KR940005659A - 5-뉴클레오티드의 제조방법 - Google Patents

5-뉴클레오티드의 제조방법 Download PDF

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KR940005659A
KR940005659A KR1019930012540A KR930012540A KR940005659A KR 940005659 A KR940005659 A KR 940005659A KR 1019930012540 A KR1019930012540 A KR 1019930012540A KR 930012540 A KR930012540 A KR 930012540A KR 940005659 A KR940005659 A KR 940005659A
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nucleoside
suspension
organic solvent
maintained
nucleotides
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KR1019930012540A
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KR100270246B1 (ko
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아끼라 하제
히로유기 하따노
도모미 이께모또
요시후미 기따모또
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다께다 구니오
다께다야꾸힝고오교가부시끼가이샤
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C07H19/207—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids the phosphoric or polyphosphoric acids being esterified by a further hydroxylic compound, e.g. flavine adenine dinucleotide or nicotinamide-adenine dinucleotide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

온도로 유기 용매중의 뉴클레오시드 현탁액을 20℃ 이상의 온도로 유지하고, 이어서 얻어진 현탁액을 뉴클레오시드의 부인산화하는 것을 특징으로 하는 5'-뉴클레오티드의 제조방법이 개시된다.
본 발명에 따르면, 5'-뉴클레오티드는 단축된 반응 시간으로, 고순도 및 고수율으로 뉴클레오시드로 부터 5'-뉴클레오티드를 제조할 수 있으며, 뉴클레오티드 정제과정에서 불순물의 제거도 용이하게 수행된다.

Description

5'-뉴클레오티드의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (13)

  1. 약 20℃ 이상의 온도로 유기 용매에 뉴클레오티드 현탁액을 유지하고, 이어서 얻어진 현탁액을 뉴클레오시드의 부인산화(phosphorylation) 하는 것을 특징으로 하는 5'-뉴클레오티드의 제조방법.
  2. 제1항에 있어서, 유기 용매에 뉴클레오시드 현탁액을 약30~약80℃로 유지하는 것을 특징으로 하는 방법.
  3. 제1항에 있어서, 유기 용매중의 뉴클레오시드 현탁액을 20℃ 이상의 온도로 약10~약120분간 유지하는 것을 특징으로 하는 방법.
  4. 제1항에 있어서, 부인산화는 약-30~약10℃에서 수행되는 것을 특징으로 하는 방법.
  5. 제1항에 있어서, 유기 용매는 트리-저급(C1~6) 알킬 포스페이트인 것을 특징으로 하는 방법.
  6. 제5항에 있어서, 트리-저급(C1~6) 알킬 포스페이트는 트리에틸포스페이트인 것을 특징으로 하는 방법.
  7. 제1항에 있어서, 부인산화는 옥시산화인을 사용하여 수행되는 것을 특징으로 하는 방법.
  8. 제7항에 있어서, 옥시할로겐화인은 옥시염화인인 것을 특징으로 하는 방법.
  9. 제7항에 있어서, 옥시할로겐화인은 뉴클레오시드당 약1~약5몰의 양으로 사용하는 것을 특징으로 하는 방법.
  10. 제1항에 있어서, 뉴클레오시드는 이노신인 것을 특징으로 하는 방법.
  11. 제1항에 있어서, 뉴클레오시드는 구아노신인 것을 특징으로 하는 방법.
  12. 제1항에 있어서, 뉴클레오시드는 이노신 및 구아신의 혼합 결정인 것을 특징으로 하는 방법.
  13. 제1항에 있어서, 뉴클레오시드는 시티딘인 것을 특징으로 하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019930012540A 1992-07-08 1993-07-05 5'-뉴클레오티드의 제조방법 KR100270246B1 (ko)

Applications Claiming Priority (2)

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JP18114592 1992-07-08
JP92-181145 1992-07-08

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KR940005659A true KR940005659A (ko) 1994-03-22
KR100270246B1 KR100270246B1 (ko) 2000-10-16

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US (1) US5623069A (ko)
KR (1) KR100270246B1 (ko)
CN (1) CN1033272C (ko)
CA (1) CA2100027C (ko)
ES (1) ES2055670B1 (ko)
IT (1) IT1264061B (ko)
TW (1) TW279165B (ko)

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Publication number Priority date Publication date Assignee Title
CN101665525B (zh) * 2009-09-10 2013-05-01 广东肇庆星湖生物科技股份有限公司 一种核苷酸的合成方法
CN101891772B (zh) * 2010-07-16 2012-11-28 广东肇庆星湖生物科技股份有限公司 一种5’-核苷酸二钠的制备方法
CN101914125A (zh) * 2010-08-12 2010-12-15 广东肇庆星湖生物科技股份有限公司 一种5’-鸟苷磷酸化反应液的萃取工艺
CN104151383A (zh) * 2014-07-15 2014-11-19 南通香地生物有限公司 尿苷酸二钠盐的生产方法
CN114315934A (zh) * 2021-12-22 2022-04-12 成都市海通药业有限公司 一种胞磷胆碱重要中间体胞苷酸的合成和精制方法

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1645896C3 (de) * 1965-03-17 1975-01-02 Ajinomoto Co., Inc., Tokio Verfahren zur Herstellung von 5'-Ribonucleotiden nd S'-Desoxyribonucleotiden
DE1620568A1 (de) * 1965-10-04 1970-08-27 Takeda Chemical Industries Ltd Verfahren zur Herstellung von Nucleotiden
US3433783A (en) * 1967-07-03 1969-03-18 Takeda Chemical Industries Ltd Method for the production of ribonucleoside-5'-phosphate
JPS5980694A (ja) * 1982-10-30 1984-05-10 Seikagaku Kogyo Co Ltd リン酸化合物の製造方法
JPS59163397A (ja) * 1983-03-09 1984-09-14 Seikagaku Kogyo Co Ltd リン酸化合物の製造方法
JPS59167599A (ja) * 1983-03-11 1984-09-21 Takeda Chem Ind Ltd イノシンおよびグアノシンの化学的リン酸化法
JP2671446B2 (ja) * 1988-10-25 1997-10-29 味の素株式会社 イノシン及びグアノシンの混合リン酸化方法

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TW279165B (ko) 1996-06-21
CA2100027A1 (en) 1994-01-09
ES2055670A1 (es) 1994-08-16
CN1086219A (zh) 1994-05-04
ITTO930501A0 (it) 1993-07-07
IT1264061B (it) 1996-09-09
ITTO930501A1 (it) 1995-01-07
CA2100027C (en) 2003-10-07
ES2055670B1 (es) 1995-02-16
KR100270246B1 (ko) 2000-10-16
CN1033272C (zh) 1996-11-13
US5623069A (en) 1997-04-22

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