KR960010019A - 단일클론 항체가 결합된 라텍스의 제조방법 - Google Patents

단일클론 항체가 결합된 라텍스의 제조방법 Download PDF

Info

Publication number
KR960010019A
KR960010019A KR1019940022352A KR19940022352A KR960010019A KR 960010019 A KR960010019 A KR 960010019A KR 1019940022352 A KR1019940022352 A KR 1019940022352A KR 19940022352 A KR19940022352 A KR 19940022352A KR 960010019 A KR960010019 A KR 960010019A
Authority
KR
South Korea
Prior art keywords
monoclonal antibody
latex
villi
gonadotropin
stimulating hormone
Prior art date
Application number
KR1019940022352A
Other languages
English (en)
Other versions
KR0134292B1 (ko
Inventor
이종욱
고인영
전재현
차정학
장진동
Original Assignee
김태훈
주식회사 유한양행
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 김태훈, 주식회사 유한양행 filed Critical 김태훈
Priority to KR1019940022352A priority Critical patent/KR0134292B1/ko
Publication of KR960010019A publication Critical patent/KR960010019A/ko
Application granted granted Critical
Publication of KR0134292B1 publication Critical patent/KR0134292B1/ko

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/22—Hormones
    • A61K38/24—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g. HCG; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/56—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule
    • A61K47/58—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. poly[meth]acrylate, polyacrylamide, polystyrene, polyvinylpyrrolidone, polyvinylalcohol or polystyrene sulfonic acid resin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
    • G01N33/545—Synthetic resin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Public Health (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Hematology (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Endocrinology (AREA)
  • Veterinary Medicine (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Reproductive Health (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

본 발명은 항-융모 성선 자극 호르몬 -단일클론 항체 (ANTI-HUMAN CHRIONIC GONADOTROPIN-MONOCLONAL ANTIBODY)와 라텍스를 접촉시켜 항-융모성선자극 호르몬-단일클론 항체가 결합된 라텍스를 제조하는 방법을 제공한다. 본 발명의 방법에 따르면, 항-융모성선자극호르몬-단일클론 항체와 소혈철알부민을 1:5내지 1:40중량비의비율로 혼합하고 이 혼합물에 라택스를 가야여 반응시키므로써 항-융모성선자극 호르몬-단일클론 항체가 결합된 라텍스를 제조한다. 본발명의 방법에 의하여 제조된 단일클론 항체 결합 라택스는 항원결합성 또는 민감도가 향상 되었기 때문에 고상 면역 크로마토 그래피를 이용한 임신진단에 유용하게 이용될 수 있다.

Description

단일클론 항체가 결합된 라텍스의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (4)

  1. 항-융모성선자극호르몬-단일클론 항체(anti-Human Chrionic Gonadotropin -Monoclonal antibody) 와 라텍스를 접촉시켜 항-융모성선자극 호르몬-단일클론 항체가 결합된 라텍스를 제조하는 방법에 있어서, 항-융모성선자극호르몬-단일클론 항체와 소혈청 알부민을 혼합하고, 이 혼합물에 라텍스를 가하여 반응시키는 것을 특징으로 하는 항-융모성선자극호르몬-단일클론 항체가 결합된 라텍스의 제조방법.
  2. 제 1항에 있어서, 소혈청 알부민과 항-융모성선자극 호르몬-단일클론 항체의 비율은 1:5-40중량비임을 특징으로 하는 항-융모성선자극 호르몬-단일클론 항체가 결합된 라텍스의 제조방법.
  3. 제1항에 있어서, 라텍스가 폴리스티렌인것을 특징으로 하는 항-융모성선자극호르몬-단일클론 항체가 결합된 라텍스의 제조방법.
  4. 제 1항 또는 제 3항에 있어서, 라텍스의 입자크기가 0.05-0.5μm인것을 특징으로 하는 항-융모성선자극 호르몬-단일클론 항체가 결합된 라텍스의 제조 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019940022352A 1994-09-06 1994-09-06 단일클론 항체가 결합된 라텍스의 제조방법 KR0134292B1 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019940022352A KR0134292B1 (ko) 1994-09-06 1994-09-06 단일클론 항체가 결합된 라텍스의 제조방법

