidae furcellata (walff) eggs
Remove impurities on the surface of the eggs, put a layer of straw paper on the bottom of the pre-prepared packaging container (packaging box), load 200 eggs/box, and then put a layer of straw paper on the eggs. 07.2 Collection and packaging of Cantheconidae furcellata (walff) larval products 07.2.1 Selection of product objects of Cantheconidae furcellata (walff) larval
Choose healthy and mobile Cantheconidae furcellata (walff) 3-5 instar nymphs. 07.2.2 Methods for collecting and packaging Cantheconidae furcellata (walff) larval products
Collect the Cantheconidae furcellata (walff) nymphs of the same age, sound body and strong mobility with an insect collector, and then carefully transfer them to a box pre-lined with a layer of straw paper according to the amount of 50 heads/box, and at the same time add 3-5 Cantheconidae furcellata (walff) food source (Hermetia illucens L. larval), and add a drinking device (a plastic finger tube containing 2/3 water, the opening of the finger tube is plugged with an appropriate amount of skim cotton wool), and fixed with tape to the inner wall of the release box. 07.3 Collection and packaging of adultCantheconidae furcellata (walff) 07.3.1 Selection of adult Cantheconidae furcellata (walff) product objects
Cantheconidae furcellata (walff) adults with sound body, strong mobility, and after metamorphosis are selected for approximately at the same adult stage. 07.3.2 Methods for collecting and packaging adult Cantheconidae furcellata (walff)
Collect the adult Cantheconidae furcellata (walff) that are about the same, sound body and strong mobility at the adult stage of the Cantheconidae furcellata (walff) with an insect collector, and then carefully transfer them to a box pre-lined with a layer of straw paper according to the amount of 40
Our Ref: BNT240706LUCN 29
LU503558 heads per box, and at the same time add 3-5 Cantheconidae furcellata (walff) food source (Hermetia illucens L.), and add a drinking device (a plastic finger tube containing 2/3 water, and the opening of the finger tube is plugged with an appropriate amount of skim cotton pad), and fixed with tape on the inner wall of the release box. 08. Cantheconidae furcellata (walff) are stored in storage
The refrigerated storage room consists of movable panel room, air conditioner, humidifier, shelf, temperature and humidity meter, fluorescent lamp, ultraviolet lamp, power supply and matching switch socket, fresh- keeping cabinet. 1 movable panel room (length 6 meters, width 4 meters, height 3 meters), 2 air conditioners (3 hp, 7.2 kw), 2 humidifiers (100w), 30 shelves (length 1.2m, model 0.4m, height 1.8m, 4 floors), 2 temperature and humidity meters (- 10°C-50°C, RHO-95%), 6 fluorescent lamps (30w), 2 ultraviolet lamps (60w), one set of power supply and matching switch sockets (power supply 220v or 380v), 2 fresh-keeping cabinets (1350L). 8.1 Cantheconidae furcellata (walff) into storage, storage and processing 8.1.1 Add the food source and moisture required during storage of the
Cantheconidae furcellata (walff) in the box 8.1.2 Marking of Signage
Stick a label at a unified position above the packaging box of the
Cantheconidae furcellata (walff), and fill in the storage date, insect instar, quantity, batch, prevention and control object, prevention and control area, and use method. 8.2 Management of inbound storage period
Management of temperature and humidity, light, disinfection, etc. during the storage period.
The temperature of the refrigerated storage room is controlled according to the storage period of the products. The temperature of larvae and adult products is 8-15°C, the temperature of egg products is 6-10°C, the humidity is 30%-60%, the light is lit for 2h, and the ultraviolet lamp is disinfected once a day for 30 minutes.
Claims (10)
1. A use of Hermetia illucens L. as Cantheconidae furcellata (walff) food source.
2. The use of Hermetia illucens L. as Cantheconidae furcellata (walff) food source according to claim 1, characterized in that, Hermetia illucens L. and Bombyx mori L. are jointly used as the Cantheconidae furcellata (walff) food source; and/or, in the food source for every 100 Cantheconidae furcellata (walff), the dosage ratio of the Hermetia illucens L. to the Bombyx mori L. is 30-100:6-20; and/or, the Hermetia illucens L. is a 3-5 instar Hermetia illucens L. larvae; and/or, the Bombyx mori L. is a 3-5 instar Bombyx mori L. larvae.
