LU500878B1 - Efficient fermentation yeast and organic fertilizer and preparation method thereof - Google Patents

Efficient fermentation yeast and organic fertilizer and preparation method thereof Download PDF

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Publication number
LU500878B1
LU500878B1 LU500878A LU500878A LU500878B1 LU 500878 B1 LU500878 B1 LU 500878B1 LU 500878 A LU500878 A LU 500878A LU 500878 A LU500878 A LU 500878A LU 500878 B1 LU500878 B1 LU 500878B1
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bacillus
organic fertilizer
fermentation
yeast
strains
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LU500878A
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French (fr)
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Hongwei Tan
Gansen Wang
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Hongwei Tan
Yitian Biotechnology Hong Kong Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
    • C05F5/002—Solid waste from mechanical processing of material, e.g. seed coats, olive pits, almond shells, fruit residue, rice hulls
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/141—Feedstock
    • Y02P20/145—Feedstock the feedstock being materials of biological origin
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Mechanical Engineering (AREA)
  • Botany (AREA)
  • Environmental & Geological Engineering (AREA)
  • Fertilizers (AREA)

Abstract

The invention provides efficient fermentation yeast and organic fertilizer, which are prepared by the following methods: preparing dominant fermentation strains including Bacillus subtilis, Bacillus megaterium, Bacillus mucilaeinosus, yeast and Bacillus aspergillus oryzae. Making the dominant fermentation strains into mixed yeast; Uniformly mixing crop straws and rice bran with the mixed yeast, and fermenting to obtain the organic fertilizer produced by the method. The organic fertilizer produced by this method can improve the yield and quality of vegetables.

Description

Description 0500878 Efficient fermentation yeast and organic fertilizer and preparation method thereof Technical Neid This invention relaiss efficient fermentation yeast and organic fertilizer and preparation method thereof, Background in recent years, the production and application of organic fertilizers have attracted much attention, which is regarded as an important tool to improve soit fertility and agricultural product quality, However, the use of chemical fertilizers in cultivated land affects the yield and quality of crops after exceeding a certain limit. Therefore, ff chemical fertilizers are used extensively in the long term, it will pollute the land and crops, and then poison the human body.
Summary in order to soive the problems described above, this invention provides efficient fermentation veast and organic fertilizer and preparation method ihersof.
The technical scheme used by this invention is: efficient fermentation yeast and organic fertilizer are prepared by the following methods: 81, screening dominant fermentation strains including Bacillus subtilis, Bacillus megaterium, Bacillus mucilasinosus, Bacillus saccharomyces, and Bacillus aspergillus oryzas, S2, making mixed yeast by purifying, inoculating, expanding and mixing the dominant fermentation strains, S3, making organic fertilizer, uniformiy mixing the crop straw, rice bran and the mixed veast, and fermenting to obtain organic fertiizer, in which, the weight ratio of crop straw to rice bran is (16-17) (3-4), and 2-5 kg of mixed yeast is added per ton of crop straw.
Through a large number of experiments, the inventors found that by using the fermentation advantages of Bacillus subtilis, Bacillus megaterium, Bacillus muciiasinosus, Bacillus saccharomyces, Bacillus aspergiiius oryzae, and the amylose 0500878 and amylopectin in raw materials were degraded into dextrin and various low-moiscular sugars, such as maltose and glucose. Under the action of protease, the indigestible macromolecular protein was degraded into peplons, polypeptide and various amino acids, and the hard-c-absorb substances such as crude fiber and phytic acid in auxihary materials could be degraded. By adopting the technical scheme, compared with the organic fertilizer fermented by common yeast, the organic fertilizer of the technical scheme of the invention has betier fertilizer efficiency, and makes full use of waste Crop straws, and promotes the sustainable development of the agricultural industry.
As further improvement of the present invention, in ST, the dominant fermentation strain consists of the following components in percentage by mass 20-30% of Bacillus subtilis, 20-25% of Bacillus megaterium, 20-25% of Bacillus muciigeinosus, 15-25% of Bacillus saccharomyces and 15-20% of Bacillus aspergiilus oryzae.
