WO1998002556A3 - Screening for and use of an esterase for a stereospecific resolution - Google Patents

Screening for and use of an esterase for a stereospecific resolution Download PDF

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Publication number
WO1998002556A3
WO1998002556A3 PCT/GB1997/001909 GB9701909W WO9802556A3 WO 1998002556 A3 WO1998002556 A3 WO 1998002556A3 GB 9701909 W GB9701909 W GB 9701909W WO 9802556 A3 WO9802556 A3 WO 9802556A3
Authority
WO
WIPO (PCT)
Prior art keywords
screening
esterase
resolution
compounds
formula
Prior art date
Application number
PCT/GB1997/001909
Other languages
French (fr)
Other versions
WO1998002556A2 (en
Inventor
Linden Gledhill
Christopher Martin Kell
Original Assignee
Smithkline Beecham Plc
Linden Gledhill
Christopher Martin Kell
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9614809.3A external-priority patent/GB9614809D0/en
Priority claimed from GBGB9700082.2A external-priority patent/GB9700082D0/en
Application filed by Smithkline Beecham Plc, Linden Gledhill, Christopher Martin Kell filed Critical Smithkline Beecham Plc
Priority to JP10505751A priority Critical patent/JP2000514653A/en
Priority to EP97931929A priority patent/EP0937152A2/en
Publication of WO1998002556A2 publication Critical patent/WO1998002556A2/en
Publication of WO1998002556A3 publication Critical patent/WO1998002556A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/10—Nitrogen as only ring hetero atom
    • C12P17/12—Nitrogen as only ring hetero atom containing a six-membered hetero ring
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P41/00—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture
    • C12P41/003—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by ester formation, lactone formation or the inverse reactions
    • C12P41/005—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by ester formation, lactone formation or the inverse reactions by esterification of carboxylic acid groups in the enantiomers or the inverse reaction

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Plural Heterocyclic Compounds (AREA)

Abstract

A method of screening for microorganisms capable of the resolution of compounds of formula (I), which method comprises selecting for organisms capable of using compounds of formula (I) as a sole carbon source and assaying cells of the organism for specific esterase activity, can be used to identify enzymes useful in the manufacture of paroxetine.
PCT/GB1997/001909 1996-07-15 1997-07-14 Screening for and use of an esterase for a stereospecific resolution WO1998002556A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP10505751A JP2000514653A (en) 1996-07-15 1997-07-14 Screening of esterases for stereospecific resolution and its use
EP97931929A EP0937152A2 (en) 1996-07-15 1997-07-14 Screening for and use of an esterase for a stereospecific resolution

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB9614809.3 1996-07-15
GBGB9614809.3A GB9614809D0 (en) 1996-07-15 1996-07-15 Novel compounds
GBGB9700082.2A GB9700082D0 (en) 1997-01-03 1997-01-03 Novel bioprocess
GB9700082.2 1997-01-03

Publications (2)

Publication Number Publication Date
WO1998002556A2 WO1998002556A2 (en) 1998-01-22
WO1998002556A3 true WO1998002556A3 (en) 1998-04-23

Family

ID=26309700

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1997/001909 WO1998002556A2 (en) 1996-07-15 1997-07-14 Screening for and use of an esterase for a stereospecific resolution

Country Status (3)

Country Link
EP (1) EP0937152A2 (en)
JP (1) JP2000514653A (en)
WO (1) WO1998002556A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2161167B1 (en) * 1999-09-24 2002-07-01 Vita Invest Sa Enzymatic hydrolysis for paroxetine synthesis intermediates production consists of enantio selective hydrolysis of a racemic ester based mixture
GB9924882D0 (en) * 1999-10-20 1999-12-22 Smithkline Beecham Plc Novel process
AU2001238407A1 (en) * 2000-02-16 2001-08-27 Thermogen, Inc. Esterase enzymes having selective activity

