MX9401112A - Preparacion de conjugados de fotoproteina y metodos para utilizar los mismos. - Google Patents

Preparacion de conjugados de fotoproteina y metodos para utilizar los mismos.

Info

Publication number
MX9401112A
MX9401112A MX9401112A MX9401112A MX9401112A MX 9401112 A MX9401112 A MX 9401112A MX 9401112 A MX9401112 A MX 9401112A MX 9401112 A MX9401112 A MX 9401112A MX 9401112 A MX9401112 A MX 9401112A
Authority
MX
Mexico
Prior art keywords
conjugates
methods
present
preparation
photoprotein
Prior art date
Application number
MX9401112A
Other languages
English (en)
Inventor
Nancy L Stults
Original Assignee
Sealite Sciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sealite Sciences Inc filed Critical Sealite Sciences Inc
Publication of MX9401112A publication Critical patent/MX9401112A/es

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente invención abarca un método para la síntesis de conjugados de fotoproteínas que retienen toda o una porción considerable de la actividad luminiscente de las fotoproteínas no derivatizadas. De conformidad con la presente invención, las fotoproteínas pueden conjugarse con una variedad de enlaces o uniones que incluyen estreptavidina/avidina, glicoproteínas, lectinas, hormonas, antígenos, fármacos, anticuerpos y fragmentos de unión de antígeno, de los mismos, o cualquier otro reactivo selectivamente ligable, por medios de reticulación química. La presente invención también abarca conjugados producidos por este método, y los métodos de uso de esos conjugados.
MX9401112A 1993-02-12 1994-02-11 Preparacion de conjugados de fotoproteina y metodos para utilizar los mismos. MX9401112A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US1711693A 1993-02-12 1993-02-12

Publications (1)

Publication Number Publication Date
MX9401112A true MX9401112A (es) 1994-08-31

Family

ID=21780817

Family Applications (1)

Application Number Title Priority Date Filing Date
MX9401112A MX9401112A (es) 1993-02-12 1994-02-11 Preparacion de conjugados de fotoproteina y metodos para utilizar los mismos.

Country Status (9)

Country Link
US (1) US5486455A (es)
EP (1) EP0683822B1 (es)
JP (1) JPH08506897A (es)
AT (1) ATE217086T1 (es)
AU (1) AU6171894A (es)
DE (1) DE69430523T2 (es)
IL (1) IL108607A (es)
MX (1) MX9401112A (es)
WO (1) WO1994018342A1 (es)

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EP0736770A3 (fr) * 1995-04-05 1997-05-02 Anda Biolog Sa Conjugué immunoréactif, procédé d'obtention de ce conjugué, anticorps dirigés contre ledit conjugué, composition pharmaceutique et dispositif de diagnostic les comprenant
DE19537952A1 (de) 1995-10-12 1997-04-17 Boehringer Mannheim Gmbh Verfahren zum Nachweis eines Analyten
US5876995A (en) 1996-02-06 1999-03-02 Bryan; Bruce Bioluminescent novelty items
US5942407A (en) * 1996-06-25 1999-08-24 Immunomatrix, Inc. Light-emitting immunoassay
US6416960B1 (en) 1996-08-08 2002-07-09 Prolume, Ltd. Detection and visualization of neoplastic tissues and other tissues
ATE290205T1 (de) 1996-12-12 2005-03-15 Prolume Ltd Vorrichtung und verfahren zum nachweis und identifizieren von infektiösen wirkstoffen
DE19652576A1 (de) * 1996-12-17 1998-06-25 Boehringer Mannheim Gmbh Niedermolekulare Photoprotein-Konjugate
US5876935A (en) * 1997-01-08 1999-03-02 Dade Behring Inc. Luminescent specific binding assay
CA2281648A1 (en) 1997-02-13 1998-08-20 Memorial Sloan-Kettering Cancer Center Hybrid molecules for optically detecting changes in cellular microenvironments
JP2001514741A (ja) * 1997-02-18 2001-09-11 オンカー インク． 緑色蛍光蛋白質およびその蛍光変異体による標的の検出
DE19719465A1 (de) * 1997-05-07 1998-11-12 Boehringer Mannheim Gmbh Konservierung von Aequorin-Reagenzien
JP2002507410A (ja) 1998-03-27 2002-03-12 プロルーム・リミテッド ルシフェラーゼ、蛍光タンパク質、ルシフェラーゼおよび蛍光タンパク質をコードする核酸および、診断、高処理スクリーニングおよび新規アイテムにおけるその使用
EP1925320A3 (en) 1998-03-27 2008-09-03 Prolume, Ltd. Luciferases, fluorescent proteins, nucleic acids encoding the luciferases and fluorescent proteins and the use thereof in diagnostics
ATE356355T1 (de) * 1999-06-18 2007-03-15 Cardiogenics Inc Verfahren zur prozessführung chemilumineszenter bindungstests
US7109315B2 (en) * 2000-03-15 2006-09-19 Bruce J. Bryan Renilla reniformis fluorescent proteins, nucleic acids encoding the fluorescent proteins and the use thereof in diagnostics, high throughput screening and novelty items
US7659078B1 (en) * 2002-07-17 2010-02-09 University Of Kentucky Research Foundation Method and kit for determination of prostacyclin in plasma
US20080280310A1 (en) * 2007-05-09 2008-11-13 Louis Panagopoulos Testing for Blood Group Immunological Reaction Without the Use of Anti-Human Globulin

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US4604364A (en) * 1974-01-04 1986-08-05 Kosak Kenneth M Bioluminescent tracer composition and method of use in immunoassays
GB1578275A (en) * 1977-06-14 1980-11-05 Maier C Procedure for the assay of pharmacologically immunologically and biochemically active compounds in biological fluids
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Also Published As

Publication number Publication date
EP0683822A4 (en) 1997-11-05
DE69430523T2 (de) 2002-11-28
JPH08506897A (ja) 1996-07-23
DE69430523D1 (de) 2002-06-06
IL108607A (en) 1998-12-06
IL108607A0 (en) 1994-05-30
EP0683822B1 (en) 2002-05-02
ATE217086T1 (de) 2002-05-15
US5486455A (en) 1996-01-23
AU6171894A (en) 1994-08-29
EP0683822A1 (en) 1995-11-29
WO1994018342A1 (en) 1994-08-18

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