LU501602B1 - Biocontrol fermented feed for preventing and treating vibriosis and production method and production equipment thereof - Google Patents
Biocontrol fermented feed for preventing and treating vibriosis and production method and production equipment thereof Download PDFInfo
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- LU501602B1 LU501602B1 LU501602A LU501602A LU501602B1 LU 501602 B1 LU501602 B1 LU 501602B1 LU 501602 A LU501602 A LU 501602A LU 501602 A LU501602 A LU 501602A LU 501602 B1 LU501602 B1 LU 501602B1
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- 230000000443 biocontrol Effects 0.000 title claims abstract description 42
- 206010047400 Vibrio infections Diseases 0.000 title claims abstract description 23
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 23
- 230000004151 fermentation Effects 0.000 claims abstract description 92
- 238000000855 fermentation Methods 0.000 claims abstract description 90
- 241000894006 Bacteria Species 0.000 claims abstract description 37
- 239000000463 material Substances 0.000 claims abstract description 29
- 239000007788 liquid Substances 0.000 claims abstract description 19
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims abstract description 17
- 230000000813 microbial effect Effects 0.000 claims abstract description 12
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 11
- 241000604933 Bdellovibrio Species 0.000 claims abstract description 11
- 240000001046 Lactobacillus acidophilus Species 0.000 claims abstract description 11
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims abstract description 11
- 241000218588 Lactobacillus rhamnosus Species 0.000 claims abstract description 11
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims abstract description 11
- 239000001963 growth medium Substances 0.000 claims abstract description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 12
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 12
- 235000019764 Soybean Meal Nutrition 0.000 claims description 12
- 239000008103 glucose Substances 0.000 claims description 12
- 239000004455 soybean meal Substances 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 241001474374 Blennius Species 0.000 claims description 6
- 108010009736 Protein Hydrolysates Proteins 0.000 claims description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 6
- 240000008042 Zea mays Species 0.000 claims description 6
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 6
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 6
- 229940041514 candida albicans extract Drugs 0.000 claims description 6
- 235000005822 corn Nutrition 0.000 claims description 6
- 230000002255 enzymatic effect Effects 0.000 claims description 6
- 235000013312 flour Nutrition 0.000 claims description 6
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 6
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 6
- 229940099596 manganese sulfate Drugs 0.000 claims description 6
- 235000007079 manganese sulphate Nutrition 0.000 claims description 6
- 239000011702 manganese sulphate Substances 0.000 claims description 6
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 6
- 239000002609 medium Substances 0.000 claims description 6
- YWYZEGXAUVWDED-UHFFFAOYSA-N triammonium citrate Chemical compound [NH4+].[NH4+].[NH4+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O YWYZEGXAUVWDED-UHFFFAOYSA-N 0.000 claims description 6
- 235000011046 triammonium citrate Nutrition 0.000 claims description 6
- 239000001393 triammonium citrate Substances 0.000 claims description 6
- 239000012138 yeast extract Substances 0.000 claims description 6
- 239000000306 component Substances 0.000 claims description 3
- 239000012533 medium component Substances 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 238000005303 weighing Methods 0.000 claims description 3
- 238000010521 absorption reaction Methods 0.000 abstract description 12
- 241001465754 Metazoa Species 0.000 abstract description 5
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 abstract description 4
- 241000607598 Vibrio Species 0.000 abstract description 4
- 230000029087 digestion Effects 0.000 abstract description 4
- 102000004196 processed proteins & peptides Human genes 0.000 abstract description 4
- 108090000765 processed proteins & peptides Proteins 0.000 abstract description 4
- 230000001717 pathogenic effect Effects 0.000 abstract description 3
- 241000607594 Vibrio alginolyticus Species 0.000 abstract description 2
- 241000607618 Vibrio harveyi Species 0.000 abstract description 2
- 241000607272 Vibrio parahaemolyticus Species 0.000 abstract description 2
- 229910021529 ammonia Inorganic materials 0.000 abstract description 2
