KR900702025A - 형질 변환된 시겔라(Shigella) - Google Patents

형질 변환된 시겔라(Shigella)

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Publication number
KR900702025A
KR900702025A KR1019900700567A KR900700567A KR900702025A KR 900702025 A KR900702025 A KR 900702025A KR 1019900700567 A KR1019900700567 A KR 1019900700567A KR 900700567 A KR900700567 A KR 900700567A KR 900702025 A KR900702025 A KR 900702025A
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Prior art keywords
shigella
gene
host
genome
genes
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KR1019900700567A
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English (en)
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필립 셍소네띠
에니크 퐁테인
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엥스뛰띠 파스떼르
원본미기재
엥스뛰띠 나쇼날 드 라 상떼 에 드 라 러세르 메디칼
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Priority claimed from EP88401842.5A external-priority patent/EP0350555A1/en
Application filed by 엥스뛰띠 파스떼르, 원본미기재, 엥스뛰띠 나쇼날 드 라 상떼 에 드 라 러세르 메디칼 filed Critical 엥스뛰띠 파스떼르
Publication of KR900702025A publication Critical patent/KR900702025A/ko

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/025—Enterobacteriales, e.g. Enterobacter
    • A61K39/0283—Shigella
    • A—HUMAN NECESSITIES
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    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • A61P31/04—Antibacterial agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/25—Shigella (G)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/52—Bacterial cells; Fungal cells; Protozoal cells
    • A61K2039/522—Bacterial cells; Fungal cells; Protozoal cells avirulent or attenuated
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    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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Abstract

내용 없음

Description

형질 변환된 시겔라(Shigella)
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
첨부한 도면은 시가-독소 오페론의 클로닝 및 실시예 2의 시가-독소 A 소단위(subunit)유전자의 시험관내 돌연변이를 도식적으로 나타내고 있다. 도면상의 플라즈미드 pHS7201, pHS7202 및 pHS7203에 있어서 굵은선은 A 소단위 유전자의 서열을 나타내며; 사선은 B 소단위 유전자의 서열을 나타내며삽입 부분으로 부터의 서열을 나타낸다.

Claims (13)

  1. 숙주 세포에 거의 침투할 수 없으며, 감염 세포내에서 및 숙주의 감염 세포로 부터 비감염 세포로 거의 확산될 수 없고, 숙주의 비감염 세포뿐만 아니라 감염 세포를 다량 죽일 수 있는 독소를 생성하지 못하도록 야생주 시겔라의 게놈을 형질 변환시키는 단계를 특징으로 하는 야생주 시겔라에 대한 왁찐을 제조하는데 사용할 수 있는 시겔라의 변이주를 생성하기 위한 장침투성 시겔라 야생주의 변이 방법.
  2. 제1항에 있어서, 시겔라의 야생주가 숙주의 조직뿐만 아니라, 세포에 침투하는데 필요한 단백질을 코오딩한 제1유전자와, 시겔라의 야생주가 숙주의 감염 세포와 비감염 세포 사이 및 감염 세포내에서 확산되는데 필요한 단백질을 코오딩한 제2유전자를 완전히 또는 부분적으로 제거하거나 영구적으로 불활성화 시킬수 있도록 시겔라 야생주의 게놈을 변이시키는 방법.
  3. 제2항에 있어서, 시겔라가 s. 플렉스너이고 제1유전자는 s. 플렉스너에 의한 에어로박틴의 사용 또는 생성을 코오드하는 방법.
  4. 제3항에 있어서, 제2유전자가 세포간-세포내 확산을 위해 코오드하는 방법.
  5. 제2항에 있어서, 시겔라는s.이질1 이고,s.이질1 에 의해 시가-독소의 사용 또는 생성을 코오드하는 제3유전자가 부분적으로 또는 완전히 제거되거나 영구적으로 불활성화시킬 수 있도록 게놈을 변이시키는 방법.
  6. 제5항에 있어서,s.이질1 의 제1유전자가s.이질1 에 의한 엔테로켈린의 사용 또는 생성을 코오드하는 방법.
  7. 제6항에 있어서, 제2유전자가 세포간-세포내 확산을 위해 코오드하는 방법.
  8. 제6항에 있어서, 제1유전자가s.이질1 의 엔테로켈린의 오페론의 ent F, Fep E, Fep C 및 Fep D 소단위 유전자를 포함한 방법.
  9. 제5항 내지 제8항중 어느 한 항에 있어서, 제1, 제2 및 제3유전자가 변이된 방법.
  10. 제2항 내지 제9항중 어느 한 항에 있어서, 하나 이상의 유전자가 하나 이상의 시험관내 변이 유전자를 이용한 대립형질 교환에 의해 불활성화된 방법으로서 특히 상기 변이 유전자는 중요 부위가 누락되었으며 마커 유전자가 그 변이 유전자로 삽입된 방법.
  11. 제1항 내지 제10항중 어느 한 항의 방법에 의해 변이되거나 그것의 후세대인 시겔라.
  12. 제11항의 변이된 시겔라로 부터 제조된 왁찐.
  13. 시가-독소 특히 시가-독소 A인 시겔라.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019900700567A 1988-07-15 1989-07-14 형질 변환된 시겔라(Shigella) KR900702025A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP88401842.5 1988-07-15
EP88401842.5A EP0350555A1 (en) 1988-07-15 Transformed shigella
PCT/EP1989/000831 WO1990000604A1 (en) 1988-07-15 1989-07-14 Transformed shigella

