LU502935B1 - A Premix Feed and Compound Feed for Improving Anti-stress Ability of Weaned rabbits - Google Patents

A Premix Feed and Compound Feed for Improving Anti-stress Ability of Weaned rabbits Download PDF

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LU502935B1
LU502935B1 LU502935A LU502935A LU502935B1 LU 502935 B1 LU502935 B1 LU 502935B1 LU 502935 A LU502935 A LU 502935A LU 502935 A LU502935 A LU 502935A LU 502935 B1 LU502935 B1 LU 502935B1
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parts
feed
weaned
rabbits
weaned rabbits
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LU502935A
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French (fr)
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Hui Diao
Tianwei Wang
Mengjia Zhou
Xiaolan Wei
Chongbo Huang
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Sichuan Animtech Feed Co Ltd
Sichuan Acad Of Animal Science
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/105—Aliphatic or alicyclic compounds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/24—Compounds of alkaline earth metals, e.g. magnesium
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/26—Compounds containing phosphorus
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/30—Oligoelements
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/60—Feeding-stuffs specially adapted for particular animals for weanlings
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/174—Vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes

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Abstract

The invention discloses a premix feed and compound feed for weaned rabbits, wherein, the premix feed for weaned rabbits comprises 10.83-12.5 parts of ferrous fumarate, 5.8-8.7 parts of zinc sulfate monohydrate, 3-4 parts of copper sulfate pentahydrate, 2.36-3.14 parts of manganese sulfate monohydrate, 6.25-8.75 parts of yeast selenium, 0.63-0.78 parts of calcium iodate, 17.5-20 parts of multivitamin, 5-7.5 parts of enzyme preparation, etc. And the compound feed comprises 16-20 parts of corn, 20-25 parts of bran, 6-9 parts of soybean meal, 2-4 parts of rapeseed meal, 8-12 parts of rice bran meal, 30-40 parts of alfalfa meal, 0.8-1.0 part of calcium carbonate, etc. The feed for weaned rabbits prepared by the invention can effectively improve the ADG and F/G of weaned rabbits; improve the apparent digestibility of dry matter, crude protein, crude fiber and ether extract of weaned rabbits.

Description

DESCRIPTION LU502935
A Premix Feed and Compound Feed for Improving Anti-stress Ability of
Weaned rabbits
TECHNICAL FIELD
The invention discloses a premix feed and compound feed for weaned rabbits, belonging to the technical field of animal nutrition and feed science.
BACKGROUND
China is a big rabbit breeder in the world. According to statistics, in the early stage of reform and opening up, the output of meat rabbits in China was 52,500 tons, only accounting for 7.12 of the world's total output. In 1996, there were 220 million rabbits in
China, and the output of rabbit meat was 306,000 tons, accounting for 26.9 and 25.9 of the world total, respectively, surpassing Italy for the first time and ranking first in the world. In 2019, the number of rabbits sold in China reached 470.251 million, and the total output of rabbit meat reached 457,000 tons, accounting for 50.03 and 32.13 of the total global output, respectively, making it the largest country in the world in rabbit meat production. Rabbit meat has the characteristics of high protein, high lysine, high phospholipid, high digestibility, low fat, low cholesterol, low energy and low uric acid. It has high comprehensive nutritional value and health care effect, and is deeply loved by people. In addition, rabbit skin, rabbit hair and other products are one of the important raw materials in the global textile industry.
With the continuous development of meat rabbit industry, intestinal diseases such as enteritis and diarrhea and respiratory diseases such as rhinitis and pneumonia have caused huge economic losses to meat rabbit industry. In order to pursue the growth rate and production performance of meat rabbits, high-dose inorganic trace elements and antibiotics have been added to meat rabbits feed for a long time. However, antibiotics have brought heavy consequences and severe tests to people because of their drug resistance. At the same time, the pollution of heavy metals such as copper and zinc can synergistically screen and promote the horizontal transfer of antibiotic resistance genes, which will aggravate the spread of antibiotic resistance genes in the environment.
