KR900016757A - 유체 시료에서 변성 lgA₁의 검출 - Google Patents
유체 시료에서 변성 lgA₁의 검출 Download PDFInfo
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- KR900016757A KR900016757A KR1019890004882A KR890004882A KR900016757A KR 900016757 A KR900016757 A KR 900016757A KR 1019890004882 A KR1019890004882 A KR 1019890004882A KR 890004882 A KR890004882 A KR 890004882A KR 900016757 A KR900016757 A KR 900016757A
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- iga
- complex
- lectin
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- 239000012530 fluid Substances 0.000 title claims 15
- 238000001514 detection method Methods 0.000 title 1
- 108090000790 Enzymes Proteins 0.000 claims description 5
- 102000004190 Enzymes Human genes 0.000 claims description 5
- 206010028980 Neoplasm Diseases 0.000 claims description 3
- 201000011510 cancer Diseases 0.000 claims description 3
- 210000002966 serum Anatomy 0.000 claims description 3
- 101710186708 Agglutinin Proteins 0.000 claims description 2
- 101710146024 Horcolin Proteins 0.000 claims description 2
- 101710189395 Lectin Proteins 0.000 claims description 2
- 101710179758 Mannose-specific lectin Proteins 0.000 claims description 2
- 101710150763 Mannose-specific lectin 1 Proteins 0.000 claims description 2
- 101710150745 Mannose-specific lectin 2 Proteins 0.000 claims description 2
- 239000000910 agglutinin Substances 0.000 claims description 2
- 108090001090 Lectins Proteins 0.000 claims 15
- 102000004856 Lectins Human genes 0.000 claims 15
- 239000002523 lectin Substances 0.000 claims 15
- 239000007790 solid phase Substances 0.000 claims 14
- 238000000034 method Methods 0.000 claims 10
- 239000003550 marker Substances 0.000 claims 8
- 150000002482 oligosaccharides Chemical class 0.000 claims 6
- 229920001542 oligosaccharide Polymers 0.000 claims 5
- 238000005406 washing Methods 0.000 claims 4
- 229940088598 enzyme Drugs 0.000 claims 3
- 238000005259 measurement Methods 0.000 claims 3
- 108060003951 Immunoglobulin Proteins 0.000 claims 2
- 102000018358 immunoglobulin Human genes 0.000 claims 2
- 102000002260 Alkaline Phosphatase Human genes 0.000 claims 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 claims 1
- 240000003521 Bauhinia purpurea Species 0.000 claims 1
- 235000011462 Bauhinia purpurea Nutrition 0.000 claims 1
- 235000008733 Citrus aurantifolia Nutrition 0.000 claims 1
- 108010015776 Glucose oxidase Proteins 0.000 claims 1
- 239000004366 Glucose oxidase Substances 0.000 claims 1
- 108010001336 Horseradish Peroxidase Proteins 0.000 claims 1
- 108010046016 Peanut Agglutinin Proteins 0.000 claims 1
- 239000004793 Polystyrene Substances 0.000 claims 1
- 235000011941 Tilia x europaea Nutrition 0.000 claims 1
- 239000000427 antigen Substances 0.000 claims 1
- 102000036639 antigens Human genes 0.000 claims 1
- 108091007433 antigens Proteins 0.000 claims 1
- 239000011324 bead Substances 0.000 claims 1
- 239000013060 biological fluid Substances 0.000 claims 1
- 239000008280 blood Substances 0.000 claims 1
- 210000004369 blood Anatomy 0.000 claims 1
- 210000003022 colostrum Anatomy 0.000 claims 1
- 235000021277 colostrum Nutrition 0.000 claims 1
- 230000002950 deficient Effects 0.000 claims 1
- 230000002255 enzymatic effect Effects 0.000 claims 1
- 238000006911 enzymatic reaction Methods 0.000 claims 1
- 229940116332 glucose oxidase Drugs 0.000 claims 1
- 235000019420 glucose oxidase Nutrition 0.000 claims 1
- 239000004571 lime Substances 0.000 claims 1
- VYQNWZOUAUKGHI-UHFFFAOYSA-N monobenzone Chemical compound C1=CC(O)=CC=C1OCC1=CC=CC=C1 VYQNWZOUAUKGHI-UHFFFAOYSA-N 0.000 claims 1
- 210000002381 plasma Anatomy 0.000 claims 1
- 229920001184 polypeptide Polymers 0.000 claims 1
- 229920002223 polystyrene Polymers 0.000 claims 1
- 102000004196 processed proteins & peptides Human genes 0.000 claims 1
- 108090000765 processed proteins & peptides Proteins 0.000 claims 1
- 238000010791 quenching Methods 0.000 claims 1
- 239000007787 solid Substances 0.000 claims 1
- 239000000758 substrate Substances 0.000 claims 1
- 235000000346 sugar Nutrition 0.000 claims 1
- 150000008163 sugars Chemical class 0.000 claims 1
- -1 β-galactolidase Proteins 0.000 claims 1
- 238000003018 immunoassay Methods 0.000 description 2
- 238000003556 assay Methods 0.000 description 1
- 239000013610 patient sample Substances 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57469—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving tumor associated glycolinkage, i.e. TAG
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/811—Test for named disease, body condition or organ function
- Y10S436/813—Cancer
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/827—Lectins
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Hospice & Palliative Care (AREA)
- Oncology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
내용 없음.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 실시예1의 항-IgA-피넛 어글루티닌 효소 면역 분석을 사용하여 시험한 845혈청 표면의 패널(panel)로부터의 결과를 도시한 것이고,
제2도는 신규분석을 사용한 음성-CEA환자 시료 시험의 결과를 도시한 것이며,
제3도는 실시예2의 변성 항-IgA-피넛 어글루티닌 효소 면역 분석을 사용하여 정상환자, 비암환자 및 암환자의 180혈청 표본 시험의 결과를 도시한 것이다.
