LU502146B1 - PECTATE LYASE (Pel) MUTANT deltaPelG403, AND ENCODING GENE, PREPARATION METHOD AND USE - Google Patents

PECTATE LYASE (Pel) MUTANT deltaPelG403, AND ENCODING GENE, PREPARATION METHOD AND USE Download PDF

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Publication number
LU502146B1
LU502146B1 LU502146A LU502146A LU502146B1 LU 502146 B1 LU502146 B1 LU 502146B1 LU 502146 A LU502146 A LU 502146A LU 502146 A LU502146 A LU 502146A LU 502146 B1 LU502146 B1 LU 502146B1
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Luxembourg
Prior art keywords
pel
mutant
gene
seq
apelg403
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LU502146A
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German (de)
English (en)
Inventor
Zhenghong Peng
Shengwen Duan
Qi Yang
Yuande Peng
Lifeng Cheng
Ke Zheng
Xiangyuan Feng
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Inst Bast Fiber Crops Caas
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/02—Carbon-oxygen lyases (4.2) acting on polysaccharides (4.2.2)
    • C12Y402/02002—Pectate lyase (4.2.2.2)

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
LU502146A 2021-05-24 2022-05-23 PECTATE LYASE (Pel) MUTANT deltaPelG403, AND ENCODING GENE, PREPARATION METHOD AND USE LU502146B1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202110563930.8A CN113549608B (zh) 2021-05-24 2021-05-24 一种果胶裂解酶突变体ΔPelG403及其编码基因、制备方法和应用

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LU502146B1 true LU502146B1 (en) 2022-11-23

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LU502146A LU502146B1 (en) 2021-05-24 2022-05-23 PECTATE LYASE (Pel) MUTANT deltaPelG403, AND ENCODING GENE, PREPARATION METHOD AND USE

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CN (1) CN113549608B (zh)
LU (1) LU502146B1 (zh)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114645038B (zh) * 2022-03-30 2023-06-09 齐鲁工业大学 一种耐碱果胶裂解酶及应用
CN116515871A (zh) * 2022-04-13 2023-08-01 齐鲁工业大学(山东省科学院) 一种高活性突变果胶裂解酶及应用
CN115772531A (zh) * 2022-11-11 2023-03-10 吉林农业大学 一种果胶酸裂解酶基因突变体及其克隆方法和应用
CN115948378A (zh) * 2023-01-31 2023-04-11 武汉新华扬生物股份有限公司 一种环化碱性果胶裂解酶cyc-Pel3及其应用

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105368811B (zh) * 2014-08-08 2018-08-21 中国科学院微生物研究所 果胶裂解酶及其编码基因和应用
CN105112396B (zh) * 2015-09-24 2019-02-22 南京天凯生物技术股份有限公司 黑曲霉果胶裂解酶的突变体及玉米酒精发酵生产中糖浆的降粘剂
CN105368856B (zh) * 2015-12-09 2018-11-30 吉林大学 一种比活提高的突变型嗜热碱性果胶裂解酶基因、工程菌、酶及其应用
CN111417656A (zh) * 2017-08-23 2020-07-14 西卡肿瘤解决方案有限公司 细胞膜穿透缀合物
CN108588061B (zh) * 2018-04-28 2022-01-28 湖北大学 一种比酶活及热稳定性提高的低温碱性果胶酶突变体
CN111424027B (zh) * 2020-03-31 2022-03-01 江南大学 一种定点突变改造的褐藻胶裂解酶突变体及其应用

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CN113549608B (zh) 2022-03-08

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