As further improvement of the present invention, the dominant fermentation strain consists of the following components in percentage by mass: 20% of Bacillus subiilis, 20% of Bacillus megaterium, 20% of Bacillus mucilasinosus, 20% of Bacillus saccharomyces and 20% of Bacillus aspergilius oryzae.
As further improvement of the present invention, in $2, the preparation of yeast comprises the following steps: using the dominant strains described in 81 for mixed yeast production, operating under sterile conditions, purifying the dominant strains, inoculating and culiunng, and obtaining the strains as first-class seeds, shake-table amplification, inoculating the obtained first-class seeds into culture container, culiuring the number of amplified bacteria for 3-5 days, and culturing the cultured strains as second-class seeds, mixing the cultivated five kinds of secondary seeds with the substrate to obtain inoculated strains, and culturing for 5-7 days to obtain the mixed yeast, As further improvement of the present invention, in 83, the fermentation is natural fermentation, which includes the following steps: uniformly mixing crop straws, rice bran and the mixed yeast, and stacking into strips with a bottom width of 5-7 meters, a 0500878 top width of 2.5-3.0 meters and a height of 2.5.3.0 meters, and naturally fermenting.
As further improvement of the present invention, in the natural fermentation, the compost is turned every 10-12 days, and after natural fermentation for 25-35 days, the organic fertilizer produced by the method is obtained, As further improvement of the present invention, during fermentation, air is introduced into the fermentation pile through the air pump and the air pipe with air holes.
As further improvement of the present invention, the usage amount of the organic fertilizer is 120-160 kg/mu, applying once a year.
Comparing with prior art, the invention has the following beneficial effects.
First, this scheme uses Bacillus subtilis, Baciius megaterium, Bacillus muciiseinosus, Bacillus saccharomyces, Bacillus aspergilus oryzae io degrade amylose and amylopectin in raw materials into dextrin and various low-moleculer saccharides such as maliose and glucose, and under the action of protease, the indigestible macromolecular protein is degraded into peptone, polypeptide and various amino acids, and the hard-tc-absorb substances such as crude fiber and phytic acid in awdliary materials can be degraded, which meets the requirements of agricultural safety production.
Moreover, according to the technical scheme of the invention, the crop straw is fermented into organic fertilizer, and the crop straw is utilized as a resource, which not only solves the problem of environmental pollution caused by the crop straw and realizes clean production and protects the environment, It can tum waste into treasure, enrich the soi fertility, and increase the vield and efficiency of crops and promote the sustainable development of agricultural industry. Morsover, that production cost of the organic fertilize is very low, which is convenient for popularization and popularization.
Description of the present invention The preferred embodiments of the present invention will be described in further detail,
Embodiment 1 0500878 Efficient fermentation of veast and organic fertilizer is prepared by the following steps: Step À Screering the dominant fermentation strains, and selecting Bacillus subtilis (ACCC11060), Bacillus megaterium (ACCC1GO10), Bacillus mucilasinosus (aiss known as silicale bacteria), ACCC11103), Bacillus saccharomyces (ACCC20055) and Bacillus aspergilius oryzae (ACCC30183) were used as fermentation strains. These five strains are purchased from the National Culture Collection Center, and thelr mass percentages are respectively: Bacillus subtilis 25%, Bacillus megaterum 25%, Bacilius mucilasinosus 20%, veast 15% and Bacillus aspergilus oryzas 15%, that is, the total mass percentage of the five strains is 100%.
Step B Making mixed yeast, using the screened dominant fermentation strains for mixed yeast production, operating under sterile conditions, purifying the dominant strains, inoculating and culturing, and obtaining the strains as first-class sesds, Shaking table amplification: inoculating the obtained first-class seeds into a triangular fiask, culturing the amplified bacisria number for 3 days, and culturing the cultured strains as second-class seeds. The five kinds of cultivated secondary sesds are mixed with the substrate to obtain the inoculated strains, and after 5 days of culture, obtaining the mixed veast of Bacillus sublilis, Bacillus mecaterium, Bacillus rruciiaeinosus, Bacillus saccharomyces, Bacillus aspergilus oryzas.