Announcement No.194 issued by China's Ministry of Agriculture stipulates that since
July 1, 2020, antibiotics in feed will be completely banned, which will inevitably lead to a decline in the production level of aquaculture and a substantial increase in the cost 0f)502935 aquaculture.
Therefore, under the background of "no antibiotics at the feed end", it is of great significance to develop premix feed and compound feed to improve the health of weaned rabbits, reduce the use of antibiotics at the breeding end, improve the anti-stress ability and improve the production performance, so as to promote China's feed industry and meat rabbit breeding industry towards real green safety.
SUMMARY
In view of this, the present invention provides a premix feed and compound feed for weaned rabbits, which solves the problems of using antibiotics and other chemicals to prevent diseases and promote growth in the prior art. The application of the premix feed and the compound feed in the invention can improve the production performance of weaned rabbits, increase the digestibility of nutrients, improve immunity, antioxidant capacity and anti-stress capacity, and reduce the consumption of antibiotics at the breeding end.
In order to achieve the above object, the present invention provides the following technical solutions:
The compound feed for weaned rabbits comprises: 1.5-2.5 parts of premix feed for weaned rabbits, 16-20 parts of corn, 20-25 parts of bran, 6-9 parts of soybean meal, 2-4 parts of rapeseed meal, 8-12 parts of rice bran meal, 30-40 parts of alfalfa meal, 0.8-1.0 part of calcium carbonate, 0.4-0.6 part of calcium hydrogen phosphate, 0.4-0.6 part of salt, 0.05-0.15 part of lysine, 0.02-0.06 part of methionine, 0.10-0.20 part of baking soda, 0.08-0.15 part of magnesium sulfate, 0.1-0.12 part of choline chloride, 0.03-0.1 part of antioxidant and 0.03-0.1 part of mildew inhibitor.
Further, the compound feed for weaned rabbits comprises 2 parts of premix feed for weaned rabbits, 18 parts of corn, 22 parts of bran, 7.5 parts of soybean meal, 3 parts of rapeseed meal, 10 parts of rice bran meal, 35 parts of alfalfa meal, 0.9 part of calcium carbonate, 0.5 part of calcium hydrogen phosphate, 0.5 part of salt, 0.1 part of lysine, 0.05 part of methionine, 0.15 part of baking soda, 0.1 part of magnesium sulfate, 0.1 part of choline chloride, 0.05 part of antioxidant and 0.05 part of mildew inhibitor.
Further, the premix feed for weaned rabbits comprises 10.83-12.5 parts of ferrous fumarate, 5.8-8.7 parts of zinc sulfate monohydrate, 3-4 parts of copper sulfate pentahydrate, 2.36-3.14 parts of manganese sulfate monohydrate, 6.25-8.75 parts of yeast selenium, 0.63-0.78 parts of calcium iodate, 17.5-20 parts of multivitamin, 5-71%502935 parts of enzyme preparation, 40-50 parts of tributyrin, 100-150 parts of sodium humate, 25-50 parts of Bacillus subtilis, 0-25 parts of antibacterial peptide, 0-175 parts of yeast culture and 492.13-788.64 parts of rice hull powder.
Further, the premix feed for weaned rabbits comprises 11.67 parts of ferrous fumarate, 7.25 parts of zinc sulfate monohydrate, 3.6 parts of copper sulfate pentahydrate, 2.83 parts of manganese sulfate monohydrate, 7.5 parts of yeast selenium, 0.7 parts of calcium iodate, 19 parts of multivitamin, 6 parts of enzyme preparation, 45 parts of tributyrin, 125 parts of sodium humate, 40 parts of Bacillus subtilis and 731.45 parts of rice hull powder.
Further, the premix feed for weaned rabbits comprises 11.67 parts of ferrous fumarate, 7.25 parts of zinc sulfate monohydrate, 3.6 parts of copper sulfate pentahydrate, 2.83 parts of manganese sulfate monohydrate, 7.5 parts of yeast selenium, 0.7 parts of calcium iodate, 19 parts of multivitamin, 6 parts of enzyme preparation, 45 parts of tributyrin, 125 parts of sodium humate, 40 parts of Bacillus subtilis, 20 parts of antibacterial peptide and 711.45 parts of rice hull powder.