- 239000003242 anti bacterial agent Substances 0.000 abstract description 2
- 229940088710 antibiotic agent Drugs 0.000 abstract description 2
- 235000014113 dietary fatty acids Nutrition 0.000 abstract description 2
- 239000003814 drug Substances 0.000 abstract description 2
- 229940079593 drug Drugs 0.000 abstract description 2
- 238000005516 engineering process Methods 0.000 abstract description 2
- 229930195729 fatty acid Natural products 0.000 abstract description 2
- 239000000194 fatty acid Substances 0.000 abstract description 2
- 150000004665 fatty acids Chemical class 0.000 abstract description 2
- 210000003608 fece Anatomy 0.000 abstract description 2
- 230000002401 inhibitory effect Effects 0.000 abstract description 2
- 239000010871 livestock manure Substances 0.000 abstract description 2
- 235000016709 nutrition Nutrition 0.000 abstract description 2
- 230000035764 nutrition Effects 0.000 abstract description 2
- 235000019629 palatability Nutrition 0.000 abstract description 2
- 229920000049 Carbon (fiber) Polymers 0.000 description 10
- 239000004917 carbon fiber Substances 0.000 description 10
- 238000001514 detection method Methods 0.000 description 10
- 238000010438 heat treatment Methods 0.000 description 10
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 10
- 239000000523 sample Substances 0.000 description 10
- 238000010564 aerobic fermentation Methods 0.000 description 8
- 239000007789 gas Substances 0.000 description 8
- 238000005273 aeration Methods 0.000 description 6
- 238000009360 aquaculture Methods 0.000 description 5
- 244000144974 aquaculture Species 0.000 description 5
- 238000005276 aerator Methods 0.000 description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 4
- 239000001301 oxygen Substances 0.000 description 4
- 229910052760 oxygen Inorganic materials 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- 241000238557 Decapoda Species 0.000 description 3
- 244000052769 pathogen Species 0.000 description 3
- 241000143060 Americamysis bahia Species 0.000 description 2
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000005201 scrubbing Methods 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 208000004232 Enteritis Diseases 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 210000002816 gill Anatomy 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 210000000087 hemolymph Anatomy 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 210000001630 jejunum Anatomy 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/24—Compounds of alkaline earth metals, e.g. magnesium
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23N—MACHINES OR APPARATUS FOR TREATING HARVESTED FRUIT, VEGETABLES OR FLOWER BULBS IN BULK, NOT OTHERWISE PROVIDED FOR; PEELING VEGETABLES OR FRUIT IN BULK; APPARATUS FOR PREPARING ANIMAL FEEDING- STUFFS
- A23N17/00—Apparatus specially adapted for preparing animal feeding-stuffs
- A23N17/008—Apparatus specially adapted for preparing animal feeding-stuffs for treating of silage, e.g. upgrading with water
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Food Science & Technology (AREA)
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- Biomedical Technology (AREA)
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- Marine Sciences & Fisheries (AREA)
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Abstract
The present invention discloses a biocontrol fermented feed for preventing and treating vibriosis composed of mixed fermentation of biocontrol bacteria liquid and base material. The biocontrol bacteria liquid is obtained by fermenting the bacteria into the culture medium. The microbial strains are composed of bdellovibrio and one or more kind of mixed bacteria with bacillus licheniformis, lactobacillus acidophilus, lactobacillus rhamnosus and brewers yeast. Compared with existing products and technologies, the biocontrol fermented feed prepared by the present invention has a significant inhibitory effect on pathogenic vibrio species such as vibrio parahaemolyticus, vibrio alginolyticus, vibrio harveyi, etc., reduces or even replaces the use of antibiotics and other drugs, and enhances digestion absorption, it can also reduce the discharge of ammonia from animal manure. At the same time, the production equipment adopts a modular fermentation system, the data can be monitored in real time, the fermentation process is completely controllable, the production process is environmentally friendly and the fermentation degree is complete. Deep fermentation can significantly reduce the crude nutrition of grains, increase the content of peptides, short peptides and fatty acids, have good palatability and enhance the rate of digestion and absorption.
