KR950006460A - 피검물질의 활성측정법 - Google Patents

피검물질의 활성측정법 Download PDF

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KR950006460A
KR950006460A KR1019940017772A KR19940017772A KR950006460A KR 950006460 A KR950006460 A KR 950006460A KR 1019940017772 A KR1019940017772 A KR 1019940017772A KR 19940017772 A KR19940017772 A KR 19940017772A KR 950006460 A KR950006460 A KR 950006460A
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activity
measuring
test substance
plasma kallikrein
substance
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KR1019940017772A
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KR100270160B1 (ko
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카쭈미 니시카와
히토시 카와쿠보
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코니시 진우에몬
닙뽄조오키세이야쿠 가부시키가이샤
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/56—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving blood clotting factors, e.g. involving thrombin, thromboplastin, fibrinogen
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2337/00—N-linked chromogens for determinations of peptidases and proteinases
    • C12Q2337/10—Anilides
    • C12Q2337/12—Para-Nitroanilides p-NA
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
    • G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
    • G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
    • G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
    • G01N2333/96433—Serine endopeptidases (3.4.21)
    • G01N2333/96441—Serine endopeptidases (3.4.21) with definite EC number
    • G01N2333/96455—Kallikrein (3.4.21.34; 3.4.21.35)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
    • G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
    • G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
    • G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
    • G01N2333/96433—Serine endopeptidases (3.4.21)
    • G01N2333/96441—Serine endopeptidases (3.4.21) with definite EC number
    • G01N2333/96458—Factor XII (3.4.21.38)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Neurosurgery (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

재구성한 혈장칼리크레인·키닌계를 이용한 피검물질의 활성측정법을 제공한다.
재구성한 혈장칼리크레인·키닌계에 있어서 피검물질의 존재하, 혈액응고제 XⅡ인자의 활성화를 초발반응으로 하는 일련의 효소반응을 개시시킨 후, 이 반응을 정지시키고 상기한 반응에서 생성된 생리활성물질을 정량한다.
본 발명의 활성측정법에 있어서는 복잡한 그 외 인자를 실질적으로 제거한 반응계에 의해서, 생성되는 생리활성물질의 생성에 대한 피검물질의 순수한 활성(촉진능 또는 저해능)을 간단신속하고 또한 정확하게 측정할 수 있다. 따라서 본 발명의 활성측정법은 혈장칼리크레인·키닌계, 혈액응고계, 선용계 등의 생체기능의 조정에 유용한 작용을 알고 있고, 또는 이와 같은 약제를 개발하는데 매우 유효한 수단이 된다.

Description

피검물질의 활성측정법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 본 발명의 실시예1 및 실시예2에 있어서, 각종 인큐베이션시간에 있어서의 흡광도의 변화를 나타낸다.
제2도는 본 발명의 활성측정법을 사용하여 각종 피검약의 혈장칼리크레인생성에 대한 저해활성을 측정한 결과를 나타낸다.
제3도는 혈장칼리크레인·키닌계의 효소반응기구를 설명한 것이다.

Claims (7)

  1. 재구성한 혈장칼리크레인·키닌계에 있어서 피검물질의 존재하, 혈액응고제 XⅡ인자의 활성화를 초발반응으로 하는 일련의 효소반응을 개시시킨 후, 이 반응을 정지시키고 상기한 반응에서 생성된 생리활성물질을 정량하는 것을 특징으로 하는 피검물질의 활성측정법.
  2. 제1항에 있어서, 생리활성물질의 생성량이 포화에 달하기 전에 그 생리활성물질의 생성을 정지시키는 피검물질의 활성측정법.
  3. 제2항에 있어서, 생리활성물질이 활성형 혈액응고제 XⅡ인자인 피검물질의 활성측정법.
  4. 제2항에 있어서, 생리활성물질이 혈장칼리크레인인 피검물질의 활성측정법.
  5. 제2항에 있어서, 생리활성물질이 브랜디키닌인 피검물질의 활성측정법.
  6. 제1항 내지 제4항중 어느 한 항에 있어서, 혈액응고제 XⅡ인자, 혈장프레칼리크레인으로 재구성한 계인 피검물질의 활성측정법.
  7. 제1항 내지 제4항중 어느 한 항에 있어서, 혈액응고제 XⅡ인자, 혈장프레칼리크레인, 고분자키니노이겐으로 재구성한 계인 피검물질의 활성측정법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019940017772A 1993-08-06 1994-07-22 피검물질의 활성 측정법 KR100270160B1 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP21517893A JP3213832B2 (ja) 1993-08-06 1993-08-06 被検物質の活性測定法
JP93-215178 1993-08-06