Step ©: Making organic fertilizer, and uniformly mixing crop straw, rice bran and the mixed yeast, wherein each ton of fermentation raw materials consists of 0,8 ton of crop straw and 0.2 ton of rice bran. The dosage of mixed ysasi is 5 kg of mixed ysast per ton of crop straw. Uniformly mixing crop straws, rice bran and mixed yeast in fermentation workshop with shade and rain protection, and stacking into strips with 3 bottom width of 5-7 meters, a top width of 2.5.3.0 meters and a height of 25-20 meters, and alowing the strips to naturally ferment. Turning the pile once every 10-12 days, and naturally fermenting for 25 days to obtain the organic fertilizer A produced by this method Comparative embodiment 1 in the experiment, the common yeast in the market was used for comparison, 0500878 The common yeast dosage was 4.0 kg yeast per ton of fermented material, and the organic fertilizer produced by conventional fermentation method was obtained.
The fermented material is crop straw (without adding rice bran). The general yeast is composed of yeast and Bacillus subtilis (ACCC 11068),
The organic fertilizer A produced by this method and the organic fertilizer produced by comparative embodiment 1 were used for the planting of Ipomoea aquatica and Brassica juncea respectively, and the soit pH of Ipomoea aquatica 186.3. The usage amount of this organic fertilizer A and the organic fertiizer of comparative embodiment 1 are both 160 kalmu, and other planting methods are all planted according to the conventions! method.
Soil pH 6.2 in Brassica juncea planting area The usage amount of this organic fertilizer A and the organic fertilizer of comparative embodiment 1 are both 160 kg/mu, and other planting methods are all planted according to the conventional method,
The effect of organic fertilizer A obtained in embodiment 1 was comparsd with that obtained in comparative embodiment 1, and the results are detailed in Table 1. From the comparison of data in Table 1, it can be seen that the yield of the organic fertilizer Ipomoea aquatica produced by this method is 3411kgimu, which is 831 kg higher than the yield of Ipomoea aguatica which is 2780 kofmu with the chemical fertilizer of comparative smbodiment 1, with an increase of 22.70%. The effect of using this method to produce organic fertilizer A is very remarkable, and the organic fertilizer produced by this method can fully mest the healthy and safe vegetable standard for planting Ipomosa aquatica,
The output of the organic fertilizer Brassica juncea produced by this method is 3,774 kalmu, which is 551 kg higher than that of the chemical fertilizer of comparative embodiment 1, which is 3,213 kg/mu, an increase of 17.46%. The effect of applying the organic fertilizer À produced by this method is very remarkable, and the application of the organic fertilizer produced by this method to plant Brassica iuncea completely meets the healthy and safe vegetable standard
Table 1 Comparison of effects between organic fertilizer A produced by this 0500878 method and chemical fertilizer of Comparative embodiment 1 Ipomoea in Embodiment 1 E Embodiment 1 Brassica in Embodiment 1 oo nt ? Embodiment 1 The efficient fermentation yeast and organic fertilizer comprises the following preparation steps. Step À, screening dominant fermentation strains, and selecting Bacillus subtilis (ACCC11060), Bacillus megaterum (ACCCIOOID, Bacillus muciiaeinosus (also known as silicate baclerig), AGCC11103), Bacillus saccharomyces (ACCC20065) and Aspergillus oryzas (ACCCS3O163) were used a8 fermentation strains. These five strains were purchased from the National Culture Collection Center, and the mass perceniages were: Bacillus subtilis 20%, Bacillus megaterium 20%, Bacillus mucilgeinosus 20%, Bacillus saccharomyces 20% and Bacillus aspergilus oryzae 20%, and the total mass percentage of the five components is 100%.
