KR950008679A - 신균주 스트렙토마이세스 라벤도폴리아 DKRS 및 이를 이용한 아클라시노마이신 A,B,Y 및 아글리콘(Aglycone)의 제조방법 - Google Patents

신균주 스트렙토마이세스 라벤도폴리아 DKRS 및 이를 이용한 아클라시노마이신 A,B,Y 및 아글리콘(Aglycone)의 제조방법 Download PDF

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KR950008679A
KR950008679A KR1019930017589A KR930017589A KR950008679A KR 950008679 A KR950008679 A KR 950008679A KR 1019930017589 A KR1019930017589 A KR 1019930017589A KR 930017589 A KR930017589 A KR 930017589A KR 950008679 A KR950008679 A KR 950008679A
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aglycone
kctc
culture
dkrs
medium
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KR1019930017589A
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KR970000591B1 (ko
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조원태
김완섭
김명국
박진규
김학열
이상기
돔라체바
파니치키나
사브로바
노비코바
발토체비치
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권기범
강재헌
동국제약 주식회사
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Priority to KR1019930017589A priority Critical patent/KR970000591B1/ko
Priority to GB9400513A priority patent/GB2281563B/en
Priority to JP6002565A priority patent/JP2718002B2/ja
Priority to ITMI940035A priority patent/IT1273049B/it
Priority to ES09400079A priority patent/ES2078875B1/es
Priority to FR9400527A priority patent/FR2709497B1/fr
Priority to DE4401546A priority patent/DE4401546C2/de
Priority to NL9400096A priority patent/NL9400096A/nl
Priority to TW083100481A priority patent/TW258754B/zh
Priority to CA002113871A priority patent/CA2113871C/en
Priority to CH00304/94A priority patent/CH688151A5/fr
Priority to US08/192,165 priority patent/US5468637A/en
Priority to CN 94105525 priority patent/CN1108694A/zh
Priority to CN94105525A priority patent/CN1104682A/zh
Priority to US08/369,375 priority patent/US5484712A/en
Publication of KR950008679A publication Critical patent/KR950008679A/ko
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    • C12P15/00—Preparation of compounds containing at least three condensed carbocyclic rings
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/06—Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/60—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin
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    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/465—Streptomyces
    • C12R2001/56—Streptomyces lavendulae
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/886—Streptomyces

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Abstract

본 발명은 항암제인 아클라시노마이신들과 그의 아글리콘을 생산하는 스트렙토마이세스속 균주 및 이를 배양하여 아클라시노마이신들과 그의 아글리콘을 생산하는 방법을 제공한다. 본 발명의 균주 스트렙토마이세스 라벤도폴리아(Streptomyces lavendofoliae) DKRs(KCTC 8539P)는 아클라시노마이신 A와 B만을 생산하는 친주인 I2/3A와 달리 A, B, Y 및 아글리콘을 생산하며 그 수율도 높다.
한편 미생물 배양 후 배양액을 염산이나 초산 완충용액으로 처리하면 아클라시노마이신 A가 Y로 또는 Y가 A로 전환되는 것이 관찰되는데, 이는 미생물 자체의 트정 효소의 작용에 의해 일어나는 것으로 생각되며, 이런 작용을 이용하여 아클라시노마이신 A 또는 Y를 선택적으로 생산할 수 있다.

Description

신균주 스트렙토마이세스 라벤도폴리아 DKRS 및 이를 이용한 아클라시노마이신 A, B, Y 및 아글리콘(Aglycone)의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (7)

