KR900700623A - 감염증 진단방법 및 미생물의 검출과 미생물의 동정방법 - Google Patents
감염증 진단방법 및 미생물의 검출과 미생물의 동정방법Info
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- KR900700623A KR900700623A KR1019890702398A KR890702398A KR900700623A KR 900700623 A KR900700623 A KR 900700623A KR 1019890702398 A KR1019890702398 A KR 1019890702398A KR 890702398 A KR890702398 A KR 890702398A KR 900700623 A KR900700623 A KR 900700623A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6841—In situ hybridisation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/911—Microorganisms using fungi
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/973—Simultaneous determination of more than one analyte
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Toxicology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
내용 없음
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 플레이트 1에 있어서, 사람 세포중의 임균(임자상 시그날)을 나타낸다.
제2도는 플레이트 2에 있어서, 사람 백혈구중에 포획된 균(동정되지 않은 것) (임상시험)을 나타낸다. 제3도는 플레이트 3에 있어서, 사람 백혈구중에 포획된 포도상 구균(in Vitro)을 나타나낸다.
Claims (5)
- 다음 (Ⅰ) 및 (Ⅱ)의 단계를 포함하는 감염중의 진단 시스템. (Ⅰ) 생체성분으로서 혈액, 복수, 그밖의 식세포를 다량 함유하는 분획 또는 성분인 검체를 고정시킨다. (Ⅱ) 다음 ①의 단계를 포함하는 방법으로 검체중의 균을 동정한다. ① 방사성 또는 비방사성 어느 한 프로브를 사용하여 검체에 하이브리다이제이션을 실시한다.
- 다음 (Ⅰ) 내지 (Ⅲ)의 단계를 포함하는 감염증의 진단시스템. (Ⅰ) 생체성분으로서 혈액, 복수, 그밖의 식세포를 다량 함유하는 분획 또는 성분인 검체를 고정시킨다. (Ⅲ) 다음 ① 및 ②의 단계를 포함하는 방법으로 검체중의 균을 검출한다. ① 검체에 스트렙트아비딘(Streptavidin)을 함유하는 아비딘상 단백질을 접촉시킨다. ① 상기의 처리를 실시한 검체에 효소, 항체, 색소 및 금 등의 표지분자를 결합시킨 비오틴을 접촉시킨다. (Ⅲ) 상기 (Ⅱ)에서 균이 검출된 검체에 대하여, 다음 ①의 단계를 포함하는 방법으로 검체중의 균을 동정한다. ① 방사성 또는 비방사성 어느 한 프로브를 사용하여 검출에 하이브리다이제이션을 실시한다.
- 다음 (Ⅰ) 내지 (ⅱ)의 단계를 포함하는, 검체중의 균을 검출하는 방법. (Ⅰ) 생체성분으로서 혈액, 복수, 그밖의 식세포를 다량 함유하는 분획 또는 성분인 검체를 고정시킨다. (Ⅱ) 다음 ①의 단계를 포함하는 방법으로 검체중의 균을 동정한다. ① 방사성 또는 비방사성 어느 한 프로브를 사용하여 검체에 하이브리다이제이션을 실시한다.
- 다음 (Ⅰ) 내지 (Ⅱ)의 단계를 포함하는, 검체중의 균을 검출하는 방법. (Ⅰ) 생체성분으로서 혈액, 복수, 그밖의 식세포를 다량 함유하는 분획 또는 성분인 검체를 고정시킨다. (Ⅱ) 검체에 스트렙트아비딘을 포함하는 아비딘상 단백질을 접촉시킨다. (Ⅰ) 상기의 처리를 실시한 검체에 효소, 항체, 색소, 금 등의 표지분자를 결합시킨 비오틴을 접촉시킨다.