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0223403A2 (en) * 1985-10-25 1987-05-27 Beecham Group Plc Piperidine derivative, its preparation, and its use as medicament
WO1993022284A1 (en) * 1992-05-06 1993-11-11 Smithkline Beecham Plc Process for stereospecific hydrolysis of piperidinedione derivatives
WO1994003428A1 (en) * 1992-08-06 1994-02-17 Sepracor, Inc. Method of preparing optically pure precursors of paroxetine
GB2297550A (en) * 1995-02-06 1996-08-07 Smithkline Beecham Plc Forms of paroxetine hydrochloride
WO1996024595A1 (en) * 1995-02-06 1996-08-15 Smithkline Beecham Plc New forms of paroxetin hydrochloride

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0223403A2 (en) * 1985-10-25 1987-05-27 Beecham Group Plc Piperidine derivative, its preparation, and its use as medicament
US4721723A (en) * 1985-10-25 1988-01-26 Beecham Group P.L.C. Anti-depressant crystalline paroxetine hydrochloride hemihydrate
WO1993022284A1 (en) * 1992-05-06 1993-11-11 Smithkline Beecham Plc Process for stereospecific hydrolysis of piperidinedione derivatives
WO1994003428A1 (en) * 1992-08-06 1994-02-17 Sepracor, Inc. Method of preparing optically pure precursors of paroxetine
GB2297550A (en) * 1995-02-06 1996-08-07 Smithkline Beecham Plc Forms of paroxetine hydrochloride
WO1996024595A1 (en) * 1995-02-06 1996-08-15 Smithkline Beecham Plc New forms of paroxetin hydrochloride

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
BIOCHEM. J. (1995), 307(3), 735-41 CODEN: BIJOAK;ISSN: 0264-6021 *
CHEMICAL ABSTRACTS, vol. 121, no. 9, 29 August 1994, Columbus, Ohio, US; abstract no. 102935, BLAKE, C. C. F. ET AL: "The active site of methanol dehydrogenase contains a disulfide bridge between adjacent cysteine residues" XP002057142 *
CHEMICAL ABSTRACTS, vol. 123, no. 7, 14 August 1995, Columbus, Ohio, US; abstract no. 78380, AVEZOUX, ALAIN ET AL: "The role of the novel disulfide ring in the active site on the quinoprotein methanol dehydrogenase from Methylobacterium extorquens" XP002057141 *
KOHNO, MITSUTAKA ET AL: "The crystal structure of lipase II from Rhizopus niveus at 2.2.ANG. resolution", J. BIOCHEM. (TOKYO) (1996), 120(3), 505-510 CODEN: JOBIAO;ISSN: 0021-924X, XP002057140 *
NAT. STRUCT. BIOL. (1994), 1(2), 102-5 CODEN: NSBIEW;ISSN: 1072-8368 *
OLLIS, DAVID L. ET AL: "The.alpha./.beta. hydrolase fold", PROTEIN ENG. (1992), 5(3), 197-211 CODEN: PRENE9;ISSN: 0269-2139, XP002057138 *
PARENTE, AUGUSTO ET AL: "Molecular basis of superreactivity of cysteine residues 31 and 32 of seminal ribonuclease", BIOCHEMISTRY (1985), 24(5), 1098-104 CODEN: BICHAW;ISSN: 0006-2960, XP002057139 *
SCHRAG, JOSEPH D. ET AL: "Pancreatic lipases: evolutionary intermediates in a positional change of catalytic carboxylates?", J. BIOL. CHEM. (1992), 267(7), 4300-3 CODEN: JBCHA3;ISSN: 0021-9258, XP002057137 *
SCHRAG, JOSEPH D. ET AL: "Redesigning the active site of Geotrichum candidum lipase", PROTEIN ENG. (1995), 8(8), 835-42 CODEN: PRENE9;ISSN: 0269-2139, XP002057136 *

Also Published As

Publication number Publication date
JP2000514653A (en) 2000-11-07
WO1998002556A2 (en) 1998-01-22
EP0937152A2 (en) 1999-08-25

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