Claims (11)
- a) 유체 시료를 항-lgA 항체와 접촉시켜 항-IgA/lgA 복합체를 형성시키고 ; b) IgA1이 시료내에 존재할 경우 항-IgA/IgA 복합체를 IgA1에 대해 특이성을 지닌 표식렉틴과 접속시켜 항-IgA1/IgA1/렉틴 복합체를 형성시킨 다음 ; c) 유체 시료에서의 IgA1의 존재 또는 양의 척도로서 항-IgA/IgA1/렉틴 복합체에 대한 결합 또는비결합 표식의 존재 또는 양을 측정함을 특징으로 하여, 유체시료에서 0-결합 올리고 당류를 지닌 IgA1의 존재 또는 양을 측정하는 방법.
- a) 유체시료를 고체상에 결합된 항-IgA향체와 접촉시켜 항-IgA/IgA 고체상 복합체를 형성시키고, b) IgA1이 유체시료내에 존재할 경우 항-IgA/IgA 고체상 복합체를 IgA1에 대해 특이성을 지닌 표식레틴과 접촉시켜 항-IgA/IgA1/렉틴 고체 상 복합체를 형성시킨 다음 ; c) 유체 시료에서의 IgA1의 존재 또는 양의 척도로서 항-IgA/IgA1/렉틴 고체상 복합체에 대한 결합 또는 비결합 표식의 존재 또는 양을 측정함을 특징으로 하여, 유체시료에서 0-결합 올리고 당류를 지닌 IgA1의 존재 또는 양을 측정하는 방법.
- 제1항 또는 2항에 있어서, 표식을 효소, 방사성 동위원소 및 형광성 분자중에서 선택하는 방법.
- a) IgA 면역글로부린에 대해 특이적인 항체를 고체상에 흡수시키고 ; b) 유체시료를 항체-피복 고체상에 가하고 배양시켜 항-IgA/IgA 고체상 복합체를 형성 시키며 ; c) 복합체를 세척하여 미반응 시료를 제거하고 ; d) IgA1에 대해 특이성을 지닌 효소 표식렉틴을 가하고 배양시켜 항-IgA/IgA1/렉틴 고체상 복합체를 형성시키며 ; e) 항-IgA/IgA1/렉틴 고체상 복합체를 세척하고 ; f) 효소 기질을 가한 다음 배양시키며 ; 9) 효소 반응을 퀀치시키고 ; h) 시료내의 IgA1의 존재 또는 양의 척도로서 표식을 검출함을 특징으로 하여, 유체시료에서 0-결합올리고 당류를 지닌 IgA1의 존재 또는 양을 측정하는 방법.
- 제4항에 있어서, 효소표식을 알카리성 포스파타아제, β-갈락토올시다아제, 글루코스 옥시다아제 및 호스래디쉬 퍼옥시다아제 중에서 선택하는 방법.
- a) IgA 면역 글로부린에 대해 특이적인 항체를 고체상에 흡수시키고 ; b) 시료를 항체-피복 고체상에 가하여 항-IgA/IgA 고체상 복합체를 형성시키며 ; c) 복합체를 세척하여 미반응 시료를 제거하고 ; d) IgA1에 대해 특이성을 지닌 방사성 표식 렉틴을 가하며 ; e) 배양시켜 IgA1이 시료내에 존재할 경우 항-IgA/IgA1/렉틴 고체상 복합체를 형성시키고 ; f) 고체상을 세척하며 ; g) 시료내의 IgA1의 존재 또는 양의 척도로서 표식을 검출함을 특징으로 하여, 유체시료에서 변성된 0-결합 올리고 당류를 지닌 IgA1의 존재 또는 양을 측정하는 방법.
- 제 1,2,4 및 6항에 있어서, 유체 시료를 혈액, 혈청 및 혈장 중에서 선택하는 방법.
