MX9401112A - Preparacion de conjugados de fotoproteina y metodos para utilizar los mismos. - Google Patents
Preparacion de conjugados de fotoproteina y metodos para utilizar los mismos.Info
- Publication number
- MX9401112A MX9401112A MX9401112A MX9401112A MX9401112A MX 9401112 A MX9401112 A MX 9401112A MX 9401112 A MX9401112 A MX 9401112A MX 9401112 A MX9401112 A MX 9401112A MX 9401112 A MX9401112 A MX 9401112A
- Authority
- MX
- Mexico
- Prior art keywords
- conjugates
- methods
- present
- preparation
- photoprotein
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
La presente invención abarca un método para la síntesis de conjugados de fotoproteínas que retienen toda o una porción considerable de la actividad luminiscente de las fotoproteínas no derivatizadas. De conformidad con la presente invención, las fotoproteínas pueden conjugarse con una variedad de enlaces o uniones que incluyen estreptavidina/avidina, glicoproteínas, lectinas, hormonas, antígenos, fármacos, anticuerpos y fragmentos de unión de antígeno, de los mismos, o cualquier otro reactivo selectivamente ligable, por medios de reticulación química. La presente invención también abarca conjugados producidos por este método, y los métodos de uso de esos conjugados.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US1711693A | 1993-02-12 | 1993-02-12 |
Publications (1)
Publication Number | Publication Date |
---|---|
MX9401112A true MX9401112A (es) | 1994-08-31 |
Family
ID=21780817
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX9401112A MX9401112A (es) | 1993-02-12 | 1994-02-11 | Preparacion de conjugados de fotoproteina y metodos para utilizar los mismos. |
Country Status (9)
Country | Link |
---|---|
US (1) | US5486455A (es) |
EP (1) | EP0683822B1 (es) |
JP (1) | JPH08506897A (es) |
AT (1) | ATE217086T1 (es) |
AU (1) | AU6171894A (es) |
DE (1) | DE69430523T2 (es) |
IL (1) | IL108607A (es) |
MX (1) | MX9401112A (es) |
WO (1) | WO1994018342A1 (es) |
Families Citing this family (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE4438660A1 (de) * | 1994-10-28 | 1996-05-02 | Boehringer Mannheim Gmbh | Biolumineszenzmarkierte Hapten-Konjugate für den Einsatz in kompetitiven Immunoassays |
EP0736770A3 (fr) * | 1995-04-05 | 1997-05-02 | Anda Biolog Sa | Conjugué immunoréactif, procédé d'obtention de ce conjugué, anticorps dirigés contre ledit conjugué, composition pharmaceutique et dispositif de diagnostic les comprenant |
DE19537952A1 (de) | 1995-10-12 | 1997-04-17 | Boehringer Mannheim Gmbh | Verfahren zum Nachweis eines Analyten |
US5876995A (en) | 1996-02-06 | 1999-03-02 | Bryan; Bruce | Bioluminescent novelty items |
US5942407A (en) * | 1996-06-25 | 1999-08-24 | Immunomatrix, Inc. | Light-emitting immunoassay |
US6416960B1 (en) | 1996-08-08 | 2002-07-09 | Prolume, Ltd. | Detection and visualization of neoplastic tissues and other tissues |
ATE290205T1 (de) | 1996-12-12 | 2005-03-15 | Prolume Ltd | Vorrichtung und verfahren zum nachweis und identifizieren von infektiösen wirkstoffen |
DE19652576A1 (de) * | 1996-12-17 | 1998-06-25 | Boehringer Mannheim Gmbh | Niedermolekulare Photoprotein-Konjugate |