Publications (1)

Publication Number Publication Date
KR900702025A true KR900702025A (ko) 1990-12-05

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ID=8200405

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Application Number Title Priority Date Filing Date
KR1019900700567A KR900702025A (ko) 1988-07-15 1989-07-14 형질 변환된 시겔라(Shigella)

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Country Link
US (2) US7138126B2 (ko)
EP (1) EP0351322B1 (ko)
JP (3) JP3608742B2 (ko)
KR (1) KR900702025A (ko)
AR (1) AR242989A1 (ko)
AT (1) ATE106941T1 (ko)
AU (1) AU620630B2 (ko)
CA (1) CA1324970C (ko)
DE (1) DE68915876T2 (ko)
DK (1) DK175464B1 (ko)
ES (1) ES2054058T3 (ko)
OA (1) OA09440A (ko)
PT (1) PT91177B (ko)
WO (1) WO1990000604A1 (ko)
ZA (1) ZA895371B (ko)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AR242989A1 (es) * 1988-07-15 1993-06-30 Inst Pasteur I Nat De La Sante Metodo para modificar una cepa salvaje de una shigella entero-invasora para producir una cepa modificada apta para preparar una vacuna contra la cepa salvaje y cepa de shigella asi modificada.
ATE111957T1 (de) * 1990-02-06 1994-10-15 Pasteur Institut Transformiertes shigella.
DE69221120T2 (de) * 1992-04-10 1998-02-26 Schweiz Serum & Impfinst Rekombinanter lebender Impfstoff gegen Gram-negative enterische Pathogene
IL123569A (en) * 1995-09-06 2006-10-05 Dept Of The Army Attenuated shigella strain for delivering a mamalian expression plasmid into a mammalian cell
BRPI0816842A2 (pt) * 2007-04-25 2019-09-24 The Us Secretary Of The Army Walter Reed Army Insitute Of Research cepa de vacina de shigella, composição imunogênica, vacina, kit para o tratamento de um indivíduo e método.

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4550081A (en) * 1980-05-19 1985-10-29 The Board Of Trustees Of The Leland Stanford Jr. University Non-reverting salmonella
US5762941A (en) * 1988-07-15 1998-06-09 Institut Pasteur Modified shigella having reduced pathogenicity
AR242989A1 (es) * 1988-07-15 1993-06-30 Inst Pasteur I Nat De La Sante Metodo para modificar una cepa salvaje de una shigella entero-invasora para producir una cepa modificada apta para preparar una vacuna contra la cepa salvaje y cepa de shigella asi modificada.

Also Published As

Publication number Publication date
JP2005013234A (ja) 2005-01-20
OA09440A (en) 1992-10-15
US20050031644A1 (en) 2005-02-10
CA1324970C (en) 1993-12-07
ATE106941T1 (de) 1994-06-15
DK175464B1 (da) 2004-11-01
WO1990000604A1 (en) 1990-01-25
JP2006280378A (ja) 2006-10-19
JP4277957B2 (ja) 2009-06-10
EP0351322B1 (en) 1994-06-08
PT91177A (pt) 1990-02-08
US7138126B2 (en) 2006-11-21
DK65790D0 (da) 1990-03-13
PT91177B (pt) 1995-09-12
DK65790A (da) 1990-03-13
US7439053B2 (en) 2008-10-21
ES2054058T3 (es) 1994-08-01
AR242989A1 (es) 1993-06-30
US20060257940A1 (en) 2006-11-16
JP3608742B2 (ja) 2005-01-12
DE68915876T2 (de) 1994-09-22
EP0351322A1 (en) 1990-01-17
JPH03500368A (ja) 1991-01-31
AU3879589A (en) 1990-02-05
AU620630B2 (en) 1992-02-20
ZA895371B (en) 1990-04-25
DE68915876D1 (de) 1994-07-14

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