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019940022352A KR0134292B1 (ko) 1994-09-06 1994-09-06 단일클론 항체가 결합된 라텍스의 제조방법

Publications (2)

Publication Number Publication Date
KR960010019A true KR960010019A (ko) 1996-04-20
KR0134292B1 KR0134292B1 (ko) 1998-04-21

Family

ID=19392105

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019940022352A KR0134292B1 (ko) 1994-09-06 1994-09-06 단일클론 항체가 결합된 라텍스의 제조방법

Country Status (1)

Country Link
KR (1) KR0134292B1 (ko)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100257507B1 (ko) * 1997-12-30 2000-07-01 김선진 단일클론항체의 등전점에 따른 항체 결합 라텍스 제조 방법

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100257507B1 (ko) * 1997-12-30 2000-07-01 김선진 단일클론항체의 등전점에 따른 항체 결합 라텍스 제조 방법

Also Published As

Publication number Publication date
KR0134292B1 (ko) 1998-04-21

Similar Documents

Publication Publication Date Title
HK1002814A1 (en) Specific antibodies to troponin t their production and their use in a reagent for the determination of heart muscle necroses
KR920701471A (ko) 면역크로마토그래피 분석 및 이를 이용하는 방법
EP0375422A3 (en) Composition and methods for determining the presence of amphetamines in a sample suspected of containing amphetamine and/or methamphetamine
DE58902586D1 (de) Immunoassay und -rolymere igg-derivate zur kompensation von stoerfaktoren in immunoassays.
CA2348379A1 (en) Immunoassay for measuring human c-peptide and kit therefor
FI90799C (fi) Immunologisia määritysmenetelmiä ja niissä käytettävä reagenssi
ES2171414T3 (es) Inmunoensayos para anticuerpos anti-hcv utilizando antigenos con epitopos conformacionales.
AU619183B2 (en) A one-step immunoassay for the determination of antigen-specific antibodies of one of the immunoglobulin classes a,m,d or e, and an agent suitable for this purpose
MX9401112A (es) Preparacion de conjugados de fotoproteina y metodos para utilizar los mismos.
KR900002075A (ko) 특이적으로 결합가능한 물질의 정량 방법 및 시약
KR920006005A (ko) 인체 IgE에 대한 모노클론 항체
JPS6428560A (en) Immunometric measurement
KR960010019A (ko) 단일클론 항체가 결합된 라텍스의 제조방법
DK199783A (da) Immunoglobulin-halvmolekyler og fremgangsmaade til fremstilling af hybrid-antistoffer
ES2061881T3 (es) Procedimiento para la determinacion de anticuerpos especificos de la clase de un antigeno asi como un reactivo apropiado para ello.
AU2003248619A1 (en) Method for the elimination of interferences in immunochromatographic assays
ES2131279T3 (es) Inmunoensayo de tipo sandwich para n-peptido.
ES2084581T3 (es) Inmunoensayos que utilizan antigenos producidos en organismos hetereologicos.
GB2214295B (en) "hapten determination method, use and components"
KR910006728A (ko) 분석물의 검출 방법
ATE40475T1 (de) Verbesserter immunoassay zur bestimmung von antikoerpern gegen native-dna.
EP0186371A3 (en) Monoclonal antibodies specific to antigens of hepatitis b virus
ES2076416T3 (es) Realizacion de un inmunoensayo segun el principio de la aglutinacion.
EP0309992A3 (en) Iron colloid-labeled antibody, preparation thereof and histochemical detection thereby
KR910021586A (ko) 리간드의 면역학적 검출방법

Legal Events

Date Code Title Description
A201 Request for examination
E701 Decision to grant or registration of patent right
GRNT Written decision to grant
FPAY Annual fee payment

Payment date: 20120106

Year of fee payment: 16

FPAY Annual fee payment

Payment date: 20130109

Year of fee payment: 17

EXPY Expiration of term