KR960014360A - 동물유전자를 이용한 형질전환된 식물세포 선별방법 - Google Patents
동물유전자를 이용한 형질전환된 식물세포 선별방법 Download PDFInfo
- Publication number
- KR960014360A KR960014360A KR1019940027936A KR19940027936A KR960014360A KR 960014360 A KR960014360 A KR 960014360A KR 1019940027936 A KR1019940027936 A KR 1019940027936A KR 19940027936 A KR19940027936 A KR 19940027936A KR 960014360 A KR960014360 A KR 960014360A
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- South Korea
- Prior art keywords
- transformed plant
- genes
- plant cells
- plant cell
- selection method
- Prior art date
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
- C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
- C12Y305/04004—Adenosine deaminase (3.5.4.4)
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Cell Biology (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
이 발명은 특수한 성능을 가진 유전자가 도입된 형질 전환된 식물 세포를 선별함에 있어서, 미생물 유래 표지 유전자를 사용하는데 따른 인체 안정성 문제와 여러 종류의 선별 표지 유전자를 사용하여야 하는 문제점을 해결하기 위한 것임.
본 발명은 동물 유전자를 이용하여 형질 전환된 식물 세포를 선별하는 방법으로 구성됨. 동물 유전자로는 아라-에이, 코디세핀, 자일-에이, 2-디옥시아데노신등을 포함하는 아데노신 탈아민 효소 유전자를 사용함.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
Claims (3)
- 아데노신 탈아민효소 유전자를 형질전환된 식물세포의 선별표지로 사용하는 방법.
- 형질전환된 식물세포를 세포독성을 갖는 아데노신 유도체가 함유된 배지에서 배양하여 형질전환된 식물세포를 선별하는 방법.
- 청구범위 2항에서, 세포독성을 갖는 아데노신 유도체가 아라-에이, 코디세핀, 자일-에이, 2-디옥시아데노신중에서 선택된 것임을 특징으로 하는 방법.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1019940027936A KR0134274B1 (ko) | 1994-10-28 | 1994-10-28 | 동물유전자를 이용한 형질전환된 식물세포 선별방법 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1019940027936A KR0134274B1 (ko) | 1994-10-28 | 1994-10-28 | 동물유전자를 이용한 형질전환된 식물세포 선별방법 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR960014360A true KR960014360A (ko) | 1996-05-22 |
| KR0134274B1 KR0134274B1 (ko) | 1998-04-20 |
Family
ID=19396351
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1019940027936A KR0134274B1 (ko) | 1994-10-28 | 1994-10-28 | 동물유전자를 이용한 형질전환된 식물세포 선별방법 |
Country Status (1)
| Country | Link |
|---|---|
| KR (1) | KR0134274B1 (ko) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR19980026528A (ko) * | 1996-10-10 | 1998-07-15 | 성재갑 | 다용도 세정제 조성물 |
| KR20000039604A (ko) * | 1998-12-15 | 2000-07-05 | 성재갑 | 소취기능 고형 변기세정제 조성물 |
-
1994
- 1994-10-28 KR KR1019940027936A patent/KR0134274B1/ko not_active IP Right Cessation
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR19980026528A (ko) * | 1996-10-10 | 1998-07-15 | 성재갑 | 다용도 세정제 조성물 |
| KR20000039604A (ko) * | 1998-12-15 | 2000-07-05 | 성재갑 | 소취기능 고형 변기세정제 조성물 |
Also Published As
| Publication number | Publication date |
|---|---|
| KR0134274B1 (ko) | 1998-04-20 |
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