KR910003380A - 단백질 분해물의 산화 변성법 - Google Patents

단백질 분해물의 산화 변성법 Download PDF

Info

Publication number
KR910003380A
KR910003380A KR1019900010339A KR900010339A KR910003380A KR 910003380 A KR910003380 A KR 910003380A KR 1019900010339 A KR1019900010339 A KR 1019900010339A KR 900010339 A KR900010339 A KR 900010339A KR 910003380 A KR910003380 A KR 910003380A
Authority
KR
South Korea
Prior art keywords
protein
oxidant
reducing agent
oxidation potential
disulfide
Prior art date
Application number
KR1019900010339A
Other languages
English (en)
Inventor
엘팅 제임스
퀼블 하인쯔
Original Assignee
몰레큘라 다이아그노스틱스, 인크
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 몰레큘라 다이아그노스틱스, 인크 filed Critical 몰레큘라 다이아그노스틱스, 인크
Publication of KR910003380A publication Critical patent/KR910003380A/ko

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57473—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving carcinoembryonic antigen, i.e. CEA
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Oncology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hospice & Palliative Care (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)

Abstract

내용 없음.

Description

단백질 분해물의 산화 변성법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도 내지 제3도는 단백질 항원인 CEA의 항원성에 대한 페리오데이트, 페록소디술페이트, 및 하이포클로라이트의 각 효과를 나타내는 그래프임.

Claims (10)

  1. 단백질 내의 디술피드 결합을 절단하기에 충분한 산화 전위를 가진 산화제를 사용하여 단백질을 처리하는 단계, 일정한 반응 시간 후, 생성된 혼합물을 잔류하는 산화제와 반응하여 이를 불활성화시키기에 충분한 양의 환원제와 접촉시키는 단계로 이루어짐을 특징으로 하여, 디술피드 교차결합을 갖는 단백질에 향원성을 부여하거나 증가시키는 방법.
  2. 제1항에 있어서, 산화제가 적어도 약 1.3볼트의 산화 전위를 가지고, 퍼요오데이트, 퍼옥소디술페이트, 하이포클로라이트, 크로메이트, 퍼망가네이트 및 퍼클로레이트 중에서 선택되며, 또한 환원제는 티오술페이트, 디티오나이트, 디티오트레이톨, 디티오에리트리톨 및 메르캅토에탄올 중에서 선택되는 방법.
  3. 제1항 또는 제2항에 있어서, 산화 단계를 약 20℃ 내지 약 70℃ 사이의 온도에서 행하는 방법.
  4. 단백질 내의 디술피드 결합을 절단시키기에 충분한 산화 전위를 갖는 산화제와 단백질을 반응시켜서 이 단백질을 변형시키는 단계와, 생성된 혼합물을 잔류 산화제와 반응하여 이를 불활성화시키기에 충분한 양의 환원제와 접촉시키는 것으로 이루어짐을 특징으로 하는, 단백질을 변성시킨 후 항체 시약과 결합시켜 디술피드 교차결합을 갖는 단백질을 피검시료중에서 검출하는 면역분석법.
  5. 제4항에 있어서, 산화제는 적어도 약 1.3볼트의 산화 전위를 가지고 퍼요오데이트, 퍼옥소디술페이트, 하이포클로라이트, 크로메이트, 퍼망가네이트 및 퍼클로레이트 중에서 선택되며, 환원제는 티오술페이트, 디티오나이트, 디티오트레이톨, 디티오에리트리톨 및 메르캅토에탄올 중에서 선택되는 방법.
  6. (1) 단백질내 선형 펩티드 에피토프에 특이성인 항체 시약, (2) 단백질내의 디술피드 결합을 절단시키기에 충분한 산화 전위를 갖는 산화제, 및 (3) 상기 산화제와 반응하여 이를 불활성화시킬 수 있는 환원제로 이루어짐을 특징으로 하는, 피검시료 중 디술피드 교차결합을 가진 단백질의 면역분석 검출용 시약계.
  7. 제6항에 있어서, 산화제는 적어도 1.3볼트의 산화 전위를 가지며 퍼요오데이트, 퍼옥소디술페이트, 하이포클로라이트, 크로메이트, 퍼망가네이트 및 퍼클로레이트 중에서 선택되고, 또한 환원제는 티오술페이트, 디티오나이트, 디티오트레이톨, 디티오에리트리톨 및 메르캅토에탄올 중에서 선택되는 시약계.
  8. 제6항 또는 7항에 있어서, 상기 항체 시약이, 이종 이뮤노겐 담체에 결합되는 펩티드 잔기를 갖는 합성 펩티드 이뮤노겐에 대한 것인 시약계.
  9. 제1항 내지 4항중 어느 하나의 항에 있어서, 피검 시료가 생물학적 유체인 방법.
  10. 제1항 내지 제4항 중 어느 하나의 항에 있어서, 검출하고자 하는 단백질이 CEA군 중 일원인 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019900010339A 1989-07-10 1990-07-09 단백질 분해물의 산화 변성법 KR910003380A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/377,680 US5061790A (en) 1989-07-10 1989-07-10 Oxidative denaturation of protein analytes
US377680 1989-07-10