Step B: Making mixed yeast, using the scresnad dominant fermentation strains for mixed yeast production, operating under sterile conditions, purifying the dominant strains, inoculating and culturing, and obtaining the strains as first-class seeds, Shaking table amplification: inoculating the obtained first-class sesds into a triangular flask, culturing the amplified bacteria number for 3-5 days, and cuituring the cultured strains as second-class seeds. The five kinds of cultivated secondary seeds are mixed with the substrate to obtain the inoculated strains, and after 5 -7 days of culture, obtaining the mixed yeast of Bacillus subtilis, Bacillus megaterium, Bacillus muciiasinosus, Bacillus saccharomyces, Bacillus aspergilus oryzas.
Step CG: Making organic fertifizer, and uniformly mixing crop straw, rice bran and the mixed yeast, wherein each ton of fermentation raw materials consists of 0.85 ton of crop straw and 0.15 ton of rice bran. The dosage of mixed yeast is 4 kg of mixed 0500878 yeast per ton of crop straw. Uniformly mixing CrOD straws, rice bran and mixed yeast in fermentation workshop with shade and rain protection, and stacking into strips with a bottom width of 5-7 metiers, a top width of 2.5.3.0 meters and a height of 2.5-3.0 meters, and allowing the strips to naturally ferment. Turning the pile once every 10-12 days, air is introduced into the fermentation reactor through an air pump and an air pipe with air holes for every TO days, and naturally fermenting for 25 days 10 obtain the organic fertilizer B produced by this method.
Comparative embodiment 2 The organic fertilizer produced by conventional method is produced by mixing common yeasi on the market with crop straw and rice bran and fermenting. The general yeast in the market consists of yeast and Bacillus subtilis (No ACCCTIOGS) in which each ton of fermentation raw materials consists of 0.8 tons of crop straw and
0.2 tons of rice bran. The dosage of mixed yeast is 5 kg of mixed yeast per ton of crop straw, The organic fertilizer B produced by this method and the organic fertiiizer produced by Comparative Embodiment 2 were used for the planting of Ipomoea acuatica and Brassica junces, respectively. The soil pH of Ipomoea aquatica planting area was 6.4, and the cadmium (Cd) content in the soil was 0. 73mg/ko, The usage amount of this organic fertilizer B and the organic fertilizer of Comparative embodiment 2 are both 150 kgfmu, and other planting methods are all planted according to the conventional method, The pH of the soil in Brassica juncea planting area is 6.3, and the content of cadmium (Cd) in the soil is D 73mg/ko. The usage amount of this organic fertilizer B and the organic fertilizer of Comparative embodiment 2 are both 150 kalmu, and other planting methods are all planted according to the conventional method.
The effect of organic fertilizer B obtained in embodiment 2 was compared with that obtained in Comparative embodiment 2, and the results are shown in Table 2 From the comparison of data in Table 2, il can be seen that the yield of the organic fertilizer water spinach produced by this method is 3504 kgfmu, which is 682 kg higher than that of the chemical fertilizer water spinach which is 2822 kg/mu, with an 0500878 increase of 24 17%. The effect of applying the organic fertilizer B produced by this method is very remarkable, and the organic fertilizer produced by this method can fully meet the healthy and safe vegatable standard for planting Ipomoea aquatica.
The vield of organic fertilizer Brassica juncea produced by this method is 3842 kalmu, which is 541 Kg higher than that of chemical fertilizer Brassica juncea which is 3301 kg/mu, an increase of 16.38%. The affect of applying the organic fertilizer B produced by this method is very remarkable, and the application of the organic fertilizer produced by this method to plant mustard completely meets the healthy and safe vegetable standard.
Table 2 Comparison of effects between organic fertilizer B produced by this method and chemical fertilizer of Comparative embodiment = Ipomoea in Embodiment 2 Embodiment 2 Brassica in Embodiment 2 Embodiment 2 Embodiment 3 The efficient fermentation yeast and organic fertilizer comprises the following preparation steps: Step À, screening dominant fermentation strains, and selecting Bacillus subtilis {ACCC11050), Bacillus megaterium (ACCC10010), Bacillus muciiasinosus {also known as silicate bacteria), ACCC11102) Bacillus saccharomyces (ACCC20085) and Aspergiilus oryzae (ACCC30163) were used as fermentation strains. Thess five strains were purchased from the National Culture Collection Center, and the mass 0500878 percentages were: Bacillus subtilis 20%, Bacillus megaterium 25%, Bacillus muciizeinosus 20%, Bacillus saccharomyces 15% and Bacillus aspergillus oryzae 20%, and the total mass percentages of the five components is 100%.