3. A Cantheconidae furcellata (walff) food source, characterized in that, comprises Hermetia illucens L. and Bombyx mori L.
4. The Cantheconidae furcellata (walff) food source according to claim 3, the Cantheconidae furcellata (walff) food source for every 100 Cantheconidae furcellata (walff) contains 30-100 Hermetia illucens L.s and 6-20 Bombyx mori L.s; and/or, the Hermetia illucens L. is a 3-5 instar Hermetia illucens L. larvae; and/or, the Bombyx mori L. is a 3-5 instar Bombyx mori L. larvae.
5. A Cantheconidae furcellata (walff) artificial feeding method, characterized in that, the Cantheconidae furcellata (walff) food source of claim 3 or 4 is used to feed the Cantheconidae furcellata (walff).
6. A Cantheconidae furcellata (walff) artificial feeding method according to claim 5, characterized in that, put the Cantheconidae furcellata
Our Ref: BNT240706LUCN 2 LU503558 (walff) food source in the multiplication device to raise the Cantheconidae furcellata (walff), and/or, the multiplication device is provided with a multiplication mesh bag; the multiplying mesh bag is a mesh bag structure with a built-in support component; and/or, the shape and size of the support component is compatible with the shape and size of the inner wall of the mesh bag; the mesh bag structure is closed at one end and open at one end; and/or, the support component is a support ring; The mesh bag is a cylindrical structure; and/or, the inner diameter of the mesh bag of the cylindrical structure is adapted to the diameter of the support ring; and/or, the number of support rings is 1-3; and/or, the mesh bag structure is further with built-in food net sleeve, feeding water cotton, egg collection card; the Cantheconidae furcellata (walff) food source is placed in the food net sleeve; and/or, the egg collection card is a rectangular card folded in half; the egg collection card is snapped between the support ring and the inner wall of the mesh bag through the crease folded in half; and/or, the mesh size of the mesh bag structure is 100 mesh, and the mesh size of the food mesh sleeve is 20 mesh; and/or, the multiplication device is a multi-layer bracket structure; a support plate is provided on each layer of bracket; at least one support ring groove is provided horizontally on each layer of support plate; the size and shape of notch of the support ring groove is compatible with the size and shape of the peripheral edge entity of the support ring of the multiplication mesh bag.
7. A Cantheconidae furcellata (walff) industrial production method, characterized in that, comprises: Step 1: build several functional movable panel rooms; Step 2: produce Cantheconidae furcellata (walff) food source; Step 3: collect Cantheconidae furcellata (walff) insect species;
Our Ref: BNT240706LUCN 3 LU503558 Step 4: breed Cantheconidae furcellata (walff) insect species; Step 5: Quality sampling inspection of Cantheconidae furcellata (walff); the functional movable panel room is provided with air conditioner, heater fan, temperature and humidity meter, humidifier, fluorescent lamp, ultraviolet lamp, power supply system; the air conditioner, heater fan, humidifier, fluorescent lamp, and ultraviolet lamp are respectively connected to the power supply system through circuit; the Cantheconidae furcellata (walff) food source is the Cantheconidae furcellata (walff) food source of claim 3 or 4; in step four, the breed Cantheconidae furcellata (walff) insect species comprises: using said Cantheconidae furcellata (walff) artificial feeding method of claim 5 or 6, the Cantheconidae furcellata (walff) insect species collected in step three are placed in the multiplication mesh bag of the multiplicationmultiplication device of the artificial feeding method for feeding and breeding.
