WO1998049277A3 - Verfahren zur erzeugung eines endoinulinase produzierenden mikroorganismus und verfahren zur detektion von endoinulinase aktivität - Google Patents

Verfahren zur erzeugung eines endoinulinase produzierenden mikroorganismus und verfahren zur detektion von endoinulinase aktivität Download PDF

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Publication number
WO1998049277A3
WO1998049277A3 PCT/EP1998/002426 EP9802426W WO9849277A3 WO 1998049277 A3 WO1998049277 A3 WO 1998049277A3 EP 9802426 W EP9802426 W EP 9802426W WO 9849277 A3 WO9849277 A3 WO 9849277A3
Authority
WO
WIPO (PCT)
Prior art keywords
endoinulinase
inulin
activity
obtaining
detecting
Prior art date
Application number
PCT/EP1998/002426
Other languages
English (en)
French (fr)
Other versions
WO1998049277A2 (de
Inventor
Barbara I Tshisuaka
Original Assignee
Barbara I Tshisuaka
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Barbara I Tshisuaka filed Critical Barbara I Tshisuaka
Publication of WO1998049277A2 publication Critical patent/WO1998049277A2/de
Publication of WO1998049277A3 publication Critical patent/WO1998049277A3/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01007—Inulinase (3.2.1.7)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Die vorliegende Erfindung betrifft ein Verfahren zur Erzeugung eines Endoinulinase produzierenden Mikroorganismus. Erfindungsgemäß sind folgende Verfahrensschritte vorgesehen: Sammeln von Erdproben und/oder Gewebeproben aus dem Wurzelbereich inulinspeichernder Pflanzen, Selektion von Mikroorganismen, die Inulin als einzige Kohlenstoffquelle nutzen, durch Zufügen von inulinhaltigem Medium über einen definierten Zeitraum, Vereinzeln und Kultivieren der selektierten Mikroorganismen. Die vorliegende Erfindung betrifft ferner einen Mikroorganismus mit reiner Endoinulinase-Aktivität sowie ein Verfahren zur Detektion von Inulinase-Aktivität mit folgenden erfindungsgemäßen Verfahrensschritten: Markieren von Inulin mit einer Markierungssubstanz, Inkubation des markierten Inulins mit Inulinase über einen definierten Zeitraum, Abtrennung des nicht umgesetzten markierten Inulins, Bestimmung der Extinktion des Ansatzes bei 592 nm.
PCT/EP1998/002426 1997-04-26 1998-04-24 Verfahren zur erzeugung eines endoinulinase produzierenden mikroorganismus und verfahren zur detektion von endoinulinase aktivität WO1998049277A2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19717823.5 1997-04-26
DE1997117823 DE19717823A1 (de) 1997-04-26 1997-04-26 Verfahren zur Erzeugung eines Endoinulinase produzierenden Mikroorganismus, Verfahren zur Herstellung von Endoinulinase sowie Verfahren zur Herstellung von Oligofructosiden aus Insulin und Verfahren zur Detektion von Endoinulinase-Aktivität

Publications (2)

Publication Number Publication Date
WO1998049277A2 WO1998049277A2 (de) 1998-11-05
WO1998049277A3 true WO1998049277A3 (de) 1999-03-04

Family

ID=7827934

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1998/002426 WO1998049277A2 (de) 1997-04-26 1998-04-24 Verfahren zur erzeugung eines endoinulinase produzierenden mikroorganismus und verfahren zur detektion von endoinulinase aktivität

Country Status (2)

Country Link
DE (1) DE19717823A1 (de)
WO (1) WO1998049277A2 (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6042605A (en) 1995-12-14 2000-03-28 Gore Enterprose Holdings, Inc. Kink resistant stent-graft
CN103642738B (zh) * 2013-12-13 2015-03-18 大连民族学院 一株产内切菊粉酶的灰平链霉菌s501 及其培养方法和应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62208277A (ja) * 1986-03-07 1987-09-12 Meiji Seika Kaisha Ltd イヌリナ−ゼの製造法
JPS62228293A (ja) * 1986-03-28 1987-10-07 Mitsui Toatsu Chem Inc エンド型イヌリナーゼおよびその産生方法
JPH03198774A (ja) * 1989-12-28 1991-08-29 Mitsui Toatsu Chem Inc イヌリナーゼの製造法
JPH04190789A (ja) * 1990-11-27 1992-07-09 Mitsui Toatsu Chem Inc イヌリナーゼの製造法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4865852A (en) * 1987-06-05 1989-09-12 Mitsui Toatsu Chemicals, Inc. Additive for stock feeds, stock feed containing additive, and process for preparation of additive

