KR890014749A - 미생물 검출 장치 및 기기 - Google Patents

미생물 검출 장치 및 기기 Download PDF

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KR890014749A
KR890014749A KR1019890003105A KR890003105A KR890014749A KR 890014749 A KR890014749 A KR 890014749A KR 1019890003105 A KR1019890003105 A KR 1019890003105A KR 890003105 A KR890003105 A KR 890003105A KR 890014749 A KR890014749 A KR 890014749A
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sample
sensor
medium
indicator
vessel
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KR1019890003105A
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KR970003150B1 (ko
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에드워드 튜너 제임스
클레이 쏘르페 터어만
로오렌스 디귀세피 제임스
코르넬리우스 드리스콜 리챠드
존 칼란드라 미카엘
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에이.쥐.제이.베르메텐·에프.위.엠.헤르만스
악조 엔. 브이
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
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  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

내용 없음.

Description

미생물 검출 장치 및 기기
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 혈액 배양 기계를 도시한 것이고 ;
제2도는 pH 감수성을 도시한 것이며 ;
제3도는 미생물 성장에 따른 pH 및 CO2변화를 도시한 것이다.

Claims (10)

  1. 미생물에 의한 표본의 오염여부를 검출할 수 있도록 표본과 멸균 배양배지를 함께 배양할 수 있는 내실을 가지며, 또한 용기의 벽에 적어도 일부분의 투명한 단면을 갖는 밀폐시킬 수 있고, 멸균처리가능한 표본용기와 상기 용기의 내벽 표면에 부착된 센서로 구성되어 용기의 외부에서 상술한 투명한 단면을 통해서 센서에 나타나는 변화를 검출할 수 있으며, 상기 센서는 고정시킨 지시제 매질을 포함하고, 이 지시제 매질은 생물체의 대사활성 생성물들에 노출시킬 경우 검출 가능한 변화를 나타내는 자체의 기능에 따라 선택되며, 상기 지시제 매질이 지지체를 지지체 매질과 결합시키거나 또는 지시제 중합체 매트릭스 내부에 캡슐화시키는 방법에 의해 고정되어 있는 표본내의 미생물 검출용 장치.
  2. 제1항에 있어서, 지시제 매질이 pH에 대하여 반응하는 분자종인 장치.
  3. 제1항에 있어서, 센서가 전하를 띈 멤브레인을 포함하는 장치.
  4. 제1항에 있어서, 센서의 멤브레인이 상기 용기의 내부 표면에 결합되어 있는 장치.
  5. 제4항에 있어서, 센서의 멤브레인을 용기의 내부표면에 적어도 원형으로 결합시킴으로써 표본 및 배양배지 둘다 멤브레인과 용기 표면 사이를 통과할 수 없도록 한 장치.
  6. 제1항에 있어서, 반 투과성 멤브레인을 이용하여 상기 용기의 표본으로부터 센서를 분리시킨 장치.
  7. 제6항에 있어서, 반투과성 멤브레인이 즉석에서 형성된 멤브레인의 장치.
  8. 제1항에 있어서, 센서가 중합체 매트릭스 내부에 고정시킨 지시제 매질의 마이셀들을 포함하는 장치.
  9. 제8항에 있어서, 지시제 용액을 경화시키지 않은 중합체 내부에 유화시키고, 이어서 중합체를 경화시키는 방법으로 지시제 매질의 마이셀들을 중합체 매트릭스 내부에 형성시킨 장치.
  10. 자체의 벽에 적어도 일부분의 투명한 단면, 및 상기 투명한 단면부분에의 용기 내벽 표면에 부착된, 생물체들의 대사활성에 의한 기체 생성물등에 노출시킬 경우 검출 가능한 변화를 나타내는 지시제 매질을 함유하는 센서를 갖는 멸균시킨 표본용기내에 멸균조건들하에서 표본을 도입하는 단계 ; 센서가 표본 및 성장배지 하부에 완전히 침지되도록 하는 위치로 표본용기를 배향시킨 상태에서, 표본 용기내의 표본을 미생물 성장배지와 함께 배양하는 단계 ; 및 표본 및 성장배지로부터 센서로 통과하는 기체 생성물들에 의해 센서의 지시제 매질에 나타나는 변화를 검출함으로써, 온전한 용기를 손상시킴이 없이 용기의 외부에서 자체의 투명한 단면을 통해서 생물체들의 대사활성에 기인된 기체 생성물들의 생산을 점검하는 단계들로 구성되는, 표본내의 매생물 검출방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019890003105A 1988-03-15 1989-03-14 미생물 검출 장치 및 기기 KR970003150B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US07/168,291 US4945060A (en) 1988-03-15 1988-03-15 Device for detecting microorganisms
US07/168,291 1988-03-15
NL07/168291 1988-03-15

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KR890014749A true KR890014749A (ko) 1989-10-25
KR970003150B1 KR970003150B1 (ko) 1997-03-14

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US (1) US4945060A (ko)
EP (1) EP0333253B1 (ko)
JP (1) JP2862556B2 (ko)
KR (1) KR970003150B1 (ko)
AT (1) ATE126267T1 (ko)
AU (1) AU631533B2 (ko)
CA (1) CA1339512C (ko)
DE (2) DE333253T1 (ko)
DK (1) DK123889A (ko)
ES (1) ES2031807T3 (ko)
GR (1) GR3017974T3 (ko)
IE (1) IE67634B1 (ko)
ZA (1) ZA891788B (ko)

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DK123889D0 (da) 1989-03-14
ES2031807T3 (es) 1995-12-16
IE890746L (en) 1989-09-15
KR970003150B1 (ko) 1997-03-14
DE333253T1 (de) 1993-02-25
EP0333253B1 (en) 1995-08-09
ES2031807T1 (es) 1993-01-01
AU631533B2 (en) 1992-12-03
ZA891788B (en) 1990-03-28
EP0333253A3 (en) 1990-01-17
DK123889A (da) 1989-09-16
ATE126267T1 (de) 1995-08-15
US4945060A (en) 1990-07-31
JP2862556B2 (ja) 1999-03-03
IE67634B1 (en) 1996-04-17
DE68923720T2 (de) 1996-02-08
JPH0216965A (ja) 1990-01-19
CA1339512C (en) 1997-10-28
AU3128889A (en) 1989-09-21
EP0333253A2 (en) 1989-09-20
GR3017974T3 (en) 1996-02-29
DE68923720D1 (de) 1995-09-14

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