KR910018552A - 스트렙토마이세테스내에서의 조절된 유전자 발현 - Google Patents

스트렙토마이세테스내에서의 조절된 유전자 발현 Download PDF

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KR910018552A
KR910018552A KR1019910005820A KR910005820A KR910018552A KR 910018552 A KR910018552 A KR 910018552A KR 1019910005820 A KR1019910005820 A KR 1019910005820A KR 910005820 A KR910005820 A KR 910005820A KR 910018552 A KR910018552 A KR 910018552A
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South Korea
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kor
temperature
streptomycetes
gene expression
gene
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KR1019910005820A
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KR0168670B1 (ko
Inventor
볼레벤 볼프강
무트 권터
필러 알프레드
요한네스 리이스 권터
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테르가우, 라피스
훽스트 아크티엔게젤샤프트
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Publication of KR910018552A publication Critical patent/KR910018552A/ko
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Publication of KR0168670B1 publication Critical patent/KR0168670B1/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

내용 없음

Description

스트렙토마이세테스내에서의 조절된 유전자 발현
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 플라스미드 pKORE2의 제한지도이다, 제2도는 플라스미드 pKILP1의 제한지도이다.

Claims (4)

  1. 스위치로서 Kil A-Kor A 시스템을 사용하고 발현시킬 유전자를 Kil A 조절 영역과 커플링시키며 스위치 온 되도록 Kor A 단백질을 불활성화시키거나 제거시킴을 특징으로 하는, 스트렙토마이세테스 숙주 균주내에서 유전자의 조절된 발현 방법.
  2. 제1항에 있어서, 온도-민감성 Kor A 단백질을 발현시키고 온도를 이의 역치 이상으로 상승시키는 방법.
  3. 제1항에 있어서,Kor A 유전자를 스위치 가능한 프로모터의 조절하에 있도록 하는 방법.
  4. 제1항에 있어서,Kor A 유전자를 온도-민감성 플라스미드에 의해 발현시키고 온도를 이의 역치 이상으로 상승시키는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019910005820A 1990-04-12 1991-04-12 스트렙토마이세테스내에서의 조절된 유전자 발현 KR0168670B1 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE4011863A DE4011863A1 (de) 1990-04-12 1990-04-12 Regulierte genexpression in streptomyceten
DEP4011863.0 1990-04-12

Publications (2)

Publication Number Publication Date
KR910018552A true KR910018552A (ko) 1991-11-30
KR0168670B1 KR0168670B1 (ko) 1999-01-15

Family

ID=6404289

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019910005820A KR0168670B1 (ko) 1990-04-12 1991-04-12 스트렙토마이세테스내에서의 조절된 유전자 발현

Country Status (13)

Country Link
US (1) US5336607A (ko)
EP (1) EP0451792B1 (ko)
JP (1) JP3043832B2 (ko)
KR (1) KR0168670B1 (ko)
AT (1) ATE150485T1 (ko)
AU (1) AU630551B2 (ko)
DE (2) DE4011863A1 (ko)
DK (1) DK0451792T3 (ko)
ES (1) ES2099101T3 (ko)
GR (1) GR3023115T3 (ko)
IE (1) IE911228A1 (ko)
PT (2) PT97327B (ko)
TW (1) TW226409B (ko)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1352964A4 (en) * 2000-12-22 2005-02-16 Nippon Soda Co PROCESS FOR PRODUCING A SUBSTANCE USING A MICROBIAL CATALYST

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3809692A1 (de) * 1988-03-23 1989-10-12 Hoechst Ag Verwendung von psg5 als temperatursensitives plasmid

Also Published As

Publication number Publication date
EP0451792A1 (de) 1991-10-16
PT97327A (pt) 1992-01-31
KR0168670B1 (ko) 1999-01-15
DE4011863A1 (de) 1991-10-17
US5336607A (en) 1994-08-09
DK0451792T3 (da) 1997-09-15
JPH04228082A (ja) 1992-08-18
DE59108620D1 (de) 1997-04-24
IE911228A1 (en) 1991-10-23
PT97327B (pt) 1998-08-31
ATE150485T1 (de) 1997-04-15
ES2099101T3 (es) 1997-05-16
AU630551B2 (en) 1992-10-29
PT97331A (pt) 1992-01-31
GR3023115T3 (en) 1997-07-30
AU7428791A (en) 1991-10-17
TW226409B (ko) 1994-07-11
EP0451792B1 (de) 1997-03-19
JP3043832B2 (ja) 2000-05-22

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