KR920000921A - 증대된 전기영동 침전에 의한 생체분자의 고정법 - Google Patents

증대된 전기영동 침전에 의한 생체분자의 고정법 Download PDF

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Publication number
KR920000921A
KR920000921A KR1019910010790A KR910010790A KR920000921A KR 920000921 A KR920000921 A KR 920000921A KR 1019910010790 A KR1019910010790 A KR 1019910010790A KR 910010790 A KR910010790 A KR 910010790A KR 920000921 A KR920000921 A KR 920000921A
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South Korea
Prior art keywords
biomolecule
electrode
sensing electrode
electrodeposited
solution
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KR1019910010790A
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English (en)
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윌리스 존슨 커크
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리로이 휘테커
일라이 릴리 앤드 캄파니
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Publication of KR920000921A publication Critical patent/KR920000921A/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/002—Electrode membranes
    • C12Q1/003—Functionalisation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/817—Enzyme or microbe electrode

Abstract

내용 없음

Description

증대된 전기영동 침전에 의한 생체분자의 고정법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 목적하는 생체분자를 함유하는 수성 유체중에 함침된 생체분자감지 전극상에 생체분자를 전착시키기 위한 장치를 예시하는 도이다.

Claims (3)

  1. 생체분자의 변성을 방지하기에 충분한 범위내에서, 전착시키려는 생체분자의 모든 종이 그 각각의 등전점에 의해 동일한 순 전하 극성을 갖도록 하는 값으로 완충시킨, 전착시키려는 적어도 한종의 생체분자 용액을 제조하고, 상기 생체분자 감지 전극과 반대 전극을 상기 용액에 함침시킨후, 1V미만의 가변 전위(이 전위는 전착시키려는 생체분자를 상기 생체분자 감지 전극쪽으로 이동시키고 그 전극상에 축적시키기에 충분한 실질적으로 일정한 전류를 상기 전극사이에 제공하도록 선택된 방식으로 변화 시킨다)를 상기 생체분자 감지 전극과 반대 전극사이에 가하는 단계를 포함하는, 생체분자 성분을 갖는 생체분자 감지 전극의 제조방법.
  2. 단백질의 변성을 방지하기에 충분한 범위내로 완충시킨, 전착시키려는 적어도 한 종의 단백질 용액을 제조하고, 상기 생체분자 감지 전극 및 반대 전극을 상기 용액중에 합침시킨후, 1V미만의 가변 전위(이 전위는 전착시키려는 생체분자를 상기 생체분자 감지 전극쪽으로 이동시키고 그 전극상에 축적시키기에 충분한 실질적으로 일정한 전류를 상기 전극사이에 제공하도록 선택된 방식으로 시간에 따라 변화시킨다)를 상기 생체분자 감지 전극과 반대 전극 사이에 가하고, 상기 생체분자 감지 전극상에 전착된 상기 축적물을 가교결합제로 가교결합시켜 상기 생체분자 감지 전극상에 수-불용성 괴를 형성시키는 단계를 포함하는, 단백질성 성분을 갖는 생체분자 감지 전극의 제조방법.
  3. 생체분자 감지 전극상에 전착시키려는 글루코즈 옥시다제의 용액을 제조하고, 상기 생체분자 감지 전극 및 반대 전극을 상기 용액중에 함침시킨 후, 1V미만의 가변 전위(이 전위는 전착시키려는 글루코즈 옥시다제를 생체분자 감지 전극쪽으로 이동시키고 그 전극상에 10㎛이상의 필름을 축적시키기에 충분한 실질적으로 일정한 전류를 상기 전극사이에 제공하도록 선택된 방식으로 시간에 따라 변화시킨다)를 상기 생체분자 감지 전극과 반대 전극 사이에 가하는 단계를 포함하는 효소 생체분자 감지기의 제조방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개되는 것임.
KR1019910010790A 1990-06-29 1991-06-27 증대된 전기영동 침전에 의한 생체분자의 고정법 KR920000921A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/546,101 US5166063A (en) 1990-06-29 1990-06-29 Immobolization of biomolecules by enhanced electrophoretic precipitation
US07/546,101 1990-06-29

Publications (1)

Publication Number Publication Date
KR920000921A true KR920000921A (ko) 1992-01-29

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US (1) US5166063A (ko)
EP (1) EP0463859B1 (ko)
JP (1) JP3100422B2 (ko)
KR (1) KR920000921A (ko)
CN (1) CN1057672A (ko)
AR (1) AR243937A1 (ko)
AT (1) ATE122724T1 (ko)
AU (1) AU649686B2 (ko)
CA (1) CA2045200C (ko)
CZ (1) CZ280025B6 (ko)
DE (1) DE69109754T2 (ko)
DK (1) DK0463859T3 (ko)
ES (1) ES2072544T3 (ko)
HU (1) HUT60544A (ko)
IE (1) IE67131B1 (ko)
IL (1) IL98589A (ko)
MX (1) MX173121B (ko)
MY (1) MY106367A (ko)
NO (1) NO912543L (ko)
NZ (1) NZ238646A (ko)
PT (1) PT98075B (ko)
RU (1) RU2068181C1 (ko)
YU (1) YU114891A (ko)
ZA (1) ZA914807B (ko)

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JP3100422B2 (ja) 2000-10-16
PT98075B (pt) 1998-11-30
PT98075A (pt) 1993-06-30
CS192791A3 (en) 1992-02-19
ES2072544T3 (es) 1995-07-16
HUT60544A (en) 1992-09-28
IE912284A1 (en) 1992-01-01
IL98589A (en) 1997-06-10
IE67131B1 (en) 1996-03-06
MY106367A (en) 1995-05-30
AU649686B2 (en) 1994-06-02
NO912543D0 (no) 1991-06-27
IL98589A0 (en) 1992-07-15
YU114891A (sh) 1994-01-20
DK0463859T3 (da) 1995-07-10
JPH04331362A (ja) 1992-11-19
AU7939191A (en) 1992-01-02
EP0463859B1 (en) 1995-05-17
MX173121B (es) 1994-02-01
CA2045200A1 (en) 1991-12-30
CA2045200C (en) 2000-05-30
RU2068181C1 (ru) 1996-10-20
DE69109754D1 (de) 1995-06-22
NO912543L (no) 1991-12-30
US5166063A (en) 1992-11-24
ZA914807B (en) 1993-02-24
CZ280025B6 (cs) 1995-09-13
EP0463859A2 (en) 1992-01-02
AR243937A1 (es) 1993-09-30
ATE122724T1 (de) 1995-06-15
EP0463859A3 (en) 1993-05-12
CN1057672A (zh) 1992-01-08
DE69109754T2 (de) 1995-11-02
NZ238646A (en) 1994-04-27
HU912199D0 (en) 1991-12-30

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