KR930703613A - 시료 희석물중 과산화물 디스뮤타제의 용도 - Google Patents
시료 희석물중 과산화물 디스뮤타제의 용도Info
- Publication number
- KR930703613A KR930703613A KR1019930702190A KR930702190A KR930703613A KR 930703613 A KR930703613 A KR 930703613A KR 1019930702190 A KR1019930702190 A KR 1019930702190A KR 930702190 A KR930702190 A KR 930702190A KR 930703613 A KR930703613 A KR 930703613A
- Authority
- KR
- South Korea
- Prior art keywords
- peroxide dismutase
- sample
- diluent
- dismutase
- peroxide
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/576—Immunoassay; Biospecific binding assay; Materials therefor for hepatitis
- G01N33/5767—Immunoassay; Biospecific binding assay; Materials therefor for hepatitis non-A, non-B hepatitis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Communicable Diseases (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
본 발명은 항체를 검출하는 개선된 방법에 관한 것이다. 이 방법은 (a) 시료를 과산화물 디스뮤타제를 포함하는 희석물과 혼합하고, (b) 희석된 시료를 과산화물 디스큐타제와의 융합 단백질로서 발현된 제조합체 항원 하나 이상과 접촉시키는 단계를 수반한다.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 HCV 그릇된 양성 시료군에 SOD를 첨가한 효과를 나타낸다.
Claims (8)
- (a)시료를 과산화물 디스뮤타제를 포함하는 희석물과 혼합하고, (b)희석된 시료를 과산화물 디스뮤타제와의 융합 단백질로서 발현된 제조합체 항원 하나 이상과 접촉시키는 단계를 포함함을 특징으로 하며, 시료중의 항체를 검출하는 방법.
- 제1항에 있어서, 과산화물 디스뮤타제가 제조합체 단백질인 방법.
- 제1항에 있어서, 과산화물 디스뮤타제의 농도가 희석물 1ℓ당 약 0.001g내지 약 1.0g인 방법.
- 제1항에 있어서, 과산화물 디스뮤타제의 농도가 희석물 1ℓ당 약 0.01g내지 약 0.1g인 방법.
- 제1항에 있어서, 항-HCV 항체를 검출하는 방법.
- 제1항에 있어서, 제조합체 항원이 SOD-c100-3인 방법.
- (a)시료를 과산화물 디스뮤타제를 포함하는 희석물과 혼합하고, (b)희석된 시료를 과산화물 디스뮤타제와의 융합 단백질로서 발현된 재조합체 항원 하나 이상과 접촉시키는 단계를 포함함을 특징으로하여, 시료중의 항-HCV 항체를 검출하는 방법.
- 제7항에 있어서, 재조합체 항원이 SOD-c100-3인 방법.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US64737491A | 1991-01-25 | 1991-01-25 | |
US07/647,374 | 1991-01-25 | ||
PCT/US1992/000604 WO1992013275A1 (en) | 1991-01-25 | 1992-01-24 | Use of superoxide dismutase in specimen diluent |
Publications (1)
Publication Number | Publication Date |
---|---|
KR930703613A true KR930703613A (ko) | 1993-11-30 |
Family
ID=24596744
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019930702190A KR930703613A (ko) | 1991-01-25 | 1992-01-04 | 시료 희석물중 과산화물 디스뮤타제의 용도 |
Country Status (8)
Country | Link |
---|---|
US (1) | US5330893A (ko) |
EP (1) | EP0568639A4 (ko) |
JP (1) | JPH06506770A (ko) |
KR (1) | KR930703613A (ko) |
AU (1) | AU658395B2 (ko) |
CA (1) | CA2101056A1 (ko) |
TW (1) | TW213981B (ko) |
WO (1) | WO1992013275A1 (ko) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2803020B2 (ja) * | 1994-05-06 | 1998-09-24 | ダイナボット株式会社 | Hiv関連抗体測定法 |
JP2803019B2 (ja) * | 1994-05-06 | 1998-09-24 | ダイナボット株式会社 | Hcv関連抗体測定法 |
AU6946394A (en) * | 1994-05-09 | 1995-11-29 | Abbott Laboratories | Method and reagents useful for improving immunoassay specificity |
US6511812B1 (en) * | 1994-05-09 | 2003-01-28 | Abbott Laboratories | Method and test kit for use in improving immunoassay specificity |
BR9906660A (pt) | 1998-07-30 | 2000-08-29 | Advanced Life Science Inst Inc | Processo para medir vìrus da hepatite c |
CA2824758C (en) | 2011-01-13 | 2019-06-04 | Ortho-Clinical Diagnostics, Inc. | Treponema pallidum triplet antigen |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4940659A (en) * | 1987-02-20 | 1990-07-10 | Monoclonetics International, Inc. | Screening extra-cellular body fluids for superoxide dismutase (SOD-1) for determining fetal trisomy 21 down syndrome |
EP0318216B2 (en) * | 1987-11-18 | 2001-08-29 | Chiron Corporation | NANBV diagnostics |
US5147783A (en) * | 1988-05-27 | 1992-09-15 | Ube Industries, Ltd. | Methods to screen for ovarian cancer and myocardial infarction |
-
1992
- 1992-01-04 KR KR1019930702190A patent/KR930703613A/ko not_active Application Discontinuation
- 1992-01-04 CA CA002101056A patent/CA2101056A1/en not_active Abandoned
- 1992-01-24 JP JP4505708A patent/JPH06506770A/ja active Pending
- 1992-01-24 WO PCT/US1992/000604 patent/WO1992013275A1/en not_active Application Discontinuation
- 1992-01-24 EP EP19920905916 patent/EP0568639A4/en not_active Withdrawn
- 1992-01-24 AU AU13354/92A patent/AU658395B2/en not_active Ceased
- 1992-01-25 TW TW081100585A patent/TW213981B/zh active
-
1993
- 1993-05-10 US US08/059,868 patent/US5330893A/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
AU1335492A (en) | 1992-08-27 |
EP0568639A1 (en) | 1993-11-10 |
TW213981B (ko) | 1993-10-01 |
JPH06506770A (ja) | 1994-07-28 |
CA2101056A1 (en) | 1992-07-26 |
WO1992013275A1 (en) | 1992-08-06 |
EP0568639A4 (en) | 1994-06-29 |
US5330893A (en) | 1994-07-19 |
AU658395B2 (en) | 1995-04-13 |
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Legal Events
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WITN | Application deemed withdrawn, e.g. because no request for examination was filed or no examination fee was paid |