Step B Making mixed yeast using the screened dominant fermentation strains for mixed yeast production, operating under sterile conditions, purifying the dominant strains, inoculating and cultunng, and obtaining the strains as first-class seeds, Shaking table amplification: inoculating the obtained first-class seeds into a triangular flask, culturing the amplified bacteria number for 3-5 days, and culturing the cultured strains as second-class seeds. The five kinds of cultivated secondary seeds are mixed with the substrate to obtain the inoculated strains, and after 5-7 days of culture, obtaining the mixed veaast of Bacillus sublilis, Bacillus megaterium, Bacillus muciisemnosus, Bacillus saccharomyces, Bacillus aspergillus oryzas.
Step ©: Making organic fertilizer, and uniformly miking crop straw, rice bran and the mixed yeast, wherein each ton of fermentation raw materials consists of 0.82 ton of crop straw and 0.18 ton of rice bran. The dosage of mixed yeast is 3 kg of mixed yeast per ton of crop straw. Uniformly mixing crop straws, rice bran and mixed yeast in fermentation workshop with shade and rain protection, and stacking ini strips with a bottom width of 5-7 metiers, a top width of 2.5.3.0 meters and a height of 2.5-3.0 meters, and allowing the strips Lo naturally ferment. Turning the pile once every 10-12 days, air is introduced into the fermentation reactor through an air pump and an air pipe with air holes for every 12 days, and naturally fermenting for 25 days 10 obtain the organic fertilizer © produced by this method.
Comparative embodiment 3 Compared with the common yeast in the market, the amount of common yeast is
4.0 kg yeast per ion of fermented material to obtain organic fertilizer produced by conventional methods, The fermentation raw material is crop straw; The general yeast is composed of yeast, Bacillus mucilasinosus (ACCCT1103) and Bacillus subtilis {ACCC 11068) mixed in a weight ratio of 11:1.
The organic fertilizer © produced by this method and the organic fertilizer 0500878 produced by Comparative embodiemin 3 were used for the planting of Ipomosa aquatica and Brassica juncea respectively, and the soit pH of Ipomoea aguatica was
6.3. The usage amount of this organic fertilizer © and the organic fertilizer of Comparative embodiment 3 are ail 120 kg/mu, and other planting methods are all slanted according to the conventional method, Soil pH 6.3 in mustard planting ares. The usage amount of this organic fertilizer © and the organic fertilizer of Comparative embodiment à are all 120 kg/mu, and other planting methods are ali planted according to the conventional method, The effect of organic fertilizer © obtained in embodiment 2 was compared with that obtained in Comparative embodiment 3. See Table 3 for details From the comparison of data in Table 3, it can be seen that the yield of organic fertilizer water spinach produced by this method is 3,250 kg/mu, which is 747 kg higher than that of chemical fertilizer water spinach which is 2,503 kalmu, with an increase of 28.84%. The effect of applying the organic fertibzer C produced by this method is very remarkable, and the organic fertilizer produced by this method can fully meet the healthy and safe vegetable standard for planting Ipomoea aquatica, The yield of organic fertilizer Brassica juncea produced by this method is 3427 kafmu, which is 550 kg higher than that of chemical fertilizer Brassica junces which is 2877 kg/mu, an increase of 18.12%. The effect of applying the organic fertilizer C produced by this method is very remarkable, and the application of the organic fertilizer produced by this method to plant Brassica juncea compistely mests the healthy and safe vegetable standard,
LU5S00878 Table 3 Comparison table of effects of organic fertilizer © produced by this method and chemical fertilizer of Comparative Embodiment 3 Subject Organic fertilizer kind Vegetable yield (kg/mu) Compare the organic fertilizer produced 2503 Ipomoea in Embodiment 2 aquatica Organic fertilizer A produced in 3250 Embodiment 2 Compare the organic fertilizer produced 2877 Brassica in Embodiment 2 juncea Organic fertilizer À produced in 3427 Embodiment 2 To sum up, compared with general yeast fermentation, the organic fertiiizer prepared by this technical scheme has higher fertilizer efficiency, which Is conducive to crop absorption and improvement of crop yield and quality. Using waste crop straws as raw materials not only solves the environmental pollution problem caused by domestic sludge that has been bothering cities, but also turns waste into treasure, protects the environment and promotes the sustainable development of agricultural industry.