Description
BIOCONTROL FERMENTED FEED FOR PREVENTING AND LJ501602
[01] The present invention belongs to the technical field of microbial fermentation feed, and specifically relates to a biocontrol fermented feed for preventing and treating vibriosis and production method and production equipment thereof.
[02] Vibriosis is the most common and harmful disease in shrimp farming. Its main symptoms are generally red legs, yellow gills, broken beards, frontal swords, tail stains, enteritis, jejunum and fluorescent shrimp. The shrimp reacts slowly to the external environment, and the body of some shrimps turn black. The hemolymph extracted from the diseased shrimps coagulates slowly, and the color also changes from normal light blue to reddish. In recent years, due to ecological changes in aquaculture waters, vibrio has become one of the main pathogens in aquaculture. So far, more than 20 pathogens have been reported as the pathogen of vibriosis in marine aquaculture animals.
[03] Since most of the existing fermented feeds contain more bacteria during the fermentation process, they are likely to pollute the water during aquaculture. Vibrio bacteria multiply in the water and cause the outbreak of vibriosis, which leads to a reduction in aquaculture production or even no harvest.
[04] One object of the present invention is to provide a biocontrol fermented feed for preventing vibriosis, and another object of the present invention is to provide a biocontrol fermented feed for preventing and treating vibriosis and production method and production equipment thereof.
[05] According to one aspect of the present invention, a biocontrol fermented feed for preventing and treating vibriosis is provided, the biocontrol fermented feed for preventing and treating vibriosis is composed of mixed fermentation of biocontrol bacteria liquid and base materia. The biocontrol fermented feed for preventing and treating vibriosis is made by the mixed fermentation of biocontrol bacteria liquid and base material. The biocontrol bacteria liquid is obtained by fermenting the bacteria into the culture medium. The microbial strains are composed of bdellovibrio and one or more kind of mixed bacteria with bacillus licheniformis, lactobacillus acidophilus, lactobacillus rhamnosus and brewers yeast. The medium components are: 1-3 parts of glucose, 1-3 parts of yeast extract, 0.5-2 parts of soybean meal, 0.1-0.5 parts of triammonium citrate, 0.2-0.8 parts of magnesium sulfate, 0.1-0.5 parts of manganese sulfate, 1000 water parts; the base material components are: 30-45 parts of bran, 5-10 parts of soybean meal, 10-15 parts of corn flour, 1-5 parts of seaweed enzymatic hydrolysate, 1-5 parts of glucose and 5-10 parts of cell broken yeast.
[06] In some embodiments, the biocontrol bacteria liquid is obtained by double fermentation at 28°C-32°C for 4 days by inserting the microbial strain into the medium, 1 and the effective viable bacteria number is not less than 1x10""pfu/ml. LU501602
[07] In some embodiments, the microbial strains in step 1 include 5-8 parts of bdellovibrio, 0.5-1 parts of bacillus licheniformis, 0.5-2 parts of lactobacillus acidophilus, 0.5-2 parts of lactobacillus rhamnosus, and 0.1-0.5 parts of brewers yeast.
[08] According to another aspect of the present invention, a a production method of biocontrol fermented feed for preventing and treating vibriosis is provided, mainly including the following steps: step 1, adding bdellovibrio and one or more kind of mixed bacteria with bacillus licheniformis, lactobacillus acidophilus, lactobacillus rhamnosus and brewers yeast into the medium formulated by 1-3 parts of glucose, 1-3 parts of yeast extract, 0.5-2 parts of soybean meal, 0.1-0.5 parts of triammonium citrate, 0.2-0.8 parts of magnesium sulfate, 0.1-0.5 parts of manganese sulfate and 1000 parts of water, and the effective viable bacteria number of the biocontrol bacteria liquid is not less than 1x10!""pfu/ml by double fermentation at 28°C-32C for 4 days; step 2, weighing 30-45 parts of bran, 5-10 parts of soybean meal, 10-15 parts of corn flour, 1-5 parts of seaweed enzymatic hydrolysate, 1-5 parts of glucose and 5-10 parts of cell broken yeast, and crushing them with a grinder to 40 mesh powder to get the base material; step 3, putting 5-10 parts of biocontrol bacteria liquid into 50-90 parts of base material, and adding an appropriate amount of water to control the overall moisture of the material to 40%-50% and mix well; step 4, obtaining the fermented feed after being put into fermentation equipment for fermentation process.