Further, the premix feed for weaned rabbits comprises 11.67 parts of ferrous fumarate, 7.25 parts of zinc sulfate monohydrate, 3.60 parts of copper sulfate pentahydrate, 2.83 parts of manganese sulfate monohydrate, 7.50 parts of yeast selenium, 0.70 parts of calcium iodate, 19 parts of multivitamin, 6 parts of enzyme preparation, 45 parts of tributyrin, 125 parts of sodium humate, 40 parts of Bacillus subtilis, 150 parts of yeast culture and 581.45 parts of rice hull powder.
The raw materials used in the compound feed and premix feed for weaned rabbits in the invention can be purchased from the market.
The ferrous fumarate is added in the invention, and its ferrous iron content is 31 parts, which has the characteristics of good palatability, stable chemical property and high biological activity. Ferrous acid can effectively improve the absorption efficiency of iron in weaned rabbits, and has nutritional and physiological functions such as promoting growth, effectively preventing anemia, improving skin color and hair condition, and regulating immunity.
The yeast selenium is added in the invention, which is used as a nutrient supplement to supplement trace element selenium, and also participates in the composition of glutathione peroxidase, so that the yeast selenium has a strong reducing effect on hydrogen or lipid peroxide in vivo, and can protect the integrity of cell membrane structure. In addition, yeast selenium also regulates the immune function of organism through cellular immunity, humoral immunity and nonspecific immunity. LU502935
The yeast culture is added in the invention, which is formed by deep fermentation of yeast on specially prepared liquid culture solution and grain culture medium, and the end product includes more than 200 kinds of metabolites such as denatured culture medium, yeast cells themselves and extracellular metabolites. Yeast culture can maintain the micro-ecological environment of gastrointestinal tract and improve animal growth performance, improve animal gastrointestinal function and feed utilization rate, reduce breeding cost, balance the body's immune system, reduce the occurrence of diseases, reduce the impact of stress on animals and improve the quality and stability of feed.
Therefore, the use of yeast culture in feed without antibiotics can maintain the normal digestive and absorption function of the gastrointestinal tract of weaned rabbits, resist or reduce the negative effects of various stress factors on growth in the process of breeding and production, improve their health, and help weaned rabbits maximize their production performance.
The probiotic added in the invention is Bacillus subtilis, which can destroy the cell walls of plant cells and promote the releasing of nutrients in cells, produce a large number of various vitamins, amino acids, growth-promoting factors and other easily utilized nutrients. It also can antagonize and competitively repel harmful pathogenic bacteria through the secreted suttilin and the produced spores, and produce short-chain volatile fatty acids such as acetic acid, propionic acid and butyric acid to maintain intestinal tract health.
The coated sodium humate is added in the invention, which will not react with acidify agent in feed and will not change the appearance color of feed. And it can form a film on the surface of the intestinal tract to reduce the damage of harmful substances to the intestinal tract, slowly release in the digestive tract, and adsorb harmful substances such as mycotoxins. In addition, it has a certain convergence effect, and it can keep water and sodium.
The natural endogenous antibacterial peptide is added in the invention, which can inhibit and kill Gram's positive and negative pathogenic bacteria, but has no inhibitory effect on beneficial bacteria lactic acid bacteria and Bacillus, thus increasing the ratio of lactic acid bacteria to Escherichia coli in intestinal contents, improving intestinal screen structure, reducing the contents of serum endotoxin and D- lactic acid, and promoting the healthy growth of weaned rabbits.
The invention has the advantages that: 1, The premix feed for weaned rabbits prepared by the invention can effectively improve the ADG and F/G of weaned rabbits; LU502935 2. The premix feed for weaned rabbits prepared by the invention can increase the apparent digestibility of dry matter, crude protein, crude fiber and ether extract in weaned rabbits; 3. The premix feed for weaned rabbits prepared by the invention can reduce the content of serum MDA, increase the activity of serum SOD, and improve the antioxidant capacity of weaned rabbits; 4. The premix feed for weaned rabbits prepared by the invention can increase the content of IgA, IgG and IgM in serum and improve the immunity of weaned rabbits; 5. The premix feed for weaned rabbits prepared by the invention can reduce the content of serum aldosterone and cortisol, increase the content of serum Hsp70, and improve the anti-stress ability of weaned rabbits.
