LU503463B1 - PCR amplification primer pair and application thereof - Google Patents

PCR amplification primer pair and application thereof Download PDF

Info

Publication number
LU503463B1
LU503463B1 LU503463A LU503463A LU503463B1 LU 503463 B1 LU503463 B1 LU 503463B1 LU 503463 A LU503463 A LU 503463A LU 503463 A LU503463 A LU 503463A LU 503463 B1 LU503463 B1 LU 503463B1
Authority
LU
Luxembourg
Prior art keywords
pcr amplification
bstd
detection
primer pair
sample
Prior art date
Application number
LU503463A
Other languages
German (de)
English (en)
Inventor
Jing Wang
Teng Qi
Xin Xiong
Yadan Deng
Xi Shu
Feng Zhang
Qingping Zhang
Original Assignee
Chongqing Tianyou Dairy Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chongqing Tianyou Dairy Co Ltd filed Critical Chongqing Tianyou Dairy Co Ltd
Application granted granted Critical
Publication of LU503463B1 publication Critical patent/LU503463B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
LU503463A 2022-07-29 2023-02-09 PCR amplification primer pair and application thereof LU503463B1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202210906762.2A CN115725751A (zh) 2022-07-29 2022-07-29 一种pcr扩增引物对及其应用

Publications (1)

Publication Number Publication Date
LU503463B1 true LU503463B1 (en) 2023-08-10

Family

ID=85292665

Family Applications (1)

Application Number Title Priority Date Filing Date
LU503463A LU503463B1 (en) 2022-07-29 2023-02-09 PCR amplification primer pair and application thereof

Country Status (2)

Country Link
CN (1) CN115725751A (zh)
LU (1) LU503463B1 (zh)

Also Published As

Publication number Publication date
CN115725751A (zh) 2023-03-03

Similar Documents

Publication Publication Date Title
O’Grady et al. Rapid real-time PCR detection of Listeria monocytogenes in enriched food samples based on the ssrA gene, a novel diagnostic target
CN110951898B (zh) 克罗诺杆菌属内4个种的特异性新分子靶标及其快速检测方法
Lawrence et al. Cross comparison of rapid mycoplasma detection platforms
CN111378774A (zh) 一种用于快速检测单核细胞增生李斯特氏菌的引物组、试剂盒及方法
Trnčíková et al. Rapid and sensitive detection of Staphylococcus aureus in food using selective enrichment and real-time PCR targeting a new gene marker
Oliveira et al. Detection of Campylobacter jejuni in naturally contaminated chicken skin by melting peak analysis of amplicons in real-time PCR
LU503463B1 (en) PCR amplification primer pair and application thereof
CN106282354B (zh) 一种鲁氏不动杆菌的检测引物及荧光定量pcr检测方法
Zhou et al. LAMP, PCR, and real-time PCR detection of Acetobacter aceti in yogurt
Gammon et al. Development of real‐time PCR methods for the rapid detection of low concentrations of Gluconobacter and Gluconacetobacter species in an electrolyte replacement drink
LU503460B1 (en) PCR amplification primer pair and probe, and applications thereof
Mohsina et al. qPCR quantification of Carnobacterium maltaromaticum, Brochothrix thermosphacta, and Serratia liquefaciens growth kinetics in mixed culture
CN111020040B (zh) 乳制品中致病菌多重荧光定量pcr检测引物组、试剂盒及其应用
JP2005110587A (ja) アリサイクロバチラス属菌検出用プライマー
CN101712988B (zh) 益生菌乳制品中双岐杆菌的快速定性、定量测定方法
CN111057776B (zh) 李斯特属和6种常见李斯特菌特异性新分子靶标及其快速检测方法
JP2007244348A (ja) ラクトコッカス・ラクティス・サブスピーシーズ・クレモリスｆｃ株検出用プライマーセット、及び該プライマーセットを用いるラクトコッカス・ラクティス・サブスピーシーズ・クレモリスｆｃ株の分子識別法
LU502570B1 (en) PCR amplification primer pair and application thereof
CN111690757A (zh) 用于快速鉴定产呕吐毒素蜡样芽胞杆菌的引物及检测方法
Oliveira et al. A robotic DNA purification protocol and real-time PCR for the detection of Campylobacter jejuni in foods
CN112111584A (zh) 快速检测水中大肠埃希氏菌的方法
WO2023214588A1 (ja) 芽胞形成細菌の検出方法
CN105986029B (zh) 一种鉴别猪源食源菌的液态芯片方法
Manfreda et al. Comparison of the BAX® System with a multiplex PCR method for simultaneous detection and identification of Campylobacter jejuni and Campylobacter coli in environmental samples
Vu et al. Rapid and practical screening method for the detection of colistin-resistant bacteria in food

Legal Events

Date Code Title Description
FG Patent granted

Effective date: 20230810