[09] Further, the fermentation process in step step 4 includes: step 41, fermentation temperature is 32°C, regular oxygen and exhaust gas, and oxygen four times a day and continued three days for full aerobic fermentation; step 42, after the aerobic fermentation is completed, the air intake is stopped, and the air is periodically exhausted, the fermentation temperature is 28°C, and the anaerobic fermentation is conducted for 6 days to obtain fermented feed.
[10] Further, the microbial strains in step step 1 include 5-8 parts of bdellovibrio,
0.5-1 parts of bacillus licheniformis, 0.5-2 parts of lactobacillus acidophilus, 0.5-2 parts of lactobacillus rhamnosus, and 0.1-0.5 parts of brewers yeast.
[11] According to still another aspect of the present invention, a special fermentation production equipment of biocontrol fermenting feed for preventing and treating vibriosis is provided, and the special fermentation production equipment mainly includes a fermentation tank with a feeding port on a top of the fermentation tank. The 2 feeding port has a removable sealing cover. A bottom of the fermentation tank is LU501602 provided with a microporous aeration pipe. A side wall of the microporous aerator tube has micropores. The microporous aerator tube is connected to an air compressor through an air inlet tube. A filter and an intake valve are respectively provided on the air inlet tube. The fermentation tank is also provided with an exhaust pipe. The exhaust pipe is provided with an exhaust valve. The exhaust pipe is also connected with an exhaust fan.
[12] During the aerobic fermentation process, the intake valve is opened four times a day, the exhaust valve is normally open, the exhaust fan is closed, the air pumped by the air compressor is filtered by the filter and then enters the microporous aeration pipe through the intake pipe, the air is dispersed by the micropores on the side wall of the microporous aeration pipe and then diffuses along the bottom of the fermentation tank into the material contained in the fermentation tank, and discharged from the exhaust pipe.
[13] During the anaerobic fermentation process, the intake valve is closed, the exhaust valve is opened regularly, and the exhaust fan is opened at the same time when the exhaust valve is opened. The gas produced in the fermentation process of the materials contained in the fermentation tank is discharged by the exhaust pipe, and the exhaust fan maintains the negative pressure in the fermentation tank through the exhaust pipe.
[14] Further, the fermentation tank is also provided with a temperature control system. The temperature control system includes a carbon fiber heating tube and a temperature detection probe arranged at the bottom of the fermentation tank, and a temperature controller connected to the carbon fiber heating tube and the temperature detection probe respectively. The temperature controller detects the temperature of the material in the fermentation tank through the temperature detection probe and compares the temperature with a preset value and controls the working state of the carbon fiber heating tube.
[15] Through the temperature control system, during the aerobic fermentation process, the fermentation temperature of the materials in the fermentation tank is controlled to 32 °C.
[16] During the anaerobic fermentation process, control the fermentation temperature of the materials in the fermentation tank to 28°C.
[17] Further, the carbon fiber heating tube is located at the bottom of the fermentation tank and is arranged in a continuous U shape to maintain a constant temperature for the fermentation process. The temperature detection probe is located in the bottom four areas of the fermentation tank and is responsible for transmitting the temperature of the material to the temperature controller in real time. The temperature controller is located in a central control room, the temperature controller is connected to the carbon fiber heating tube and the temperature detection probe to control the fermentation temperature.
[18] Further, An outlet end of the exhaust pipe is also connected with a closed absorption tank having an air outlet. The exhaust fan is arranged in a middle section of the exhaust pipe. The gas in the fermentation tank is drawn by the exhaust fan and then enters the closed absorption tank through the exhaust pipe for scrubbing and absorption, 3 and discharged through the air outlet. The closed absorption tank is filled with activated LU501602 carbon and lime water to remove the gas produced by fermentation.
