ES2076416T3 - Realizacion de un inmunoensayo segun el principio de la aglutinacion. - Google Patents

Realizacion de un inmunoensayo segun el principio de la aglutinacion.

Info

Publication number
ES2076416T3
ES2076416T3 ES91110369T ES91110369T ES2076416T3 ES 2076416 T3 ES2076416 T3 ES 2076416T3 ES 91110369 T ES91110369 T ES 91110369T ES 91110369 T ES91110369 T ES 91110369T ES 2076416 T3 ES2076416 T3 ES 2076416T3
Authority
ES
Spain
Prior art keywords
agglutination
principle
performance
functional group
assay according
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91110369T
Other languages
English (en)
Inventor
Arno Dr Deger
Francois Dr Guillot
Michael Dr Berger
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Application granted granted Critical
Publication of ES2076416T3 publication Critical patent/ES2076416T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • G01N33/6857—Antibody fragments

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

PARA LA REALIZACION DE UN INMUNOASSAY HOMOGENEO A PARTIR DEL PRINCIPIO DE AGLUTINACION SE ELABORA A) UN CONJUGADO DENTRO DEL CUAL ESTA SUSPENDIDO UN FRAGMENTO -F (AB-) 2 DE UN ANTICUERPO DE LA CLASE G DE INMUNOGLOBULINA EN CONDICIONES REDUCIDAS Y ADEMAS SE PONE EN PRACTICA CON EL COMPONENTE K , QUE O BIEN CONTIENE UN GRUPO FUNCIONAL APROPIADO PARA LA UNION O BIEN SE DERIVA A TRAVES DE UNA IMPLANTACION DE UN GRUPO FUNCIONAL DE MANERA APROPIADA, CON LO QUE SE CONECTA EL COMPONENTE K SOBRE EL GRUPO FUNCIONAL EN EL GRUPO-SH LIBRE DE FRAGMENTO-FAB, QUE SE PRODUCE EN UNA REDUCCION Y B) PARTICULILLAS AGLUTINABLES QUE SOPORTAN UNA SUSTANCIA K FRAGUABLE.
ES91110369T 1990-06-25 1991-06-24 Realizacion de un inmunoensayo segun el principio de la aglutinacion. Expired - Lifetime ES2076416T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE4020204A DE4020204A1 (de) 1990-06-25 1990-06-25 Durchfuehrung eines homogenen immunoassays nach dem agglutinationsprinzip

Publications (1)

Publication Number Publication Date
ES2076416T3 true ES2076416T3 (es) 1995-11-01

Family

ID=6409035

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91110369T Expired - Lifetime ES2076416T3 (es) 1990-06-25 1991-06-24 Realizacion de un inmunoensayo segun el principio de la aglutinacion.

Country Status (5)

Country Link
EP (1) EP0464554B1 (es)
JP (1) JP3000239B2 (es)
AT (1) ATE127232T1 (es)
DE (2) DE4020204A1 (es)
ES (1) ES2076416T3 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1404577A (zh) * 2000-12-11 2003-03-19 株式会社药得论 免疫分析试剂和分析方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6113156A (ja) * 1984-06-28 1986-01-21 Sumitomo Chem Co Ltd ヒトインタ−フエロン−αの定量用試薬及び定量法
US4814433A (en) * 1987-09-16 1989-03-21 Miles Inc. Method for obtaining a papain-free antibody fragment preparation
US4914040A (en) * 1988-03-03 1990-04-03 Boehringer Mannheim Gmbh Reagent and method for determination of a polyvalent substance using an immunoaggregate
DE3822750A1 (de) * 1988-07-05 1990-01-11 Boehringer Mannheim Gmbh Verfahren zur bestimmung einer spezifisch bindefaehigen substanz
DE3829245A1 (de) * 1988-08-29 1990-03-01 Boehringer Mannheim Gmbh Verfahren zur bestimmung einer spezifisch bindefaehigen substanz
JP2665353B2 (ja) * 1988-09-10 1997-10-22 住商ファーマインターナショナル株式会社 双特異性抗体の製造法

Also Published As

Publication number Publication date
EP0464554A3 (en) 1992-10-14
DE59106352D1 (de) 1995-10-05
ATE127232T1 (de) 1995-09-15
EP0464554B1 (de) 1995-08-30
DE4020204A1 (de) 1992-01-02
JPH04232466A (ja) 1992-08-20
JP3000239B2 (ja) 2000-01-17
EP0464554A2 (de) 1992-01-08

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