  1. 아클라시노마이신 A, B, Y 및 아글리콘의 생산기능을 갖는 스트렙토마이세스 라벤도폴리아(Streptolmyces lavendofoliae) DKRs(KCTC 8539P).
  2. 제1항에 있어서, 배양후 배양액을 초산 완충액으로 pH 4.4로 보정하면 아클라시노마이신 A를 Y로 전환시키고, 배양액을 염산으로 pH 4.6으로 보정하면 아클라시노마신 Y를 A로 전환시키는 능력을 가짐을 특징으로 하는 스트렙토마이세스 라벤도폴리아(Streptomyces lavendofoliae) DKRS(KCTC 8539P).
  3. 스트렙토마이세스 라벤도폴리아(Streptomyces lavendofoliae) DKRS(KCTC 8539P)를 배지에 배양하여 아클라시노마이신 A, B, Y 및 아글리콘을 균체내 및 배양액에 축적한 후 균체 및 배양액으로부터 아클라시노마이신 A, B, Y 및 아글리콘을 회수하여 정제함을 특징으로 하는 발효법에 아클라시노마이신 A, B, Y 및 아글리콘의 제조방법.
  4. 제3항에 있어서, 배양은 28-30℃, pH6.8-7.5범위의 호기적 조건에서 4-5일간 실시함을 특징으로 하는 제조방법.
  5. 제3항에 있어서, 톨루엔과 이소프로파놀의 30 : 1-35 : 1부피비의 혼합 용매를 이용하여 아클라시노마이신 A, B, Y 및 아글리콘을 회수 및 정제함을 특징으로 하는 제조방법.
  6. 스트렙토마이세스 라벤도폴리아(Streptomyces lavendofoliae) DKRS(KCTC 8539P)를 배지에 배양하고, 배양액을 초산 완충용액으로 pH 4.4로 보정한 후 균체와 배양액으로부터 아클라시노마이신 Y를 분리함을 특징으로 하는 아클라시노마이신 Y의 제조방법.
  7. 스트렙토마이세스 라벤도폴리아(Streptomyces lavendofoliae) DKRS(KCTC 8539P)를 배지에배양하고, 배양액을 염산으로 pH 4.6으로 보정한 후 균체와 배양액으로부터 아클라시노마이신 A를 분리함을 특징으로 하는 아클라시노마이신 A의 제조방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019930017589A 1993-09-03 1993-09-03 신균주 스트렙토마이세스 라벤도폴리아 DKRS 및 이를 이용한 아클라시노마이신 A, B, Y 및 아글리콘(Aglycone)의 제조방법 KR970000591B1 (ko)

Priority Applications (15)

Application Number Priority Date Filing Date Title
KR1019930017589A KR970000591B1 (ko) 1993-09-03 1993-09-03 신균주 스트렙토마이세스 라벤도폴리아 DKRS 및 이를 이용한 아클라시노마이신 A, B, Y 및 아글리콘(Aglycone)의 제조방법
GB9400513A GB2281563B (en) 1993-09-03 1994-01-12 A strain of streptomyces lavendofoliae dkrs & a method for producing aclacinomycins a,b,y & aglycone by using the same
JP6002565A JP2718002B2 (ja) 1993-09-03 1994-01-14 新菌株ストレプトマイセス・ラベンドフォリアｄｋｒｓならびにこれを用いたアクラシノマイシンａ，ｂ，ｙおよびアグリコンの製造方法
ITMI940035A IT1273049B (it) 1993-09-03 1994-01-14 Ceppo di streptomyces lavendofoliae dkrs e metodo per la sua utilizzazione nella produzione delle aclacinomicine a,b,y e dell'aglicone
ES09400079A ES2078875B1 (es) 1993-09-03 1994-01-17 Procedimiento para la produccion de aclacinomicinas a,b, y, y sus agluconas mediante el cultivo de la cepa streptomyces lavendofoliae dkrs.
FR9400527A FR2709497B1 (fr) 1993-09-03 1994-01-19 Souche de Streptomyces lavendofoliae DKRS et procédé de production des aclacinomycines A, B, Y et d'aglycone en utilisant ceux-ci.
DE4401546A DE4401546C2 (de) 1993-09-03 1994-01-20 Streptomyces lavendofoliae DRKS Stamm, Verfahren zur Gewinnung der Aclacinomycine A, B, Y und von Aglycon durch Anwendung dieses Verfahrens
NL9400096A NL9400096A (nl) 1993-09-03 1994-01-20 Nieuwe Streptomyces-stam en werkwijze voor het bereiden van antitumormiddelen daarmee.
TW083100481A TW258754B (ko) 1993-09-03 1994-01-20
CA002113871A CA2113871C (en) 1993-09-03 1994-01-20 A strain of streptomyces lavendofoliae dkrs and a method for producing aclacinomycins a,b,y and aglycones using the same
CH00304/94A CH688151A5 (fr) 1993-09-03 1994-02-02 Souche de streptomyces lavendofoliae DKRS et procédé de production des aclacinomycines A, B, Y et d'aglycone en utilisant ceux-ci.
US08/192,165 US5468637A (en) 1993-09-03 1994-02-04 S. lavendofoliae DKRS cell culture producing anti-tumor aclacinomycins A, B, Y and aglycones
CN 94105525 CN1108694A (zh) 1993-09-03 1994-05-17 一种淡紫灰叶链霉菌dkrs菌株及用它制备阿克拉希霉素a，b，y和糖苷配基的方法
CN94105525A CN1104682A (zh) 1993-09-03 1994-05-17 一种淡紫灰叶链霉菌dkrs菌株及用它制备阿克拉希霉素a，b，y和糖苷配基的方法
US08/369,375 US5484712A (en) 1993-09-03 1995-01-06 Method for producing aclacinomycins A, B, Y using Strepomyces lavendofoliae DKRS