DETAILED DESCRIPTION OF THE INVENTION
The invention will be described in detail with reference to the following specific embodiments, but the scope of the invention will not be limited.
Embodiment 1
The premix feed and compound feed for weaned rabbits comprises 18 parts of corn, 22 parts of bran, 7.5 parts of soybean meal, 3 parts of rapeseed meal, 10 parts of rice bran meal, 35 parts of alfalfa meal, 0.9 part of calcium carbonate and 0.5 part of calcium hydrogen phosphate, 0.5 part of salt, 0.1 part of lysine, 0.05 part of methionine, 0.15 part of baking soda, 0.1 part of magnesium sulfate, 0.1 part of choline chloride, 0.05 part of antioxidant, 0.05 part of mildew inhibitor and 2 parts of premix feed for weaned rabbits.
Each kilogram of premix feed comprises 11.67 g of ferrous fumarate, 7.25 g of zinc sulfate monohydrate, 3.60 g of copper sulfate pentahydrate, 2.83 g of manganese sulfate monohydrate, 7.50 g of yeast selenium, 0.70 g of calcium iodate, 19 g of multivitamin, 6 g of enzyme preparation, 45 g of tributyrin, 125 g of sodium humate, 40 g of Bacillus subtilis and 731 g of rice hull powder.
Embodiment 2
The premix feed and compound feed for weaned rabbits comprises 16 parts of corn, parts of bran, 6 parts of soybean meal, 2 parts of rapeseed meal, 8 parts of rice bran meal, 30 parts of alfalfa meal, 0.8 part of calcium carbonate and 0.4 part of calcium hydrogen phosphate, 0.4 part of salt, 0.05 part of lysine, 0.02 part of methionine, 0.1 part of baking soda, 0.08 part of magnesium sulfate, 0.1 part of choline chloride, 0.03 part of antioxidant, 0.03 part of mildew inhibitor and 1.5 parts of premix feed for weaned rabbitsus02935
Each kilogram of premix feed comprises 11.67 g of ferrous fumarate, 7.25 g of zinc sulfate monohydrate, 3.60 g of copper sulfate pentahydrate, 2.83 g of manganese sulfate monohydrate, 7.50 g of yeast selenium, 0.70 g of calcium iodate, 19 g of multivitamin, 6 g of enzyme preparation, 45 g of tributyrin, 125 g of sodium humate, 40 g of Bacillus subtilis, 20 g of antibacterial peptide and 731 g of rice hull powder.
Embodiment 3
The premix feed and compound feed for weaned rabbits comprises 20 parts of corn, parts of bran, 9 parts of soybean meal, 4 parts of rapeseed meal, 12 parts of rice bran meal, 40 parts of alfalfa meal, 1 part of calcium carbonate and 0.6 part of calcium hydrogen phosphate, 0.6 part of salt, 0.15 part of lysine, 0.06 part of methionine, 0.2 part of baking soda, 0.15 part of magnesium sulfate, 0.12 part of choline chloride, 0.1 part of antioxidant, 0.1 part of mildew inhibitor and 2.5 parts of premix feed for weaned rabbits.
Each kilogram of premix feed comprises 11.67 g of ferrous fumarate, 7.25 g of zinc sulfate monohydrate, 3.60 g of copper sulfate pentahydrate, 2.83 g of manganese sulfate monohydrate, 7.50 g of yeast selenium, 0.70 g of calcium iodate, 19 g of multivitamin, 6 g of enzyme preparation, 45 g of tributyrin, 125 g of sodium humate, 40 g of Bacillus subtilis, 150 g of yeast culture and 581.45 g of rice hull powder.