Publications (1)

Publication Number Publication Date
KR910003380A true KR910003380A (ko) 1991-02-27

Family

ID=23490110

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019900010339A KR910003380A (ko) 1989-07-10 1990-07-09 단백질 분해물의 산화 변성법

Country Status (18)

Country Link
US (1) US5061790A (ko)
EP (1) EP0407813B1 (ko)
JP (1) JP2915970B2 (ko)
KR (1) KR910003380A (ko)
AT (1) ATE147167T1 (ko)
AU (1) AU633115B2 (ko)
CA (1) CA2019671C (ko)
DE (1) DE69029540T2 (ko)
DK (1) DK0407813T3 (ko)
ES (1) ES2095847T3 (ko)
FI (1) FI903464A0 (ko)
GR (1) GR3022906T3 (ko)
IE (1) IE902493A1 (ko)
IL (1) IL94993A (ko)
NO (1) NO902833L (ko)
NZ (1) NZ234396A (ko)
PT (1) PT94633B (ko)
ZA (1) ZA905360B (ko)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2740223B1 (fr) * 1995-10-19 1997-11-14 Commissariat Energie Atomique Procede de determination d'auto-anticorps revelateurs de complications pathologiques ; dans le serum sanguin, en particulier de diabetiques
US20030202980A1 (en) * 1995-12-29 2003-10-30 Caplan Michael J. Methods and reagents for decreasing clinical reaction to allergy
JP2002501748A (ja) 1998-01-31 2002-01-22 ユニバーシティ オブ アーカンソー アレルギー反応を減少させるための方法および試薬
US7879977B2 (en) 1998-01-31 2011-02-01 University Of Arkansas Methods and reagents for decreasing clinical reaction to allergy
GB9827845D0 (en) * 1998-12-17 1999-02-10 Scolab S A Immunoassay or proteins
US8246945B2 (en) * 2000-04-06 2012-08-21 University Of Arkansas Methods and reagents for decreasing clinical reaction to allergy
EP1272213B1 (en) * 2000-04-06 2006-03-08 SEER Pharmaceuticals, LLC. Microbial delivery system
US20050063994A1 (en) * 2000-04-06 2005-03-24 Caplan Michael J. Methods and reagents for decreasing clinical reaction to allergy
WO2003002908A1 (en) * 2000-06-26 2003-01-09 Reveo, Inc. Backlight for a liquid crystal display having high light-recycling efficiency
DE10054055A1 (de) 2000-10-31 2002-05-23 Nmi Univ Tuebingen Verfahren zur Analyse von Proteinen
US7618788B2 (en) * 2002-05-10 2009-11-17 Millipore Corporation Proteome epitope tags and methods of use thereof in protein modification analysis
US7460960B2 (en) * 2002-05-10 2008-12-02 Epitome Biosystems, Inc. Proteome epitope tags and methods of use thereof in protein modification analysis
CN1832756A (zh) 2003-06-09 2006-09-13 约翰·A·麦金太尔 通过氧化还原反应改变血浆蛋白质结合专一性的方法
US7892751B2 (en) * 2003-06-09 2011-02-22 Redox-Reactive Reagents Llc Method of detecting or diagnosing of a neurodegenerative disease or condition
US9557325B2 (en) * 2003-06-09 2017-01-31 Redox-Reactive Reagents Llc Method of altering the binding specificity of proteins by oxidation-reduction reactions
US7855057B2 (en) * 2006-03-23 2010-12-21 Millipore Corporation Protein splice variant/isoform discrimination and quantitative measurements thereof
JP5081930B2 (ja) * 2007-03-08 2012-11-28 エフ．ホフマン−ラ ロシュ アーゲー 心不全の評価におけるｓｌｉｍ−１の使用
KR101322247B1 (ko) * 2007-09-06 2013-10-25 삼성전자주식회사 진공청소기용 흡입브러시 및 그 높이조절방법
US20090280474A1 (en) * 2008-05-08 2009-11-12 Abbott Laboratories Method for detecting a virus
US20130189707A1 (en) * 2010-01-26 2013-07-25 Svetlana Alexandrovna Sergeeva Method of determination of autoantibody level by means of enzyme immunoassay
RU2014135576A (ru) * 2012-02-02 2016-03-27 Перфинити Байосайенсиз, Инк. Распознавание на основе селектора и системы количественной оценки, а также способ единого анализа множества анализируемых веществ

