KR920700292A - 아세틸화된 이종 폴리펩티드 - Google Patents
아세틸화된 이종 폴리펩티드Info
- Publication number
- KR920700292A KR920700292A KR1019900702445A KR900702445A KR920700292A KR 920700292 A KR920700292 A KR 920700292A KR 1019900702445 A KR1019900702445 A KR 1019900702445A KR 900702445 A KR900702445 A KR 900702445A KR 920700292 A KR920700292 A KR 920700292A
- Authority
- KR
- South Korea
- Prior art keywords
- somatotropin
- lysine
- substituted
- animal
- arginine
- Prior art date
Links
- 229920001184 polypeptide Polymers 0.000 title claims 4
- 102000004196 processed proteins & peptides Human genes 0.000 title claims 4
- 108090000765 processed proteins & peptides Proteins 0.000 title claims 4
- 102000018997 Growth Hormone Human genes 0.000 claims abstract 11
- 108010051696 Growth Hormone Proteins 0.000 claims abstract 11
- 241001465754 Metazoa Species 0.000 claims abstract 6
- 239000004475 Arginine Substances 0.000 claims abstract 5
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims abstract 5
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 claims abstract 4
- 125000003588 lysine group Chemical group [H]N([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 claims abstract 4
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 claims abstract 2
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 claims abstract 2
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims 5
- 239000004472 Lysine Substances 0.000 claims 5
- 125000000637 arginyl group Chemical group N[C@@H](CCCNC(N)=N)C(=O)* 0.000 claims 3
- 244000005700 microbiome Species 0.000 claims 3
- 125000000404 glutamine group Chemical group N[C@@H](CCC(N)=O)C(=O)* 0.000 claims 2
- 238000000034 method Methods 0.000 claims 2
- 241000894006 Bacteria Species 0.000 claims 1
- 241000283690 Bos taurus Species 0.000 claims 1
- 102000053602 DNA Human genes 0.000 claims 1
- 241000124008 Mammalia Species 0.000 claims 1
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 1
- 108020004511 Recombinant DNA Proteins 0.000 claims 1
- 230000000397 acetylating effect Effects 0.000 claims 1
- 125000000539 amino acid group Chemical group 0.000 claims 1
- 150000001413 amino acids Chemical class 0.000 claims 1
- 238000000855 fermentation Methods 0.000 claims 1
- 230000004151 fermentation Effects 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/1072—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups
- C07K1/1077—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups by covalent attachment of residues other than amino acids or peptide residues, e.g. sugars, polyols, fatty acids
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Endocrinology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Zoology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
내용 없음
Description
내용없음
Claims (11)
- 낮은 농도의 아미노산을 갖는 발효배지상에서 이종 폴리펩티드를 암호화하는 DNA서열로 형질전환된 숙주 미생물을 생융시킴을 포함하는, 상기 미생물에 의해 발현된 이종 폴리펩티드의 아세틸화 방법.
- 제1항에 있어서, 상기 미생물이 상기 이종 폴리펩티드를 암호화하는 재조합 DNA분자로 형질전환된 세균인 방법.
- 157, 167, 171, 및 180번 위치의 라이신에 상응하는 최소한 하나의 아미노산 잔기를 아세틸화시킨 bst.
- 챠트 1에 예시된 천연 소마토트로핀의 157, 167, 171 또는 180번 잔기에 상응하는 최소한 하나의 라이신 잔기가 알기닌으로 치환된 동물 소마토트로핀.
- 제4항에 있어서, 상기 동물이 포유동물인 동물 소마토트로핀.
- 제5항에 있어서, 상기 포유동물인 동물이 소인 포유동물 소마토트로핀.
- 제5항에 있어서, 상기 포유동물인 동물이 돼지인 포유동물 소마토트로핀.
- 제6항에 있어서, 모든 라이신 잔기가 알기닌으로 치환된 소마토트로핀.
- 제6항에 있어서, 157, 167 및 171번 라이신이 알기닌으로 치환되고, 180번 라이신이 글루타민으로 치환된 소마토트로핀.