[19] Further, the fermentation tank is transformed from a 5.89mx2.32mx2.31m container, welded and sealed, and the top of the container has a feeding port with a diameter of 1.0m, and the feeding port can be sealed.
[20] Compared with existing products and technologies, the biocontrol fermented feed prepared by the present invention has a significant inhibitory effect on pathogenic vibrio species such as vibrio parahaemolyticus, vibrio alginolyticus, vibrio harveyi, etc, and can adjust the balance of intestinal and water flora after feeding animals; At the same time, the production equipment adopts a modular fermentation system, the data can be monitored in real time, the fermentation process is completely controllable, the production process is environmentally friendly and the fermentation degree is complete.
Deep fermentation can significantly reduce the crude nutrition of grains, increase the content of peptides, short peptides and fatty acids, have good palatability and enhance the rate of digestion and absorption. The production equipment provided by the present invention is environmentally friendly, efficient and controllable. The biocontrol fermented feed produced by this equipment is particularly suitable for aquatic products, livestock and poultry animals, prevents diseases and enhances immunity, reduces or even replaces the use of antibiotics and other drugs, and enhances digestion absorption, it can also reduce the discharge of ammonia from animal manure.
[21] FIG.1 is a schematic structural diagram of a special fermentation production equipment of biocontrol fermenting feed for preventing and treating vibriosis according to an embodiment of the present invention.
[22] FIG.1 schematically shows a special fermentation production equipment of biocontrol fermenting feed for preventing and treating vibriosis according to an embodiment of the present invention. As shown in the figure, the equipment mainly includes a fermentation tank 1 with a feeding port 11 on a top of the fermentation tank
1.
[23] The feeding port 11 has a removable sealing cover 12.
[24] A bottom of the fermentation tank 1 is provided with a microporous aeration pipe 2.
[25] À side wall of the microporous aerator tube 2 has micropores.
[26] The microporous aerator tube 2 is connected to an air compressor 33 through an air inlet tube 3.
[27] A filter 32 and an intake valve 31 are respectively provided on the air inlet tube
3.
[28] The fermentation tank 1 is also provided with an exhaust pipe 4.
[29] The exhaust pipe 4 is provided with an exhaust valve 41.
[30] The exhaust pipe 4 is also connected with an exhaust fan 5.
[31] An outlet end of the exhaust pipe 4 is also connected with a closed absorption 4 tank 6 having an air outlet 61. LU501602
[32] The exhaust fan 5 is arranged in a middle section of the exhaust pipe 4.
[33] The gas in the fermentation tank 1 is drawn by the exhaust fan 5 and then enters the closed absorption tank 6 through the exhaust pipe 4 for scrubbing and absorption, and discharged through the air outlet 61.
[34] The closed absorption tank 6 is filled with activated carbon and lime water to remove the gas produced by fermentation.
[35] In this embodiment, the fermentation tank 1 is transformed from a
5.89mx2.32mx=2.31m container, welded and sealed, and the top of the container has a feeding port 11 with a diameter of 1.0m, and the feeding port 11 can be sealed.
[36] During the aerobic fermentation process, the intake valve 31 is opened four times a day, the exhaust valve 41 is normally open, the exhaust fan 5 is closed, the air pumped by the air compressor 33 is filtered by the filter 32 and then enters the microporous aeration pipe 2 through the intake pipe 3, the air is dispersed by the micropores on the side wall of the microporous aeration pipe 2 and then diffuses along the bottom of the fermentation tank 1 into the material contained in the fermentation tank 1, and discharged from the exhaust pipe 4.
[37] During the anaerobic fermentation process, the intake valve 31 is closed, the exhaust valve 41 is opened regularly, and the exhaust fan 5 is opened at the same time when the exhaust valve 41 is opened. The gas produced in the fermentation process of the materials contained in the fermentation tank 1 is discharged by the exhaust pipe 4, and the exhaust fan 5 maintains the negative pressure in the fermentation tank 1 through the exhaust pipe 4.