8. The Cantheconidae furcellata (walff) industrial production method according to claim 7, characterized in that, further includes: step six, collection and packaging of Cantheconidae furcellata (walff); step 7: Cantheconidae furcellata (walff)Cantheconidae furcellata (walff) are stored in storage; and/or, in step two, the functional movable panel room is used as a food source production room, and the functional movable panel room of the food source production room is also provided with a fresh-keeping cabinet, two electric heating barrels, 20 shelves, 100 mesh covers, and 50 net baskets; The fresh-keeping cabinet and electric heating barrel are respectively connected to the power supply system through circuit; mesh covers and net baskets can be placed on shelves; the net sleeve is used to hatch Bombyx mori L. eggs or Hermetia illucens L. eggs that are used as Cantheconidae furcellata (walff) food sources; Net baskets are used to house Bombyx mori L. larvae or Hermetia illucens L. larvae that are used as Cantheconidae furcellata (walff) food sources;
Our Ref: BNT240706LUCN 4 LU503558 and/or, in step three, the functional movable panel room is used as a insect species identification and breeding room; the functional movable panel room of the insect species identification and breeding room is also equipped with microscopes, anatomical mirrors and multiplication devices; and/or, in step three, said collect Cantheconidae furcellata (walff) insect species refers to: collecting insect species similar to Cantheconidae furcellata (walff) in shrubs, weeds, and tobacco fields in tropical or subtropical areas and bringing them back to the species identification room for microscopic identification, and the species identified as Cantheconidae furcellata (walff) are placed in the multiplication mesh bag of the multiplication device for step four; and/or, in step four, a number of functional movable panel houses constructed are used as mating spawning chamber, ovulation chamber, larval feeding chamber, respectively; the functional movable panel room used as a mating spawning room or larval feeding chamber is also equipped with a multiplication device; the functional movable panel room used as an ovulation chamber is also equipped with an ovulation device; and/or, in step five, the quality sampling inspection of Cantheconidae furcellata (walff) refers to: random sampling on quality of Cantheconidae furcellata (walff) produced in each batch, and the sampling quantity is 1/200-1/100 of the batch production quantity.
9. The Cantheconidae furcellata (walff) industrial production method according to claim 8, characterized in that, in step two, feed fed to Hermetia illucens L. is prepared with corn flour, wheat bran, honey, Geosaurus powder and water according to the weight ratio of 20:10:1:1:50; Fresh-keeping cabinets are used to store feed for Hermetia illucens L., and electric heating barrels are used to heat feed for Hermetia illucens L.; and/or, in step four, temperature of the ovulation is 30-32 °C and humidity is 70+10%; the larvae are reared at a temperature of 27-30 °C, a humidity of 70+10%, and a light of L:D=16:8; and/or, in step four, Cantheconidae furcellata (walff) adult after eclosion need to be replaced with a new multiplying mesh bag.
Our Ref: BNT240706LUCN LU503558
10. The Cantheconidae furcellata (walff) industrial production method according to claim 8, characterized in that, in step six, the functional movable panel room is used as a collection and packaging room; the 5 functional movable panel room of the collection and packaging room is also equipped with analytical balance, insect collector, measuring cylinder, multiplication mesh bag, and release box; insect collector is used to collect and screen larvae or adults of the Cantheconidae furcellata (walff); the measuring cylinder is used to measure the approximate number of eggs of Cantheconidae furcellata (walff); the collection and packaging of Cantheconidae furcellata (walff) includes: screening and packaging of Cantheconidae furcellata (walff) eggs, screening and packaging of Cantheconidae furcellata (walff) larvae, screening and packaging of Cantheconidae furcellata (walff) adult; the screening of Cantheconidae furcellata (walff) eggs refers to: selecting Cantheconidae furcellata (walff) eggs with bright color and complete fullness 4-5 days after spawning; the screening of Cantheconidae furcellata (walff) larvae refers to: selecting 3-5 instar nymphs of Cantheconidae furcellata (walff) with sound body and strong mobility; the screening of adult Cantheconidae furcellata (walff) refers to: selecting adults with sound body, strong mobility and metamorphosis as consistent Cantheconidae furcellata (walff) adult; the packaging refers to: the screened Cantheconidae furcellata (walff) eggs or Cantheconidae furcellata (walff) larvae or adults are loaded into a packaging box filled with straw paper to obtain egg products, larval products, and adult products; each box is filled with 200 Cantheconidae furcellata (walff) eggs or 40-50 Cantheconidae furcellata (walff) larvae or adults; and/or, in step seven, the functional movable panel room is used as a refrigerated storage room; the functional movable panel room of the refrigerated storage room also has fresh-keeping cabinet; the fresh-keeping cabinet is used to store egg products, larval products, and adult products
Our Ref: BNT240706LUCN 6 LU503558 obtained in step six;
the storage temperature of egg products is 6-10 °C, and the storage temperature of larval products and adult products is 8-15 °C;
the storage conditions of egg products, larval products and adult products also include: humidity 30%-60%, light for 2h, and disinfection with ultraviolet light once a day for 30 minutes.
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JP4533988B2 (en) * | 2004-11-26 | 2010-09-01 | 国立大学法人京都大学 | Feeding method and feeding apparatus for breeding natural enemy insects |
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