- 제6항에 있어서, 157, 167, 171 및 180번 라이신이 알기닌 또는 글루타민중 어느 하나에 의해 치환된 소마토트로핀.
- 제6항에 있어서, 157, 167, 171 및 180번 라이신이 모두 글루타민으로 치환된 소마토트로핀.※ 참고사항 : 최초출원 내용에 의하여 공개되는 것임.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US32390189A | 1989-03-15 | 1989-03-15 | |
| US07/323901 | 1989-03-15 | ||
| PCT/US1990/000326 WO1990010706A1 (en) | 1989-03-15 | 1990-01-23 | Acetylated heterologous polypeptides |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR920700292A true KR920700292A (ko) | 1992-02-19 |
Family
ID=23261207
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1019900702445A KR920700292A (ko) | 1989-03-15 | 1990-01-23 | 아세틸화된 이종 폴리펩티드 |
Country Status (11)
| Country | Link |
|---|---|
| EP (2) | EP0719792A2 (ko) |
| JP (1) | JPH04504060A (ko) |
| KR (1) | KR920700292A (ko) |
| AT (1) | ATE142270T1 (ko) |
| AU (1) | AU629929B2 (ko) |
| CA (1) | CA2045173A1 (ko) |
| DE (1) | DE69028396T2 (ko) |
| DK (1) | DK0463115T3 (ko) |
| ES (1) | ES2091241T3 (ko) |
| HU (1) | HUT58796A (ko) |
| WO (1) | WO1990010706A1 (ko) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6140071A (en) * | 1994-01-27 | 2000-10-31 | Somatogen, Inc. | Proteins with mutations to decrease N-terminal methylation |
| CN100402649C (zh) * | 2000-05-10 | 2008-07-16 | 阿斯比奥制药株式会社 | 在基因重组多肽的生产中抑制副产物生成的方法 |
-
1990
- 1990-01-23 DE DE69028396T patent/DE69028396T2/de not_active Expired - Fee Related
- 1990-01-23 JP JP2505682A patent/JPH04504060A/ja active Pending
- 1990-01-23 EP EP96101997A patent/EP0719792A2/en not_active Withdrawn
- 1990-01-23 AT AT90906035T patent/ATE142270T1/de not_active IP Right Cessation
- 1990-01-23 AU AU54060/90A patent/AU629929B2/en not_active Ceased
- 1990-01-23 HU HU903721A patent/HUT58796A/hu unknown
- 1990-01-23 ES ES90906035T patent/ES2091241T3/es not_active Expired - Lifetime
- 1990-01-23 KR KR1019900702445A patent/KR920700292A/ko not_active Application Discontinuation
- 1990-01-23 CA CA002045173A patent/CA2045173A1/en not_active Abandoned
- 1990-01-23 DK DK90906035.2T patent/DK0463115T3/da active
- 1990-01-23 EP EP90906035A patent/EP0463115B1/en not_active Expired - Lifetime
- 1990-01-23 WO PCT/US1990/000326 patent/WO1990010706A1/en active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| ATE142270T1 (de) | 1996-09-15 |
| EP0719792A2 (en) | 1996-07-03 |
| JPH04504060A (ja) | 1992-07-23 |
| DK0463115T3 (ko) | 1997-02-17 |
| HUT58796A (en) | 1992-03-30 |
| EP0463115B1 (en) | 1996-09-04 |
| AU5406090A (en) | 1990-10-09 |
| DE69028396D1 (de) | 1996-10-10 |
| EP0719792A3 (ko) | 1996-07-24 |
| HU903721D0 (en) | 1991-12-30 |
| CA2045173A1 (en) | 1990-09-16 |
| ES2091241T3 (es) | 1996-11-01 |
| EP0463115A1 (en) | 1992-01-02 |
| DE69028396T2 (de) | 1997-02-13 |
| WO1990010706A1 (en) | 1990-09-20 |
| AU629929B2 (en) | 1992-10-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A201 | Request for examination | ||
| E902 | Notification of reason for refusal | ||
| E601 | Decision to refuse application |