- 제 1,2.4 및 6항에 있어서, 렉틴을 피넛 어글루티닌(Peanut agglutinin) 또는 바우히니아 푸푸레아 알바(Bauhinia purpurea alba) 어글루티닌 중에서 선택하는 방법.
- 제 1,2,4 및 6항에 있어서, IgA1이 IgA1의 폴리펩티드 쇄에 대해 0-결합된 비차단되거나 노출된 DGaIBeta(1-3) DgalNac 올리고 당류 구조를 함유하는 방법.
- 항-인체/IgA 항체로 피복된 폴리스티렌 비두, IgA1에 대해 특이성을 지닌 표식렉틴, 표본회석 완충액 및 콜로스트럼 IgA 표준물질로 이루어진 유체시료에서 0-결할 올리고 당류를 지닌 IgA1을 시험하기 위한 키트(Kit).
- a) 유체시료를 항-IgA 항체와 접촉시켜 항-IgA/IgA 복합체를 형성시키고 ; b) IgA1이 시료내에 존재할 경우 항-IgA/IgA 복합체를 IgA1에 대해 특이성을 지 닌 표식렉틴과 접촉시켜 항-IgA/IgA1/렉틴 복합체를 형성시키며 ; c) 유체시료내의 IgA1의 존재 또는 양의 척도로서 항-IgA/IgA1/렉틴 복합체에 대해 결합 또는 비결합 표식의 존재 또는 양을 측정함을 특징으로 하여, 태생기암 항원 음성 생물학적 유체시료에서 0-결합 올리고 당류를 지닌 IgA1의 존재 또는 양을 측정하는 방법.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US181892 | 1988-04-15 | ||
| US07/181,892 US5051354A (en) | 1988-04-15 | 1988-04-15 | Detection of altered IGA1 in fluid samples |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR900016757A true KR900016757A (ko) | 1990-11-14 |
Family
ID=22666251
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1019890004882A KR900016757A (ko) | 1988-04-15 | 1989-04-13 | 유체 시료에서 변성 lgA₁의 검출 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US5051354A (ko) |
| EP (1) | EP0337410A3 (ko) |
| JP (1) | JPH01305356A (ko) |
| KR (1) | KR900016757A (ko) |
| AU (1) | AU623640B2 (ko) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5110911A (en) * | 1989-11-02 | 1992-05-05 | Biomira, Inc. | Human tumor-associated thomsen-friedenreich antigen |
| US5242799A (en) * | 1989-11-02 | 1993-09-07 | Biomira, Inc. | Lectin-antibody immunoassays for TF epitope-bearing antigens |
| DE19637418A1 (de) * | 1996-09-13 | 1998-03-19 | Boehringer Mannheim Gmbh | Homogene Nachweisverfahren zur Bestimmung von Subpopulationen eines Analyten |
| DE19856433C2 (de) * | 1998-12-08 | 2001-09-13 | Aventis Behring Gmbh | Verfahren zum spezifischen Nachweis von glykosilierten Proteinen |
| CN102043046B (zh) * | 2009-10-13 | 2014-05-07 | 张志丽 | 一种检测糖链异常IgA肾病的蛋白芯片 |
| US9016493B2 (en) | 2011-11-16 | 2015-04-28 | Cooksmith, Inc. | Baking apparatus with multiple functions and sizes |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES8102679A1 (es) * | 1979-01-30 | 1981-02-16 | Otsuka Pharma Co Ltd | Metodo de analizar fluidos corporales. |
| SE447028B (sv) * | 1980-06-30 | 1986-10-20 | Erik Audunn Cerven | Bestemning av endstaende sackaridgrupper i glykoprotein |
| JPS5729949A (en) * | 1980-07-30 | 1982-02-18 | Nippon Koutai Kenkyusho:Kk | Determination of sugar branch related to cancer and diagnostic technique of cancer |
| WO1987000289A1 (en) * | 1985-07-01 | 1987-01-15 | The Trustees Of Columbia University In The City Of | Lectin-antibody sandwich assay for desialylated glycoproteins |
-
1988
- 1988-04-15 US US07/181,892 patent/US5051354A/en not_active Expired - Fee Related
-
1989
- 1989-04-11 EP EP19890106443 patent/EP0337410A3/en not_active Withdrawn
- 1989-04-12 AU AU32709/89A patent/AU623640B2/en not_active Ceased
- 1989-04-13 KR KR1019890004882A patent/KR900016757A/ko active IP Right Grant
- 1989-04-13 JP JP1096200A patent/JPH01305356A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| US5051354A (en) | 1991-09-24 |
| AU623640B2 (en) | 1992-05-21 |
| AU3270989A (en) | 1989-10-19 |
| EP0337410A3 (en) | 1990-10-17 |
| JPH01305356A (ja) | 1989-12-08 |
| EP0337410A2 (en) | 1989-10-18 |
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