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2448530C3 (de) * 1974-10-11 1980-03-27 Behringwerke Ag, 3550 Marburg Verfahren zum Herstellen von Derivaten des Diphtherietoxins und diese enthaltende Mittel
US4658022A (en) * 1985-08-08 1987-04-14 Molecular Diagnostics, Inc. Binding of antibody reagents to denatured protein analytes
US4683136A (en) * 1985-03-06 1987-07-28 Scripps Clinic And Research Foundation Proteinaceous antigens with conformation-independent and conformation-dependent determinants
US4713366A (en) * 1985-12-04 1987-12-15 The Ohio State University Research Foundation Antigenic modification of polypeptides

Also Published As

Publication number Publication date
DE69029540T2 (de) 1997-05-07
GR3022906T3 (en) 1997-06-30
NO902833D0 (no) 1990-06-26
IE902493A1 (en) 1991-02-13
PT94633A (pt) 1991-03-20
DK0407813T3 (da) 1997-06-16
JP2915970B2 (ja) 1999-07-05
DE69029540D1 (de) 1997-02-13
EP0407813A2 (en) 1991-01-16
IL94993A0 (en) 1991-06-10
CA2019671C (en) 2002-08-13
FI903464A0 (fi) 1990-07-09
ES2095847T3 (es) 1997-03-01
NO902833L (no) 1991-01-11
AU633115B2 (en) 1993-01-21
EP0407813B1 (en) 1997-01-02
JPH0368868A (ja) 1991-03-25
AU5872790A (en) 1991-01-10
NZ234396A (en) 1992-02-25
IL94993A (en) 1994-05-30
PT94633B (pt) 1997-04-30
ATE147167T1 (de) 1997-01-15
ZA905360B (en) 1991-04-24
CA2019671A1 (en) 1991-01-10
US5061790A (en) 1991-10-29
EP0407813A3 (en) 1992-11-25

Similar Documents

Publication Publication Date Title
KR910003380A (ko) 단백질 분해물의 산화 변성법
ES2148225T3 (es) Reactivos y procedimientos que permiten la deteccion y la cuantificacion de la tiroxina en muestras de fluido.
KR890701632A (ko) 사람 인터루킨-4에 대한 모노클로날 항체 및 이를 생성하는 하이브리도마
EP1104887A3 (en) A method and test kit for assaying collagen fragments in body fluids
DE3750354T2 (de) Monoklonale Antikörper und Verfahren zum Nachweis von Dioxinen und Dibenzofuranen.
DE3883073D1 (de) Einschritt-immuntest zur bestimmung von antigen-spezifischen antikoerpern aus einer der immunglobulin-klassen a, m, d oder e und dazu geeignetes mittel.
Grey Duck Immunoglobulins: I. Structural Studies on a 5.7 S and 7.8 S γ-Globulin
DE59509344D1 (de) Antikörperklassenspezifisches entstörungsreagenz
DE69230917D1 (de) Verfahren zur detektion von hepatitis c
AU566742B2 (en) Immunologic latex agglutination process
Alexandru et al. Non-covalent association of heavy and light chains of human immunoglobulin G: studies using light chain labelled with a fluorescent probe
KR930008155A (ko) 씨형 간염 바이러스(hcv)에 대한 모노클로날 항체
DE69112225D1 (de) Immunodiagnostische probe für gelenkrheumatismus.
ES2168310T3 (es) Reactivos y metodos de deteccion y de cuantificacion de la testosterona en muestras de fluido.
DK515889D0 (da) Hidtil ukendt fremgangsmaade
Fuller et al. Isolation from nurse shark serum of immune 7S antibodies with two different molecular weight H-chains
KR930703613A (ko) 시료 희석물중 과산화물 디스뮤타제의 용도
KR900006364A (ko) Hiv-항체와 면역 화학적으로 반응하는 합성펩티드
DE68917797T2 (de) Gegen menschliches Fibrinopepid A spezifische monoklonale Antikörper.
Hurst et al. A comparative analysis of the C1-binding ability of fragments derived from complement-fixing and noncomplement-fixing IgM proteins.
DE58909219D1 (de) Einschritt-Immuntest zur Bestimmung von antigen-spezifischen Antikörpern aller Immunglobulin-Klassen und dazu geeignetes Mittel.
KR900003204A (ko) Hiv-항체와 면역화학적으로 반응성 있는 합성 펩티드
McBurnette et al. Rabbit IgG hinge region: site of group d allotypic specificity
Malchy Haptoglobins and hemoglobin-haptoglobin complexes
DK0497886T3 (da) Assay, der omfatter blandede immunoglobuliner til påvisning af rheumatoide faktorer

Legal Events

Date Code Title Description
A201 Request for examination
N231 Notification of change of applicant
E902 Notification of reason for refusal
E601 Decision to refuse application