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62208277A (ja) * 1986-03-07 1987-09-12 Meiji Seika Kaisha Ltd イヌリナ−ゼの製造法
JPS62228293A (ja) * 1986-03-28 1987-10-07 Mitsui Toatsu Chem Inc エンド型イヌリナーゼおよびその産生方法
JPH03198774A (ja) * 1989-12-28 1991-08-29 Mitsui Toatsu Chem Inc イヌリナーゼの製造法
JPH04190789A (ja) * 1990-11-27 1992-07-09 Mitsui Toatsu Chem Inc イヌリナーゼの製造法

Non-Patent Citations (15)

* Cited by examiner, † Cited by third party
Title
AGRICULTURAL CHEMISTRY AND BIOTECHNOLOGY 39 (2). 1996. 99-103. ISSN: 0368-2897 *
BIOLOGICAL ABSTRACTS, vol. 96, Philadelphia, PA, US; abstract no. 379426, KIM K Y ET AL: "Production of a novel endo-inulinase from Arthrobacter sp. S37." XP002084647 *
CHEMICAL ABSTRACTS, vol. 116, no. 17, 27 April 1992, Columbus, Ohio, US; abstract no. 169675y, ZEMEK JIRI ET AL: "Medium for screening microorganisms for hydrolase activity" page 418; column r; XP002084648 *
CHEMICAL ABSTRACTS, vol. 118, no. 11, 15 March 1993, Columbus, Ohio, US; abstract no. 97152s, ZEMEK JIRI ET AL: "Preparation of a chromogenic substrate for assay of inulinase" page 363; column l; XP002084650 *
CHEMICAL ABSTRACTS, vol. 124, no. 19, 6 May 1996, Columbus, Ohio, US; abstract no. 258575y, V. POORNA ET AL: "screening for inulinase producers" page 1055; column l; XP002084649 *
G.R. CASTRO ET AL: "A plate technique for screening of inulin degrading microorganisms", JOURNAL OF MICROBIOLOGICAL METHODS, vol. 22, no. 1, 1995, pages 51 - 56, XP002084642 *
LEE SUN-HEE ET AL: "Screening of the endoinulinase-producing fungi by using antibody", KOREAN JOURNAL OF APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 21, no. 1, 1993, pages 18 - 22, XP002084645 *
PATENT ABSTRACTS OF JAPAN vol. 012, no. 070 (C - 479) 4 March 1988 (1988-03-04) *
PATENT ABSTRACTS OF JAPAN vol. 012, no. 096 (C - 484) 29 March 1988 (1988-03-29) *
PATENT ABSTRACTS OF JAPAN vol. 015, no. 467 (C - 0888) 27 November 1991 (1991-11-27) *
PATENT ABSTRACTS OF JAPAN vol. 016, no. 512 (C - 0998) 22 October 1992 (1992-10-22) *
S. ONODORA ET AL: "Molecular cloning and nucleotide sequences of cDNA and gene encoding endo-inulinase from Penicillium purpurogenum", BIOSCIENCE BIOTECHNOLOGY BIOCHEMISTRY., vol. 60, no. 11, 1996, TOKYO JP, pages 1780 - 1785, XP002084644 *
S. ONODORA ET AL: "Purification and substrate specificity of endo-type inulinase from Penicillium purpurogenum", AGRICULTURAL AND BIOLOGICAL CHEMISTRY., vol. 52, no. 10, 1988, TOKYO JP, pages 2569 - 2576, XP002084643 *
TOYOHIKO NAKAMURA ET AL: "Production, purification and properties of an endoinulinase of Penicillium s. TN-88 that liberates inulotriose", JOURNAL OF FERMENTATION AND BIOENGINEERING, vol. 84, no. 4, July 1997 (1997-07-01), pages 313 - 318, XP002084646 *
TRENDS CARBOHDR. CHEM., CARBOHYDR. CONF., 9TH 1993, 1995, pages 195 - 199 *

Also Published As

Publication number Publication date
DE19717823A1 (de) 1998-10-29
WO1998049277A2 (de) 1998-11-05

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