Experimental example 1
Feeding experiment
Selecting 396 meat rabbits weaned at 35 days of age, and randomly dividing them into 4 treatments, with 11 repetitions in each treatment and 9 rabbits in each repetition.
Experimental rabbits were kept in stainless steel cages in the same closed rabbit house.
The pre-test period is 7 days, and the test period is 30 days. Feeding the rabbits twice a day at 08:00 and 17: 00, and the feeding amount shall be based on the fact that the rabbits are fully fed and hardly leave any feed. Free drinking water during the experiment.
Cleaning the rabbit house once a day, and spraying and disinfecting the rabbit house once every 4 days. Immunization procedure and other feeding management of weaned rabbits are carried out according to the routine of rabbits farm. The diets of weaned rabbits in each treatment group are as follows: feeding the weaned rabbits in Experiment 1 with the formula of Embodiment 1, feeding the weaned rabbits in Experiment 2 with the formula of Embodiment 2, and feeding the weaned rabbits in Experiment 3 with the formula of Embodiment 3.
The diet formula of the control group is as follows: 18 parts of corn, 22 parts of bran, 7.5 parts of soybean meal, 3 parts of rapeseed meal, 10 parts of rice bran meal, 35 parts of alfalfa meal, 0.9 part of calcium carbonate and 0.5 part of calcium hydrogams02935 phosphate, 0.5 part of salt, 0.1 part of lysine, 0.05 part of methionine, 0.15 part of baking soda, 0.1 part of magnesium sulfate, 0.1 part of choline chloride, 0.05 part of antioxidant, 0.05 part of mildew inhibitor and 2 parts of premix feed for weaned rabbits.
Each kilogram of premix feed comprises 11.67 g of ferrous sulfate monohydrate, 7.25 g of zinc sulfate monohydrate, 3.60 g of copper sulfate pentahydrate, 2.83 g of manganese sulfate monohydrate, 7.50 g of sodium selenite, 0.70 g of calcium iodate, 19 g of multivitamin, 6 g of enzyme preparation, and 945.20 g of rice hull powder.
Collecting feed samples by quartering method, taking about 150 g of the samples in each treatment, putting them into sample bags and marking them, and putting them in a refrigerator at -20°C to store the content of nutrients to be measured. Four days before the end of the test period, collecting feces continuously, mixing them evenly, adding the parts of sulfuric acid and a few drops of toluene into them for nitrogen fixation and corrosion prevention, baking them at 65°C to constant weight, and crushing and sieving through a 40-mesh sieve. Referring to Feed Analysis and Feed Quality Testing
Technology (Zhang Liying, 2003) to determine crude protein (CP), dry matter (DM), ether extract (EE) and crude fiber (CF), and calculating the apparent digestibility of meat rabbit diet nutrients by the endogenous indicator method (AIA method).
At the end of the experiment, selecting a meat rabbit with an average weight in each repetition, taking 3 mL of blood from ear vein of the meat rabbit, standing it in a refrigerator at 4°C for 12 h, centrifuging at 4000 r/min for 10 min, separating the serum and storing at -20°C for later use.
At the beginning and end of the experiment, weighing each rabbit on an empty stomach. During the test period, record the feeding amount in repetition units.
Calculating the ratio of material consumption to weight gain (F/G) according to the average daily weight gain (ADG) and the amount of feed.
Using the kit produced by Nanjing Jiancheng Institute of Bioengineering, combined with ultraviolet spectrophotometer to measure malondialdehyde (MDA), total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), K, Na and lysozyme in serum. And using ELISA kit to determine the contents of serum immunoglobulin A(lgA), immunoglobulin G(lgG), immunoglobulin M(IgM), interferon-y (INF-y), interleukin -10(IL-10), aldosterone, cortisol and heat shock protein 70(Hsp70). The kit is purchased from Beijing Chenglin Biotechnology Co., Ltd., and the operation is carried out according to the instructions.
Sorting out all the data with Excel 2010, and using the One-Way ANOVA test in SAS
8.0 to make statistical analysis. The difference is significant with P < 0.05, and the trend)502935 is 0.05 =P < 0.10. The test results are shown in Table 1 to Table 5.