US5876935A (en) * | 1997-01-08 | 1999-03-02 | Dade Behring Inc. | Luminescent specific binding assay |
CA2281648A1 (en) | 1997-02-13 | 1998-08-20 | Memorial Sloan-Kettering Cancer Center | Hybrid molecules for optically detecting changes in cellular microenvironments |
JP2001514741A (ja) * | 1997-02-18 | 2001-09-11 | オンカー インク. | 緑色蛍光蛋白質およびその蛍光変異体による標的の検出 |
DE19719465A1 (de) * | 1997-05-07 | 1998-11-12 | Boehringer Mannheim Gmbh | Konservierung von Aequorin-Reagenzien |
JP2002507410A (ja) | 1998-03-27 | 2002-03-12 | プロルーム・リミテッド | ルシフェラーゼ、蛍光タンパク質、ルシフェラーゼおよび蛍光タンパク質をコードする核酸および、診断、高処理スクリーニングおよび新規アイテムにおけるその使用 |
EP1925320A3 (en) | 1998-03-27 | 2008-09-03 | Prolume, Ltd. | Luciferases, fluorescent proteins, nucleic acids encoding the luciferases and fluorescent proteins and the use thereof in diagnostics |
ATE356355T1 (de) * | 1999-06-18 | 2007-03-15 | Cardiogenics Inc | Verfahren zur prozessführung chemilumineszenter bindungstests |
US7109315B2 (en) * | 2000-03-15 | 2006-09-19 | Bruce J. Bryan | Renilla reniformis fluorescent proteins, nucleic acids encoding the fluorescent proteins and the use thereof in diagnostics, high throughput screening and novelty items |
US7659078B1 (en) * | 2002-07-17 | 2010-02-09 | University Of Kentucky Research Foundation | Method and kit for determination of prostacyclin in plasma |
US20080280310A1 (en) * | 2007-05-09 | 2008-11-13 | Louis Panagopoulos | Testing for Blood Group Immunological Reaction Without the Use of Anti-Human Globulin |
Family Cites Families (28)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4604364A (en) * | 1974-01-04 | 1986-08-05 | Kosak Kenneth M | Bioluminescent tracer composition and method of use in immunoassays |
GB1578275A (en) * | 1977-06-14 | 1980-11-05 | Maier C | Procedure for the assay of pharmacologically immunologically and biochemically active compounds in biological fluids |
US4396579A (en) * | 1981-08-06 | 1983-08-02 | Miles Laboratories, Inc. | Luminescence detection device |
US4608246A (en) * | 1983-03-10 | 1986-08-26 | Immucor, Inc. | Testing for a blood group immunological reaction |
ES8607563A1 (es) * | 1983-10-13 | 1986-06-01 | Univ Georgia Res Found | Un metodo para detectar una interaccion ligante |
US4665022A (en) * | 1984-02-17 | 1987-05-12 | The Regents Of The University Of California | Bioluminescent assay reagent and method |
US4927916A (en) * | 1984-04-23 | 1990-05-22 | The General Hospital Corporation | Method of producing fibrin-specific monoclonal antibodies lacking fibrinogen-cross-reactivity using fibrin-specific peptides |
US4764368A (en) * | 1984-08-29 | 1988-08-16 | Dana-Farber Cancer Institute, Inc. | Acid-cleavable compound |
US4657853A (en) * | 1984-09-14 | 1987-04-14 | E. I. Du Pont De Nemours And Company | Immunoassays utilizing covalent conjugates of polymerized enzyme and antibody |
US4935339A (en) * | 1985-05-07 | 1990-06-19 | Nichols Institute Diagnostics | Delayed solid phase immunologic assay |
DE3537877A1 (de) * | 1985-10-24 | 1987-04-30 | Geiger Reinhard | Luciferin-derivate und immunoassays unter einsatz derartiger luciferin-derivate |
GB8603537D0 (en) * | 1986-02-13 | 1986-03-19 | Parker D | Conjugate compound |