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019930017589A KR970000591B1 (ko) 1993-09-03 1993-09-03 신균주 스트렙토마이세스 라벤도폴리아 DKRS 및 이를 이용한 아클라시노마이신 A, B, Y 및 아글리콘(Aglycone)의 제조방법

Publications (2)

Publication Number Publication Date
KR950008679A true KR950008679A (ko) 1995-04-19
KR970000591B1 KR970000591B1 (ko) 1997-01-14

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KR1019930017589A KR970000591B1 (ko) 1993-09-03 1993-09-03 신균주 스트렙토마이세스 라벤도폴리아 DKRS 및 이를 이용한 아클라시노마이신 A, B, Y 및 아글리콘(Aglycone)의 제조방법

Country Status (13)

Country Link
US (2) US5468637A (ko)
JP (1) JP2718002B2 (ko)
KR (1) KR970000591B1 (ko)
CN (2) CN1104682A (ko)
CA (1) CA2113871C (ko)
CH (1) CH688151A5 (ko)
DE (1) DE4401546C2 (ko)
ES (1) ES2078875B1 (ko)
FR (1) FR2709497B1 (ko)
GB (1) GB2281563B (ko)
IT (1) IT1273049B (ko)
NL (1) NL9400096A (ko)
TW (1) TW258754B (ko)

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5134915B2 (ko) * 1974-07-27 1976-09-29
US4071411A (en) * 1974-07-27 1978-01-31 Zaidan Hojin Biseibutsu Kagaku Kenkyu Kai Antitumor antibiotics aclacinomycins A and B [RD-9187A]
US4204038A (en) * 1976-04-07 1980-05-20 Zaidan Hojin Biseibutsu Kagaku Kenkyu Kai Process for preparing antibiotics MA 144-M1 and MA 144-M2
NL176004C (nl) * 1976-10-05 1985-02-01 Microbial Chem Res Found Werkwijze ter bereiding van geneesmiddelen met antitumorwerking op basis van anthracycline glycosiden en werkwijze ter bereiding van daartoe dienende anthracycline glycosiden.
US4209588A (en) * 1976-10-05 1980-06-24 Zaidan Hojin Biseibutsu Kagaku Kenkyu Kai Process for producing new antitumor anthracycline antibiotics
JPS5648893A (en) * 1979-09-07 1981-05-02 Sanraku Inc Preparation of anthracycline glycoside
DE3164574D1 (en) * 1980-10-27 1984-08-09 Hoffmann La Roche Process for aclacinomycins and microorganism used therein
DE3168154D1 (en) * 1980-10-27 1985-02-21 Hoffmann La Roche Process for anthracycline glycosides
US4405713A (en) * 1981-12-28 1983-09-20 Hoffmann-La Roche Inc. Process for the preparation of optically active anthracycline glycosides A and B
US4471052A (en) * 1982-01-18 1984-09-11 Adria Laboratories, Inc. Biosynthesis of simplified anthracyclines
SU1069433A1 (ru) * 1982-06-29 1984-11-23 Всесоюзный научно-исследовательский институт антибиотиков Штамм @ @ 12/3а-продуцент аклациномицинов @ и @

Also Published As

Publication number Publication date
CH688151A5 (fr) 1997-05-30
ES2078875B1 (es) 1996-08-16
US5484712A (en) 1996-01-16
US5468637A (en) 1995-11-21
ITMI940035A1 (it) 1995-07-14
DE4401546C2 (de) 1997-09-25
TW258754B (ko) 1995-10-01
GB9400513D0 (en) 1994-03-09
CA2113871C (en) 2000-01-18
CN1104682A (zh) 1995-07-05
GB2281563B (en) 1998-01-28
JPH0775575A (ja) 1995-03-20
NL9400096A (nl) 1995-04-03
DE4401546A1 (de) 1995-03-30
IT1273049B (it) 1997-07-01
ITMI940035A0 (it) 1994-01-14
KR970000591B1 (ko) 1997-01-14
GB2281563A (en) 1995-03-08
CN1108694A (zh) 1995-07-05
JP2718002B2 (ja) 1998-02-25
CA2113871A1 (en) 1995-03-04
FR2709497A1 (fr) 1995-03-10
ES2078875A1 (es) 1995-12-16
FR2709497B1 (fr) 1996-11-15

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