[38] In this embodiment, the fermentation tank 1 is also provided with a temperature control system.
[39] The temperature control system includes a carbon fiber heating tube 7 and a temperature detection probe 8 arranged at the bottom of the fermentation tank 1, and a temperature controller 9 connected to the carbon fiber heating tube 7 and the temperature detection probe 8 respectively.
[40] The temperature controller 9 detects the temperature of the material in the fermentation tank 1 through the temperature detection probe 8 and compares the temperature with a preset value and controls the working state of the carbon fiber heating tube 7.
[41] Through the temperature control system, during the aerobic fermentation process, the fermentation temperature of the materials in the fermentation tank 1 is controlled to 32 °C. During the anaerobic fermentation process, control the fermentation temperature of the materials in the fermentation tank 1 to 28°C.
[42] Specifically, the carbon fiber heating tube 7 is located at the bottom of the fermentation tank 1 and is arranged in a continuous U shape to maintain a constant temperature for the fermentation process. The temperature detection probe 8 is located in the bottom four areas of the fermentation tank 1 and is responsible for transmitting the temperature of the material to the temperature controller 9 in real time. The temperature controller 9 is located in a central control room, the temperature controller is connected to the carbon fiber heating tube 7 and the temperature detection probe 8 to control the fermentation temperature. LU501602
[43] The production equipment mentioned above is mainly used for the production of biocontrol fermented feed for the prevention and treatment vibriosis.
[44] The biocontrol fermented feed for preventing and treating vibriosis is made by the mixed fermentation of biocontrol bacteria liquid and base material. The biocontrol bacteria liquid is obtained by fermenting the bacteria into the culture medium. The microbial strains are composed of bdellovibrio and one or more kind of mixed bacteria with bacillus licheniformis, lactobacillus acidophilus, lactobacillus rhamnosus and brewers yeast. The medium components are: 1-3 parts of glucose, 1-3 parts of yeast extract, 0.5-2 parts of soybean meal, 0.1-0.5 parts of triammonium citrate, 0.2-0.8 parts of magnesium sulfate, 0.1-0.5 parts of manganese sulfate, 1000 water parts; the base material components are: 30-45 parts of bran, 5-10 parts of soybean meal, 10-15 parts of corn flour, 1-5 parts of seaweed enzymatic hydrolysate, 1-5 parts of glucose and 5-10 parts of cell broken yeast.
[45] The biocontrol bacteria liquid is obtained by double fermentation at 28°C-32°C for 4 days by inserting the microbial strain into the medium, and the effective viable bacteria number is not less than 1x10""pfu/ml.
[46] Preferably, the microbial strains include 5-8 parts of bdellovibrio, 0.5-1 parts of bacillus licheniformis, 0.5-2 parts of lactobacillus acidophilus, 0.5-2 parts of lactobacillus rhamnosus, and 0.1-0.5 parts of brewers yeast.
[47] The production method mentioned above of biocontrol fermented feed for preventing and treating vibriosis mainly includes the following steps: step 1, adding bdellovibrio and one or more kind of mixed bacteria with bacillus licheniformis, lactobacillus acidophilus, lactobacillus rhamnosus and brewers yeast into the medium formulated by 1-3 parts of glucose, 1-3 parts of yeast extract, 0.5-2 parts of soybean meal, 0.1-0.5 parts of triammonium citrate, 0.2-0.8 parts of magnesium sulfate, 0.1-0.5 parts of manganese sulfate and 1000 parts of water, and the effective viable bacteria number of the biocontrol bacteria liquid is not less than 1x10!""pfu/ml by double fermentation at 28°C-32°C for 4 days; step 2, weighing 30-45 parts of bran, 5-10 parts of soybean meal, 10-15 parts of corn flour, 1-5 parts of seaweed enzymatic hydrolysate, 1-5 parts of glucose and 5-10 parts of cell broken yeast, and crushing them with a grinder to 40 mesh powder to get the base material; step 3, putting 5-10 parts of biocontrol bacteria liquid into 50-90 parts of base material, and adding an appropriate amount of water to control the overall moisture of the material to 40%-50% and mix well; step 4, obtaining the fermented feed after being put into fermentation equipment for fermentation process.