The above content is a further detailed description of the present invention combined with specific preferred embodiments, and it cannot be considered that the specific implementation of the present invention is only limited to these descriptions. For ordinary technicians in the technical field to which the present invention belongs, without departing from the concept of the present invention, several simple deductions or substitutions can be made, ail of which should be regarded as belonging to the scope of protection of the present invention.

Claims (8)

Claims LU5S00878
1. Efficient fermentation of yeast and organic fertilizer, which is characterized by being prepared by the following methods: 1, screening dominant fermentation strains including Bacillus subtilis, Bacillus megaterium, Bacillus muchaeinosus, Bacillus saccharomyces and Bacillus aspergilus aryzas, s2, making mixed yeast by purifying, inoculating, expanding and miking the dominant fermentation siraing, 83, making organic fertilizer, uniformiy mixing the crop straw, rice bran and the mixed yeast, and fermenting to obtain organic fertilizer, in which, the weight ratio of crop straw io rice oran is (16-17) (3-4), and 2-5 kg of mixed yeast is added per ton of crop straw.
2. The organic fertilizer, according to claim 1, Is characterized in that in si, the dominant fermentation strain consists of the following components in percentage by mass. 20-30% of Bacillus subtilis, 20-25% of Bacillus megaterium, 20-25% of Bacillus muciisemnosus, 15-25% of Bacillus saccharomyces and 15-20% of Bacillus aspergitius oryzae.
3 The organic fertilizer, according to claim 2, is characterized in that the dominant fermentation strain consists of the following components in percentage by mass. 20% of Bacillus subtilis, 20% of Bacillus megaisrum, 20% of Bacilius muciisemnosus, 20% of Bacillus saccharomyces and 20% of Bacillus aspergilius OTYZBE.
4. The organic fertilizer, according to claim 1, is characterized in that in 82, the preparation of veast comprises the following steps: using the dominant strains described in si for mixed yeast production, operating under sterile conditions, purifying the dominant strains, inoculating and culturing, and obtaining the strains as first-class seeds, shake-table amplification, inoculating the obtained first-class seeds into culture container, culturing the number of amplified bacteria for 3-5 days, and culturing the cultured strains as second-class seeds, mixing the cultivated five Kinds of secondary seeds with the substrate to obtain inoculated strains, and culturing for 5-7 days to obtain the mixed yeast,
5. The organic fertilizer, according to claim 4, is characterized in that in sa, the fermentation is natural fermentation, which includes the following steps: uniformly mixing crop straws, rice bran and the mixed yeast, and stacking into strips with a 0500878 bottom width of 5-7 meters, a top widih of 2.5-3.0 meters and a height of 2.5-3.0 meters, and naturally fermenting.
& The organic feriiizer, according to claim 5 is characterized in that in the natural fermentation, the compost is turned every 10-12 days, and after natural fermentation for 25-35 days, the organic fertilizer produced by the method is obtained,
7. The organic fertilizer, according to clam 5, is characterized in that during fermentation, air is introduced into the fermentation pile through the air pump and the air pipe with air holes.
8. The organic fertilizer, according to any from claim 1 to 7, is characterized in that the usage amount of the organic fertilizer is 120-160 kafmu, appiving once a year.
LU500878A 2021-11-18 2021-11-18 Efficient fermentation yeast and organic fertilizer and preparation method thereof LU500878B1 (en)

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