From Table 1, it can be seen that compared with the control group, in the feeding experiment, the compound feed and premix feed provided by Embodiments 1 and 2 can increase the ADG (P < 0.05) in weaned rabbits by 1-15 days. Compared with the control group, in the feeding experiment, the compound feed and premix feed provided in
Embodiment 2 can reduce the F/G in the weaned rabbits by 1-15 days and increase the feed conversion rate (P < 0.05). Compared with the control group, in the feeding experiment, the compound feed and premix feed provided in Embodiment 3 can increase the ADG in weaned rabbits by 16-30 days, decrease the F/G and increase the feed conversion rate (P < 0.05). In the whole period, compared with the control group, the ADG in weaned rabbits in Embodiment 3 is significantly increased by 1-30 days. And the F/G and the feed conversion rate (P < 0.05) in weaned rabbits in Embodiments 2 and 3 are significantly improved by 1-30 days.
Table 1 Effects of premix feed of the invention with different formulas on growth LU502935 performance of weaned meat rabbits
Control | Embodim | Embodim | Embodim | SE | The value
Items group ent 1 ent 2 ent 3 M of P 0.991 0.982 0.981 0.958 0.0 0.247
Original weight(kg) 12
The weight at the 1.415 1.447 1.448 1.399 0.0 0.051 15th day(kg) 14
The weight at the 1.929 1.961 1.965 1.969 0.0 0.370 30th day(kg) 17 1-15 d 28.288 31.0112 31.1322 | 29.415% | 0.6 0.013
ADG(g) 58 2.3408 2.1632 2.1159 2.289% | 0.0 0.006
FIG
46 16-30 d 34.120 | 34.654 34.654b 38.0782 | 0.6 0.001
ADG(g) 48 3.8002 3.7422 3.7652 3.435P 0.0 0.002
F/G 68 1-30 d 31.273) | 32.705 | 32.788 | 33.643? | 0.4 0.006
ADG(g) 34 3.1042 2.984ab 2.9709 2.9229 0.0 0.004
F/G 33
From Table 2, it can be seen that compared with the control group, in the feeding experiment, the apparent digestibility(P < 0.05) of CF and EE in weaned rabbits can be improved by feeding experiment with the compound feed and premix feed provided in
Embodiment 3. Compared with the control group, in the feeding experiment, the compound feed and premix feed provided in Embodiments 2 and 3 can improve the apparent digestibility(P < 0.05) of DM in weaned rabbits. Compared with the control group, in the feeding experiment, the compound feed and premix feed provided 502935
Embodiments 1, 2 and 3 can improve the apparent digestibility(P < 0.05) of CP in weaned rabbits.
Table 2 Effects of premix feed of the invention with different formulas on nutrient digestibility of weaned meat rabbits om [| ee re
Items SEM group t1 t2 t3 P or [one] vow | mor | vow [7] om
CP 67.214° 71.5582 72.0222 74.3462 0 0.002 or [me] are | | ow [7] om
CF 28.461? 30.467 30.7992 31.9792 8 0.025
HD
EE 59.762 61.629 62.410 64.2692 0.005 ou [mow] wo | cow | ee EE | om 57.299 60.8122 61.8392 63.3062 > 0.002
From Table 3, it can be seen that compared with the control group, in the feeding experiment, the compound feed and premix feed provided by Embodiments 1, 2 and 3 can increase the serum IgG content(P < 0.05) in weaned rabbits. Compared with the control group, in the feeding experiment, the compound feed and premix feed provided in Embodiments 2 and 3 can increase the serum IgM and lysozyme content(P < 0.05) in weaned rabbits. Compared with other groups, the serum IgA and lysozyme contents(P < 0.05) in weaned rabbits in group 3 of Embodiments are significantly increased.
Compared with other groups, the serum INF-y content(P < 0.05) in weaned rabbits in group 2 of Embodiments is significantly increased. The serum lysozyme content(P < 0.05) in weaned rabbits in group 3 is significantly higher than that in groups 1 and 2.