- 다음 (Ⅰ) 내지 (Ⅱ)의 단계를 포함하는, 검체중의 균을 동정하는 방법. (Ⅰ) 생체성분으로서 혈액, 복수, 그밖의 식세포를 다량 함유하는 분획 또는 성분인 검체를 고정시킨다. (Ⅱ) 방사성 또는 비방사성 어느 한 프로브를 사용하여 검체에 하이브리다이제이션을 실시한다.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP97,808/88 | 1988-04-20 | ||
JP9780888 | 1988-04-20 | ||
JP97808/88 | 1988-04-20 | ||
PCT/JP1989/000424 WO1989010411A1 (en) | 1988-04-20 | 1989-04-19 | Method for diagnosis of infectious diseases and method of detecting and identifying microorganisms |
Publications (2)
Publication Number | Publication Date |
---|---|
KR900700623A true KR900700623A (ko) | 1990-08-16 |
KR930000277B1 KR930000277B1 (ko) | 1993-01-14 |
Family
ID=14202067
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019890702398A KR930000277B1 (ko) | 1988-04-20 | 1989-04-19 | 감염증 병균균의 검출 및 동정방법 |
Country Status (8)
Country | Link |
---|---|
US (1) | US5358846A (ko) |
EP (1) | EP0366813B1 (ko) |
KR (1) | KR930000277B1 (ko) |
AT (1) | ATE133205T1 (ko) |
AU (1) | AU623889B2 (ko) |
DE (1) | DE68925457T2 (ko) |
TW (1) | TW211039B (ko) |
WO (1) | WO1989010411A1 (ko) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2663228A2 (fr) * | 1989-09-29 | 1991-12-20 | Agronomique Inst Nat Rech | Composition immunisante contre la maladie de newcastle et procede de preparation. |
DK1498496T3 (da) * | 1992-07-07 | 2006-09-18 | Fuso Pharmaceutical Ind | DNA-probe, der er specifik for Staphylococcus epidermidis |
EP0769068B1 (en) * | 1994-06-24 | 2005-10-12 | Innogenetics N.V. | Simultaneous detection, identification and differentiation of eubacterial taxa using a hybridization assay |
AU2002308892B2 (en) * | 2001-05-31 | 2007-11-01 | Fuso Pharmaceutical Industries, Ltd. | Method of evaluating the function of phagocyte and utilization thereof |
ATE366824T1 (de) * | 2001-05-31 | 2007-08-15 | Fuso Pharmaceutical Ind | Verbessertes verfahren zum nachweis und zur identifizierung eines eine infektion verursachenden mikroorganismus |
CN1918305B (zh) * | 2004-02-09 | 2010-12-01 | 扶桑药品工业株式会社 | 核酸检测方法及其应用 |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4711955A (en) * | 1981-04-17 | 1987-12-08 | Yale University | Modified nucleotides and methods of preparing and using same |
US4689295A (en) * | 1983-01-20 | 1987-08-25 | Integrated Genetics, Inc. | Test for Salmonella |
FR2596774B1 (fr) * | 1986-04-04 | 1988-07-22 | Pasteur Institut | Sondes oligonucleotidiques et procedes pour la detection par hybridation des acides nucleiques de bacteries et autres etres vivants |
AU7237587A (en) * | 1987-03-24 | 1988-11-02 | Arlin C. Lewis | Electrochemical activation of chemical reactions |
US4886741A (en) * | 1987-12-09 | 1989-12-12 | Microprobe Corporation | Use of volume exclusion agents for the enhancement of in situ hybridization |
-
1989
- 1989-04-19 EP EP89905203A patent/EP0366813B1/en not_active Expired - Lifetime
- 1989-04-19 US US07/449,931 patent/US5358846A/en not_active Expired - Lifetime
- 1989-04-19 WO PCT/JP1989/000424 patent/WO1989010411A1/ja active IP Right Grant
- 1989-04-19 DE DE68925457T patent/DE68925457T2/de not_active Expired - Lifetime
- 1989-04-19 KR KR1019890702398A patent/KR930000277B1/ko not_active IP Right Cessation
- 1989-04-19 AU AU35353/89A patent/AU623889B2/en not_active Expired
- 1989-04-19 AT AT89905203T patent/ATE133205T1/de not_active IP Right Cessation
-
1990
- 1990-03-09 TW TW078109185A patent/TW211039B/zh active
Also Published As
Publication number | Publication date |
---|---|
AU3535389A (en) | 1989-11-24 |
AU623889B2 (en) | 1992-05-28 |
EP0366813A4 (en) | 1991-01-30 |
WO1989010411A1 (en) | 1989-11-02 |
KR930000277B1 (ko) | 1993-01-14 |
TW211039B (ko) | 1993-08-11 |
ATE133205T1 (de) | 1996-02-15 |
DE68925457D1 (de) | 1996-02-29 |
EP0366813A1 (en) | 1990-05-09 |
DE68925457T2 (de) | 1996-06-13 |
US5358846A (en) | 1994-10-25 |
EP0366813B1 (en) | 1996-01-17 |
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