[48] Wherein, the fermentation process in step step 4 includes: step 41, fermentation temperature is 32°C, regular oxygen and exhaust gas, and oxygen four times a day and continued three days for full aerobic fermentation; step 42, after the aerobic fermentation is completed, the air intake is stopped, and the air is periodically exhausted, the fermentation temperature is 28 °C , and the 6 anaerobic fermentation is conducted for 6 days to obtain fermented feed. LU501602
[49] Further, the microbial strains in step step 1 include 5-8 parts of bdellovibrio,
0.5-1 parts of bacillus licheniformis, 0.5-2 parts of lactobacillus acidophilus, 0.5-2 parts of lactobacillus rhamnosus, and 0.1-0.5 parts of brewers yeast. 7
Claims (3)
1. A biocontrol fermented feed for preventing and treating vibriosis composed of mixed fermentation of biocontrol bacteria liquid and base material, wherein the biocontrol fermented feed for preventing and treating vibriosis is made by the mixed fermentation of biocontrol bacteria liquid and base material, the biocontrol bacteria liquid is obtained by fermenting the bacteria into the culture medium, the microbial strains are composed of bdellovibrio and one or more kind of mixed bacteria with bacillus licheniformis, lactobacillus acidophilus, lactobacillus rhamnosus and brewers yeast, the medium components are: 1-3 parts of glucose, 1-3 parts of yeast extract, 0.5-2 parts of soybean meal, 0.1-0.5 parts of triammonium citrate, 0.2-0.8 parts of magnesium sulfate, 0.1-0.5 parts of manganese sulfate and 1000 water parts; the base material components are: 30-45 parts of bran, 5-10 parts of soybean meal, 10-15 parts of corn flour, 1-5 parts of seaweed enzymatic hydrolysate, 1-5 parts of glucose and 5-10 parts of cell broken yeast.
2. The biocontrol fermented feed for preventing and treating vibriosis according to claim 1, wherein the biocontrol bacteria liquid is obtained by double fermentation at 28°C-32°C for 4 days by inserting the microbial strain into the medium, and the effective viable bacteria number is not less than 1x10""pfu/ml.
3. A production method of biocontrol fermented feed for preventing and treating vibriosis, comprising the following steps: step 1, adding bdellovibrio and one or more kind of mixed bacteria with bacillus licheniformis, lactobacillus acidophilus, lactobacillus rhamnosus and brewers yeast into the medium formulated by 1-3 parts of glucose, 1-3 parts of yeast extract, 0.5-2 parts of soybean meal, 0.1-0.5 parts of triammonium citrate, 0.2-0.8 parts of magnesium sulfate, 0.1-0.5 parts of manganese sulfate and 1000 parts of water, and the effective viable bacteria number of the biocontrol bacteria liquid is not less than 1x10!""pfu/ml by double fermentation at 28°C-32C for 4 days; step 2, weighing 30-45 parts of bran, 5-10 parts of soybean meal, 10-15 parts of 1 corn flour, 1-5 parts of seaweed enzymatic hydrolysate, 1-5 parts of glucose and 5-10 HUS0T602 parts of cell broken yeast, and crushing them with a grinder to 40 mesh powder to get the base material; step 3, putting 5-10 parts of biocontrol bacteria liquid into 50-90 parts of base material, and adding an appropriate amount of water to control the overall moisture of the material to 40%-50% and mix well; step 4, obtaining the fermented feed after being put into fermentation equipment for fermentation process.
2
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| LU501602A LU501602B1 (en) | 2022-03-07 | 2022-03-07 | Biocontrol fermented feed for preventing and treating vibriosis and production method and production equipment thereof |
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| LU501602A LU501602B1 (en) | 2022-03-07 | 2022-03-07 | Biocontrol fermented feed for preventing and treating vibriosis and production method and production equipment thereof |
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