Publications (2)

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KR950006460A true KR950006460A (ko) 1995-03-21
KR100270160B1 KR100270160B1 (ko) 2000-10-16

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US (1) US5648228A (ko)
EP (1) EP0637632B1 (ko)
JP (1) JP3213832B2 (ko)
KR (1) KR100270160B1 (ko)
CN (1) CN1080762C (ko)
AT (1) ATE194659T1 (ko)
DE (1) DE69425197T2 (ko)
ES (1) ES2149833T3 (ko)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH1084995A (ja) * 1996-09-12 1998-04-07 Nippon Zoki Pharmaceut Co Ltd 血液凝固第ｘｉｉ因子活性化法
WO2001063299A1 (en) * 2000-02-23 2001-08-30 Besst-Test Aps METHOD FOR CORRELATING BLOOD COAGULATION ACTIVITY WITH MARKERS IN BODY FLUIDS, e.g. URINE
US6632678B2 (en) 2001-01-03 2003-10-14 Sienco, Inc. Method for performing activated clotting time test with reduced sensitivity to the presence of aprotinin and for assessing aprotinin sensitivity
US6913900B2 (en) 2001-08-29 2005-07-05 Nippon Zoki Pharmaceutical Co., Ltd. Plasma prekallikrein activation and kallikrein production assay

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB697351A (en) * 1951-01-16 1953-09-23 Masuichi Takino Improved process for the manufacture of nerve sedatives
JPS53101515A (en) * 1977-02-17 1978-09-05 Nippon Zoki Pharmaceutical Co Medicine having anodyne * sedative and antiallergic activity and production thereof
US4335203A (en) * 1980-07-10 1982-06-15 The Regents Of The University Of California Method for identifying potential contrast media reactors
JPS5777697A (en) * 1980-11-04 1982-05-15 Nippon Zoki Pharmaceut Co Ltd Physiologically active substance nsq
JPS5835117A (ja) * 1981-08-25 1983-03-01 Nippon Zoki Pharmaceut Co Ltd 新規生理活性物質ｎｓｈ
ATE17262T1 (de) * 1981-11-02 1986-01-15 Pentapharm Ag Verfahren zur quantitativen bestimmung von blutgerinnungsfaktor xii in humanplasma.
DE3504405A1 (de) * 1985-02-08 1986-08-14 Boehringer Mannheim Gmbh, 6800 Mannheim Bestimmung von prokallikrein
JPS63185398A (ja) * 1986-09-10 1988-07-30 Nippon Zoki Pharmaceut Co Ltd 生理活性物質測定法
US4882272A (en) * 1986-10-01 1989-11-21 Temple University - Of The Commonwealth System Of Higher Education High molecular weight kininogen assay
JP2651674B2 (ja) * 1987-07-23 1997-09-10 日本臓器製薬 株式会社 新規生理活性物質及びその製造方法
US4985254A (en) * 1987-11-06 1991-01-15 Nippon Zoki Pharmaceutical Co., Ltd. Method of treating ischemic diseases
DE68909100T2 (de) * 1988-04-30 1994-01-13 Nippon Zoki Pharmaceutical Co Physiologisch wirkende Substanzen, Verfahren zu ihrer Herstellung und pharmazeutische Zusammensetzungen davon.
JP2539665B2 (ja) * 1988-06-20 1996-10-02 日本臓器製薬株式会社 神経疾患治療剤
CA2082643A1 (en) * 1990-05-10 1991-11-11 Jan H. Nuijens Inhibitors of factor xii activation and applications thereof

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Publication number Publication date
ES2149833T3 (es) 2000-11-16
EP0637632A3 (en) 1998-01-07
US5648228A (en) 1997-07-15
DE69425197D1 (de) 2000-08-17
DE69425197T2 (de) 2001-03-15
KR100270160B1 (ko) 2000-10-16
ATE194659T1 (de) 2000-07-15
EP0637632A2 (en) 1995-02-08
JP3213832B2 (ja) 2001-10-02
CN1080762C (zh) 2002-03-13
EP0637632B1 (en) 2000-07-12
CN1110408A (zh) 1995-10-18
JPH0751098A (ja) 1995-02-28

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