Table 3 Effects of different formulas of premix feed of the invention on serumus02935 immune indexes of weaned meat rabbits
Control | Embodime | Embodime | Embodime The value of
Items SEM group nt 1 nt 2 nt 3 P lgA(ug/mL) | 139.529 | 143.969 147.800P 157.7382 | 2.262 <0.001 1393.14 23.48 lgG(ug/mL) 1551.250P | 1619.980% | 1650.1322 <0.001 0° 7
IgM(ug/mL) | 45.283° 47.931bc 51.7562 55.2172 1.055 <0.001
IL-10(ng/L) | 46.657 45.930 47.778 47.202 0.740 0.358 10.99
INF-y(ng/L) | 870.536" | 880.251P | 1016.0232 | 910.769" <0.001 2
Lysozyme( 28.64 157.780° | 231.562b¢ 320.680 516.3782 <0.001
U/mL) 2
From Table 4, it can be seen that compared with the control group, in the feeding experiment, the compound feed and premix feed provided by Embodiments 1, 2 and 3 can reduce the serum MDA content(P < 0.05) in weaned rabbits. Compared with the control group, in the feeding experiment, the compound feed and premix feed provided in Embodiments 2 and 3 can increase the content(P < 0.05) of serum T-AOC and SOD in weaned rabbits.
Table 4 Effects of premix feed of the invention with different formulas on antioxidant capacity of weaned meat rabbits
The
Control | Embodim | Embodim | Embodim
Items SEM value of group ent 1 ent 2 ent 3
P
MDA(nmol/mL) 6.0972 3.931b 3.960P 3.5979 0.297 <0.001
T-AOC(mmol/L) 1.3220 1.3928b 1.4878 1.492? 0.035 0.007
SOD(U/mL) 84.728° | 91.544° | 96.2798P | 99.858? 1.839 <0.001
CAT(U/mL) 13.255 14.691 14.527 14.740 0.945 0.649
GSH-PX(U/ML) | 120.109 | 122.190 | 127.610 | 128.759 9.405 0.897
From Table 5, it can be seen that compared with the control group, in the feeding experiment, the compound feed and premix feed provided by Embodiments 1, 2 and 3 can increase the Hsp70 content(P < 0.05) of serum in weaned rabbits. Compared with the control group, in the feeding experiment, the compound feed and premix fead)502935 provided in Embodiments 2 and 3 can reduce the content of blood aldosterone and cortisol and the ratio of K/Na in weaned rabbits (P < 0.05). Compared with the group of
Embodiment 1, the serum aldosterone level of weaned rabbits in the groups of
Embodiments 2 and 3 decreased significantly (P < 0.05).
Table 5 Effects of different formulas of premix feed of the invention on the anti-stress ability of weaned meat rabbits
Control | Embodime | Embodime | Embodime
Items SEM value group nt 1 nt 2 nt 3 of P
Fl Cl Cl Cl al [or 314.5652 286.763 278.039 6.757 | <0.001
L) 7?
Hamann | ars [er or Jaan on
Hsp70(ng/L) > 456.8732 448.5102 450.2492 9.411 0.001
According to the above analysis, the premixed feed and compound feed for weaned rabbits provided by the invention can increase the apparent digestibility of dry matter, crude protein, crude fiber and ether extract of weaned rabbits; And they can reduce the content of serum MDA, increase the activity of serum SOD and improve the antioxidant capacity of weaned rabbits; What's more, they can increase the content of IgA, IgG and
IgM in serum to improve the immunity of weaned rabbits; And they can reduce the content of serum aldosterone and cortisol, increase the content of serum Hsp70, improve the anti-stress ability of weaned rabbits, and finally improve the growth performance.
The present invention has been described in detail by general description and specific embodiments. It should be pointed out that those skilled in the field can make several improvements and embellishments without departing from the principle of the present invention, and these improvements and embellishments should also be regarded as the protection scope of the present invention.