US4916070A (en) * | 1986-04-14 | 1990-04-10 | The General Hospital Corporation | Fibrin-specific antibodies and method of screening for the antibodies |
DE3714147A1 (de) * | 1987-04-28 | 1988-11-17 | Boehringer Mannheim Gmbh | Immunchemisches verfahren und reagenz zur bestimmung eines polyvalenten antigens in einer fluessigen probe |
JPS6447379A (en) * | 1987-08-19 | 1989-02-21 | Chisso Corp | Preparation of calcium-dependent oxygenation enzyme |
US4981979A (en) * | 1987-09-10 | 1991-01-01 | Neorx Corporation | Immunoconjugates joined by thioether bonds having reduced toxicity and improved selectivity |
US5094939A (en) * | 1988-07-19 | 1992-03-10 | Fujirebio, Inc. | Chemiluminescence assays using stabilized dioxetane derivatives |
US4925648A (en) * | 1988-07-29 | 1990-05-15 | Immunomedics, Inc. | Detection and treatment of infectious and inflammatory lesions |
US5112615A (en) * | 1988-08-03 | 1992-05-12 | New England Deaconess Hospital Corporation | Soluble hirudin conjugates |
US5019393A (en) * | 1988-08-03 | 1991-05-28 | New England Deaconess Hospital Corporation | Biocompatible substance with thromboresistance |
US5135736A (en) * | 1988-08-15 | 1992-08-04 | Neorx Corporation | Covalently-linked complexes and methods for enhanced cytotoxicity and imaging |
US5066789A (en) * | 1988-09-30 | 1991-11-19 | Neorx Corporation | Targeting substance-diagnostic/therapeutic agent conjugates having Schiff base linkages |
JPH02253162A (ja) * | 1989-03-27 | 1990-10-11 | Chisso Corp | 特異的結合能を有する物質と結合したエクオリンを用いる検出法 |
KR910700242A (ko) * | 1988-12-22 | 1991-03-14 | 마틴 골드스타인 | 간섭 결합제 및 그 방법 |
US5134071A (en) * | 1989-02-06 | 1992-07-28 | State University Of New York | Polymerization and copolymerization of proteins |
JPH03153699A (ja) * | 1989-11-09 | 1991-07-01 | Chisso Corp | 化学結合法により担体に固定化されたエクオリン活性を示す蛋白質 |
JPH03169897A (ja) * | 1989-11-29 | 1991-07-23 | Chisso Corp | 化学修飾エクオリンとその調製法 |
US5116944A (en) * | 1989-12-29 | 1992-05-26 | Neorx Corporation | Conjugates having improved characteristics for in vivo administration |
-
1994
- 1994-02-04 DE DE69430523T patent/DE69430523T2/de not_active Expired - Fee Related
- 1994-02-04 AU AU61718/94A patent/AU6171894A/en not_active Abandoned
- 1994-02-04 EP EP94908734A patent/EP0683822B1/en not_active Expired - Lifetime
- 1994-02-04 JP JP6518303A patent/JPH08506897A/ja active Pending
- 1994-02-04 WO PCT/US1994/001387 patent/WO1994018342A1/en active IP Right Grant
- 1994-02-04 AT AT94908734T patent/ATE217086T1/de active
- 1994-02-09 IL IL10860794A patent/IL108607A/en not_active IP Right Cessation
- 1994-02-11 MX MX9401112A patent/MX9401112A/es unknown
- 1994-08-22 US US08/293,648 patent/US5486455A/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
EP0683822A4 (en) | 1997-11-05 |
DE69430523T2 (de) | 2002-11-28 |
JPH08506897A (ja) | 1996-07-23 |
DE69430523D1 (de) | 2002-06-06 |
IL108607A (en) | 1998-12-06 |
IL108607A0 (en) | 1994-05-30 |
EP0683822B1 (en) | 2002-05-02 |
ATE217086T1 (de) | 2002-05-15 |
US5486455A (en) | 1996-01-23 |
AU6171894A (en) | 1994-08-29 |
EP0683822A1 (en) | 1995-11-29 |
WO1994018342A1 (en) | 1994-08-18 |
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