KR900700623A - 감염증 진단방법 및 미생물의 검출과 미생물의 동정방법 - Google Patents

감염증 진단방법 및 미생물의 검출과 미생물의 동정방법

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KR900700623A
KR900700623A KR1019890702398A KR890702398A KR900700623A KR 900700623 A KR900700623 A KR 900700623A KR 1019890702398 A KR1019890702398 A KR 1019890702398A KR 890702398 A KR890702398 A KR 890702398A KR 900700623 A KR900700623 A KR 900700623A
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sample
specimen
radioactive
bacteria
following
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KR1019890702398A
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KR930000277B1 (ko
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쓰네야 오오노
아키오 마쓰히사
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쓰네야 오오노
도다 미키오
후소야쿠힝코교 가부시키가이샤
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
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    • Y10S435/8215—Microorganisms
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    • Y10S435/973—Simultaneous determination of more than one analyte

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

내용 없음

Description

감염증 진단방법 및 미생물의 검출과 미생물의 동정방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 플레이트 1에 있어서, 사람 세포중의 임균(임자상 시그날)을 나타낸다.
제2도는 플레이트 2에 있어서, 사람 백혈구중에 포획된 균(동정되지 않은 것) (임상시험)을 나타낸다. 제3도는 플레이트 3에 있어서, 사람 백혈구중에 포획된 포도상 구균(in Vitro)을 나타나낸다.

Claims (5)

  1. 다음 (Ⅰ) 및 (Ⅱ)의 단계를 포함하는 감염중의 진단 시스템. (Ⅰ) 생체성분으로서 혈액, 복수, 그밖의 식세포를 다량 함유하는 분획 또는 성분인 검체를 고정시킨다. (Ⅱ) 다음 ①의 단계를 포함하는 방법으로 검체중의 균을 동정한다. ① 방사성 또는 비방사성 어느 한 프로브를 사용하여 검체에 하이브리다이제이션을 실시한다.
  2. 다음 (Ⅰ) 내지 (Ⅲ)의 단계를 포함하는 감염증의 진단시스템. (Ⅰ) 생체성분으로서 혈액, 복수, 그밖의 식세포를 다량 함유하는 분획 또는 성분인 검체를 고정시킨다. (Ⅲ) 다음 ① 및 ②의 단계를 포함하는 방법으로 검체중의 균을 검출한다. ① 검체에 스트렙트아비딘(Streptavidin)을 함유하는 아비딘상 단백질을 접촉시킨다. ① 상기의 처리를 실시한 검체에 효소, 항체, 색소 및 금 등의 표지분자를 결합시킨 비오틴을 접촉시킨다. (Ⅲ) 상기 (Ⅱ)에서 균이 검출된 검체에 대하여, 다음 ①의 단계를 포함하는 방법으로 검체중의 균을 동정한다. ① 방사성 또는 비방사성 어느 한 프로브를 사용하여 검출에 하이브리다이제이션을 실시한다.
  3. 다음 (Ⅰ) 내지 (ⅱ)의 단계를 포함하는, 검체중의 균을 검출하는 방법. (Ⅰ) 생체성분으로서 혈액, 복수, 그밖의 식세포를 다량 함유하는 분획 또는 성분인 검체를 고정시킨다. (Ⅱ) 다음 ①의 단계를 포함하는 방법으로 검체중의 균을 동정한다. ① 방사성 또는 비방사성 어느 한 프로브를 사용하여 검체에 하이브리다이제이션을 실시한다.
  4. 다음 (Ⅰ) 내지 (Ⅱ)의 단계를 포함하는, 검체중의 균을 검출하는 방법. (Ⅰ) 생체성분으로서 혈액, 복수, 그밖의 식세포를 다량 함유하는 분획 또는 성분인 검체를 고정시킨다. (Ⅱ) 검체에 스트렙트아비딘을 포함하는 아비딘상 단백질을 접촉시킨다. (Ⅰ) 상기의 처리를 실시한 검체에 효소, 항체, 색소, 금 등의 표지분자를 결합시킨 비오틴을 접촉시킨다.
  5. 다음 (Ⅰ) 내지 (Ⅱ)의 단계를 포함하는, 검체중의 균을 동정하는 방법. (Ⅰ) 생체성분으로서 혈액, 복수, 그밖의 식세포를 다량 함유하는 분획 또는 성분인 검체를 고정시킨다. (Ⅱ) 방사성 또는 비방사성 어느 한 프로브를 사용하여 검체에 하이브리다이제이션을 실시한다.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019890702398A 1988-04-20 1989-04-19 감염증 병균균의 검출 및 동정방법 KR930000277B1 (ko)