Claims (6)

  1. CLAIMS LU502935
    1. A compound feed for weaned rabbits comprises 1.5-2.5 parts of premix feed for weaned rabbits, 16-20 parts of corn, 20-25 parts of bran, 6-9 parts of soybean meal, 2-4 parts of rapeseed meal, 8-12 parts of rice bran meal, 30-40 parts of alfalfa meal, 0.8-1.0 part of calcium carbonate, 0.4-0.6 part of calcium hydrogen phosphate, 0.4-0.6 part of salt, 0.05-0.15 part of lysine, 0.02-0.06 part of methionine, 0.10-0.20 part of baking soda,
    0.08-0.15 part of magnesium sulfate, 0.1-0.12 part of choline chloride, 0.03-0.1 part of antioxidant and 0.03-0.1 part of mildew inhibitor.
    2. The compound feed for weaned rabbits according to claim 1, wherein 2 parts of premix feed for weaned rabbits, 18 parts of corn, 22 parts of bran, 7.5 parts of soybean meal, 3 parts of rapeseed meal, 10 parts of rice bran meal, 35 parts of alfalfa meal, 0.9 part of calcium carbonate, 0.5 part of calcium hydrogen phosphate, 0.5 part of salt, 0.1 part of lysine, 0.05 part of methionine, 0.15 part of baking soda, 0.1 part of magnesium sulfate, 0.1 part of choline chloride, 0.05 part of antioxidant and 0.05 part of mildew inhibitor.
    3. The compound feed for weaned rabbits according to claim 1 or 2, wherein, the premix feed for weaned rabbits comprises 10.83-12.5 parts of ferrous fumarate, 5.8-8.7 parts of zinc sulfate monohydrate, 3-4 parts of copper sulfate pentahydrate, 2.36-3.14 parts of manganese sulfate monohydrate, 6.25-8.75 parts of yeast selenium, 0.63-0.78 parts of calcium iodate, 17.5-20 parts of multivitamin, 5-7.5 parts of enzyme preparation, 40-50 parts of tributyrin, 100-150 parts of sodium humate, 25-50 parts of Bacillus subtilis, 0-25 parts of antibacterial peptide, 0-175 parts of yeast culture and 492.13-788.64 parts of rice hull powder.
    4. The compound feed for weaned rabbits according to claim 3, wherein, the premix feed for weaned rabbits comprises 11.67 parts of ferrous fumarate, 7.25 parts of zinc sulfate monohydrate, 3.6 parts of copper sulfate pentahydrate, 2.83 parts of manganese sulfate monohydrate, 7.5 parts of yeast selenium, 0.7 parts of calcium iodate, 19 parts of multivitamin, 6 parts of enzyme preparation, 45 parts of tributyrin, 125 parts of sodium humate, 40 parts of Bacillus subtilis and 731.45 parts of rice hull powder.
    5. The compound feed for weaned rabbits according to claim 3, wherein, the premix feed for weaned rabbits comprises 11.67 parts of ferrous fumarate, 7.25 parts of zinc sulfate monohydrate, 3.6 parts of copper sulfate pentahydrate, 2.83 parts of manganese sulfate monohydrate, 7.5 parts of yeast selenium, 0.7 parts of calcium iodate, 19 parts of multivitamin, 6 parts of enzyme preparation, 45 parts of tributyrin, 125 parts of sodium humate, 40 parts of Bacillus subtilis, 20 parts of antibacterial peptide and 711.45 parts p0502935 rice hull powder.
    6. The compound feed for weaned rabbits according to claim 3, wherein, the premix feed for weaned rabbits comprises 11.67 parts of ferrous fumarate, 7.25 parts of zinc sulfate monohydrate, 3.60 parts of copper sulfate pentahydrate, 2.83 parts of manganese sulfate monohydrate, 7.50 parts of yeast selenium, 0.70 parts of calcium iodate, 19 parts of multivitamin, 6 parts of enzyme preparation, 45 parts of tributyrin, 125 parts of sodium humate, 40 parts of Bacillus subtilis, 150 parts of yeast culture and
    581.45 parts of rice hull powder.
LU502935A 2022-10-20 2022-10-20 A Premix Feed and Compound Feed for Improving Anti-stress Ability of Weaned rabbits LU502935B1 (en)

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