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Application Number Priority Date Filing Date Title
JP97,808/88 1988-04-20
JP9780888 1988-04-20
JP97808/88 1988-04-20
PCT/JP1989/000424 WO1989010411A1 (en) 1988-04-20 1989-04-19 Method for diagnosis of infectious diseases and method of detecting and identifying microorganisms

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KR900700623A true KR900700623A (ko) 1990-08-16
KR930000277B1 KR930000277B1 (ko) 1993-01-14

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US (1) US5358846A (ko)
EP (1) EP0366813B1 (ko)
KR (1) KR930000277B1 (ko)
AT (1) ATE133205T1 (ko)
AU (1) AU623889B2 (ko)
DE (1) DE68925457T2 (ko)
TW (1) TW211039B (ko)
WO (1) WO1989010411A1 (ko)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2663228A2 (fr) * 1989-09-29 1991-12-20 Agronomique Inst Nat Rech Composition immunisante contre la maladie de newcastle et procede de preparation.
DK1498496T3 (da) * 1992-07-07 2006-09-18 Fuso Pharmaceutical Ind DNA-probe, der er specifik for Staphylococcus epidermidis
EP0769068B1 (en) * 1994-06-24 2005-10-12 Innogenetics N.V. Simultaneous detection, identification and differentiation of eubacterial taxa using a hybridization assay
AU2002308892B2 (en) * 2001-05-31 2007-11-01 Fuso Pharmaceutical Industries, Ltd. Method of evaluating the function of phagocyte and utilization thereof
ATE366824T1 (de) * 2001-05-31 2007-08-15 Fuso Pharmaceutical Ind Verbessertes verfahren zum nachweis und zur identifizierung eines eine infektion verursachenden mikroorganismus
CN1918305B (zh) * 2004-02-09 2010-12-01 扶桑药品工业株式会社 核酸检测方法及其应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4711955A (en) * 1981-04-17 1987-12-08 Yale University Modified nucleotides and methods of preparing and using same
US4689295A (en) * 1983-01-20 1987-08-25 Integrated Genetics, Inc. Test for Salmonella
FR2596774B1 (fr) * 1986-04-04 1988-07-22 Pasteur Institut Sondes oligonucleotidiques et procedes pour la detection par hybridation des acides nucleiques de bacteries et autres etres vivants
AU7237587A (en) * 1987-03-24 1988-11-02 Arlin C. Lewis Electrochemical activation of chemical reactions
US4886741A (en) * 1987-12-09 1989-12-12 Microprobe Corporation Use of volume exclusion agents for the enhancement of in situ hybridization

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AU3535389A (en) 1989-11-24
AU623889B2 (en) 1992-05-28
EP0366813A4 (en) 1991-01-30
WO1989010411A1 (en) 1989-11-02
KR930000277B1 (ko) 1993-01-14
TW211039B (ko) 1993-08-11
ATE133205T1 (de) 1996-02-15
DE68925457D1 (de) 1996-02-29
EP0366813A1 (en) 1990-05-09
DE68925457T2 (de) 1996-06-13
US5358846A (en) 1994-10-25
EP0366813B1 (en) 1996-01-17

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